NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13391R-4

Symbol Aats-ala Full Name Alanyl-tRNA synthetase

CG No CG13391 Old CG No CG13391

Synonyms AlaRS, alaS, ARS, CG13391, Aats-ala

Accession No (Link to NCBI) NM_078787.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Yano H, Yamamoto-Hino M, Awano W, Aoki-Kinoshita KF, Tsuda-Sakurai K, Okano H, Goto S.
Identification of proteasome components required for apical localization of Chaoptin using functional genomics.
J Neurogenet (2012) 26(1) 53-63 [ PubMed ID = 22417167 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACTCACAGCAAAGGAGGTGCG

Primer Seq. 3'

aatctagaggtaccTGCTGCCCGGCAGGATGTGTT

Predicted Fragment Size

IR fragment full Seq

0001 ACTCACAGCA AAGGAGGTGC GCAACGCCTA TCTGGACTTC TTCAAGGAGC AAGAAGCACA 
0061 TCTATGTTCA CTCGTCCAGC ACGATTCCAT TGGACGATCC CACGCTGTTG TTCGCCAACG 
0121 CCGGCATGAA TCAGTTCAAG CCCATTTTTC TGGGCACCGC TGATCCCAAC AGCGAAATGT 
0181 CCAAGTGGGT GCGCGTGGCC AACACGCAGA AGTGCATTCG CGCCGGTGGC AAGCACAACG 
0241 ATCTGGACGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAAATG CTGGGAAACT 
0301 GGTCCTTCGG TGACTACTTT AAGAAGGAGA TCTGCTCCTG GGCCTGGGAG TTTCTCACGC 
0361 AGCGACTCGC CCTACCCAAG GATCGTCTGT ATGTGACTTA CTTTGGTGGT GACGCGGCCA 
0421 GCGGTCTTGG AGCCCGATCT GGAGTGCAAG CAAATGTGGC TGGACTTGGG CCTGAAACCG 
0481 GAACACATCC TGCCGGGCAG CA

in silico PCR Fragment

0001 ACTCACAGCA AAGGAGGTGC GCAACGCCTA TCTGGACTTC TTCAAGGAGC -AGAAGCACA 
0061 TCTATGTTCA CTCGTCCAGC ACGATTCCAT TGGACGATCC CACGCTGTTG TTCGCCAACG 
0121 CCGGCATGAA TCAGTTCAAG CCCATTTTTC TGGGCACCGC TGATCCCAAC AGCGAAATGT 
0181 CCAAGTGGGT GCGCGTGGCC AACACGCAGA AGTGCATTCG CGCCGGTGGC AAGCACAACG 
0241 ATCTGGACGA CGTGGGCAAG GATGTCTATC ATCACACCTT CTTTGAAATG CTGGGAAACT 
0301 GGTCCTTCGG TGACTACTTT AAGAAGGAGA TCTGCTCCTG GGCCTGGGAG TTTCTCACGC 
0361 AGCGACTCGC CCTACCCAAG GATCGTCTGT ATGTGACTTA CTTTGGTGGT GACGCGGCCA 
0421 GCGGTC-TGG AGCCCGATCT GGAGTGCAAG CAAATGTGGC TGGACTTGGG CCTGAAACCG 
0481 GAACACATCC TGCCGGGCAG CA

Assemble Data


13391R-4.IR_full       1   ACTCACAGCAAAGGAGGTGCGCAACGCCTATCTGGACTTCTTCAAGGAGCAAGAAGCACA 60
                           |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
13391R-4.in silico     1   ACTCACAGCAAAGGAGGTGCGCAACGCCTATCTGGACTTCTTCAAGGAGC-AGAAGCACA 60


13391R-4.IR_full       61  TCTATGTTCACTCGTCCAGCACGATTCCATTGGACGATCCCACGCTGTTGTTCGCCAACG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13391R-4.in silico     61  TCTATGTTCACTCGTCCAGCACGATTCCATTGGACGATCCCACGCTGTTGTTCGCCAACG 120


13391R-4.IR_full       121 CCGGCATGAATCAGTTCAAGCCCATTTTTCTGGGCACCGCTGATCCCAACAGCGAAATGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13391R-4.in silico     121 CCGGCATGAATCAGTTCAAGCCCATTTTTCTGGGCACCGCTGATCCCAACAGCGAAATGT 180


13391R-4.IR_full       181 CCAAGTGGGTGCGCGTGGCCAACACGCAGAAGTGCATTCGCGCCGGTGGCAAGCACAACG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13391R-4.in silico     181 CCAAGTGGGTGCGCGTGGCCAACACGCAGAAGTGCATTCGCGCCGGTGGCAAGCACAACG 240


13391R-4.IR_full       241 ATCTGGACGACGTGGGCAAGGATGTCTATCATCACACCTTCTTTGAAATGCTGGGAAACT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13391R-4.in silico     241 ATCTGGACGACGTGGGCAAGGATGTCTATCATCACACCTTCTTTGAAATGCTGGGAAACT 300


13391R-4.IR_full       301 GGTCCTTCGGTGACTACTTTAAGAAGGAGATCTGCTCCTGGGCCTGGGAGTTTCTCACGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13391R-4.in silico     301 GGTCCTTCGGTGACTACTTTAAGAAGGAGATCTGCTCCTGGGCCTGGGAGTTTCTCACGC 360


13391R-4.IR_full       361 AGCGACTCGCCCTACCCAAGGATCGTCTGTATGTGACTTACTTTGGTGGTGACGCGGCCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13391R-4.in silico     361 AGCGACTCGCCCTACCCAAGGATCGTCTGTATGTGACTTACTTTGGTGGTGACGCGGCCA 420


13391R-4.IR_full       421 GCGGTCTTGGAGCCCGATCTGGAGTGCAAGCAAATGTGGCTGGACTTGGGCCTGAAACCG 480
                           |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
13391R-4.in silico     421 GCGGTC-TGGAGCCCGATCTGGAGTGCAAGCAAATGTGGCTGGACTTGGGCCTGAAACCG 480


13391R-4.IR_full       481 GAACACATCCTGCCGGGCAGCA 502
                           ||||||||||||||||||||||
13391R-4.in silico     481 GAACACATCCTGCCGGGCAGCA 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_078787.2	CG13391-RA, transcript variant A (Aats-ala), mRNA
100	482	0	0	0	NM_205934.1	CG13391-RB, transcript variant B (Aats-ala), mRNA
0.41	2	3	3	5	NM_001014610.1	CG33546-RB, transcript variant B (gfzf), mRNA
0	0	13	37	19	NM_079208.2	CG4633-RA (Aats-ala-m), mRNA
0	0	5	2	3	NM_080156.2	CG4535-RA (FKBP59), mRNA
0	0	3	4	2	NM_134555.1	CG15454-RA (CG15454), mRNA
0	0	3	3	13	NM_132097.1	CG3950-RA (CG3950), mRNA
0	0	2	3	7	NM_079176.1	CG12008-RA, transcript variant A (kst), mRNA
0	0	2	3	7	NM_206266.1	CG12008-RB, transcript variant B (kst), mRNA
0	0	2	3	7	NM_206265.1	CG12008-RC, transcript variant C (kst), mRNA
0	0	2	3	3	NM_170420.1	CG18741-RA, transcript variant A (DopR2), mRNA
0	0	2	3	3	NM_079824.2	CG18741-RB, transcript variant B (DopR2), mRNA
0	0	2	3	2	NM_134846.2	CG3227-RA (insv), mRNA
0	0	2	3	2	NM_206662.1	CG12065-RC, transcript variant C (CG12065), mRNA
0	0	2	3	2	NM_167162.2	CG12065-RB, transcript variant B (CG12065), mRNA
0	0	2	3	2	NM_206661.1	CG12065-RD, transcript variant D (CG12065), mRNA
0	0	2	3	2	NM_132265.2	CG12065-RA, transcript variant A (CG12065), mRNA
0	0	2	2	3	NM_175945.1	CG4297-RB, transcript variant B (CG4297), mRNA
0	0	2	2	3	NM_134683.1	CG4297-RA, transcript variant A (CG4297), mRNA
0	0	2	2	3	NM_164454.1	CG31671-RA (tho2), mRNA
0	0	1	5	9	NM_167285.2	CG11727-RA, transcript variant A (CG11727), mRNA
0	0	1	5	9	NM_132488.3	CG11727-RB, transcript variant B (CG11727), mRNA
0	0	1	5	4	NM_137272.1	CG3896-RA (Nox), mRNA
0	0	1	4	6	NM_132642.2	CG15744-RA (CG15744), mRNA
0	0	1	4	3	NM_136253.2	CG9241-RA (Mcm10), mRNA
0	0	1	3	2	NM_168196.1	CG32387-RA, transcript variant A (CG32387), mRNA
0	0	1	3	2	NM_168195.1	CG32387-RB, transcript variant B (CG32387), mRNA
0	0	1	3	2	NM_001043123.1	CG32387-RC, transcript variant C (CG32387), mRNA
0	0	1	2	6	NM_079821.2	CG1954-RA (Pkc98E), mRNA
0	0	1	2	5	NM_136688.1	CG12923-RA (CG12923), mRNA

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