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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13343R-4 |
| Symbol |
CG13343 |
Full Name |
CG13343 |
| CG No |
CG13343 |
Old CG No |
CG13343 |
| Synonyms |
CG13343 |
| Accession No (Link to NCBI) |
NM_137069.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Strutt H, Searle E, Thomas-Macarthur V, Brookfield R, Strutt D.
A Cul-3-BTB ubiquitylation pathway regulates junctional levels and asymmetry of core planar polarity proteins.
Development (2013) 140(8) 1693-702
[
PubMed ID = 23487316
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCCACTCACCCAATCCTGGCT |
| Primer Seq. 3' |
aatctagaggtaccGGTGCCGTCCTCCTCATATCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TCCACTCACC CAATCCTGGC TTAATCCTCC AGAGCAAGCG TTTCAATGGA CTGCGGAACA
0061 TCCTGGAACG CGAAGGTCCC TTCTGCAAGG ACGGTTTCGC GGCCTCGTCG GAAAACCTGG
0121 AGTTCTTGCA GACCAAGTGC CAGGTTCTGA TCATCGGAGC CGGCGGCCTG GGCTGCGAAC
0181 TGCTGAAGGA TCTGGCTCTG ATGGGCTTCG GCAACCTGCA CGTCATCGAC ATGGACACCA
0241 TCGAGCTGTC CAACCTGAAT CGCCAGTTTC TGTTCCGTCG CACCGACATT GGCGCCTCCA
0301 AGGCGGAGTG TGCGGCTCGC TTCATCAACG CAAGAGTGCC TACTTGCCGG GTGACGCCGC
0361 ATTTCAAGAA GATCCAGGAC TTCGATGAGT CCTTTTATCA GCAGTTCCAC CTGGTTGNCT
0421 GTGGCCTAGA CTCCATTGTC GCCCGGCGTT GGATCAATGG NATGCTGCTC TCCATGCTGC
0481 GATATGAGGA GGACGGCACC |
| in silico PCR Fragment |
0001 TCCACTCACC CAATCCTGGC TTAATCCTCC AGAGCAAGCG TTTCAATGGA CTGCGGAACA
0061 TCCTGGAACG CGAAGGTCCC TTCTGCAAGG ACGGTTTCGC GGCCTCGTCG GAAAACCTGG
0121 AGTTCTTGCA GACCAAGTGC CAGGTTCTGA TCATCGGAGC CGGTGGCCTG GGCTGCGAAC
0181 TGCTGAAGGA TCTGGCTCTG ATGGGCTTCG GCAACCTGCA CGTCATCGAC ATGGACACCA
0241 TCGAGCTGTC CAACCTGAAT CGCCAGTTTC TGTTCCGTCG CACAGACATT GGCGCCTCCA
0301 AGGCGGAGTG TGCGGCTCGC TTCATCAACG CAAGAGTGCC TACTTGCCGG GTGACGCCGC
0361 ATTTCAAGAA AATCCAGGAC TTCGATGAGT CCTTCTATCA GCAGTTCCAC CTGGTTGTCT
0421 GTGGCCTAGA CTCCATTGTC GCCCGGCGTT GGATCAATGG CATGCTGCTA TCAATGCTGC
0481 GATATGAGGA GGACGGCACC |
| Assemble Data |
13343R-4.IR_full 1 TCCACTCACCCAATCCTGGCTTAATCCTCCAGAGCAAGCGTTTCAATGGACTGCGGAACA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13343R-4.in silico 1 TCCACTCACCCAATCCTGGCTTAATCCTCCAGAGCAAGCGTTTCAATGGACTGCGGAACA 60
13343R-4.IR_full 61 TCCTGGAACGCGAAGGTCCCTTCTGCAAGGACGGTTTCGCGGCCTCGTCGGAAAACCTGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13343R-4.in silico 61 TCCTGGAACGCGAAGGTCCCTTCTGCAAGGACGGTTTCGCGGCCTCGTCGGAAAACCTGG 120
13343R-4.IR_full 121 AGTTCTTGCAGACCAAGTGCCAGGTTCTGATCATCGGAGCCGGCGGCCTGGGCTGCGAAC 180
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
13343R-4.in silico 121 AGTTCTTGCAGACCAAGTGCCAGGTTCTGATCATCGGAGCCGGTGGCCTGGGCTGCGAAC 180
13343R-4.IR_full 181 TGCTGAAGGATCTGGCTCTGATGGGCTTCGGCAACCTGCACGTCATCGACATGGACACCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13343R-4.in silico 181 TGCTGAAGGATCTGGCTCTGATGGGCTTCGGCAACCTGCACGTCATCGACATGGACACCA 240
13343R-4.IR_full 241 TCGAGCTGTCCAACCTGAATCGCCAGTTTCTGTTCCGTCGCACCGACATTGGCGCCTCCA 300
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
13343R-4.in silico 241 TCGAGCTGTCCAACCTGAATCGCCAGTTTCTGTTCCGTCGCACAGACATTGGCGCCTCCA 300
13343R-4.IR_full 301 AGGCGGAGTGTGCGGCTCGCTTCATCAACGCAAGAGTGCCTACTTGCCGGGTGACGCCGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13343R-4.in silico 301 AGGCGGAGTGTGCGGCTCGCTTCATCAACGCAAGAGTGCCTACTTGCCGGGTGACGCCGC 360
13343R-4.IR_full 361 ATTTCAAGAAGATCCAGGACTTCGATGAGTCCTTTTATCAGCAGTTCCACCTGGTTGNCT 420
|||||||||| ||||||||||||||||||||||| |||||||||||||||||||||| ||
13343R-4.in silico 361 ATTTCAAGAAAATCCAGGACTTCGATGAGTCCTTCTATCAGCAGTTCCACCTGGTTGTCT 420
13343R-4.IR_full 421 GTGGCCTAGACTCCATTGTCGCCCGGCGTTGGATCAATGGNATGCTGCTCTCCATGCTGC 480
|||||||||||||||||||||||||||||||||||||||| |||||||| || |||||||
13343R-4.in silico 421 GTGGCCTAGACTCCATTGTCGCCCGGCGTTGGATCAATGGCATGCTGCTATCAATGCTGC 480
13343R-4.IR_full 481 GATATGAGGAGGACGGCACC 500
||||||||||||||||||||
13343R-4.in silico 481 GATATGAGGAGGACGGCACC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_137069.2 |
CG13343-RA (CG13343), mRNA |
| 0.2 |
1 |
5 |
4 |
8 |
NM_205912.1 |
CG11020-RB, transcript variant B (nompC), mRNA |
| 0.2 |
1 |
5 |
4 |
8 |
NM_078759.2 |
CG11020-RA, transcript variant A (nompC), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_169912.1 |
CG4058-RB, transcript variant B (Nep4), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_142647.2 |
CG4058-RA, transcript variant A (Nep4), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_166291.1 |
CG30116-RD, transcript variant D (CG30116), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_166292.1 |
CG30116-RA, transcript variant A (CG30116), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137494.1 |
CG30116-RB, transcript variant B (CG30116), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137493.2 |
CG30116-RC, transcript variant C (CG30116), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_168383.1 |
CG6718-RB, transcript variant B (CG6718), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_168385.1 |
CG6718-RD, transcript variant D (CG6718), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_140109.1 |
CG6718-RA, transcript variant A (CG6718), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_168384.1 |
CG6718-RC, transcript variant C (CG6718), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_136864.2 |
CG13185-RA (CG13185), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_078982.2 |
CG8344-RA (RpIII128), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_141666.2 |
CG9492-RA (CG9492), mRNA |
| 0 |
0 |
1 |
8 |
3 |
NM_206045.1 |
CG3572-RC, transcript variant C (vimar), mRNA |
| 0 |
0 |
1 |
8 |
3 |
NM_057957.4 |
CG3572-RB, transcript variant B (vimar), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_057837.3 |
CG1616-RA (dpa), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_133114.2 |
CG32541-RA (CG32541), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_165148.1 |
CG31819-RA (CG31819), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_057784.4 |
CG7833-RA (Orc5), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141394.2 |
CG10284-RA (CG10284), mRNA |
| 0 |
0 |
0 |
8 |
5 |
NM_132064.2 |
CG4666-RA (CG4666), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_137423.1 |
CG18635-RA (CG18635), mRNA |
| 0 |
0 |
0 |
5 |
12 |
NM_166525.1 |
CG3413-RC, transcript variant C (wdp), mRNA |
| 0 |
0 |
0 |
5 |
12 |
NM_166524.1 |
CG3413-RA, transcript variant A (wdp), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
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| VM |
 |
| + |
|
| VB |
 |
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
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