NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13319R-2

Symbol CG13319 Full Name CG13319

CG No CG13319 Old CG No CG13319

Synonyms BcDNA:GH19585, CG13319

Accession No (Link to NCBI)

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564 [ PubMed ID = 34239451 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGATAGCGTTCCTGCGAAT

Primer Seq. 3'

aatctagaggtaccGAAGTGCTCCAGATGGTCTTG

Predicted Fragment Size

IR fragment full Seq

in silico PCR Fragment

Assemble Data


13319R-2.IR full       1   ACGGATAGCGTTCCTGCGAATGTTGTGCCTGCGAAGGAGTCTGGTGGCTTCACCTCACAT 60
                           | ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
13319R-2.in silico     1   ATGGATAGCGTTCCTGCGAATGTTGTGCCTGCGAAGGAGTCTGGTGGTTTCACCTCACAT 60


13319R-2.IR full       61  TGCGTCCACTTGGCGGCGGGNCCCTCGCAATTCACCGTGCGCGCCCTGAAAATGCAAGGC 120
                           |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
13319R-2.in silico     61  TGCGTCCACTTGGCGGCGGGTCCCTCGCAATTCACCGTGCGCGCCCTGAAAATGCAAGGC 120


13319R-2.IR full       121 AGCACCATGCTGATCATAAACCCCAAGGGATTGGAGGTTTTCGAGGAGCTAGCGGTGGCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13319R-2.in silico     121 AGCACCATGCTGATCATAAACCCCAAGGGATTGGAGGTTTTCGAGGAGCTAGCGGTGGCC 180


13319R-2.IR full       181 ATGCCCTCTCGCAATCCCGCCAGCAGCCAGTCCCTATCCTCCACAATCCTGGGCGGACAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13319R-2.in silico     181 ATGCCCTCTCGCAATCCCGCCAGCAGCCAGTCCCTATCCTCCACAATCCTGGGCGGACAC 240


13319R-2.IR full       241 GGGCAGACCGACTCATCTGTTCTGGCCGCCAAATTGAGTAAACGCTACTGCCGCCAGTTC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13319R-2.in silico     241 GGGCAGACCGACTCATCTGTTCTGGCCGCCAAATTGAGTAAACGCTACTGCCGCCAGTTC 300


13319R-2.IR full       301 TACGTGAGCCTTAATCTCAAACTGGATCGATTAATGGGCCCTCTGTTCGAGAAGACGCTA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13319R-2.in silico     301 TACGTGAGCCTTAATCTCAAACTGGATCGATTAATGGGCCCTCTGTTCGAGAAGACGCTA 360


13319R-2.IR full       361 GTCACCTACATGCAAGACCATCTGGAGCACTTC 393
                           |||||||||||||||||||||||||||||||||
13319R-2.in silico     361 GTCACCTACATGCAAGACCATCTGGAGCACTTC 393

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

Wing Disc