National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13295R-3 
 Symbol CG13295  Full Name CG13295 
 CG No CG13295  Old CG No CG13295 
 Synonyms CG13295 
 Accession No (Link to NCBI) NM_139764.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGGCTTTGTATGAACGCAGAC 
 Primer Seq. 3' 
aatctagaggtaccACAGCCACGGTAGTGCTCAAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGGCTTTGTA TGAACGCAGA CAGCGAAGCG GGGACACCCA AGTGCTCAAG TTCTATGTAC 
0061 GCACCAGTTC AGATGCTGAC AAGCGAGACT CCTTCAAGCT CATCCAAATA CGCACGGACT 
0121 GGCAGAGTAC CCGAGATCCC AAAACCTTTA TTCAAGGTCT TCTGGAGTCA TGCAACTTGT 
0181 CCACGGAGTA TGTGAAGAAC ATTAAGGCCC AGCCTGAGCC GCCAAAAACC TCTGGCTTGG 
0241 ATTTTAAACC AAGCCATGAC TATCACTATG ACATGGAGTT CGCCGAGTTT GAAACTCAGT 
0301 TGAAGAATGA ACGTGCCAGT AAGCGTCCAG TGCTGACCAC CTGGCTGGAA GAGAATNGCT 
0361 GGCACAGCCA AACAGCGCTC TAAGGAAACT GCGGATCTGC AGGCCGAAAT CGACAAACTG 
0421 AAAAAAGTGC TGGTAGAGAA ACTGGAGTTG CAAGATGCTC GTTACGAGTG CGGCTGGAAC 
0481 ATTGAGcaCT ACCGTGGCTG T 
 in silico PCR Fragment 
0001 AGGCTTTGTA TGAACGCAGA CAGCGAAGCG GGGACACCCA AGTGCTCAAG TTCTATGTAC 
0061 GCACCAGTTC AGATGCTGAC AAGCGAGACT CCTTCAAGCT CATCCAAATA CGCACGGACT 
0121 GGCAGAGTAC CCGAGATCCC AAAACCTTTA TTCAAGGTCT TCTGGAGTCA TGCAACTTGT 
0181 CCACGGAGTA TGTGAAGAAC ATTAAGGCCC AGCCTGAGCC GCCAAAAACC TCTGGCTTGG 
0241 ATTTTAAACC AAGCCATGAC TATCACTATG ACATGGAGTT CGCCGAGTTT GAAACTCAGT 
0301 TGAAGAATGA ACGTGCCAGT AAGCGTCCAG TGCTGACCAC CTGGCTGGAA GAGAAT-GCT 
0361 GGCACAGCCA AACAGCGCTC TAAGGAAACT GCGGATCTGC AGGCCGAAAT CGACAAACTG 
0421 AAAAAAATGC TGGTAGAGAA ACTGGAGTTG CAAGATGCTC GTTACGAGTG CGGCTGGAAC 
0481 ATTGAGCACT ACCGTGGCTG T 
 Assemble Data 

13295R-3.IR_full       1   AGGCTTTGTATGAACGCAGACAGCGAAGCGGGGACACCCAAGTGCTCAAGTTCTATGTAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13295R-3.in silico     1   AGGCTTTGTATGAACGCAGACAGCGAAGCGGGGACACCCAAGTGCTCAAGTTCTATGTAC 60


13295R-3.IR_full       61  GCACCAGTTCAGATGCTGACAAGCGAGACTCCTTCAAGCTCATCCAAATACGCACGGACT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13295R-3.in silico     61  GCACCAGTTCAGATGCTGACAAGCGAGACTCCTTCAAGCTCATCCAAATACGCACGGACT 120


13295R-3.IR_full       121 GGCAGAGTACCCGAGATCCCAAAACCTTTATTCAAGGTCTTCTGGAGTCATGCAACTTGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13295R-3.in silico     121 GGCAGAGTACCCGAGATCCCAAAACCTTTATTCAAGGTCTTCTGGAGTCATGCAACTTGT 180


13295R-3.IR_full       181 CCACGGAGTATGTGAAGAACATTAAGGCCCAGCCTGAGCCGCCAAAAACCTCTGGCTTGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13295R-3.in silico     181 CCACGGAGTATGTGAAGAACATTAAGGCCCAGCCTGAGCCGCCAAAAACCTCTGGCTTGG 240


13295R-3.IR_full       241 ATTTTAAACCAAGCCATGACTATCACTATGACATGGAGTTCGCCGAGTTTGAAACTCAGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13295R-3.in silico     241 ATTTTAAACCAAGCCATGACTATCACTATGACATGGAGTTCGCCGAGTTTGAAACTCAGT 300


13295R-3.IR_full       301 TGAAGAATGAACGTGCCAGTAAGCGTCCAGTGCTGACCACCTGGCTGGAAGAGAATNGCT 360
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
13295R-3.in silico     301 TGAAGAATGAACGTGCCAGTAAGCGTCCAGTGCTGACCACCTGGCTGGAAGAGAAT-GCT 360


13295R-3.IR_full       361 GGCACAGCCAAACAGCGCTCTAAGGAAACTGCGGATCTGCAGGCCGAAATCGACAAACTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13295R-3.in silico     361 GGCACAGCCAAACAGCGCTCTAAGGAAACTGCGGATCTGCAGGCCGAAATCGACAAACTG 420


13295R-3.IR_full       421 AAAAAAGTGCTGGTAGAGAAACTGGAGTTGCAAGATGCTCGTTACGAGTGCGGCTGGAAC 480
                           |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
13295R-3.in silico     421 AAAAAAATGCTGGTAGAGAAACTGGAGTTGCAAGATGCTCGTTACGAGTGCGGCTGGAAC 480


13295R-3.IR_full       481 ATTGAGCACTACCGTGGCTGT 501
                           |||||||||||||||||||||
13295R-3.in silico     481 ATTGAGCACTACCGTGGCTGT 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_139764.2  CG13295-RA (CG13295), mRNA 
0   0  4  3  3  NM_134677.1  CG13690-RA (CG13690), mRNA 
0   0  2  3  2  NM_057742.3  CG3269-RA (Rab2), mRNA 
0   0  1  3  2  NM_058133.3  CG31426-RA (ligatin), mRNA 
0   0  1  2  6  NM_206164.1  CG5170-RA, transcript variant A (Dp1), mRNA 
0   0  1  2  5  NM_166282.1  CG5170-RF, transcript variant F (Dp1), mRNA 
0   0  1  2  5  NM_206163.1  CG5170-RB, transcript variant B (Dp1), mRNA 
0   0  1  2  5  NM_166281.1  CG5170-RE, transcript variant E (Dp1), mRNA 
0   0  1  2  5  NM_166280.1  CG5170-RD, transcript variant D (Dp1), mRNA 
0   0  1  2  5  NM_079057.2  CG5170-RC, transcript variant C (Dp1), mRNA 
0   0  1  2  4  NM_137043.2  CG13333-RA (CG13333), mRNA 
0   0  1  2  3  NM_134747.2  CG5397-RA (CG5397), mRNA 
0   0  1  2  2  NM_169909.1  CG5460-RB, transcript variant B (H), mRNA 
0   0  1  2  2  NM_169908.1  CG5460-RD, transcript variant D (H), mRNA 
0   0  1  2  2  NM_169907.1  CG5460-RA, transcript variant A (H), mRNA 
0   0  1  2  2  NM_079694.2  CG5460-RC, transcript variant C (H), mRNA 
0   0  0  5  10  NM_001042904.1  CG15148-RB, transcript variant B (btv), mRNA 
0   0  0  5  10  NM_001042905.1  CG15148-RC, transcript variant C (btv), mRNA 
0   0  0  5  10  NM_136013.1  CG15148-RA, transcript variant A (btv), mRNA 
0   0  0  5  5  NM_058026.3  CG5709-RA (ari-2), mRNA 
0   0  0  5  4  NM_132415.1  CG9806-RA (CG9806), mRNA 
0   0  0  4  5  NM_170580.2  CG2098-RB, transcript variant B (ferrochelatase), mRNA 
0   0  0  4  5  NM_170579.2  CG2098-RC, transcript variant C (ferrochelatase), mRNA 
0   0  0  4  5  NM_079874.2  CG2098-RA, transcript variant A (ferrochelatase), mRNA 
0   0  0  4  5  NM_167841.1  CG32341-RA (CG32341), mRNA 
0   0  0  4  4  NM_138100.2  CG4707-RA (CG4707), mRNA 
0   0  0  4  4  NM_168218.1  CG32372-RA (CG32372), mRNA 
0   0  0  4  2  NM_142075.1  CG14358-RA (CG14358), mRNA 
0   0  0  3  7  NM_057969.3  CG9635-RD, transcript variant D (RhoGEF2), mRNA 
0   0  0  3  7  NM_206146.1  CG9635-RF, transcript variant F (RhoGEF2), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.