|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1322R-2 |
| Symbol |
zfh1 |
Full Name |
Zn finger homeodomain 1 |
| CG No |
CG1322 |
Old CG No |
CG1322 |
| Synonyms |
Zfh-1, ZFH-1, zfh-1, Zfh1, zfl-1, l(3)00865, CG1322, zfh1, ZFH1, Zfh1a |
| Accession No (Link to NCBI) |
NM_170523.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Enomoto M, Takemoto D, Igaki T.
Interaction between Ras and Src clones causes interdependent tumor malignancy via Notch signaling in Drosophila.
Dev Cell (2021) 56(15) 2223-2236.e5
[
PubMed ID = 34324859
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGACCTGCCGCATCTGCCACA |
| Primer Seq. 3' |
aatctagaggtaccGGCCGCGTTCATGTAGTTCGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGACCTGCCG CATCTGCCAC AAgGCCTTCG CGAACGTCTA CCGCCTGCAG AGGCACATGA
0061 TCAGCCACGA CGAGAGTGCG CTGCTGCGGA AGTTTAAGTG CAAGGAGTGC GACAAGGCCT
0121 TCAAGTTCAA GCACCACCTC AAGGAGCACG TGAGGATCCA TTCCGGCGAG AAGcCCTTCG
0181 GATGCGACAA CTGCGGCAAg CGATTCTCGC ACTCGGGCAG CTTCTCCTCC CACATGACCT
0241 CCAAGAAGTG CATCAGCATG GGCCTCAAGC TGAACAACAA TCGCGCTCTg CTGAAGCGAC
0301 TGGAGAAGAG CCCGGGATCC GCATCCTCCG CATCCCGGCG ATCGCCCTCC GATCACGGAA
0361 AGGGCAAGCT GCCGGAGCAG CCGTCGCTAC CGGGACTACC ACATCCGATG AGCTACTTCG
0421 CCAGCGATGC GCAGGTGCAA GGTGGAAGTG CGGCACCCGC GCCCTTCCCG CCATTCCATC
0481 CGAACTACAT GAACGCGGCC |
| in silico PCR Fragment |
0001 AGACCTGCCG CATCTGCCAC AAGGCCTTCG CGAACGTCTA CCGCCTGCAG AGGCACATGA
0061 TCAGCCACGA CGAGAGTGCG CTGCTGCGGA AGTTTAAGTG CAAGGAGTGC GACAAGGCCT
0121 TCAAGTTCAA GCACCACCTC AAGGAGCACG TGAGGATCCA TTCCGGCGAG AAGCCCTTCG
0181 GATGCGACAA CTGCGGCAAG CGATTCTCGC ACTCGGGCAG CTTCTCCTCC CACATGACCT
0241 CCAAGAAGTG CATCAGCATG GGCCTCAAGC TGAACAACAA TCGCGCTCTG CTGAAGCGAC
0301 TGGAGAAGAG CCCGGGATCC GCATCCTCCG CATCCCGGCG ATCGCCCTCC GATCACGGAA
0361 AGGGCAAGCT GCCGGAGCAG CCGTCGCTAC CGGGACTACC ACATCCGATG AGCTACTTCG
0421 CCAGCGATGC GCAGGTGCAA GGTGGAAGTG CGGCACCCGC GCCCTTCCCG CCATTCCATC
0481 CGAACTACAT GAACGCGGCC |
| Assemble Data |
1322R-2.IR_full 1 AGACCTGCCGCATCTGCCACAAGGCCTTCGCGAACGTCTACCGCCTGCAGAGGCACATGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1322R-2.in silico 1 AGACCTGCCGCATCTGCCACAAGGCCTTCGCGAACGTCTACCGCCTGCAGAGGCACATGA 60
1322R-2.IR_full 61 TCAGCCACGACGAGAGTGCGCTGCTGCGGAAGTTTAAGTGCAAGGAGTGCGACAAGGCCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1322R-2.in silico 61 TCAGCCACGACGAGAGTGCGCTGCTGCGGAAGTTTAAGTGCAAGGAGTGCGACAAGGCCT 120
1322R-2.IR_full 121 TCAAGTTCAAGCACCACCTCAAGGAGCACGTGAGGATCCATTCCGGCGAGAAGCCCTTCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1322R-2.in silico 121 TCAAGTTCAAGCACCACCTCAAGGAGCACGTGAGGATCCATTCCGGCGAGAAGCCCTTCG 180
1322R-2.IR_full 181 GATGCGACAACTGCGGCAAGCGATTCTCGCACTCGGGCAGCTTCTCCTCCCACATGACCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1322R-2.in silico 181 GATGCGACAACTGCGGCAAGCGATTCTCGCACTCGGGCAGCTTCTCCTCCCACATGACCT 240
1322R-2.IR_full 241 CCAAGAAGTGCATCAGCATGGGCCTCAAGCTGAACAACAATCGCGCTCTGCTGAAGCGAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1322R-2.in silico 241 CCAAGAAGTGCATCAGCATGGGCCTCAAGCTGAACAACAATCGCGCTCTGCTGAAGCGAC 300
1322R-2.IR_full 301 TGGAGAAGAGCCCGGGATCCGCATCCTCCGCATCCCGGCGATCGCCCTCCGATCACGGAA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1322R-2.in silico 301 TGGAGAAGAGCCCGGGATCCGCATCCTCCGCATCCCGGCGATCGCCCTCCGATCACGGAA 360
1322R-2.IR_full 361 AGGGCAAGCTGCCGGAGCAGCCGTCGCTACCGGGACTACCACATCCGATGAGCTACTTCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1322R-2.in silico 361 AGGGCAAGCTGCCGGAGCAGCCGTCGCTACCGGGACTACCACATCCGATGAGCTACTTCG 420
1322R-2.IR_full 421 CCAGCGATGCGCAGGTGCAAGGTGGAAGTGCGGCACCCGCGCCCTTCCCGCCATTCCATC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1322R-2.in silico 421 CCAGCGATGCGCAGGTGCAAGGTGGAAGTGCGGCACCCGCGCCCTTCCCGCCATTCCATC 480
1322R-2.IR_full 481 CGAACTACATGAACGCGGCC 500
||||||||||||||||||||
1322R-2.in silico 481 CGAACTACATGAACGCGGCC 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100.2 |
483 |
12 |
20 |
23 |
NM_057502.2 |
CG1322-RB, transcript variant B (zfh1), mRNA |
| 100.2 |
483 |
12 |
20 |
23 |
NM_170523.2 |
CG1322-RA, transcript variant A (zfh1), mRNA |
| 8.71 |
42 |
13 |
21 |
24 |
NM_170522.2 |
CG1322-RC, transcript variant C (zfh1), mRNA |
| 0.2 |
1 |
4 |
2 |
5 |
NM_164996.1 |
CG31760-RA (CG31760), mRNA |
| 0.2 |
1 |
3 |
2 |
5 |
NM_001043134.1 |
CG7368-RB (CG7368), mRNA |
| 0.2 |
1 |
2 |
3 |
3 |
NM_168142.1 |
CG32415-RA (CG32415), mRNA |
| 0 |
0 |
3 |
7 |
20 |
NM_079671.2 |
CG7847-RA, transcript variant A (sr), mRNA |
| 0 |
0 |
3 |
7 |
20 |
NM_169786.1 |
CG7847-RB, transcript variant B (sr), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_168575.1 |
CG32138-RA, transcript variant A (CG32138), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_168576.1 |
CG32138-RB, transcript variant B (CG32138), mRNA |
| 0 |
0 |
2 |
9 |
15 |
NM_138017.1 |
CG3065-RA, transcript variant A (CG3065), mRNA |
| 0 |
0 |
2 |
9 |
15 |
NM_166642.1 |
CG3065-RB, transcript variant B (CG3065), mRNA |
| 0 |
0 |
2 |
9 |
15 |
NM_166643.1 |
CG3065-RC, transcript variant C (CG3065), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_135248.1 |
CG13776-RA (CG13776), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_168361.1 |
CG16711-RB, transcript variant B (CG16711), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_140090.1 |
CG16711-RA, transcript variant A (CG16711), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_138037.1 |
CG10339-RA (CG10339), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_001043243.1 |
CG6930-RB, transcript variant B (l(3)neo38), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_001043244.1 |
CG6930-RC, transcript variant C (l(3)neo38), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_136104.2 |
CG10687-RA (Aats-asn), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_141856.2 |
CG6930-RA, transcript variant A (l(3)neo38), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132313.1 |
CG15368-RA (CG15368), mRNA |
| 0 |
0 |
1 |
9 |
13 |
NM_140060.1 |
CG3445-RA (phol), mRNA |
| 0 |
0 |
1 |
7 |
23 |
NM_206275.2 |
CG12605-RA, transcript variant A (CG12605), mRNA |
| 0 |
0 |
1 |
7 |
23 |
NM_139588.3 |
CG12605-RB, transcript variant B (CG12605), mRNA |
| 0 |
0 |
1 |
7 |
22 |
NM_206274.2 |
CG12605-RC, transcript variant C (CG12605), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_169589.1 |
CG31317-RB, transcript variant B (stumps), mRNA |
| 0 |
0 |
1 |
5 |
16 |
NM_144448.2 |
CG1915-RC, transcript variant C (sls), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_079937.2 |
CG1915-RA, transcript variant A (sls), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_057569.2 |
CG4491-RA (noc), mRNA |
|
|
|
