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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13224R-2 |
| Symbol |
Cpr47Eb |
Full Name |
Cuticular protein 47Eb |
| CG No |
CG13224 |
Old CG No |
CG13224 |
| Synonyms |
Cpr47Eb, CG13224 |
| Accession No (Link to NCBI) |
NM_136811.2 |
| Inserted Chr. |
X |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.
An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.
Zoolog Sci (2016) 33(6) 583-591
[
PubMed ID = 27927092
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGCACCACCGAGAAGCGTGAG |
| Primer Seq. 3' |
aatctagaggtaccGCATCGAGGACCACCTGGACA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGCACNACCG AGAAGCGTGA GATTGTGCCT CTGCTGAGGT TTGAGACGAA CAAGAACCCC
0061 GATGGCTCCT TCCACTTCAG CTACGAGGGC GGTGACCAGT CCGTGCGCCA GGAGCAGGGA
0121 GTGATCGAGA ACGCCGGCAC CGAGGACGAG GCCTTGGAGG TGTCCGGTAT GTACAGCTAC
0181 ATCGACGCCG ATGGCAACAC CGTGGAAGTG CACTACACCG CCGGAAAGAA CGGATNTCGT
0241 GCCCATTGGC ACCATCATTC CCAAGGAGAT CACCGAGTTG GCCAAGTCAG CTGCCTTTCT
0301 GCCCAAGGTT TCCGAGGATG AGCAGAAGTA TCGCAANGGC CCGCTCCCAG GAACTGGACA
0361 ATAAGGAAGT TGCTGTAGAG AAGGAAGCCG CCCCAGTGGA GAAGGAGGAG CCTGTGGTTG
0421 CCAAGGAATC GGTTGTGGTG GAGAAATCCG AGCCCCTGCC CGCCGAGTCC CAGGTGGCGC
0481 CTGTCCAGGT GGTCCTCGAT GC |
| in silico PCR Fragment |
0001 AGCACCACCG AGAAGCGTGA GATTGTGCCT CTGCTGAGGT TTGAGACGAA CAAGAACCCC
0061 GATGGCTCCT TCCACTTCAG CTACGAGGGC GGTGACCAGT CCGTGCGCCA GGAGCAGGGA
0121 GTGATCGAGA ACGCCGGCAC CGAGGACGAG GCCTTGGAGG TGTCCGGTAT GTACAGCTAC
0181 ATCGACGCCG ATGGCAACAC CGTGGAGGTG CACTACACCG CCGGAAAGAA CGGAT-TCGT
0241 GCCCATTGGC ACCATCATTC CCAAGGAGAT CACCGAGTTG GCCAAGTCAG CTGCCCTTCT
0301 GCCCAAGGTT TCCGAGGATG AGCAGAAGTA TCGCAA-GGC CCGCTCCCAG GAACTGGACA
0361 ATAAGGAAGT TGCTGTAGAG AAGGAAGCCG CCCCAGTGGA GAAGGAGGAG CCTGTGGTTG
0421 CCAAGGAATC GGTTGTGGTG GAGAAATCCG AGCCCCTGCC CGCCGAGTCC CAGGTGGCGC
0481 CTGTCCAGGT GGTCCTCGAT GC |
| Assemble Data |
13224R-2.IR_full 1 AGCACNACCGAGAAGCGTGAGATTGTGCCTCTGCTGAGGTTTGAGACGAACAAGAACCCC 60
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
13224R-2.in silico 1 AGCACCACCGAGAAGCGTGAGATTGTGCCTCTGCTGAGGTTTGAGACGAACAAGAACCCC 60
13224R-2.IR_full 61 GATGGCTCCTTCCACTTCAGCTACGAGGGCGGTGACCAGTCCGTGCGCCAGGAGCAGGGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13224R-2.in silico 61 GATGGCTCCTTCCACTTCAGCTACGAGGGCGGTGACCAGTCCGTGCGCCAGGAGCAGGGA 120
13224R-2.IR_full 121 GTGATCGAGAACGCCGGCACCGAGGACGAGGCCTTGGAGGTGTCCGGTATGTACAGCTAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13224R-2.in silico 121 GTGATCGAGAACGCCGGCACCGAGGACGAGGCCTTGGAGGTGTCCGGTATGTACAGCTAC 180
13224R-2.IR_full 181 ATCGACGCCGATGGCAACACCGTGGAAGTGCACTACACCGCCGGAAAGAACGGATNTCGT 240
|||||||||||||||||||||||||| |||||||||||||||||||||||||||| ||||
13224R-2.in silico 181 ATCGACGCCGATGGCAACACCGTGGAGGTGCACTACACCGCCGGAAAGAACGGAT-TCGT 240
13224R-2.IR_full 241 GCCCATTGGCACCATCATTCCCAAGGAGATCACCGAGTTGGCCAAGTCAGCTGCCTTTCT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
13224R-2.in silico 241 GCCCATTGGCACCATCATTCCCAAGGAGATCACCGAGTTGGCCAAGTCAGCTGCCCTTCT 300
13224R-2.IR_full 301 GCCCAAGGTTTCCGAGGATGAGCAGAAGTATCGCAANGGCCCGCTCCCAGGAACTGGACA 360
|||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
13224R-2.in silico 301 GCCCAAGGTTTCCGAGGATGAGCAGAAGTATCGCAA-GGCCCGCTCCCAGGAACTGGACA 360
13224R-2.IR_full 361 ATAAGGAAGTTGCTGTAGAGAAGGAAGCCGCCCCAGTGGAGAAGGAGGAGCCTGTGGTTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13224R-2.in silico 361 ATAAGGAAGTTGCTGTAGAGAAGGAAGCCGCCCCAGTGGAGAAGGAGGAGCCTGTGGTTG 420
13224R-2.IR_full 421 CCAAGGAATCGGTTGTGGTGGAGAAATCCGAGCCCCTGCCCGCCGAGTCCCAGGTGGCGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13224R-2.in silico 421 CCAAGGAATCGGTTGTGGTGGAGAAATCCGAGCCCCTGCCCGCCGAGTCCCAGGTGGCGC 480
13224R-2.IR_full 481 CTGTCCAGGTGGTCCTCGATGC 502
||||||||||||||||||||||
13224R-2.in silico 481 CTGTCCAGGTGGTCCTCGATGC 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_136811.2 |
CG13224-RA (CG13224), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_168268.1 |
CG7176-RE, transcript variant E (Idh), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_168266.1 |
CG7176-RD, transcript variant D (Idh), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_143787.2 |
CG7176-RC, transcript variant C (Idh), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_168265.1 |
CG7176-RB, transcript variant B (Idh), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_176298.1 |
CG7176-RG, transcript variant G (Idh), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_168267.1 |
CG7176-RA, transcript variant A (Idh), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_168269.1 |
CG7176-RF, transcript variant F (Idh), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_135226.2 |
CG11221-RA (CG11221), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_143061.1 |
CG13648-RA (tnc), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_135689.2 |
CG12264-RA (CG12264), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_079449.2 |
CG7978-RA (Ac76E), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_078589.2 |
CG1903-RB, transcript variant B (sno), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_136571.2 |
CG8262-RA (CG8262), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_078517.2 |
CG2175-RA, transcript variant A (dec-1), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167590.1 |
CG32495-RA, transcript variant A (CG32495), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167589.1 |
CG32495-RB, transcript variant B (CG32495), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167591.1 |
CG32495-RC, transcript variant C (CG32495), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_176752.1 |
CG6835-RC, transcript variant C (GS), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_176753.1 |
CG6835-RD, transcript variant D (GS), mRNA |
| 0 |
0 |
1 |
8 |
2 |
NM_142638.2 |
CG5412-RA (CG5412), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_137964.1 |
CG5372-RA (alphaPS5), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_140064.2 |
CG32043-RA, transcript variant A (CG32043), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_168346.1 |
CG32043-RB, transcript variant B (CG32043), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_130621.2 |
CG4061-RA (CG4061), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136399.2 |
CG9447-RA (CG9447), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_166924.1 |
CG4199-RF, transcript variant F (CG4199), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137394.1 |
CG11423-RA (CG11423), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001032249.1 |
CG30084-RF, transcript variant F (CG30084), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_136044.2 |
CG10333-RA (CG10333), mRNA |
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