NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

13210R-2

Symbol

CG13210

Full Name

CG13210

CG No

CG13210

Old CG No

CG13210

Synonyms

CG13210

Accession No (Link to NCBI)

NM_136823.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGCTGAGGATCCGGTGAAC

Primer Seq. 3'

aatctagaggtaccTCGCCCTTTAAGCAGAAACCC

Predicted Fragment Size

IR fragment full Seq

0001 NTGGCTGAGG ATCCGGTGAA CCAGCAGGCA TTCGCTGAGG ACGTGGCAGC TGcCGCTGCG 
0061 GCTGGAGGAG CCGGCGGTTT TGCCGGTCCC GAGCCCGGAC ATCCCGACTT TAAGATGCCC 
0121 AGTGCCGAGG AGATCTGGAA GCTGGTCGAG GGCATGGAGG GAATTTCGGA CGACGAGCGA 
0181 GCCGAGCTCC GCGAGAGCAT CTTTAACCCG AAGCCGCCGT CGCCGGAGGA CTTCATGCGC 
0241 CAGTACGGGC ATCCGGGCCA CAGTTCCCGG GAGTACCTGG TGTTCTTCGT GATGCTGGCC 
0301 CTCATACTCA TTGTCTTTGG TAAGCGTCCT CCGGAACGAG CCACTAACGA GTCGTATAAC 
0361 CCTTATCGGG CGCGATATAC GCACCCACCT GGGTTTCTGC TTAAAGGGCG A

in silico PCR Fragment

0001 ATGGCTGAGG ATCCGGTGAA CCAGCAGGCA TTCGCTGAGG ACGTGGCAGC TGCCGCTGCG 
0061 GCTGGAGGAG CCGGCGGTTT TGCCGGTCCC GAGCCCGGAC ATCCCGACTT TAAGATGCCC 
0121 AGTGCCGAGG AGATCTGGAA GCTGGTCGAG GGCATGGAGG GAATTTCGGA CGACGAGCGA 
0181 GCCGAGCTCC GCGAGAGCAT CTTTAACCCG AAGCCGCCGT CGCCGGAGGA CTTCATGCGC 
0241 CAGTACGGGC ATCCGGGCCA CAGTTCCCGG GAGTACCTGG TGTTCTTCGT GATGCTGGCC 
0301 CTCATACTCA TTGTCTTTGG TAAGCGTCCT CCGGAACGAG CCACTAACGA GTCGTATAAC 
0361 CCTTATCGGG CGCGATATAC GCACCCACCT GGGTTTCTGC TTAAAGGGCG A

Assemble Data


13210R-2.IR_full        1   TGGCTGAGGATCCGGTGAACCAGCAGGCATTCGCTGAGGACGTGGCAGCTGCCGCTGCG 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13210R-2.in silico      1   TGGCTGAGGATCCGGTGAACCAGCAGGCATTCGCTGAGGACGTGGCAGCTGCCGCTGCG 59


13210R-2.IR_full       61  GCTGGAGGAGCCGGCGGTTTTGCCGGTCCCGAGCCCGGACATCCCGACTTTAAGATGCCC 119
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13210R-2.in silico     61  GCTGGAGGAGCCGGCGGTTTTGCCGGTCCCGAGCCCGGACATCCCGACTTTAAGATGCCC 119


13210R-2.IR_full       121 AGTGCCGAGGAGATCTGGAAGCTGGTCGAGGGCATGGAGGGAATTTCGGACGACGAGCGA 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13210R-2.in silico     121 AGTGCCGAGGAGATCTGGAAGCTGGTCGAGGGCATGGAGGGAATTTCGGACGACGAGCGA 179


13210R-2.IR_full       181 GCCGAGCTCCGCGAGAGCATCTTTAACCCGAAGCCGCCGTCGCCGGAGGACTTCATGCGC 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13210R-2.in silico     181 GCCGAGCTCCGCGAGAGCATCTTTAACCCGAAGCCGCCGTCGCCGGAGGACTTCATGCGC 239


13210R-2.IR_full       241 CAGTACGGGCATCCGGGCCACAGTTCCCGGGAGTACCTGGTGTTCTTCGTGATGCTGGCC 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13210R-2.in silico     241 CAGTACGGGCATCCGGGCCACAGTTCCCGGGAGTACCTGGTGTTCTTCGTGATGCTGGCC 299


13210R-2.IR_full       301 CTCATACTCATTGTCTTTGGTAAGCGTCCTCCGGAACGAGCCACTAACGAGTCGTATAAC 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13210R-2.in silico     301 CTCATACTCATTGTCTTTGGTAAGCGTCCTCCGGAACGAGCCACTAACGAGTCGTATAAC 359


13210R-2.IR_full       361 CCTTATCGGGCGCGATATACGCACCCACCTGGGTTTCTGCTTAAAGGGCGA 410
                           |||||||||||||||||||||||||||||||||||||||||||||||||||
13210R-2.in silico     361 CCTTATCGGGCGCGATATACGCACCCACCTGGGTTTCTGCTTAAAGGGCGA 410

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	393	0	0	0	NM_136823.1	CG13210-RA (CG13210), mRNA
0.5	2	6	2	2	NM_169103.1	CG10978-RB, transcript variant B (jagn), mRNA
0.5	2	6	2	2	NM_141328.1	CG10978-RA, transcript variant A (jagn), mRNA
0.5	2	3	3	5	NM_165425.2	CG2682-RB, transcript variant B (d4), mRNA
0.5	2	3	3	5	NM_136319.3	CG2682-RA, transcript variant A (d4), mRNA
0.5	2	3	3	5	NM_165424.2	CG2682-RC, transcript variant C (d4), mRNA
0	0	3	8	15	NM_206407.1	CG32434-RA, transcript variant A (siz), mRNA
0	0	3	8	15	NM_001043153.1	CG32434-RC, transcript variant C (siz), mRNA
0	0	3	8	15	NM_168881.1	CG32434-RB, transcript variant B (siz), mRNA
0	0	3	2	5	NM_170179.1	CG31121-RA, transcript variant A (CG31121), mRNA
0	0	3	2	4	NM_167557.2	CG8923-RB, transcript variant B (mei-218), mRNA
0	0	3	2	4	NM_001031897.1	CG8923-RC, transcript variant C (mei-218), mRNA
0	0	3	2	4	NM_001031898.1	CG33935-RA, transcript variant A (mei-217), mRNA
0	0	2	3	5	NM_001043243.1	CG6930-RB, transcript variant B (l(3)neo38), mRNA
0	0	2	3	5	NM_001043244.1	CG6930-RC, transcript variant C (l(3)neo38), mRNA
0	0	2	3	5	NM_079703.2	CG3593-RA (r-l), mRNA
0	0	2	2	22	NM_132246.2	CG10555-RA (CG10555), mRNA
0	0	2	2	3	NM_080331.2	CG3578-RA (bi), mRNA
0	0	1	7	3	NM_078908.2	CG12845-RA (Tsp42Ef), mRNA
0	0	1	5	4	NM_144209.1	CG12626-RA (CG12626), mRNA
0	0	1	5	3	NM_135093.2	CG14015-RA (CG14015), mRNA
0	0	1	4	6	NM_140200.1	CG6175-RB (CG6175), mRNA
0	0	1	4	2	NM_140052.1	CG3967-RA, transcript variant A (CG3967), mRNA
0	0	1	4	2	NM_170631.1	CG3967-RE, transcript variant E (CG3967), mRNA
0	0	1	4	2	NM_170630.1	CG3967-RD, transcript variant D (CG3967), mRNA
0	0	1	4	2	NM_140053.2	CG3967-RC, transcript variant C (CG3967), mRNA
0	0	1	3	25	NM_132413.1	CG15295-RA (CG15295), mRNA
0	0	1	3	8	NM_142034.1	CG9764-RA (yrt), mRNA
0	0	1	3	7	NM_136601.1	CG8197-RA (CG8197), mRNA
0	0	1	3	5	NM_176685.1	CG33080-RA, transcript variant A (CG33080), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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