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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13208R-2 
 Symbol Obp47b  Full Name Odorant-binding protein 47b 
 CG No CG13208  Old CG No CG13208 
 Synonyms CG13208, Obp47b 
 Accession No (Link to NCBI) NM_136825.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92 [ PubMed ID = 19363474 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTCGCTGGCCCTAAATACTCGT 
 Primer Seq. 3' 
aatctagaggtaccCGATGATCGGGGCAGTTCTTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TCGCTGGCCC TAAATACTCG TCTAGTATTT GGCCAAGCCA CGATTGATTG CCAAAGACCA 
0061 CCGCAACTAG TGGATCCCGC ACTCTGCTGC AAGGATGGGG GTCGCGACCA GGTGGCTGAG 
0121 CAGTGCGCCC AACGGATTTT AGGAACAGCA AATGGACAGA AGGCAGGAGG TCCTCCATCT 
0181 TTGGACACGG CAGCATGCCT AGCTGAGTGC ATCCTCACAT CCTCCAAGTA TATTGATGAG 
0241 CCCCAGAAAC TAAACCTGGC AAACATACGC AGTGATCTCT CCGCTAAGTT CTCCAACGAT 
0301 ACCCTTTACG TGGAAACCAT GACCATGGCG TTCTCCAAGT GTGAGCCCCA ATCGCAGCGC 
0361 AGACTGGCCA TGATCATGCA GCAGCAACAA CAGGTGCAGC AGCAAAAGAC GCAGCAGCAA 
0421 CAACCAAGAT GCAGCCCCTT CTCAGCCATC GTCCTTGGGT GCACCTACAT GGAGTACTTC 
0481 AAGAACTGCC CCGATCATCG  
 in silico PCR Fragment 
0001 TCGCTGGCCC TAAATACTCG TCTAGTATTT GGCCAAGCCA CGATTGATTG CCAAAGACCA 
0061 CCGCAACTAG TGGATCCCGC ACTCTGCTGC AAGGATGGGG GTCGCGACCA GGTGGCTGAG 
0121 CAGTGCGCCC AACGGATTTT AGGAACAGCA AATGGACAGA AGGCAGGAGG TCCTCCATCT 
0181 TTGGACACGG CAGCATGCCT AGCTGAGTGC ATCCTCACAT CCTCCAAGTA TATTGATGAG 
0241 CCCCAGAAAC TAAACCTGGC AAACATACGC AGTGATCTCT CCGCTAAGTT CTCCAACGAT 
0301 ACCCTTTACG TGGAAACCAT GACCATGGCG TTCTCCAAGT GTGAGCCCCA ATCGCAGCGC 
0361 AGACTGGCCA TGATCATGCA GCAGCAACAA CAGGTGCAGC AGCAAAAGAC GCAGCAGCAA 
0421 CAACCAAGAT GCAGCCCCTT CTCAGCCATC GTCCTTGGGT GCACCTACAT GGAGTACTTC 
0481 AAGAACTGCC CCGATCATCG  
 Assemble Data 

13208R-2.IR_full       1   TCGCTGGCCCTAAATACTCGTCTAGTATTTGGCCAAGCCACGATTGATTGCCAAAGACCA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13208R-2.in silico     1   TCGCTGGCCCTAAATACTCGTCTAGTATTTGGCCAAGCCACGATTGATTGCCAAAGACCA 60


13208R-2.IR_full       61  CCGCAACTAGTGGATCCCGCACTCTGCTGCAAGGATGGGGGTCGCGACCAGGTGGCTGAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13208R-2.in silico     61  CCGCAACTAGTGGATCCCGCACTCTGCTGCAAGGATGGGGGTCGCGACCAGGTGGCTGAG 120


13208R-2.IR_full       121 CAGTGCGCCCAACGGATTTTAGGAACAGCAAATGGACAGAAGGCAGGAGGTCCTCCATCT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13208R-2.in silico     121 CAGTGCGCCCAACGGATTTTAGGAACAGCAAATGGACAGAAGGCAGGAGGTCCTCCATCT 180


13208R-2.IR_full       181 TTGGACACGGCAGCATGCCTAGCTGAGTGCATCCTCACATCCTCCAAGTATATTGATGAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13208R-2.in silico     181 TTGGACACGGCAGCATGCCTAGCTGAGTGCATCCTCACATCCTCCAAGTATATTGATGAG 240


13208R-2.IR_full       241 CCCCAGAAACTAAACCTGGCAAACATACGCAGTGATCTCTCCGCTAAGTTCTCCAACGAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13208R-2.in silico     241 CCCCAGAAACTAAACCTGGCAAACATACGCAGTGATCTCTCCGCTAAGTTCTCCAACGAT 300


13208R-2.IR_full       301 ACCCTTTACGTGGAAACCATGACCATGGCGTTCTCCAAGTGTGAGCCCCAATCGCAGCGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13208R-2.in silico     301 ACCCTTTACGTGGAAACCATGACCATGGCGTTCTCCAAGTGTGAGCCCCAATCGCAGCGC 360


13208R-2.IR_full       361 AGACTGGCCATGATCATGCAGCAGCAACAACAGGTGCAGCAGCAAAAGACGCAGCAGCAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13208R-2.in silico     361 AGACTGGCCATGATCATGCAGCAGCAACAACAGGTGCAGCAGCAAAAGACGCAGCAGCAA 420


13208R-2.IR_full       421 CAACCAAGATGCAGCCCCTTCTCAGCCATCGTCCTTGGGTGCACCTACATGGAGTACTTC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13208R-2.in silico     421 CAACCAAGATGCAGCCCCTTCTCAGCCATCGTCCTTGGGTGCACCTACATGGAGTACTTC 480


13208R-2.IR_full       481 AAGAACTGCCCCGATCATCG 500
                           ||||||||||||||||||||
13208R-2.in silico     481 AAGAACTGCCCCGATCATCG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  3  NM_136825.1  CG13208-RA (Obp47b), mRNA 
92.94   448  4  4  7  NM_136826.1  CG7741-RA (CG7741), mRNA 
0.2   1  3  6  41  NM_057422.2  CG8651-RB, transcript variant B (trx), mRNA 
0.2   1  3  6  41  NM_134282.1  CG8651-RA, transcript variant A (trx), mRNA 
0.2   1  3  6  39  NM_134281.1  CG8651-RC, transcript variant C (trx), mRNA 
0.2   1  3  6  39  NM_057421.2  CG8651-RD, transcript variant D (trx), mRNA 
0.2   1  3  6  39  NM_001014621.1  CG8651-RE, transcript variant E (trx), mRNA 
0   0  18  96  197  NM_132525.1  CG15740-RA (CG15740), mRNA 
0   0  10  46  92  NM_168179.1  CG32394-RA (CG32394), mRNA 
0   0  10  11  103  NM_166873.1  CG3638-RB, transcript variant B (CG3638), mRNA 
0   0  10  11  103  NM_166872.1  CG3638-RD, transcript variant D (CG3638), mRNA 
0   0  10  11  103  NM_130541.3  CG3638-RC, transcript variant C (CG3638), mRNA 
0   0  10  11  103  NM_166874.1  CG3638-RA, transcript variant A (CG3638), mRNA 
0   0  10  4  55  NM_170524.1  CG12072-RA (wts), mRNA 
0   0  9  6  46  NM_137891.1  CG3695-RA (MED23), mRNA 
0   0  7  2  4  NM_001042809.1  CG15720-RB, transcript variant B (CG15720), mRNA 
0   0  7  2  4  NM_132620.1  CG15720-RA, transcript variant A (CG15720), mRNA 
0   0  6  17  70  NM_001014500.1  CG33558-RA (CG33558), mRNA 
0   0  6  14  26  NM_001043153.1  CG32434-RC, transcript variant C (siz), mRNA 
0   0  6  14  26  NM_206407.1  CG32434-RA, transcript variant A (siz), mRNA 
0   0  6  14  26  NM_168881.1  CG32434-RB, transcript variant B (siz), mRNA 
0   0  6  10  41  NM_169025.1  CG2534-RB, transcript variant B (cno), mRNA 
0   0  6  10  41  NM_079508.2  CG2534-RA, transcript variant A (cno), mRNA 
0   0  6  9  57  NM_167595.3  CG12348-RE, transcript variant E (Sh), mRNA 
0   0  6  8  40  NM_167594.2  CG12348-RC, transcript variant C (Sh), mRNA 
0   0  6  2  2  NM_136723.1  CG12913-RA (CG12913), mRNA 
0   0  5  15  103  NM_135785.1  CG6043-RD, transcript variant D (CG6043), mRNA 
0   0  5  15  103  NM_165035.1  CG6043-RB, transcript variant B (CG6043), mRNA 
0   0  5  15  103  NM_165037.1  CG6043-RC, transcript variant C (CG6043), mRNA 
0   0  5  15  103  NM_165034.1  CG6043-RA, transcript variant A (CG6043), mRNA 

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