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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13189R-3 
 Symbol CG13189  Full Name CG13189 
 CG No CG13189  Old CG No CG13189 
 Synonyms CG13189 
 Accession No (Link to NCBI) NM_136868.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGCCGCCCTAGTGATCTTCGT 
 Primer Seq. 3' 
aatctagaggtaccTCTTCCACTGGGACAGGGCGT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGCCGCCCTA GTGATCTTCG TCCGGGGTAA CCAGCGGAGG TCTCTGGACG CCGCCCTGGG 
0061 ATTCCGCAGC TGGCGTAATG ATAGCAGCTC TCCTTTTGGT CACTCCTCAA GCCGGCCATC 
0121 GAAATGGCCG AGAGTTCCCA TTTGTACGGA GTATACGCCT TTCTGCCCGT CGCTGGAGGC 
0181 TTCCTCTTGG GATCGATATT TGTCTACGGA TGCGATAAAT TGATGTCATA CCTGGGCCTT 
0241 AACAGCACCA ACATGATGAT CCAGATGACG CAATCGAAAG CCAAGGCGGA CATAGCCATC 
0301 GAGGACTCTA AGCGCAATGG AGTTGCCCCC GATAGGTTGG CCTCAAAGAG TCTGGACAGC 
0361 TTCTCCGACT GCCTTAGCGT GCAGCACAGC GGCGAATCGA GACGCCGAAA GAAGGGAGCT 
0421 AGCATCAACG AGATGGAGCA GTGCACCTAC ACCACGCCTG AGGAGCAGCA GCGCGCCGAG 
0481 GACGCCCTGT CCCAGTGGAA GA 
 in silico PCR Fragment 
0001 CGCCGCCCTA GTGATCTTCG TCCGGGGTAA CCAGCGGAGG TCTCTGGACG CCGCCCTGGG 
0061 ATT-CGCAGC TGGCGTAATG ATAGCAGC-C TCCTTTTGGT CACTCCTCAA GCCGGCCATC 
0121 GAAATGGCCG AGAGTTCCCA TTTGTACGGA GTATACGCCT TTCTGCCCGT CGCTGGAGGC 
0181 TTCCTCTTGG GATCGATCTT CGTCTACGGA TGCGACAAAT TGATGTCATA CCTGGGCCTT 
0241 AACAGCACCA ACATGATGAT CCAGATGACG CAATCAAAAG CCAAGGCGGA CATAGCCATC 
0301 GAGGACTCTA AGCGCAATGG AGTTGCCCCC GATAGGTTGG CCTCAAAGAG TCTGGACAGC 
0361 TTCTCCGACT GCCTTAGCGT GCAGCACAGC GGCGAATCGA GACGCCGAAA GAAGGGAGCT 
0421 AGCATCAACG AGATGGAGCA GTGCACCTAC ACCACGCCTG AGGAGCAGCA GCGCGCCGAG 
0481 GACGCCCTGT CCCAGTGGAA GA 
 Assemble Data 

13189R-3.IR_full       1   CGCCGCCCTAGTGATCTTCGTCCGGGGTAACCAGCGGAGGTCTCTGGACGCCGCCCTGGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13189R-3.in silico     1   CGCCGCCCTAGTGATCTTCGTCCGGGGTAACCAGCGGAGGTCTCTGGACGCCGCCCTGGG 60


13189R-3.IR_full       61  ATTCCGCAGCTGGCGTAATGATAGCAGCTCTCCTTTTGGTCACTCCTCAAGCCGGCCATC 120
                           ||| |||||||||||||||||||||||| |||||||||||||||||||||||||||||||
13189R-3.in silico     61  ATT-CGCAGCTGGCGTAATGATAGCAGC-CTCCTTTTGGTCACTCCTCAAGCCGGCCATC 120


13189R-3.IR_full       121 GAAATGGCCGAGAGTTCCCATTTGTACGGAGTATACGCCTTTCTGCCCGTCGCTGGAGGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13189R-3.in silico     121 GAAATGGCCGAGAGTTCCCATTTGTACGGAGTATACGCCTTTCTGCCCGTCGCTGGAGGC 180


13189R-3.IR_full       181 TTCCTCTTGGGATCGATATTTGTCTACGGATGCGATAAATTGATGTCATACCTGGGCCTT 240
                           ||||||||||||||||| || |||||||||||||| ||||||||||||||||||||||||
13189R-3.in silico     181 TTCCTCTTGGGATCGATCTTCGTCTACGGATGCGACAAATTGATGTCATACCTGGGCCTT 240


13189R-3.IR_full       241 AACAGCACCAACATGATGATCCAGATGACGCAATCGAAAGCCAAGGCGGACATAGCCATC 300
                           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
13189R-3.in silico     241 AACAGCACCAACATGATGATCCAGATGACGCAATCAAAAGCCAAGGCGGACATAGCCATC 300


13189R-3.IR_full       301 GAGGACTCTAAGCGCAATGGAGTTGCCCCCGATAGGTTGGCCTCAAAGAGTCTGGACAGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13189R-3.in silico     301 GAGGACTCTAAGCGCAATGGAGTTGCCCCCGATAGGTTGGCCTCAAAGAGTCTGGACAGC 360


13189R-3.IR_full       361 TTCTCCGACTGCCTTAGCGTGCAGCACAGCGGCGAATCGAGACGCCGAAAGAAGGGAGCT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13189R-3.in silico     361 TTCTCCGACTGCCTTAGCGTGCAGCACAGCGGCGAATCGAGACGCCGAAAGAAGGGAGCT 420


13189R-3.IR_full       421 AGCATCAACGAGATGGAGCAGTGCACCTACACCACGCCTGAGGAGCAGCAGCGCGCCGAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13189R-3.in silico     421 AGCATCAACGAGATGGAGCAGTGCACCTACACCACGCCTGAGGAGCAGCAGCGCGCCGAG 480


13189R-3.IR_full       481 GACGCCCTGTCCCAGTGGAAGA 502
                           ||||||||||||||||||||||
13189R-3.in silico     481 GACGCCCTGTCCCAGTGGAAGA 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_136868.1  CG13189-RA (CG13189), mRNA 
0   0  5  2  4  NM_132213.1  CG2260-RA (CG2260), mRNA 
0   0  4  2  4  NM_205894.1  CG3057-RD, transcript variant D (colt), mRNA 
0   0  4  2  4  NM_205895.1  CG3057-RC, transcript variant C (colt), mRNA 
0   0  4  2  4  NM_205896.1  CG3057-RB, transcript variant B (colt), mRNA 
0   0  4  2  4  NM_057873.4  CG3057-RA, transcript variant A (colt), mRNA 
0   0  3  2  3  NM_142123.1  CG3505-RA (CG3505), mRNA 
0   0  2  3  4  NM_130609.2  CG3587-RA (CG3587), mRNA 
0   0  2  2  6  NM_079637.4  CG4843-RA, transcript variant A (Tm2), mRNA 
0   0  2  2  6  NM_169645.2  CG4843-RC, transcript variant C (Tm2), mRNA 
0   0  2  2  6  NM_169644.1  CG4843-RB, transcript variant B (Tm2), mRNA 
0   0  1  2  5  NM_205878.1  CG11148-RC, transcript variant C (CG11148), mRNA 
0   0  1  2  5  NM_143693.2  CG11148-RA, transcript variant A (CG11148), mRNA 
0   0  1  2  5  NM_205879.1  CG11148-RB, transcript variant B (CG11148), mRNA 
0   0  1  2  3  NM_132794.2  CG18210-RA (CG18210), mRNA 
0   0  1  2  3  NM_134893.2  CG17223-RA (alpha4GT1), mRNA 
0   0  1  2  3  NM_164797.1  CG8475-RB, transcript variant B (CG8475), mRNA 
0   0  1  2  2  NM_135345.2  CG8475-RA, transcript variant A (CG8475), mRNA 
0   0  0  8  6  NM_142504.2  CG17836-RB, transcript variant B (CG17836), mRNA 
0   0  0  8  6  NM_169839.1  CG17836-RA, transcript variant A (CG17836), mRNA 
0   0  0  8  6  NM_169840.1  CG17836-RC, transcript variant C (CG17836), mRNA 
0   0  0  8  6  NM_169841.1  CG17836-RD, transcript variant D (CG17836), mRNA 
0   0  0  7  3  NM_143637.3  CG1937-RA (sip3), mRNA 
0   0  0  5  10  NM_078689.2  CG14228-RA (Mer), mRNA 
0   0  0  5  3  NM_141102.2  CG14572-RA (CG14572), mRNA 
0   0  0  4  12  NM_135755.1  CG9932-RA (CG9932), mRNA 
0   0  0  4  10  NM_143284.2  CG6059-RA (CG6059), mRNA 
0   0  0  4  9  NM_143729.2  CG17342-RA, transcript variant A (Lk6), mRNA 
0   0  0  4  7  NM_132186.1  CG10932-RA (CG10932), mRNA 
0   0  0  4  7  NM_169423.2  CG17342-RB, transcript variant B (Lk6), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
++ 
DB
++ 
DC
 
DM
+ 
VM
+- 
VB
 
Caspase-3 signal
N
++ 
DB
++ 
DC
 
DM
++ 
VM
+ 
VB
+ 
Dcompartment   Penetrance
+     

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