NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 1311R-1

Symbol CG1311 Full Name CG1311

CG No CG1311 Old CG No CG1311

Synonyms CG1311

Accession No (Link to NCBI) NM_139627.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGGCTGTGCGGAGAGCAAG

Primer Seq. 3'

aatctagaggtaccGGCGAACTCTCATGAACGGGA

Predicted Fragment Size

IR fragment full Seq

0001 ATGGGCTGTG CTGGAGAGNA AGGACGGCGA GGGGGAGGCG CAAAACAATA GGCCCAAATA 
0061 CCGGTCCTGC ACGGACACCT GCTGGCTGGC CATTTACATT ATATTCTGGC TGTTTTTGAT 
0121 AGTGATTGCC ATCTTTTCGT TCGTTTATGG AAATCCCCTG CGCATCATCA ATGGCTACGA 
0181 TTCCTTTGGC AACACCTGTG GCGTTAAGTA CAATGAAAAG TTCCAGGGAT TCCCGCTGTC 
0241 CGGCATGAAT ACACTCGACA AAGCCGGAGC TCTTCTATTT CGATGTCAAG GAGCTGAAAA 
0301 AGTCCCTCAA GATATGCGTT AAGTCATGTC CCGCCAAAAC TATGACCAAG GGCAGCGAGC 
0361 TGCTGGAATA CTACAGCCAA ACGGGCACAC ANNTCTGCAA ATACGACTAC AACATGCAGC 
0421 AGCTGACTAC GGCGGGCAAC GATGCCAAAA CGTTCAATTT cCNGGGCCCA TGTCCCAGTT 
0481 TTCCCGTTCA TGAGAGTTCN CC

in silico PCR Fragment

0001 ATGGGCTGTG C-GGAGAGCA AGGACGGCGA GGGGGAGGCG CAAAACAATA GGCCCAAATA 
0061 CCGGTCCTGC ACGGACACCT GCTGGCTGGC CATTTACATT ATATTCTGGC TGTTTTTGAT 
0121 AGTGATTGCC ATCTTTTCGT TCGTTTATGG AAATCCCCTG CGCATCATCA ATGGCTACGA 
0181 TTCCTTTGGC AACACCTGTG GCGTTAAGTA CAATGAAAAG TTCCAGGGAT TCCCGCTGTC 
0241 CGGCATGAAT ACACTCGAC- AAGCCGGAGC TCTTCTATTT CGATGTCAAG GAGCTGAAAA 
0301 AGTCCCTCAA GATATGCGTT AAGTCATGTC CCGCCAAAAC TATGACCAAG GGCAGCGAGC 
0361 TGCTGGAATA CTACAGCCAA ACGGGCACAC AGCTCTGCAA ATACGACTAC AACATGCAGC 
0421 AGCTGACTAC GGCGGGCAAC GATGCCAAAA CGTTCAATTT CCTGGGCCCA TGTCCCAGTT 
0481 TTCCCGTTCA TGAGAGTTCG CC

Assemble Data


1311R-1.IR_full       1   ATGGGCTGTGCTGGAGAGNAAGGACGGCGAGGGGGAGGCGCAAAACAATAGGCCCAAATA 60
                          ||||||||||| |||||| |||||||||||||||||||||||||||||||||||||||||
1311R-1.in silico     1   ATGGGCTGTGC-GGAGAGCAAGGACGGCGAGGGGGAGGCGCAAAACAATAGGCCCAAATA 60


1311R-1.IR_full       61  CCGGTCCTGCACGGACACCTGCTGGCTGGCCATTTACATTATATTCTGGCTGTTTTTGAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1311R-1.in silico     61  CCGGTCCTGCACGGACACCTGCTGGCTGGCCATTTACATTATATTCTGGCTGTTTTTGAT 120


1311R-1.IR_full       121 AGTGATTGCCATCTTTTCGTTCGTTTATGGAAATCCCCTGCGCATCATCAATGGCTACGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1311R-1.in silico     121 AGTGATTGCCATCTTTTCGTTCGTTTATGGAAATCCCCTGCGCATCATCAATGGCTACGA 180


1311R-1.IR_full       181 TTCCTTTGGCAACACCTGTGGCGTTAAGTACAATGAAAAGTTCCAGGGATTCCCGCTGTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1311R-1.in silico     181 TTCCTTTGGCAACACCTGTGGCGTTAAGTACAATGAAAAGTTCCAGGGATTCCCGCTGTC 240


1311R-1.IR_full       241 CGGCATGAATACACTCGACAAAGCCGGAGCTCTTCTATTTCGATGTCAAGGAGCTGAAAA 300
                          ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
1311R-1.in silico     241 CGGCATGAATACACTCGAC-AAGCCGGAGCTCTTCTATTTCGATGTCAAGGAGCTGAAAA 300


1311R-1.IR_full       301 AGTCCCTCAAGATATGCGTTAAGTCATGTCCCGCCAAAACTATGACCAAGGGCAGCGAGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1311R-1.in silico     301 AGTCCCTCAAGATATGCGTTAAGTCATGTCCCGCCAAAACTATGACCAAGGGCAGCGAGC 360


1311R-1.IR_full       361 TGCTGGAATACTACAGCCAAACGGGCACACANNTCTGCAAATACGACTACAACATGCAGC 420
                          |||||||||||||||||||||||||||||||  |||||||||||||||||||||||||||
1311R-1.in silico     361 TGCTGGAATACTACAGCCAAACGGGCACACAGCTCTGCAAATACGACTACAACATGCAGC 420


1311R-1.IR_full       421 AGCTGACTACGGCGGGCAACGATGCCAAAACGTTCAATTTCCNGGGCCCATGTCCCAGTT 480
                          |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
1311R-1.in silico     421 AGCTGACTACGGCGGGCAACGATGCCAAAACGTTCAATTTCCTGGGCCCATGTCCCAGTT 480


1311R-1.IR_full       481 TTCCCGTTCATGAGAGTTCNCC 502
                          ||||||||||||||||||| ||
1311R-1.in silico     481 TTCCCGTTCATGAGAGTTCGCC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139627.1	CG1311-RA (CG1311), mRNA
0	0	3	4	3	NM_166318.1	CG15105-RB, transcript variant B (CG15105), mRNA
0	0	3	4	3	NM_137546.2	CG15105-RA, transcript variant A (CG15105), mRNA
0	0	3	2	4	NM_168309.1	CG5263-RB, transcript variant B (smg), mRNA
0	0	3	2	4	NM_079263.2	CG5263-RA, transcript variant A (smg), mRNA
0	0	3	2	4	NM_168310.1	CG5263-RC, transcript variant C (smg), mRNA
0	0	2	3	4	NM_080376.1	CG8118-RA, transcript variant A (mam), mRNA
0	0	2	3	4	NM_166030.1	CG8118-RB, transcript variant B (mam), mRNA
0	0	2	2	3	NM_079073.1	CG8595-RA (Toll-7), mRNA
0	0	1	5	11	NM_166141.2	CG8421-RD, transcript variant D (Asph), mRNA
0	0	1	5	9	NM_079033.2	CG8421-RB, transcript variant B (Asph), mRNA
0	0	1	5	9	NM_166140.1	CG8421-RA, transcript variant A (Asph), mRNA
0	0	1	4	2	NM_135238.2	CG10354-RA (CG10354), mRNA
0	0	1	4	2	NM_141654.1	CG16790-RA (CG16790), mRNA
0	0	1	3	4	NM_001042930.1	CG17490-RB, transcript variant B (CG17490), mRNA
0	0	1	3	2	NM_168102.1	CG32239-RA (Gef64C), mRNA
0	0	1	2	5	NM_166056.1	CG10110-RB, transcript variant B (cpsf), mRNA
0	0	1	2	5	NM_206111.1	CG10110-RA, transcript variant A (cpsf), mRNA
0	0	1	2	3	NM_175991.1	CG10595-RA, transcript variant A (d), mRNA
0	0	1	2	3	NM_205939.1	CG10595-RC, transcript variant C (d), mRNA
0	0	1	2	3	NM_205940.1	CG10595-RB, transcript variant B (d), mRNA
0	0	0	7	28	NM_167065.1	CG32744-RA (CG32744), mRNA
0	0	0	7	8	NM_169627.1	CG4264-RD, transcript variant D (Hsc70-4), mRNA
0	0	0	7	8	NM_169625.1	CG4264-RB, transcript variant B (Hsc70-4), mRNA
0	0	0	7	8	NM_169626.1	CG4264-RC, transcript variant C (Hsc70-4), mRNA
0	0	0	7	8	NM_176502.1	CG4264-RE, transcript variant E (Hsc70-4), mRNA
0	0	0	7	8	NM_079632.4	CG4264-RA, transcript variant A (Hsc70-4), mRNA
0	0	0	7	8	NM_176503.1	CG4264-RF, transcript variant F (Hsc70-4), mRNA
0	0	0	6	3	NM_078638.2	CG9908-RA, transcript variant A (disco), mRNA
0	0	0	4	6	NM_167042.2	CG3187-RB, transcript variant B (Sirt4), mRNA

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