NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13116R-2

Symbol CG13116 Full Name CG13116

CG No CG13116 Old CG No CG13116

Synonyms CG13116

Accession No (Link to NCBI) NM_135462.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCGAGCTGATCACGGTGCTGG

Primer Seq. 3'

aatctagaggtaccCCTCAAGCTGGGACTCGGACT

Predicted Fragment Size

IR fragment full Seq

0001 TCGAGCTGAT CACGGTGCTG GTGTATTGGG TGATCATCAC GCCCACCACC TACAAGTACT 
0061 GCACACAAAA CGGAATAATC AGGATGAGCT TTGACACCCT GGTGGGCACT GTGGGCGATC 
0121 ACTGCACCCT GGCCCTCTGC CTGATGATCG GATATGTTGT CTTCTACCGT ATCATTATCT 
0181 TCATTTACAT GAAGTTGAAG GTCTGTGACA TTCGGATGTG GGATGAACTG CAGCGAGCCA 
0241 AAGAGGATCC GGAGGCGCAT TTGGACAAAA AGTCCTACTA CCAGCCCGTC TCCACCGAGA 
0301 ATATCGATGT CGTGGACTCT GGCAAGAAGA AGAACAAGCC AACGACCCCG AAGATGATAA 
0361 GAACCATCAG CAATCCGCTG CTTATCGAAA GCGATCTGGC GCGCATTCAC ATCAAGCACG 
0421 AAACACGGCC TTTGGAAAGT GCCACCCTAC CCAGGCTGCA GCAGGCAATG GTCCACCCTA 
0481 GTCCGAGTCC CAGCTTGAGG

in silico PCR Fragment

0001 TCGAGCTGAT CACGGTGCTG GTGTATTGGG TGATCATCAC GCCCACCACC TACAAGTACT 
0061 GCACACAAAA CGGAATAATT AGGATGAGCT TTGACACCCT GGTTGGCACT GTGGGCGATC 
0121 ACTGCACCCT GGCCCTCTGC CTGATGATCG GATACGTTGT CTTCTACCGT ATCATTATCT 
0181 TCATTTACAT GAAGTTGAAG GTCTGTGACA TTCGGATGTG GGATGAACTG CAGCGAGCCA 
0241 AAGAGGATCC GGAGGCGCAT CTGGACAAAA AGTCCTACTA CCAGCCCGTC TCCACCGAGA 
0301 ATATCGATGT CGTGGACTCT GGCAAGAAGA AGAACAAGCC AACGACTCCG AAGATGATAA 
0361 GGACCATCAG CAATCCGCTG CTCATCGAAA GCGATCTGGC GCGCATTCAC ATCAAGCACG 
0421 AAACACGGCC TTTGGAAAGT GCCACCCTAC CCAGACTGCA GCAGGCAATG GTCCACCCTA 
0481 GTCCGAGTCC CAGCTTGAGG

Assemble Data


13116R-2.IR_full       1   TCGAGCTGATCACGGTGCTGGTGTATTGGGTGATCATCACGCCCACCACCTACAAGTACT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13116R-2.in silico     1   TCGAGCTGATCACGGTGCTGGTGTATTGGGTGATCATCACGCCCACCACCTACAAGTACT 60


13116R-2.IR_full       61  GCACACAAAACGGAATAATCAGGATGAGCTTTGACACCCTGGTGGGCACTGTGGGCGATC 120
                           ||||||||||||||||||| ||||||||||||||||||||||| ||||||||||||||||
13116R-2.in silico     61  GCACACAAAACGGAATAATTAGGATGAGCTTTGACACCCTGGTTGGCACTGTGGGCGATC 120


13116R-2.IR_full       121 ACTGCACCCTGGCCCTCTGCCTGATGATCGGATATGTTGTCTTCTACCGTATCATTATCT 180
                           |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
13116R-2.in silico     121 ACTGCACCCTGGCCCTCTGCCTGATGATCGGATACGTTGTCTTCTACCGTATCATTATCT 180


13116R-2.IR_full       181 TCATTTACATGAAGTTGAAGGTCTGTGACATTCGGATGTGGGATGAACTGCAGCGAGCCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13116R-2.in silico     181 TCATTTACATGAAGTTGAAGGTCTGTGACATTCGGATGTGGGATGAACTGCAGCGAGCCA 240


13116R-2.IR_full       241 AAGAGGATCCGGAGGCGCATTTGGACAAAAAGTCCTACTACCAGCCCGTCTCCACCGAGA 300
                           |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
13116R-2.in silico     241 AAGAGGATCCGGAGGCGCATCTGGACAAAAAGTCCTACTACCAGCCCGTCTCCACCGAGA 300


13116R-2.IR_full       301 ATATCGATGTCGTGGACTCTGGCAAGAAGAAGAACAAGCCAACGACCCCGAAGATGATAA 360
                           |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
13116R-2.in silico     301 ATATCGATGTCGTGGACTCTGGCAAGAAGAAGAACAAGCCAACGACTCCGAAGATGATAA 360


13116R-2.IR_full       361 GAACCATCAGCAATCCGCTGCTTATCGAAAGCGATCTGGCGCGCATTCACATCAAGCACG 420
                           | |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
13116R-2.in silico     361 GGACCATCAGCAATCCGCTGCTCATCGAAAGCGATCTGGCGCGCATTCACATCAAGCACG 420


13116R-2.IR_full       421 AAACACGGCCTTTGGAAAGTGCCACCCTACCCAGGCTGCAGCAGGCAATGGTCCACCCTA 480
                           |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
13116R-2.in silico     421 AAACACGGCCTTTGGAAAGTGCCACCCTACCCAGACTGCAGCAGGCAATGGTCCACCCTA 480


13116R-2.IR_full       481 GTCCGAGTCCCAGCTTGAGG 500
                           ||||||||||||||||||||
13116R-2.in silico     481 GTCCGAGTCCCAGCTTGAGG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_135462.2	CG13116-RA (CG13116), mRNA
0.2	1	3	2	4	NM_080325.3	CG6450-RC (lva), mRNA
0.2	1	3	2	3	NM_132004.2	CG4136-RA (CG4136), mRNA
0	0	2	7	2	NM_136274.2	CG2225-RC, transcript variant C (CG2225), mRNA
0	0	2	7	2	NM_206020.2	CG2225-RE, transcript variant E (CG2225), mRNA
0	0	2	7	2	NM_165392.2	CG2225-RA, transcript variant A (CG2225), mRNA
0	0	2	7	2	NM_165393.2	CG2225-RB, transcript variant B (CG2225), mRNA
0	0	2	7	2	NM_001014497.1	CG2225-RF, transcript variant F (CG2225), mRNA
0	0	2	6	7	NM_169810.1	CG18617-RA, transcript variant A (Vha100-2), mRNA
0	0	2	6	7	NM_142465.1	CG18617-RB, transcript variant B (Vha100-2), mRNA
0	0	2	3	3	NM_142354.1	CG4090-RA (CG4090), mRNA
0	0	2	3	2	NM_136758.2	CG12309-RA (CG12309), mRNA
0	0	2	2	3	NM_141178.3	CG12581-RA, transcript variant A (CG12581), mRNA
0	0	2	2	3	NM_164313.1	CG12581-RB, transcript variant B (CG12581), mRNA
0	0	2	2	2	NM_136864.2	CG13185-RA (CG13185), mRNA
0	0	1	4	3	NM_136699.3	CG12128-RA, transcript variant A (CG12128), mRNA
0	0	1	4	3	NM_206068.1	CG12128-RB, transcript variant B (CG12128), mRNA
0	0	1	3	3	NM_058040.3	CG10372-RA (Faf), mRNA
0	0	1	3	3	NM_137412.2	CG6406-RB, transcript variant B (CG6406), mRNA
0	0	1	3	3	NM_166247.1	CG6406-RA, transcript variant A (CG6406), mRNA
0	0	1	2	5	NM_139522.1	CG14964-RA (CG14964), mRNA
0	0	1	2	4	NM_176583.1	CG11500-RA (Spase12), mRNA
0	0	1	2	4	NM_143436.1	CG2006-RA (CG2006), mRNA
0	0	1	2	3	NM_137983.3	CG9850-RA, transcript variant A (CG9850), mRNA
0	0	1	2	3	NM_080250.2	CG6224-RA (dbo), mRNA
0	0	1	2	2	NM_167921.1	CG12024-RB, transcript variant B (CG12024), mRNA
0	0	1	2	2	NM_139413.2	CG12024-RA, transcript variant A (CG12024), mRNA
0	0	1	2	2	NM_132006.1	CG17763-RA (CG17763), mRNA
0	0	0	5	13	NM_131944.1	CG3546-RA (CG3546), mRNA
0	0	0	5	8	NM_140150.2	CG32067-RC, transcript variant C (simj), mRNA

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