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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13072R-1 |
| Symbol |
PDCD-5 |
Full Name |
PDCD-5 |
| CG No |
CG13072 |
Old CG No |
CG13072 |
| Synonyms |
DMPDCD-5, CG13072, BcDNA:RE17056, PDCD-5 |
| Accession No (Link to NCBI) |
NM_140591.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTGGATGCCTTACGCGCGCAG |
| Primer Seq. 3' |
aatctagaggtaccCAGCCGTAATCCTCGTCGTCG |
| Predicted Fragment Size |
383 |
| IR fragment full Seq |
0001 TTGGATNGCC TTACGCNCGC AGCGCATGTC CCAAATGCAG TCGCAATTCG GCGGCGGCAA
0061 TGATGCGGAA AAGCAACAAG CCCAGCAGGA GCAGATGCGC GCCCAGGAGG AAATGAAGCA
0121 CTCGATATTA TCCCAAGTTC TGGACCAGCA GGCCAGGGCA CGACTTAATA CCCTCAAAGT
0181 CAGCAAGCCG GAGAAGGCGC AAATGTTCGA GAACATGGTC ATCCGCATGG CCCAGATGGG
0241 TTCAGGTGCG TGGAAAACTT GATGACGCCC AGTTCGTCAG CATCCTCGAA AGCGTCAATG
0301 CTCAGATGCC GCAGAGCAAG TCCTCCGTGA AATACGATCG GCGGCGGGCG GCCATCGATA
0361 GCGACGACGA CGAGGATTAC GGCTG |
| in silico PCR Fragment |
0001 TTGGAT-GCC TTACGCGCGC AGCGCATGTC CCAAATGCAG TCGCAATTCG GCGGCGGCAA
0061 TGATGCGGAA AAGCAACAAG CCCAGCAGGA GCAGATGCGC GCCCAGGAGG AAATGAAGCA
0121 CTCGATATTA TCCCAAGTTC TGGACCAGCA GGCCAGGGCA CGACTTAATA CCCTCAAAGT
0181 CAGCAAGCCG GAGAAGGCGC AAATGTTCGA GAACATGGTC ATCCGCATGG CCCAGATGGG
0241 -TCAGGTGCG TGGAAAACTT GATGACGCCC AGTTCGTCAG CATCCTCGAA AGCGTCAATG
0301 CTCAGATGCC GCAGAGCAAG TCCTCCGTGA AATACGATCG GCGGCGGGCG GCCATCGATA
0361 GCGACGACGA CGAGGATTAC GGCTG |
| Assemble Data |
13072R-1.IR_full 1 TTGGATNGCCTTACGCNCGCAGCGCATGTCCCAAATGCAGTCGCAATTCGGCGGCGGCAA 60
|||||| ||||||||| |||||||||||||||||||||||||||||||||||||||||||
13072R-1.in silico 1 TTGGAT-GCCTTACGCGCGCAGCGCATGTCCCAAATGCAGTCGCAATTCGGCGGCGGCAA 60
13072R-1.IR_full 61 TGATGCGGAAAAGCAACAAGCCCAGCAGGAGCAGATGCGCGCCCAGGAGGAAATGAAGCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13072R-1.in silico 61 TGATGCGGAAAAGCAACAAGCCCAGCAGGAGCAGATGCGCGCCCAGGAGGAAATGAAGCA 120
13072R-1.IR_full 121 CTCGATATTATCCCAAGTTCTGGACCAGCAGGCCAGGGCACGACTTAATACCCTCAAAGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13072R-1.in silico 121 CTCGATATTATCCCAAGTTCTGGACCAGCAGGCCAGGGCACGACTTAATACCCTCAAAGT 180
13072R-1.IR_full 181 CAGCAAGCCGGAGAAGGCGCAAATGTTCGAGAACATGGTCATCCGCATGGCCCAGATGGG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13072R-1.in silico 181 CAGCAAGCCGGAGAAGGCGCAAATGTTCGAGAACATGGTCATCCGCATGGCCCAGATGGG 240
13072R-1.IR_full 241 TTCAGGTGCGTGGAAAACTTGATGACGCCCAGTTCGTCAGCATCCTCGAAAGCGTCAATG 300
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13072R-1.in silico 241 -TCAGGTGCGTGGAAAACTTGATGACGCCCAGTTCGTCAGCATCCTCGAAAGCGTCAATG 300
13072R-1.IR_full 301 CTCAGATGCCGCAGAGCAAGTCCTCCGTGAAATACGATCGGCGGCGGGCGGCCATCGATA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13072R-1.in silico 301 CTCAGATGCCGCAGAGCAAGTCCTCCGTGAAATACGATCGGCGGCGGGCGGCCATCGATA 360
13072R-1.IR_full 361 GCGACGACGACGAGGATTACGGCTG 385
|||||||||||||||||||||||||
13072R-1.in silico 361 GCGACGACGACGAGGATTACGGCTG 385
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
365 |
0 |
0 |
0 |
NM_140591.2 |
CG13072-RA (PDCD-5), mRNA |
| 0 |
0 |
8 |
3 |
5 |
NM_165261.1 |
CG10336-RA, transcript variant A (CG10336), mRNA |
| 0 |
0 |
8 |
3 |
5 |
NM_136051.1 |
CG10336-RB, transcript variant B (CG10336), mRNA |
| 0 |
0 |
3 |
5 |
3 |
NM_137450.1 |
CG17680-RA (CG17680), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_078516.2 |
CG12690-RA (CHES-1-like), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_131919.1 |
CG15376-RA (CG15376), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_078605.1 |
CG6211-RA (gce), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_135998.1 |
CG5094-RA (Sgt), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_170166.2 |
CG13627-RB, transcript variant B (CG13627), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_143022.2 |
CG13627-RA, transcript variant A (CG13627), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_164941.1 |
CG17118-RA (CG17118), mRNA |
| 0 |
0 |
1 |
8 |
11 |
NM_166404.1 |
CG16742-RB, transcript variant B (CG16742), mRNA |
| 0 |
0 |
1 |
8 |
11 |
NM_137652.2 |
CG16742-RA, transcript variant A (CG16742), mRNA |
| 0 |
0 |
1 |
4 |
10 |
NM_176271.1 |
CG2469-RB, transcript variant B (CG2469), mRNA |
| 0 |
0 |
1 |
4 |
10 |
NM_176270.1 |
CG2469-RA, transcript variant A (CG2469), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_079729.2 |
CG4792-RA (Dcr-1), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_135145.1 |
CG9144-RA (CG9144), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_140102.1 |
CG14173-RA (Ilp1), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168398.1 |
CG32057-RB, transcript variant B (dpr10), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168397.1 |
CG32057-RA, transcript variant A (dpr10), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168399.1 |
CG32057-RC, transcript variant C (dpr10), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_135239.1 |
CG18304-RA (CG18304), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_136693.2 |
CG12130-RA (CG12130), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_001042832.1 |
CG41476-RA (CG41476), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_130605.3 |
CG3806-RA, transcript variant A (eIF2B-epsilon), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_206606.1 |
CG3806-RB, transcript variant B (eIF2B-epsilon), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167831.1 |
CG1228-RD, transcript variant D (Ptpmeg), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167832.2 |
CG1228-RB, transcript variant B (Ptpmeg), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167830.1 |
CG1228-RA, transcript variant A (Ptpmeg), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_138187.2 |
CG1228-RC, transcript variant C (Ptpmeg), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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