NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 1303R-3

Symbol agt Full Name O-6-alkylguanine-DNA alkyltransferase

CG No CG1303 Old CG No CG1303

Synonyms DmAGT, CG1303, BG:DS00004.10, agt, Agt

Accession No (Link to NCBI) NM_058018.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTGGCCAAAACACCCAGATTC

Primer Seq. 3'

aatctagaggtaccTCCGTTTTGGGACACAACTCG

Predicted Fragment Size

IR fragment full Seq

0001 TTGGCCAAAA CACCCAGATT CGTTTGGTGC CACTAAAGGA TCCGCCAAAG ACCATCCGAT 
0061 ACAGTTTCCT GGCGACCAAG TTCGGGCAGA TGATGATGGG AGTGATAAAC CCAATATCCG 
0121 GCGATTCGAA AGACCAAGTC GCAATTGGTG TGCTTCANTT CGTGATTAAG GACAATGTGA 
0181 GTACCTACAA TGAAGTCCAG GAGCGGTGGC CAAAATCCGA ACTCTGGAAG GACGATGATG 
0241 CGGTCAAGTT GGTGGCGGAT AAACTGTTCG AAAGCGACAG AGGAAAACTC GCAGGCGATT 
0301 CCCATTGCCA TCTATGGAAC CGACTTCCAG CTGTCCGTAT GGCGAGCTCT GGTGCATATG 
0361 AAACGAGGGG AGACCTGCAC TTACTCCCAA CTGGCCGAGC GGATGGGTCG TCCAACGGCG 
0421 GTCAGAGCAG TGGCCAGTGC TGTGGCCAAA AATGAGCTTG CTATCCTCAT TCCGTGCCAC 
0481 CGAGTTGTGT CCCAAAACNG A

in silico PCR Fragment

0001 TTGGCCAAAA CACCCAGATT CGTTTGGTGC CACTAAAGGA TCCGCCAAAG ACCATCCGAT 
0061 ACAGTTTCCT GGCGACCAAG TTCGGGCAGA TGATGATGGG AGTGATAAAC CCAATATCCG 
0121 GCGATTCGAA AGACCAAGTC GCAATTGGTG TGCTTCACTT CGTGATTAAG GACAATGTGA 
0181 GTACCTACAA TGAAGTCCAG GAGCGGTGGC CAAAATCCGA ACTCTGGAAG GACGATGATG 
0241 CGGTCAAGTT GGTGGCGGAT AAACTGTTCG AAAGCGACA- AGGAAAACTC GCAGGCGATT 
0301 CCCATTGCCA TCTATGGAAC CGACTTCCAG CTGTCCGTAT GGCGAGCTCT GGTGCATATG 
0361 AAACGAGGGG AGACCTGCAC TTACTCCCAA CTGGCCGAGC GGATGGGTCG TCCAACGGCG 
0421 GTCAGAGCAG TGGCCAGTGC TGTGGCCAAA AATGAGCTTG CTATCCTCAT TCCGTGCCAC 
0481 CGAGTTGTGT CCCAAAACGG A

Assemble Data


1303R-3.IR_full       1   TTGGCCAAAACACCCAGATTCGTTTGGTGCCACTAAAGGATCCGCCAAAGACCATCCGAT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1303R-3.in silico     1   TTGGCCAAAACACCCAGATTCGTTTGGTGCCACTAAAGGATCCGCCAAAGACCATCCGAT 60


1303R-3.IR_full       61  ACAGTTTCCTGGCGACCAAGTTCGGGCAGATGATGATGGGAGTGATAAACCCAATATCCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1303R-3.in silico     61  ACAGTTTCCTGGCGACCAAGTTCGGGCAGATGATGATGGGAGTGATAAACCCAATATCCG 120


1303R-3.IR_full       121 GCGATTCGAAAGACCAAGTCGCAATTGGTGTGCTTCANTTCGTGATTAAGGACAATGTGA 180
                          ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
1303R-3.in silico     121 GCGATTCGAAAGACCAAGTCGCAATTGGTGTGCTTCACTTCGTGATTAAGGACAATGTGA 180


1303R-3.IR_full       181 GTACCTACAATGAAGTCCAGGAGCGGTGGCCAAAATCCGAACTCTGGAAGGACGATGATG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1303R-3.in silico     181 GTACCTACAATGAAGTCCAGGAGCGGTGGCCAAAATCCGAACTCTGGAAGGACGATGATG 240


1303R-3.IR_full       241 CGGTCAAGTTGGTGGCGGATAAACTGTTCGAAAGCGACAGAGGAAAACTCGCAGGCGATT 300
                          ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
1303R-3.in silico     241 CGGTCAAGTTGGTGGCGGATAAACTGTTCGAAAGCGACA-AGGAAAACTCGCAGGCGATT 300


1303R-3.IR_full       301 CCCATTGCCATCTATGGAACCGACTTCCAGCTGTCCGTATGGCGAGCTCTGGTGCATATG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1303R-3.in silico     301 CCCATTGCCATCTATGGAACCGACTTCCAGCTGTCCGTATGGCGAGCTCTGGTGCATATG 360


1303R-3.IR_full       361 AAACGAGGGGAGACCTGCACTTACTCCCAACTGGCCGAGCGGATGGGTCGTCCAACGGCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1303R-3.in silico     361 AAACGAGGGGAGACCTGCACTTACTCCCAACTGGCCGAGCGGATGGGTCGTCCAACGGCG 420


1303R-3.IR_full       421 GTCAGAGCAGTGGCCAGTGCTGTGGCCAAAAATGAGCTTGCTATCCTCATTCCGTGCCAC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1303R-3.in silico     421 GTCAGAGCAGTGGCCAGTGCTGTGGCCAAAAATGAGCTTGCTATCCTCATTCCGTGCCAC 480


1303R-3.IR_full       481 CGAGTTGTGTCCCAAAACNGA 501
                          |||||||||||||||||| ||
1303R-3.in silico     481 CGAGTTGTGTCCCAAAACGGA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_058018.2	CG1303-RA (agt), mRNA
0	0	3	3	5	NM_132311.2	CG12119-RA (CG12119), mRNA
0	0	3	2	4	NM_141005.1	CG3618-RA (CG3618), mRNA
0	0	2	3	3	NM_135669.2	CG4983-RA (CG4983), mRNA
0	0	2	2	2	NM_141702.2	CG12806-RA (Teh1), mRNA
0	0	1	5	4	NM_137652.2	CG16742-RA, transcript variant A (CG16742), mRNA
0	0	1	5	4	NM_166404.1	CG16742-RB, transcript variant B (CG16742), mRNA
0	0	1	4	8	NM_167193.1	CG32702-RA (CG32702), mRNA
0	0	1	4	5	NM_206443.1	CG1030-RB, transcript variant B (Scr), mRNA
0	0	1	4	5	NM_079524.3	CG1030-RA, transcript variant A (Scr), mRNA
0	0	1	4	5	NM_206442.1	CG1030-RC, transcript variant C (Scr), mRNA
0	0	1	3	6	NM_079223.2	CG10129-RA (ndl), mRNA
0	0	1	2	5	NM_132087.3	CG15893-RA (CG15893), mRNA
0	0	1	2	3	NM_136211.3	CG9320-RA (CG9320), mRNA
0	0	1	2	3	NM_143605.2	CG11339-RA (CG11339), mRNA
0	0	1	2	3	NM_165285.1	CG31797-RA (CG31797), mRNA
0	0	1	2	2	NM_132079.1	CG15897-RA (CG15897), mRNA
0	0	1	2	2	NM_165836.1	CG7776-RB, transcript variant B (E(Pc)), mRNA
0	0	1	2	2	NM_078974.2	CG7776-RA, transcript variant A (E(Pc)), mRNA
0	0	0	7	2	NM_001043293.1	CG34103-RA (BG642167), mRNA
0	0	0	5	4	NM_132543.1	CG10362-RA (CG10362), mRNA
0	0	0	4	7	NM_057361.3	CG4316-RA (Sb), mRNA
0	0	0	4	5	NM_079134.5	CG9071-RA, transcript variant A (NaCP60E), mRNA
0	0	0	4	5	NM_166696.1	CG9071-RB, transcript variant B (NaCP60E), mRNA
0	0	0	4	3	NM_137649.2	CG30147-RA, transcript variant A (Hil), mRNA
0	0	0	4	3	NM_166402.1	CG30147-RB, transcript variant B (Hil), mRNA
0	0	0	4	2	NM_138988.2	CG11405-RA (A3-3), mRNA
0	0	0	4	2	NM_078877.3	CG31794-RA, transcript variant A (Pax), mRNA
0	0	0	3	7	NM_167326.1	CG1848-RA, transcript variant A (LIMK1), mRNA
0	0	0	3	7	NM_140250.1	CG5964-RA (CG5964), mRNA

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