NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13005R-2

Symbol CG13005 Full Name CG13005

CG No CG13005 Old CG No CG13005

Synonyms CG13005

Accession No (Link to NCBI) NM_132940.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGCTGCCGGATAAGACCGGTG

Primer Seq. 3'

aatctagaggtaccGCGGGCAGAACTTGGCTCAAC

Predicted Fragment Size

IR fragment full Seq

0001 TGCTGCCGGA TAAGACCGGT GGTGTACAGG ATNCGACAGG AGGCCGCCAA GAATNCCTGC 
0061 AGAGAATTCC GTGCCCGACG AAGATTTGCC GGCTGCAGGA GGCGATTTGC TACGCGTTAA 
0121 GGATACTCTG GGCCAGCTGG CCTGCAGTCT GCACAAGCTG TCCAAAAACG ATCACATAGT 
0181 CATCGATCGA AAGCGCCATC AGGTGATTCG CACAGATGCG GCTACGCAAC AATTAACGAT 
0241 TTACCGCTGT CCGGATCCCG CCGTCATGGG GATGTCGCCC GGCGATAGAG AAAAATGCCC 
0301 AGAATCCGCC TGCCAAAGTG GGTAGACCAT CGAAAAGAGC CTGTTCGGTT GCCACCGCTC 
0361 CTGTGGCGAA GAAACCAACA CTAGCGGAGC AAAAACCTGC TCCCGACACC CATCGCACTA 
0421 GATCCGGTCG CCAGGTGCGC AGCTTGAATG CCACAGCCCG CTCCCGTAAC CAGTGGCTCA 
0481 GCGGTTGAGC CAAGTTCTGC CCGC

in silico PCR Fragment

0001 TGCTGCCGGA TAAGACCGGT GGTGTACAGG AT-CGACAGG AGGCCGCCAA GAAT-CCTGC 
0061 AGAGAATTCC GTGCCCGACG AAGATTTGCC GGCTGCAGGA GGCGATTTGC TACGCGTTAA 
0121 GGATACTCTG GGCCAGCTGG CCTGCAGTCT GCACAAGCTG TCCAAAAACG ATCACATAGT 
0181 CATCGATCGA AAGCGCCATC AGGTGATTCG CACAGATGCG GCTACGCAAC AATTAACGAT 
0241 TTACCGCTGT CCGGATCCCG CCGTCATGGG GAT-TCGCCC GGCGATAGAG AAAAATGCCC 
0301 AGAATCCGCC TGCCAAAGTG GGTAGACCAT CGAAAAGAGC CTGTTCGGTT GCCACCGCTC 
0361 CTGTGGCGAA GAAACCAACA CTAGCGGAGC AAAAACCTGC TCCCGACACC CATCGCACTA 
0421 GATCCGGTCG CCAGGTGCGC AGCTTG-ATG CCACAGCCCG CTCCCGTGAC CAGTGGCTCA 
0481 GCGGTTGAGC CAAGTTCTGC CCGC

Assemble Data


13005R-2.IR_full       1   TGCTGCCGGATAAGACCGGTGGTGTACAGGATNCGACAGGAGGCCGCCAAGAATNCCTGC 60
                           |||||||||||||||||||||||||||||||| ||||||||||||||||||||| |||||
13005R-2.in silico     1   TGCTGCCGGATAAGACCGGTGGTGTACAGGAT-CGACAGGAGGCCGCCAAGAAT-CCTGC 60


13005R-2.IR_full       61  AGAGAATTCCGTGCCCGACGAAGATTTGCCGGCTGCAGGAGGCGATTTGCTACGCGTTAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13005R-2.in silico     61  AGAGAATTCCGTGCCCGACGAAGATTTGCCGGCTGCAGGAGGCGATTTGCTACGCGTTAA 120


13005R-2.IR_full       121 GGATACTCTGGGCCAGCTGGCCTGCAGTCTGCACAAGCTGTCCAAAAACGATCACATAGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13005R-2.in silico     121 GGATACTCTGGGCCAGCTGGCCTGCAGTCTGCACAAGCTGTCCAAAAACGATCACATAGT 180


13005R-2.IR_full       181 CATCGATCGAAAGCGCCATCAGGTGATTCGCACAGATGCGGCTACGCAACAATTAACGAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13005R-2.in silico     181 CATCGATCGAAAGCGCCATCAGGTGATTCGCACAGATGCGGCTACGCAACAATTAACGAT 240


13005R-2.IR_full       241 TTACCGCTGTCCGGATCCCGCCGTCATGGGGATGTCGCCCGGCGATAGAGAAAAATGCCC 300
                           ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
13005R-2.in silico     241 TTACCGCTGTCCGGATCCCGCCGTCATGGGGAT-TCGCCCGGCGATAGAGAAAAATGCCC 300


13005R-2.IR_full       301 AGAATCCGCCTGCCAAAGTGGGTAGACCATCGAAAAGAGCCTGTTCGGTTGCCACCGCTC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13005R-2.in silico     301 AGAATCCGCCTGCCAAAGTGGGTAGACCATCGAAAAGAGCCTGTTCGGTTGCCACCGCTC 360


13005R-2.IR_full       361 CTGTGGCGAAGAAACCAACACTAGCGGAGCAAAAACCTGCTCCCGACACCCATCGCACTA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13005R-2.in silico     361 CTGTGGCGAAGAAACCAACACTAGCGGAGCAAAAACCTGCTCCCGACACCCATCGCACTA 420


13005R-2.IR_full       421 GATCCGGTCGCCAGGTGCGCAGCTTGAATGCCACAGCCCGCTCCCGTAACCAGTGGCTCA 480
                           |||||||||||||||||||||||||| |||||||||||||||||||| ||||||||||||
13005R-2.in silico     421 GATCCGGTCGCCAGGTGCGCAGCTTG-ATGCCACAGCCCGCTCCCGTGACCAGTGGCTCA 480


13005R-2.IR_full       481 GCGGTTGAGCCAAGTTCTGCCCGC 504
                           ||||||||||||||||||||||||
13005R-2.in silico     481 GCGGTTGAGCCAAGTTCTGCCCGC 504

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_132940.1	CG13005-RA (CG13005), mRNA
0	0	1	4	2	NM_132550.2	CG1796-RA (Tango4), mRNA
0	0	1	3	2	NM_140870.1	CG9300-RA (CG9300), mRNA
0	0	1	2	7	NM_168802.1	CG32210-RA (CG32210), mRNA
0	0	1	2	3	NM_132068.1	CG15899-RB (Ca-alpha1T), mRNA
0	0	1	2	2	NM_134834.2	CG9867-RA (CG9867), mRNA
0	0	1	2	2	NM_168271.1	CG7118-RA (Jon66Ci), mRNA
0	0	0	6	2	NM_080010.2	CG11606-RA (Rpp30), mRNA
0	0	0	4	9	NM_057354.2	CG8930-RA, transcript variant A (rk), mRNA
0	0	0	4	9	NM_134277.1	CG8930-RD, transcript variant D (rk), mRNA
0	0	0	4	9	NM_134275.1	CG8930-RB, transcript variant B (rk), mRNA
0	0	0	4	9	NM_134276.1	CG8930-RC, transcript variant C (rk), mRNA
0	0	0	4	9	NM_176034.1	CG8930-RE, transcript variant E (rk), mRNA
0	0	0	4	6	NM_132521.2	CG1578-RA (CG1578), mRNA
0	0	0	4	5	NM_134485.3	CG12788-RA, transcript variant A (CG12788), mRNA
0	0	0	4	5	NM_001031903.1	CG17767-RC, transcript variant C (Tim9b), mRNA
0	0	0	4	3	NM_137942.1	CG13550-RA (CG13550), mRNA
0	0	0	4	3	NM_079495.2	CG17947-RA (alpha-Cat), mRNA
0	0	0	3	12	NM_136687.2	CG1472-RA (sec24), mRNA
0	0	0	3	6	NM_078523.2	CG2252-RB, transcript variant B (fs(1)h), mRNA
0	0	0	3	5	NM_144369.2	CG18578-RA (Ugt86Da), mRNA
0	0	0	3	3	NM_057374.2	CG3722-RA (shg), mRNA
0	0	0	3	3	NM_001031932.1	CG7971-RD, transcript variant D (CG7971), mRNA
0	0	0	3	3	NM_139385.3	CG7971-RA, transcript variant A (CG7971), mRNA
0	0	0	3	3	NM_165630.1	CG8266-RB, transcript variant B (sec31), mRNA
0	0	0	3	3	NM_136570.2	CG8266-RA, transcript variant A (sec31), mRNA
0	0	0	3	3	NM_167909.2	CG7971-RC, transcript variant C (CG7971), mRNA
0	0	0	3	3	NM_057916.3	CG3619-RA, transcript variant A (Dl), mRNA
0	0	0	3	3	NM_169853.1	CG3619-RB, transcript variant B (Dl), mRNA
0	0	0	3	3	NM_167910.2	CG7971-RB, transcript variant B (CG7971), mRNA

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