NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 12983R-4

Symbol CG12983 Full Name CG12983

CG No CG12983 Old CG No CG12983

Synonyms bs29a07.y1, NEST:bs29a07, CG12983

Accession No (Link to NCBI) NM_141017.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal semi-lethal

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCTTCCAGCTGGCATGTTTC

Primer Seq. 3'

aatctagaggtaccTTTCGCTTTCGACGGCGTTTC

Predicted Fragment Size

IR fragment full Seq

0001 AGCTTCCAGC TGGCATGTTT CCTGANCCAC GCAAGATATT TCTGGAGCAC GAAAAACGAG 
0061 ATTGCACTGA CTTCTTTAAT AGATACTTCC ATCCAGGCAA TATAAATTTA CTGCCCTCAG 
0121 AGGTGAACCT GCGTCGTTTC ATCATCTTAG GTGGCATAGT CTCGTTGGTC TTTGTGGGAA 
0181 AAGCCAAACA TGCCTCTTAT GAGAAATTTA ATATAACGCT GCATGAGGAT GGACGTGTAC 
0241 TGCGGAAATC TCTAAATATT TTGGACGAAT CGAGTACGGA GGAATGTAGT ATGCAGCCTA 
0301 GCAAATTGGA TATCGGCACA GATATAATGA GGGATACCGA AGTTGGGGAA GCCTCTAAAA 
0361 TTCCACTGCA CTTGGAACCA GATGAGCTGC CTTATTACTT TCTCACCTTC AAGGTGCCCA 
0421 ATCATTTGTG CTTGTGGGGA GAACCCATTG TTTGCCAATT CTTCGAGAGC GAAATCGAAG 
0481 AAACGCCGTC GAAAGCGAAA

in silico PCR Fragment

0001 AGCTTCCAGC TGGCATGTTT CCTGATCCAC GCAAGATATT TCTGGAGCAC GAAAAACGAG 
0061 ATTGCACTGA CTTCTTTAAT AGATACTTCC ATCCAGGCAA TATAAATTTA CTGCCCTCAG 
0121 AGGTGAACCT GCGTCGTTTC ATCATCTTAG GTGGCATAGT CTCGTTGGTC TTTGTGGGAA 
0181 AAGCCAAACA TGCCTCTTAT GAGAAATTTA ATATAACGCT GCATGAGGAT GGACGTGTAC 
0241 TGCGGAAATC TCTAAATATT TTGGACGAAT CGAGTACGGA GGAATGTAGT ATGCAGCCTA 
0301 GCAAATTGGA TATCGGCACA GATATAATGA GGGATACCGA AGTTGGGGAA GCCTCTAAAA 
0361 TTCCACTGCA CTTGGAACCA GATGAGCTGC CTTATTACTT TCTCACCTTC AAGGTGCCCA 
0421 ATCATTTGTG CTTGTGGGGA GAACCCATTG TTTGCCAATT CTTCGAGAGC GAAATCGAAG 
0481 AAACGCCGTC GAAAGCGAAA

Assemble Data


12983R-4.IR_full       1   AGCTTCCAGCTGGCATGTTTCCTGANCCACGCAAGATATTTCTGGAGCACGAAAAACGAG 60
                           ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
12983R-4.in silico     1   AGCTTCCAGCTGGCATGTTTCCTGATCCACGCAAGATATTTCTGGAGCACGAAAAACGAG 60


12983R-4.IR_full       61  ATTGCACTGACTTCTTTAATAGATACTTCCATCCAGGCAATATAAATTTACTGCCCTCAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12983R-4.in silico     61  ATTGCACTGACTTCTTTAATAGATACTTCCATCCAGGCAATATAAATTTACTGCCCTCAG 120


12983R-4.IR_full       121 AGGTGAACCTGCGTCGTTTCATCATCTTAGGTGGCATAGTCTCGTTGGTCTTTGTGGGAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12983R-4.in silico     121 AGGTGAACCTGCGTCGTTTCATCATCTTAGGTGGCATAGTCTCGTTGGTCTTTGTGGGAA 180


12983R-4.IR_full       181 AAGCCAAACATGCCTCTTATGAGAAATTTAATATAACGCTGCATGAGGATGGACGTGTAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12983R-4.in silico     181 AAGCCAAACATGCCTCTTATGAGAAATTTAATATAACGCTGCATGAGGATGGACGTGTAC 240


12983R-4.IR_full       241 TGCGGAAATCTCTAAATATTTTGGACGAATCGAGTACGGAGGAATGTAGTATGCAGCCTA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12983R-4.in silico     241 TGCGGAAATCTCTAAATATTTTGGACGAATCGAGTACGGAGGAATGTAGTATGCAGCCTA 300


12983R-4.IR_full       301 GCAAATTGGATATCGGCACAGATATAATGAGGGATACCGAAGTTGGGGAAGCCTCTAAAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12983R-4.in silico     301 GCAAATTGGATATCGGCACAGATATAATGAGGGATACCGAAGTTGGGGAAGCCTCTAAAA 360


12983R-4.IR_full       361 TTCCACTGCACTTGGAACCAGATGAGCTGCCTTATTACTTTCTCACCTTCAAGGTGCCCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12983R-4.in silico     361 TTCCACTGCACTTGGAACCAGATGAGCTGCCTTATTACTTTCTCACCTTCAAGGTGCCCA 420


12983R-4.IR_full       421 ATCATTTGTGCTTGTGGGGAGAACCCATTGTTTGCCAATTCTTCGAGAGCGAAATCGAAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12983R-4.in silico     421 ATCATTTGTGCTTGTGGGGAGAACCCATTGTTTGCCAATTCTTCGAGAGCGAAATCGAAG 480


12983R-4.IR_full       481 AAACGCCGTCGAAAGCGAAA 500
                           ||||||||||||||||||||
12983R-4.in silico     481 AAACGCCGTCGAAAGCGAAA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_141017.2	CG12983-RA (CG12983), mRNA
0	0	0	6	6	NM_080421.3	CG18730-RA (Amy-p), mRNA
0	0	0	5	7	NM_079336.2	CG7002-RA (Hml), mRNA
0	0	0	4	5	NM_079044.1	CG17876-RA (Amy-d), mRNA
0	0	0	4	5	NM_166765.1	CG11533-RC, transcript variant C (Asator), mRNA
0	0	0	4	5	NM_166766.1	CG11533-RB, transcript variant B (Asator), mRNA
0	0	0	4	5	NM_143667.1	CG11533-RA, transcript variant A (Asator), mRNA
0	0	0	4	4	NM_170449.2	CG31038-RD, transcript variant D (CG31038), mRNA
0	0	0	4	4	NM_170448.1	CG31038-RB, transcript variant B (CG31038), mRNA
0	0	0	4	2	NM_142982.2	CG13611-RA (CG13611), mRNA
0	0	0	3	16	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA
0	0	0	3	5	NM_137809.1	CG3292-RA (CG3292), mRNA
0	0	0	3	4	NM_057760.4	CG12245-RA (gcm), mRNA
0	0	0	3	4	NM_132599.1	CG12717-RA (CG12717), mRNA
0	0	0	3	4	NM_079275.2	CG4463-RA (Hsp23), mRNA
0	0	0	3	4	NM_169084.1	CG31546-RA (CG31546), mRNA
0	0	0	3	3	NM_137730.2	CG30387-RA, transcript variant A (CG30387), mRNA
0	0	0	3	3	NM_080142.2	CG1925-RA (mus205), mRNA
0	0	0	3	2	NM_136595.1	CG13744-RA (CG13744), mRNA
0	0	0	3	2	NM_001043084.1	CG34123-RD, transcript variant D (CG34123), mRNA
0	0	0	3	2	NM_166441.2	CG30387-RB, transcript variant B (CG30387), mRNA
0	0	0	3	2	NM_166442.2	CG30387-RC, transcript variant C (CG30387), mRNA
0	0	0	3	2	NM_141553.1	CG9770-RA (CG9770), mRNA
0	0	0	3	2	NM_165324.1	CG10076-RD, transcript variant D (spir), mRNA
0	0	0	3	2	NM_165019.2	CG5792-RB, transcript variant B (CG5792), mRNA
0	0	0	2	12	NM_205875.1	CG32019-RE, transcript variant E (bt), mRNA
0	0	0	2	12	NM_205876.1	CG32019-RC, transcript variant C (bt), mRNA
0	0	0	2	12	NM_205877.1	CG32019-RD, transcript variant D (bt), mRNA
0	0	0	2	12	NM_166790.2	CG32019-RA, transcript variant A (bt), mRNA
0	0	0	2	11	NM_206404.1	CG33287-RA (CG33287), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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