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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12934R-1 
 Symbol CG12934  Full Name CG12934 
 CG No CG12934  Old CG No CG12934 
 Synonyms CG12934 
 Accession No (Link to NCBI) NM_136757.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGTTTTGACGGCTCCGCCAGT 
 Primer Seq. 3' 
aatctagaggtaccTTTCCAAGTGCTGTGGAGGCT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGTTTTGACG GCTCCGCCAG TCAGGGTGAT GCCGAAACCA GGCAGTGAGC TTGATCAGTA 
0061 CGCTCACATA TTTGCAAAGG TACGACCACC GGGCCAGAAG CACCAGAACA TGGAGATTGA 
0121 ATTCCAGCCA CAGCACCACA ACTCTCTGCC GAGCAACCAG GTCAATGCGG AAATCAATCA 
0181 TGTGGAGCCC AAGCAGCCTC AGTACATGGA ACAAAGTTCT TCTCAGGTCG AGTCGAGACC 
0241 TCCTCACACC GTGGACTTTG AGATCAAGCC GGTGGAGCAG AAGCCACCCC AGCGATTGGA 
0301 GTTTGAGGTG AANGCCGCAG CACGAGCAAA ACTCTCCTCA GGGACATTTG GTTTCTTTGG 
0361 GCGTCAAGCC CGTGNGAGCA GAAGCCACCT CAGCACTTGG AGGTGGAGAT TAAGCAGCAG 
0421 CAGCAACAAC ATTCTTCGCA AAATCATCAG GTGGAACTGG AAATCAAGCA GGTGGAACAG 
0481 AAGCCTCCAC AGCACTTGGA AA 
 in silico PCR Fragment 
0001 AGTTTTGACG GCTCCGCCAG TCAGGGTGAT GCCGAAACCA GGCAGTGAGC TTGATCAGTA 
0061 CGCTCACATA TTTGCAAAGG TACGACCACC GGGCCAGAAG CACCAGAACA TGGAGATTGA 
0121 ATTCCAGCCA CAGCACCACA ACTCTCTGCC GAGCAACCAG GTCAATGCGG AAATCAATCA 
0181 TGTGGAGCCC AAGCAGCCTC AGTACATGGA ACAAAGTTCT TCTCAGGTCG AGTCGAGACC 
0241 TCCTCACACC GTGGACTTTG AGATCAAGCC GGTGGAGCAG AAGCCACCCC AGCGATTGGA 
0301 GTTTGAGGTG AA-GCCGCAG CACGAGCAAA ACTCTCCTCA GGGACATTTG GTTTCTTTGG 
0361 GCGTCAAGCC CGTG-GAGCA GAAGCCACCT CAGCACTTGG AGGTGGAGAT TAAGCAGCAG 
0421 CAGCAACAAC ATTCTTCGCA AAATCATCAG GTGGAACTGG AAATCAAGCA GGTGGAACAG 
0481 AAGCCTCCAC AGCACTTGGA AA 
 Assemble Data 

12934R-1.IR_full       1   AGTTTTGACGGCTCCGCCAGTCAGGGTGATGCCGAAACCAGGCAGTGAGCTTGATCAGTA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12934R-1.in silico     1   AGTTTTGACGGCTCCGCCAGTCAGGGTGATGCCGAAACCAGGCAGTGAGCTTGATCAGTA 60


12934R-1.IR_full       61  CGCTCACATATTTGCAAAGGTACGACCACCGGGCCAGAAGCACCAGAACATGGAGATTGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12934R-1.in silico     61  CGCTCACATATTTGCAAAGGTACGACCACCGGGCCAGAAGCACCAGAACATGGAGATTGA 120


12934R-1.IR_full       121 ATTCCAGCCACAGCACCACAACTCTCTGCCGAGCAACCAGGTCAATGCGGAAATCAATCA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12934R-1.in silico     121 ATTCCAGCCACAGCACCACAACTCTCTGCCGAGCAACCAGGTCAATGCGGAAATCAATCA 180


12934R-1.IR_full       181 TGTGGAGCCCAAGCAGCCTCAGTACATGGAACAAAGTTCTTCTCAGGTCGAGTCGAGACC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12934R-1.in silico     181 TGTGGAGCCCAAGCAGCCTCAGTACATGGAACAAAGTTCTTCTCAGGTCGAGTCGAGACC 240


12934R-1.IR_full       241 TCCTCACACCGTGGACTTTGAGATCAAGCCGGTGGAGCAGAAGCCACCCCAGCGATTGGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12934R-1.in silico     241 TCCTCACACCGTGGACTTTGAGATCAAGCCGGTGGAGCAGAAGCCACCCCAGCGATTGGA 300


12934R-1.IR_full       301 GTTTGAGGTGAANGCCGCAGCACGAGCAAAACTCTCCTCAGGGACATTTGGTTTCTTTGG 360
                           |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
12934R-1.in silico     301 GTTTGAGGTGAA-GCCGCAGCACGAGCAAAACTCTCCTCAGGGACATTTGGTTTCTTTGG 360


12934R-1.IR_full       361 GCGTCAAGCCCGTGNGAGCAGAAGCCACCTCAGCACTTGGAGGTGGAGATTAAGCAGCAG 420
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
12934R-1.in silico     361 GCGTCAAGCCCGTG-GAGCAGAAGCCACCTCAGCACTTGGAGGTGGAGATTAAGCAGCAG 420


12934R-1.IR_full       421 CAGCAACAACATTCTTCGCAAAATCATCAGGTGGAACTGGAAATCAAGCAGGTGGAACAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12934R-1.in silico     421 CAGCAACAACATTCTTCGCAAAATCATCAGGTGGAACTGGAAATCAAGCAGGTGGAACAG 480


12934R-1.IR_full       481 AAGCCTCCACAGCACTTGGAAA 502
                           ||||||||||||||||||||||
12934R-1.in silico     481 AAGCCTCCACAGCACTTGGAAA 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  27  34  120  NM_136757.1  CG12934-RA (CG12934), mRNA 
1.24   6  17  64  167  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
1.24   6  17  64  167  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
1.03   5  48  162  384  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
1.03   5  48  162  384  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
1.03   5  48  162  384  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
1.03   5  12  35  77  NM_078592.2  CG11172-RA (NFAT), mRNA 
0.82   4  30  77  176  NM_168571.2  CG32133-RA (CG32133), mRNA 
0.82   4  15  35  90  NM_206286.1  CG10107-RC, transcript variant C (CG10107), mRNA 
0.82   4  15  35  90  NM_139799.3  CG10107-RA, transcript variant A (CG10107), mRNA 
0.82   4  14  35  73  NM_134474.4  CG32532-RA (CG32532), mRNA 
0.82   4  14  33  64  NM_169696.1  CG3992-RA, transcript variant A (srp), mRNA 
0.82   4  14  33  64  NM_169694.1  CG3992-RB, transcript variant B (srp), mRNA 
0.82   4  13  30  74  NM_132246.2  CG10555-RA (CG10555), mRNA 
0.82   4  10  26  61  NM_079939.2  CG5580-RA, transcript variant A (sbb), mRNA 
0.82   4  7  19  58  NM_169025.1  CG2534-RB, transcript variant B (cno), mRNA 
0.82   4  7  19  58  NM_079508.2  CG2534-RA, transcript variant A (cno), mRNA 
0.82   4  6  21  74  NM_132235.2  CG32717-RB, transcript variant B (sdt), mRNA 
0.82   4  5  15  56  NM_206653.1  CG32717-RE, transcript variant E (sdt), mRNA 
0.82   4  5  15  56  NM_132236.3  CG32717-RA, transcript variant A (sdt), mRNA 
0.62   3  17  64  142  NM_135077.2  CG14023-RA (CG14023), mRNA 
0.62   3  16  40  82  NM_137690.2  CG10543-RA, transcript variant A (CG10543), mRNA 
0.62   3  16  34  59  NM_166416.1  CG10543-RB, transcript variant B (CG10543), mRNA 
0.62   3  16  34  59  NM_176241.1  CG10543-RC, transcript variant C (CG10543), mRNA 
0.62   3  12  43  83  NM_176557.1  CG33106-RB, transcript variant B (mask), mRNA 
0.62   3  12  43  83  NM_176556.1  CG33106-RA, transcript variant A (mask), mRNA 
0.62   3  12  29  49  NM_168412.1  CG32062-RD, transcript variant D (CG32062), mRNA 
0.62   3  12  29  49  NM_168413.1  CG32062-RB, transcript variant B (CG32062), mRNA 
0.62   3  9  24  63  NM_132126.1  CG3075-RA (CG3075), mRNA 
0.62   3  9  14  29  NM_132606.1  CG3754-RA (CG3754), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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