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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12927R-2 
 Symbol CG34364  Full Name - 
 CG No CG34364  Old CG No CG12927 
 Synonyms FBgn0085393, CG33479, CG1863, CG12927, CG34364 
 Accession No (Link to NCBI) NM_136662.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAACTGTCCAGGGCGGCACTAT 
 Primer Seq. 3' 
aatctagaggtaccCATTCGATTCGACACCCCGCT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AACTGTCCAG GGCGGCACTA TAAATCGTCT CCATACAACC CCTGAAAGCC AGGTGNCGNA 
0061 TGTGCCTGTG GcCAAGTGGA TCCATGGAGC AGCCTACCTT TACGGGAACC ACAGTATTTC 
0121 TGGCATTACT CATCTGAATA ATGTCAATCT CTACAACGAT CTTACCGTGA ACGGGTCTGT 
0181 AAATGGTGTC CCCTGGCAGG CGGACAAGCT CATTTTGATG GACAATGAGC AGGATATACC 
0241 GGGCTACCCT GCTGGTGGCA AATAGTCTTC CCGAACAGAA GCGAGTTATG AGCAATAATG 
0301 TGAAGAATCT CTGGGTGGAT TTCGTCAACG ATCTGCCCGT TAACGAGTTA CTGGTCAGCA 
0361 AGGCAGAAAA CCGTCCCAAT CTTCACGTGG AAAGCCAACT GATCTTTACC CAGCCCCTCA 
0421 CTGTGGGTCA CTACGAAGCG GGCAACGGAA ATGGGCTGCT GGATAAATAT TACAAGAAAA 
0481 AGCGGGGTGT CGAATCGAAT G 
 in silico PCR Fragment 
0001 AACTGTCCAG GGCGGCACTA TAAATCGTCT CCATACAACC CCTGAAAGCC AGGTGGCGGA 
0061 TGTGCCTGTG GCCAAGTGGA TCCATGGAGC AGCCTACCTT TACGGGAACC ACAGTATTTC 
0121 TGGCATTACT CATCTGAATA ATGTCAATCT CTACAACGAT CTTACCGTGA ACGGGTCTGT 
0181 AAATGGTGTC CCCTGGCAGG CGGACAAGCT CATTTTGATG GACAATGAGC AGGATATACC 
0241 GGGCT-CCCT GCTGGTGGCG AATAGTCTTC CCGAACAGAA GCGAGTTATG AGCAATAATG 
0301 TGAAGAATCT CTGGGTGGAT TTCGTCAACG ATCTGCCCGT TAACGAGTTA CTGGTCAGCA 
0361 AGGCAGAAAA CCGTCCCAAT CTTCACGTGG AAAGCCAACT GATCTTCACC CAGCCCCTCA 
0421 CTGTGGGTCA CTACGAAGCG GGCAACGGAA ATGGGCTGCT GGATAAATAT TACAAGAAAA 
0481 AGCGGGGTGT CGAATCGAAT G 
 Assemble Data 

12927R-2.IR_full       1   AACTGTCCAGGGCGGCACTATAAATCGTCTCCATACAACCCCTGAAAGCCAGGTGNCGNA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||| || |
12927R-2.in silico     1   AACTGTCCAGGGCGGCACTATAAATCGTCTCCATACAACCCCTGAAAGCCAGGTGGCGGA 60


12927R-2.IR_full       61  TGTGCCTGTGGCCAAGTGGATCCATGGAGCAGCCTACCTTTACGGGAACCACAGTATTTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12927R-2.in silico     61  TGTGCCTGTGGCCAAGTGGATCCATGGAGCAGCCTACCTTTACGGGAACCACAGTATTTC 120


12927R-2.IR_full       121 TGGCATTACTCATCTGAATAATGTCAATCTCTACAACGATCTTACCGTGAACGGGTCTGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12927R-2.in silico     121 TGGCATTACTCATCTGAATAATGTCAATCTCTACAACGATCTTACCGTGAACGGGTCTGT 180


12927R-2.IR_full       181 AAATGGTGTCCCCTGGCAGGCGGACAAGCTCATTTTGATGGACAATGAGCAGGATATACC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12927R-2.in silico     181 AAATGGTGTCCCCTGGCAGGCGGACAAGCTCATTTTGATGGACAATGAGCAGGATATACC 240


12927R-2.IR_full       241 GGGCTACCCTGCTGGTGGCAAATAGTCTTCCCGAACAGAAGCGAGTTATGAGCAATAATG 300
                           ||||| ||||||||||||| ||||||||||||||||||||||||||||||||||||||||
12927R-2.in silico     241 GGGCT-CCCTGCTGGTGGCGAATAGTCTTCCCGAACAGAAGCGAGTTATGAGCAATAATG 300


12927R-2.IR_full       301 TGAAGAATCTCTGGGTGGATTTCGTCAACGATCTGCCCGTTAACGAGTTACTGGTCAGCA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12927R-2.in silico     301 TGAAGAATCTCTGGGTGGATTTCGTCAACGATCTGCCCGTTAACGAGTTACTGGTCAGCA 360


12927R-2.IR_full       361 AGGCAGAAAACCGTCCCAATCTTCACGTGGAAAGCCAACTGATCTTTACCCAGCCCCTCA 420
                           |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
12927R-2.in silico     361 AGGCAGAAAACCGTCCCAATCTTCACGTGGAAAGCCAACTGATCTTCACCCAGCCCCTCA 420


12927R-2.IR_full       421 CTGTGGGTCACTACGAAGCGGGCAACGGAAATGGGCTGCTGGATAAATATTACAAGAAAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12927R-2.in silico     421 CTGTGGGTCACTACGAAGCGGGCAACGGAAATGGGCTGCTGGATAAATATTACAAGAAAA 480


12927R-2.IR_full       481 AGCGGGGTGTCGAATCGAATG 501
                           |||||||||||||||||||||
12927R-2.in silico     481 AGCGGGGTGTCGAATCGAATG 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  4  NM_136662.1  CG12927-RA (CG12927), mRNA 
0   0  2  2  2  NM_058162.2  CG1633-RA, transcript variant A (Jafrac1), mRNA 
0   0  2  2  2  NM_167359.1  CG1633-RB, transcript variant B (Jafrac1), mRNA 
0   0  1  4  4  NM_137451.2  CG5742-RA (CG5742), mRNA 
0   0  1  3  4  NM_001032080.1  CG33695-RA, transcript variant A (CG33695), mRNA 
0   0  1  3  4  NM_001032077.1  CG33695-RE, transcript variant E (CG33695), mRNA 
0   0  1  3  4  NM_001032079.1  CG33695-RC, transcript variant C (CG33695), mRNA 
0   0  1  3  4  NM_001032081.1  CG33695-RF, transcript variant F (CG33695), mRNA 
0   0  1  3  4  NM_001032078.1  CG33695-RD, transcript variant D (CG33695), mRNA 
0   0  1  3  4  NM_001032076.1  CG33694-RA, transcript variant A (cana), mRNA 
0   0  1  3  2  NM_057920.3  CG3423-RA (SA), mRNA 
0   0  1  2  3  NM_132955.1  CG4937-RA, transcript variant A (RhoGAP15B), mRNA 
0   0  1  2  3  NM_132755.1  CG9509-RA (CG9509), mRNA 
0   0  1  2  3  NM_001042817.1  CG4937-RB, transcript variant B (RhoGAP15B), mRNA 
0   0  0  5  7  NM_078982.2  CG8344-RA (RpIII128), mRNA 
0   0  0  5  6  NM_140396.1  CG10738-RB, transcript variant B (CG10738), mRNA 
0   0  0  5  6  NM_168547.1  CG10738-RA, transcript variant A (CG10738), mRNA 
0   0  0  5  5  NM_057270.3  CG7123-RA, transcript variant A (LanB1), mRNA 
0   0  0  5  5  NM_164773.1  CG7123-RB, transcript variant B (LanB1), mRNA 
0   0  0  5  2  NM_137883.1  CG13535-RA (CG13535), mRNA 
0   0  0  4  4  NM_137047.2  CG6197-RA (CG6197), mRNA 
0   0  0  4  2  NM_135136.1  CG13996-RA (CG13996), mRNA 
0   0  0  4  2  NM_143632.2  CG2196-RA (CG2196), mRNA 
0   0  0  3  9  NM_001032051.1  CG33715-RD, transcript variant D (Msp-300), mRNA 
0   0  0  3  8  NM_206404.1  CG33287-RA (CG33287), mRNA 
0   0  0  3  5  NM_166019.1  CG18076-RH, transcript variant H (shot), mRNA 
0   0  0  3  5  NM_141666.2  CG9492-RA (CG9492), mRNA 
0   0  0  3  5  NM_080512.2  CG5247-RA (Irbp), mRNA 
0   0  0  3  4  NM_140341.1  CG10969-RA (CG10969), mRNA 
0   0  0  3  3  NM_133127.1  CG7453-RA, transcript variant A (CG7453), mRNA 

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