|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1288R-2 |
| Symbol |
CG1288 |
Full Name |
CG1288 |
| CG No |
CG1288 |
Old CG No |
CG1288 |
| Synonyms |
BEST:LP10010, BcDNA:AT26093, CG1288 |
| Accession No (Link to NCBI) |
NM_141448.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGAGATCGTCGATCTGCGAGT |
| Primer Seq. 3' |
aatctagaggtaccTGGCGAAGTTCCGGATTCGGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGAGATCGTC GATCTGCGAG TCNCTGATTA TCTGAGAACT CTGCTGCAGG ACATCCTGTG
0061 TTTCCTGGTC CTGCTCTCGG TGGCCTTGCT AATTGTGGCT GGCATTTTGT TTGTGGTGGA
0121 GGACGTAAGC CGGCTCCAGC AGCAGCACCA ACACCAGCCG TACCACCACC ACCATCATCG
0181 CCACATGCGT CCGGATCNGA TAGCCAATGA ACTAAAAGGA TCGCCGATCG AGGAGGCACC
0241 GAGGGAGCAA GGTCAAGTCT TCCGACCGCA CACATGGATT CGATTCTTTA ACTGCAGGAA
0301 CAAGGTGCCA CAGCAACAGC AGGAAGAAGT TGAAAGGGAG GCGGTTCAGT TTGGCCCACC
0361 AACGCAAGAC GATCAGGCGC AGCAGATCCC ATTAGATGCC GTTCAAACTT ATCGCCCTGC
0421 CATAGAACCT ATATCGCTGT CGCTCTGGCG GAGATTACAT AGATGAAACG ATCAACAAAA
0481 ACCCGAATCC GGNACTTCGC CA |
| in silico PCR Fragment |
0001 GGAGATCGTC GATCTGCGAG TC-CTGATTA TCTGAGAACT CTGCTGCAGG ACATCCTGTG
0061 TTTCCTGGTC CTGCTCTCGG TGGCCTTGCT AATTGTGGCT GGCATTTTGT TTGTGGTGGA
0121 GGACGTAAGC CGGCTCCAGC AGCAGCACCA ACACCAGCCG TACCACCACC ACCATCATCG
0181 CCACATGCGT CCGGATCAGA TAGCCAATGA ACTAAAAGGA TCGCCGATCG AGGAGGCACC
0241 GAGGGAGCAA GGTCAAGTCT TCCGACCGCA CACATGGATT CGATTCTTTA ACTGCAGGAA
0301 CAAGGTGCCA CAGCAACAGC AGGAAAAAGT TGAAAGGGAG GCGGTTCAGT TTGGCCCACC
0361 AACGCAAGAC GATCAGGCGC AGCAGATCCC ATTAGATGCC GTTCAAACTT ATCGCCCTGC
0421 CATAGAACCT ATATCGCTG- CGCTCTGGCG GAGATTACAT AGATGAAACG ATCAACAAAA
0481 ACCCGAATCC GGAACTTCGC CA |
| Assemble Data |
1288R-2.IR_full 1 GGAGATCGTCGATCTGCGAGTCNCTGATTATCTGAGAACTCTGCTGCAGGACATCCTGTG 60
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
1288R-2.in silico 1 GGAGATCGTCGATCTGCGAGTC-CTGATTATCTGAGAACTCTGCTGCAGGACATCCTGTG 60
1288R-2.IR_full 61 TTTCCTGGTCCTGCTCTCGGTGGCCTTGCTAATTGTGGCTGGCATTTTGTTTGTGGTGGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1288R-2.in silico 61 TTTCCTGGTCCTGCTCTCGGTGGCCTTGCTAATTGTGGCTGGCATTTTGTTTGTGGTGGA 120
1288R-2.IR_full 121 GGACGTAAGCCGGCTCCAGCAGCAGCACCAACACCAGCCGTACCACCACCACCATCATCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1288R-2.in silico 121 GGACGTAAGCCGGCTCCAGCAGCAGCACCAACACCAGCCGTACCACCACCACCATCATCG 180
1288R-2.IR_full 181 CCACATGCGTCCGGATCNGATAGCCAATGAACTAAAAGGATCGCCGATCGAGGAGGCACC 240
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
1288R-2.in silico 181 CCACATGCGTCCGGATCAGATAGCCAATGAACTAAAAGGATCGCCGATCGAGGAGGCACC 240
1288R-2.IR_full 241 GAGGGAGCAAGGTCAAGTCTTCCGACCGCACACATGGATTCGATTCTTTAACTGCAGGAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1288R-2.in silico 241 GAGGGAGCAAGGTCAAGTCTTCCGACCGCACACATGGATTCGATTCTTTAACTGCAGGAA 300
1288R-2.IR_full 301 CAAGGTGCCACAGCAACAGCAGGAAGAAGTTGAAAGGGAGGCGGTTCAGTTTGGCCCACC 360
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
1288R-2.in silico 301 CAAGGTGCCACAGCAACAGCAGGAAAAAGTTGAAAGGGAGGCGGTTCAGTTTGGCCCACC 360
1288R-2.IR_full 361 AACGCAAGACGATCAGGCGCAGCAGATCCCATTAGATGCCGTTCAAACTTATCGCCCTGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1288R-2.in silico 361 AACGCAAGACGATCAGGCGCAGCAGATCCCATTAGATGCCGTTCAAACTTATCGCCCTGC 420
1288R-2.IR_full 421 CATAGAACCTATATCGCTGTCGCTCTGGCGGAGATTACATAGATGAAACGATCAACAAAA 480
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
1288R-2.in silico 421 CATAGAACCTATATCGCTG-CGCTCTGGCGGAGATTACATAGATGAAACGATCAACAAAA 480
1288R-2.IR_full 481 ACCCGAATCCGGNACTTCGCCA 502
|||||||||||| |||||||||
1288R-2.in silico 481 ACCCGAATCCGGAACTTCGCCA 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_141448.2 |
CG1288-RA (CG1288), mRNA |
| 1.03 |
5 |
2 |
8 |
55 |
NM_140200.1 |
CG6175-RB (CG6175), mRNA |
| 0.41 |
2 |
3 |
15 |
47 |
NM_169142.1 |
CG10272-RB, transcript variant B (gpp), mRNA |
| 0.41 |
2 |
3 |
15 |
47 |
NM_141398.1 |
CG10272-RA, transcript variant A (gpp), mRNA |
| 0.41 |
2 |
3 |
15 |
47 |
NM_169143.1 |
CG10272-RD, transcript variant D (gpp), mRNA |
| 0.41 |
2 |
3 |
15 |
45 |
NM_169144.1 |
CG10272-RC, transcript variant C (gpp), mRNA |
| 0.41 |
2 |
3 |
6 |
37 |
NM_165232.1 |
CG5674-RB, transcript variant B (CG5674), mRNA |
| 0.41 |
2 |
3 |
6 |
37 |
NM_136012.4 |
CG5674-RA, transcript variant A (CG5674), mRNA |
| 0.41 |
2 |
3 |
6 |
37 |
NM_165233.1 |
CG5674-RC, transcript variant C (CG5674), mRNA |
| 0.41 |
2 |
3 |
5 |
34 |
NM_206153.1 |
CG33130-RC, transcript variant C (l(2)k07433), mRNA |
| 0.41 |
2 |
3 |
5 |
34 |
NM_176218.1 |
CG33130-RA, transcript variant A (l(2)k07433), mRNA |
| 0.41 |
2 |
3 |
5 |
34 |
NM_206154.1 |
CG33130-RB, transcript variant B (l(2)k07433), mRNA |
| 0.41 |
2 |
3 |
4 |
7 |
NM_206125.1 |
CG8295-RD, transcript variant D (Mlf), mRNA |
| 0.41 |
2 |
3 |
4 |
7 |
NM_166122.2 |
CG8295-RC, transcript variant C (Mlf), mRNA |
| 0.2 |
1 |
5 |
18 |
66 |
NM_080025.1 |
CG11387-RA, transcript variant A (ct), mRNA |
| 0.2 |
1 |
4 |
12 |
95 |
NM_080364.3 |
CG5461-RA, transcript variant A (bun), mRNA |
| 0.2 |
1 |
4 |
11 |
86 |
NM_001042894.1 |
CG5461-RF, transcript variant F (bun), mRNA |
| 0 |
0 |
12 |
99 |
547 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 0 |
0 |
12 |
99 |
547 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 0 |
0 |
12 |
99 |
547 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 0 |
0 |
8 |
18 |
96 |
NM_142210.1 |
CG6118-RA (CG6118), mRNA |
| 0 |
0 |
8 |
16 |
61 |
NM_136647.2 |
CG8809-RA (Camta), mRNA |
| 0 |
0 |
8 |
3 |
15 |
NM_079475.2 |
CG32443-RA (Pc), mRNA |
| 0 |
0 |
7 |
46 |
100 |
NM_079996.2 |
CG18024-RA (SoxN), mRNA |
| 0 |
0 |
7 |
29 |
97 |
NM_169696.1 |
CG3992-RA, transcript variant A (srp), mRNA |
| 0 |
0 |
7 |
29 |
97 |
NM_169694.1 |
CG3992-RB, transcript variant B (srp), mRNA |
| 0 |
0 |
7 |
17 |
45 |
NM_132175.2 |
CG12155-RA (CG12155), mRNA |
| 0 |
0 |
7 |
11 |
67 |
NM_142889.1 |
CG4374-RA (CG4374), mRNA |
| 0 |
0 |
6 |
50 |
215 |
NM_080376.1 |
CG8118-RA, transcript variant A (mam), mRNA |
| 0 |
0 |
6 |
50 |
215 |
NM_166030.1 |
CG8118-RB, transcript variant B (mam), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
|
|
|
|
