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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12807R-2 
 Symbol CG12807  Full Name CG12807 
 CG No CG12807  Old CG No CG12807 
 Synonyms CG12807 
 Accession No (Link to NCBI) NM_141708.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGAGGTGATGGTGGACCTGAC 
 Primer Seq. 3' 
aatctagaggtaccTTGTTGTGGCCGGCTCTTCTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGAgGTGATG GTGGACCTGA CCAACGACCT GACCCACCGC CTGCTCCACT ACCATTCCAT 
0061 CCTGAACCGC AACAACTTCG CCTTTCTCGC CCACTGCCTT GGTCAGTGTG CTGGTGGCTC 
0121 TGTTCGAGGG TTCGGCCGGG AATACCGCCG AGGNAACTGC GCCATGTCCT GCAGCTGCCC 
0181 AACAACAGGG ATGTGATCCC GCGTGGGCTA CCGCGACATC CATCGACGAC TGCGGACTTA 
0241 CTTTTTTGGC TCCGACAATC CACTGAAAGG ACTCAGCTTG AACAAGGGCA ATGTCACTGT 
0301 TCTCAAGGAC TTTGAGACGG TTCTTATGTT TTACGGATAT GACTTAAGTG TAGATATGCT 
0361 CTCCTCCACA CCAGCCAATC TCACATCAGA TGCCGAGTTG GTCAACACCA CAATGGCCAG 
0421 CAATACGACG ACCATGGAAA TGGATGCGGA AACCACAACG TCAAAGGATG CGGAGAGCAC 
0481 AACAGAAGAG CCGGCCACAA CAA 
 in silico PCR Fragment 
0001 CGAGGTGATG GTGGACCTGA CCAACGACCT GACCCACCGC CTGCTCCACT ACCATTCCAT 
0061 CCTGAACCGC AACAACTTCG CC-TTCTCGC CCACTGCCTT GGTCAGTGTG CTGGTGGCTC 
0121 TGTTCGAGGG TTCGGCCGGG AATACCGCCG AGG-AACTGC GCCATGTCCT GCAGCTGCCC 
0181 AACAACAGGG ATGTGA-CCC GCGTGGGCTA CCGCGACATC CATCGACGAC TGCGGACTTA 
0241 CTTTTTTGGC TCCGACAATC CACTGAAAGG ACTCAGCTTG AACAAGGGCA ATGTCACTGT 
0301 TCTCAAGGAC TTTGAGACGG TTCTTATGTT TTACGGATAT GACTTAAGTG TAGATATGCT 
0361 CTCCTCCACA CCAGCCAATC TCACATCAGA TGCCGAGTTG GTCAACACCA CAATGGCCAG 
0421 CAATACGACG ACCATGGAAA TGGATGCGGA AACCACAACG TCAAAGGATG CGGAGAGCAC 
0481 AACAGAAGAG CCGGCCACAA CAA 
 Assemble Data 

12807R-2.IR_full       1   CGAGGTGATGGTGGACCTGACCAACGACCTGACCCACCGCCTGCTCCACTACCATTCCAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12807R-2.in silico     1   CGAGGTGATGGTGGACCTGACCAACGACCTGACCCACCGCCTGCTCCACTACCATTCCAT 60


12807R-2.IR_full       61  CCTGAACCGCAACAACTTCGCCTTTCTCGCCCACTGCCTTGGTCAGTGTGCTGGTGGCTC 120
                           |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
12807R-2.in silico     61  CCTGAACCGCAACAACTTCGCC-TTCTCGCCCACTGCCTTGGTCAGTGTGCTGGTGGCTC 120


12807R-2.IR_full       121 TGTTCGAGGGTTCGGCCGGGAATACCGCCGAGGNAACTGCGCCATGTCCTGCAGCTGCCC 180
                           ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
12807R-2.in silico     121 TGTTCGAGGGTTCGGCCGGGAATACCGCCGAGG-AACTGCGCCATGTCCTGCAGCTGCCC 180


12807R-2.IR_full       181 AACAACAGGGATGTGATCCCGCGTGGGCTACCGCGACATCCATCGACGACTGCGGACTTA 240
                           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
12807R-2.in silico     181 AACAACAGGGATGTGA-CCCGCGTGGGCTACCGCGACATCCATCGACGACTGCGGACTTA 240


12807R-2.IR_full       241 CTTTTTTGGCTCCGACAATCCACTGAAAGGACTCAGCTTGAACAAGGGCAATGTCACTGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12807R-2.in silico     241 CTTTTTTGGCTCCGACAATCCACTGAAAGGACTCAGCTTGAACAAGGGCAATGTCACTGT 300


12807R-2.IR_full       301 TCTCAAGGACTTTGAGACGGTTCTTATGTTTTACGGATATGACTTAAGTGTAGATATGCT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12807R-2.in silico     301 TCTCAAGGACTTTGAGACGGTTCTTATGTTTTACGGATATGACTTAAGTGTAGATATGCT 360


12807R-2.IR_full       361 CTCCTCCACACCAGCCAATCTCACATCAGATGCCGAGTTGGTCAACACCACAATGGCCAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12807R-2.in silico     361 CTCCTCCACACCAGCCAATCTCACATCAGATGCCGAGTTGGTCAACACCACAATGGCCAG 420


12807R-2.IR_full       421 CAATACGACGACCATGGAAATGGATGCGGAAACCACAACGTCAAAGGATGCGGAGAGCAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12807R-2.in silico     421 CAATACGACGACCATGGAAATGGATGCGGAAACCACAACGTCAAAGGATGCGGAGAGCAC 480


12807R-2.IR_full       481 AACAGAAGAGCCGGCCACAACAA 503
                           |||||||||||||||||||||||
12807R-2.in silico     481 AACAGAAGAGCCGGCCACAACAA 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  8  NM_141708.2  CG12807-RA (CG12807), mRNA 
0   0  3  3  10  NM_139732.2  CG10542-RA (CG10542), mRNA 
0   0  3  2  3  NM_139392.1  CG7991-RA (CG7991), mRNA 
0   0  2  3  3  NM_142461.2  CG14309-RA (CG14309), mRNA 
0   0  2  2  7  NM_166745.1  CG31999-RA (CG31999), mRNA 
0   0  1  5  7  NM_170326.2  CG31068-RA (CG31068), mRNA 
0   0  1  3  5  NM_137235.2  CG8399-RA (CG8399), mRNA 
0   0  1  3  4  NM_078616.3  CG10524-RA (Pkcdelta), mRNA 
0   0  1  3  3  NM_140784.1  CG7271-RA (CG7271), mRNA 
0   0  1  3  3  NM_080143.3  CG4216-RA (term), mRNA 
0   0  1  3  2  NM_135509.3  CG5734-RA (CG5734), mRNA 
0   0  1  3  2  NM_136209.2  CG9318-RA (CG9318), mRNA 
0   0  1  2  5  NM_132788.2  CG9114-RA (CG9114), mRNA 
0   0  1  2  5  NM_139680.1  CG17150-RA, transcript variant A (CG17150), mRNA 
0   0  1  2  4  NM_078924.3  CG12165-RA (Incenp), mRNA 
0   0  1  2  4  NM_140898.3  CG32217-RA (Su(Tpl)), mRNA 
0   0  0  7  3  NM_001032271.1  CG13430-RA, transcript variant A (CG13430), mRNA 
0   0  0  7  3  NM_001032270.1  CG13430-RB, transcript variant B (CG13430), mRNA 
0   0  0  7  3  NM_166398.1  CG18065-RA, transcript variant A (CG18065), mRNA 
0   0  0  6  2  NM_167380.1  CG32628-RA (CG32628), mRNA 
0   0  0  5  4  NM_169947.1  CG17299-RF, transcript variant F (SNF4Agamma), mRNA 
0   0  0  5  4  NM_169464.2  CG10097-RA, transcript variant A (CG10097), mRNA 
0   0  0  5  4  NM_001032011.1  CG10097-RB, transcript variant B (CG10097), mRNA 
0   0  0  5  4  NM_141929.2  CG10096-RA, transcript variant A (CG10096), mRNA 
0   0  0  5  3  NM_140048.2  CG4080-RA (CG4080), mRNA 
0   0  0  5  3  NM_132660.2  CG1716-RA (CG1716), mRNA 
0   0  0  5  3  NM_167847.1  CG18214-RC, transcript variant C (trio), mRNA 
0   0  0  5  2  NM_143703.2  CG18214-RA, transcript variant A (trio), mRNA 
0   0  0  5  2  NM_080509.2  CG17299-RB, transcript variant B (SNF4Agamma), mRNA 
0   0  0  5  2  NM_169946.1  CG17299-RA, transcript variant A (SNF4Agamma), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
+ 
VM
+ 
VB
 
Dcompartment   Penetrance
     

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