National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock discarded request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12716R-2 
 Symbol CG12716  Full Name CG12716 
 CG No CG12716  Old CG No CG12716 
 Synonyms CG12716 
 Accession No (Link to NCBI) NM_132617.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGACGCAGCAGAAACGGAAAG 
 Primer Seq. 3' 
aatctagaggtaccGGCGAATGTTTGTGGAATAGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGACGCAGCA GAAACGGAAA GTTAGCAACA GTCGAGCTTT TACCATGATT TTAATCCTGC 
0061 TGCAAGTTCT TGGTATCCTT GGCCGTGGAG TGTTGGCCCA GAAAAGCTCG CCCATTGCCA 
0121 ACTGGCACAT GGCTGATCCG CACTACACAA GTGACCAGTA CGCCAAAATT CTGGGCGAAG 
0181 CGGGTCTGGG CCAGGATGCC GATGACAAGG AGCACAAGGG TCTGGGTCCC ATGCAAATCC 
0241 AGTACGTGGA CTACCAGCCA AACTGCCAGC CGGGCGGAGT GCCAGTCTGT GCCACCAATG 
0301 GAACCGATAG CTTCTACTTC GAGAATCATT GCCGTTTGGA GGCGGCCAAC ATGAAGATGC 
0361 TCTTCCAGCA CGGCACAGAA CTGGAGCCCA CGGAGATGGA GCGATGCCTG CCCAACTGCC 
0421 AGACGATGAA GTGCACCCAG GTTGAGCGTC CAGTTTGCGC GCTGGCCGAG ATTGGAGGAG 
0481 CTATTCCACA AACATTCGCC  
 in silico PCR Fragment 
0001 AGACGCAGCA GAAACGGAAA GTTAGCAACA GTCGAGCTTT TACCATGATT TTAATCCTGC 
0061 TGCAAGTTCT TGGTATCCTT GGCCGTGGAG TGTTGGCCCA GAAAAGCTCG CCCATTGCCA 
0121 ACTGGCACAT GGCTGATCCG CACTACACAA GTGACCAGTA CGCCAAAATT CTGGGCGAAG 
0181 CGGGTCTGGG CCAGGATGCC GATGACAAGG AGCACAAGGG TCTGGGTCCC ATGCAAATCC 
0241 AGTACGTGGA CTACCAGCCA AACTGCCAGC CGGGCGGAGT GCCAGTCTGT GCCACCAATG 
0301 GAACCGATAG CTTCTACTTC GAGAATCATT GCCGTTTGGA GGCGGCCAAC ATGAAGATGC 
0361 TCTTCCAGCA CGGCACAGAA CTGGAGCCCA CGGAGATGGA GCGATGCCTG CCCAACTGCC 
0421 AGACGATGAA GTGCACCCAG GTTGAGCGTC CAGTTTGCGC GCTGGCCGAG ATTGGAGGAG 
0481 CTATTCCACA AACATTCGCC  
 Assemble Data 

12716R-2.IR_full       1   AGACGCAGCAGAAACGGAAAGTTAGCAACAGTCGAGCTTTTACCATGATTTTAATCCTGC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12716R-2.in silico     1   AGACGCAGCAGAAACGGAAAGTTAGCAACAGTCGAGCTTTTACCATGATTTTAATCCTGC 60


12716R-2.IR_full       61  TGCAAGTTCTTGGTATCCTTGGCCGTGGAGTGTTGGCCCAGAAAAGCTCGCCCATTGCCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12716R-2.in silico     61  TGCAAGTTCTTGGTATCCTTGGCCGTGGAGTGTTGGCCCAGAAAAGCTCGCCCATTGCCA 120


12716R-2.IR_full       121 ACTGGCACATGGCTGATCCGCACTACACAAGTGACCAGTACGCCAAAATTCTGGGCGAAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12716R-2.in silico     121 ACTGGCACATGGCTGATCCGCACTACACAAGTGACCAGTACGCCAAAATTCTGGGCGAAG 180


12716R-2.IR_full       181 CGGGTCTGGGCCAGGATGCCGATGACAAGGAGCACAAGGGTCTGGGTCCCATGCAAATCC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12716R-2.in silico     181 CGGGTCTGGGCCAGGATGCCGATGACAAGGAGCACAAGGGTCTGGGTCCCATGCAAATCC 240


12716R-2.IR_full       241 AGTACGTGGACTACCAGCCAAACTGCCAGCCGGGCGGAGTGCCAGTCTGTGCCACCAATG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12716R-2.in silico     241 AGTACGTGGACTACCAGCCAAACTGCCAGCCGGGCGGAGTGCCAGTCTGTGCCACCAATG 300


12716R-2.IR_full       301 GAACCGATAGCTTCTACTTCGAGAATCATTGCCGTTTGGAGGCGGCCAACATGAAGATGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12716R-2.in silico     301 GAACCGATAGCTTCTACTTCGAGAATCATTGCCGTTTGGAGGCGGCCAACATGAAGATGC 360


12716R-2.IR_full       361 TCTTCCAGCACGGCACAGAACTGGAGCCCACGGAGATGGAGCGATGCCTGCCCAACTGCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12716R-2.in silico     361 TCTTCCAGCACGGCACAGAACTGGAGCCCACGGAGATGGAGCGATGCCTGCCCAACTGCC 420


12716R-2.IR_full       421 AGACGATGAAGTGCACCCAGGTTGAGCGTCCAGTTTGCGCGCTGGCCGAGATTGGAGGAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12716R-2.in silico     421 AGACGATGAAGTGCACCCAGGTTGAGCGTCCAGTTTGCGCGCTGGCCGAGATTGGAGGAG 480


12716R-2.IR_full       481 CTATTCCACAAACATTCGCC 500
                           ||||||||||||||||||||
12716R-2.in silico     481 CTATTCCACAAACATTCGCC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_132617.1  CG12716-RA (CG12716), mRNA 
0.41   2  2  4  2  NM_139690.2  CG4618-RA (CG4618), mRNA 
0   0  2  4  5  NM_206764.1  CG4429-RC, transcript variant C (Rbp2), mRNA 
0   0  2  4  5  NM_080220.2  CG4429-RA, transcript variant A (Rbp2), mRNA 
0   0  2  4  5  NM_167522.2  CG4429-RB, transcript variant B (Rbp2), mRNA 
0   0  2  4  4  NM_139443.1  CG13809-RA (osm-1), mRNA 
0   0  2  2  6  NM_137081.2  CG30483-RA (Prosap), mRNA 
0   0  2  2  3  NM_168779.1  CG3979-RC, transcript variant C (Indy), mRNA 
0   0  2  2  3  NM_079426.2  CG3979-RA, transcript variant A (Indy), mRNA 
0   0  2  2  3  NM_168778.1  CG3979-RB, transcript variant B (Indy), mRNA 
0   0  2  2  3  NM_132346.1  CG3003-RB (CG3003), mRNA 
0   0  2  2  2  NM_142837.2  CG17141-RA (CG17141), mRNA 
0   0  2  2  2  NM_143783.2  CG5824-RA (l(3)07882), mRNA 
0   0  1  5  3  NM_167080.1  CG32742-RA (l(1)G0148), mRNA 
0   0  1  4  8  NM_057249.4  CG7793-RA (Sos), mRNA 
0   0  1  4  5  NM_164719.1  CG32829-RA (CG32829), mRNA 
0   0  1  4  2  NM_169452.1  CG31361-RB, transcript variant B (dpr17), mRNA 
0   0  1  4  2  NM_169451.1  CG31361-RA, transcript variant A (dpr17), mRNA 
0   0  1  3  4  NM_143093.2  CG13654-RA (CG13654), mRNA 
0   0  1  3  4  NM_136062.1  CG15167-RA (CG15167), mRNA 
0   0  1  3  3  NM_137713.2  CG4266-RA (CG4266), mRNA 
0   0  1  3  3  NM_140690.2  CG7729-RA (Fit2), mRNA 
0   0  1  3  2  NM_140307.2  CG10335-RA (Pbgs), mRNA 
0   0  1  2  5  NM_135766.2  CG16974-RA (CG16974), mRNA 
0   0  1  2  4  NM_132214.1  CG15335-RB (CG15335), mRNA 
0   0  1  2  3  NM_001038897.1  CG33971-RA (CG33971), mRNA 
0   0  1  2  3  NM_143930.1  CG8369-RA (CG8369), mRNA 
0   0  0  5  6  NM_057531.3  CG6863-RA, transcript variant A (tok), mRNA 
0   0  0  5  6  NM_164510.2  CG2991-RC, transcript variant C (CG2991), mRNA 
0   0  0  5  6  NM_170168.1  CG6863-RB, transcript variant B (tok), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.