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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12653R-1 
 Symbol btd  Full Name buttonhead 
 CG No CG12653  Old CG No CG12653 
 Synonyms BTD, CG12653, btd 
 Accession No (Link to NCBI) NM_078545.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGATCGATGCGGCCTGCAAT 
 Primer Seq. 3' 
aatctagaggtaccAATGACTGGGCAGGTGATGGG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGATCGATG CGGCCTGCAA TTACTTAAAT CCCTATGCGC AACAGCATCA GGCGCAGCAG 
0061 CAGCAACATG CGCAACATCA GCAACACGCG CAACAACAGC AACATCATCT GCACATGCAG 
0121 CAGGCGCAAC ATCATCTGCA CTTGTCGCAT CAGCAGGCGC AACAGCAGCA TATGCAACAC 
0181 TTGACACAGC AACAGCAGCA GCAGCAACAA CAACAGCAGC AACAGCAGCA GCAACAGCAG 
0241 CAACAACAAC AGCCGCAACA GCAGCAACAT GACTTCCTCA GCGCCGCCGC CCTGCTGTCC 
0301 GCCCCACCAT CCCTATCCGG CTCGAGTTCC GGCTCCAGCT CCGGCAGTTC TCCGCTATAT 
0361 GGAAAGCCGC CAATGAAGCT GGAACTGCCG TATCCACAGG CCTCATCCAC GGGCACCGCA 
0421 TCGCCCAACT CCAGCATACA ATCGGCGCCA TCATCGGCCT CCGTGTCGCC CAGCATCTTC 
0481 CCATCACCTG CCCAGTCATT  
 in silico PCR Fragment 
0001 ATGATCGATG CGGCCTGCAA TTACTTAAAT CCCTATGCGC AACAGCATCA GGCGCAGCAG 
0061 CAGCAACATG CGCAACATCA GCAACACGCG CAACAACAGC AACATCATCT GCACATGCAG 
0121 CAGGCGCAAC ATCATCTGCA CTTGTCGCAT CAGCAGGCGC AACAGCAGCA TATGCAACAC 
0181 TTGACACAGC AACAGCAGCA GCAGCAACAA CAACAGCAGC AACAGCAGCA GCAACAGCAG 
0241 CAACAACAAC AGCCGCAACA GCAGCAACAT GACTTCCTCA GCGCCGCCGC CCTGCTGTCC 
0301 GCCCCACCAT CCCTATCCGG CTCGAGTTCC GGCTCCAGCT CCGGCAGTTC TCCGCTATAT 
0361 GGAAAGCCGC CAATGAAGCT GGAACTGCCG TATCCACAGG CCTCATCCAC GGGCACCGCA 
0421 TCGCCCAACT CCAGCATACA ATCGGCGCCA TCATCGGCCT CCGTGTCGCC CAGCATCTTC 
0481 CCATCACCTG CCCAGTCATT  
 Assemble Data 

12653R-1.IR_full       1   ATGATCGATGCGGCCTGCAATTACTTAAATCCCTATGCGCAACAGCATCAGGCGCAGCAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12653R-1.in silico     1   ATGATCGATGCGGCCTGCAATTACTTAAATCCCTATGCGCAACAGCATCAGGCGCAGCAG 60


12653R-1.IR_full       61  CAGCAACATGCGCAACATCAGCAACACGCGCAACAACAGCAACATCATCTGCACATGCAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12653R-1.in silico     61  CAGCAACATGCGCAACATCAGCAACACGCGCAACAACAGCAACATCATCTGCACATGCAG 120


12653R-1.IR_full       121 CAGGCGCAACATCATCTGCACTTGTCGCATCAGCAGGCGCAACAGCAGCATATGCAACAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12653R-1.in silico     121 CAGGCGCAACATCATCTGCACTTGTCGCATCAGCAGGCGCAACAGCAGCATATGCAACAC 180


12653R-1.IR_full       181 TTGACACAGCAACAGCAGCAGCAGCAACAACAACAGCAGCAACAGCAGCAGCAACAGCAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12653R-1.in silico     181 TTGACACAGCAACAGCAGCAGCAGCAACAACAACAGCAGCAACAGCAGCAGCAACAGCAG 240


12653R-1.IR_full       241 CAACAACAACAGCCGCAACAGCAGCAACATGACTTCCTCAGCGCCGCCGCCCTGCTGTCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12653R-1.in silico     241 CAACAACAACAGCCGCAACAGCAGCAACATGACTTCCTCAGCGCCGCCGCCCTGCTGTCC 300


12653R-1.IR_full       301 GCCCCACCATCCCTATCCGGCTCGAGTTCCGGCTCCAGCTCCGGCAGTTCTCCGCTATAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12653R-1.in silico     301 GCCCCACCATCCCTATCCGGCTCGAGTTCCGGCTCCAGCTCCGGCAGTTCTCCGCTATAT 360


12653R-1.IR_full       361 GGAAAGCCGCCAATGAAGCTGGAACTGCCGTATCCACAGGCCTCATCCACGGGCACCGCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12653R-1.in silico     361 GGAAAGCCGCCAATGAAGCTGGAACTGCCGTATCCACAGGCCTCATCCACGGGCACCGCA 420


12653R-1.IR_full       421 TCGCCCAACTCCAGCATACAATCGGCGCCATCATCGGCCTCCGTGTCGCCCAGCATCTTC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12653R-1.in silico     421 TCGCCCAACTCCAGCATACAATCGGCGCCATCATCGGCCTCCGTGTCGCCCAGCATCTTC 480


12653R-1.IR_full       481 CCATCACCTGCCCAGTCATT 500
                           ||||||||||||||||||||
12653R-1.in silico     481 CCATCACCTGCCCAGTCATT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100.41  484  74  258  586  NM_078545.2  CG12653-RA (btd), mRNA 
25.51   123  1100  3062  6070  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
25.51   123  1100  3062  6070  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
25.51   123  1100  3062  6070  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
12.24   59  232  865  1867  NM_078797.2  CG13109-RA (tai), mRNA 
11.61   56  332  917  1875  NM_079903.2  CG15319-RB (nej), mRNA 
10.78   52  186  661  1657  NM_139799.3  CG10107-RA, transcript variant A (CG10107), mRNA 
10.78   52  186  661  1657  NM_206286.1  CG10107-RC, transcript variant C (CG10107), mRNA 
10.37   50  361  1040  2309  NM_135077.2  CG14023-RA (CG14023), mRNA 
10.16   49  201  494  812  NM_080487.2  CG10572-RA (Cdk8), mRNA 
9.95   48  242  727  1290  NM_167000.1  CG32778-RA (CG32778), mRNA 
9.75   47  427  1307  2886  NM_168571.2  CG32133-RA (CG32133), mRNA 
9.12   44  275  921  2162  NM_079671.2  CG7847-RA, transcript variant A (sr), mRNA 
8.5   41  371  1251  2745  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
8.5   41  371  1251  2745  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
8.29   40  178  543  1117  NM_001014576.1  CG7391-RD, transcript variant D (Clk), mRNA 
8.29   40  178  543  1117  NM_001014575.1  CG7391-RE, transcript variant E (Clk), mRNA 
8.29   40  178  543  1117  NM_001014574.1  CG7391-RF, transcript variant F (Clk), mRNA 
8.29   40  178  543  1117  NM_079240.2  CG7391-RA, transcript variant A (Clk), mRNA 
8.29   40  178  543  1117  NM_206299.1  CG7391-RC, transcript variant C (Clk), mRNA 
8.29   40  178  543  1117  NM_170628.1  CG7391-RB, transcript variant B (Clk), mRNA 
8.09   39  236  694  1298  NM_134474.4  CG32532-RA (CG32532), mRNA 
8.09   39  152  498  949  NM_206456.1  CG32466-RB, transcript variant B (rn), mRNA 
8.09   39  111  480  1130  NM_165037.1  CG6043-RC, transcript variant C (CG6043), mRNA 
8.09   39  111  480  1126  NM_165035.1  CG6043-RB, transcript variant B (CG6043), mRNA 
8.09   39  111  480  1126  NM_135785.1  CG6043-RD, transcript variant D (CG6043), mRNA 
8.09   39  111  480  1126  NM_165034.1  CG6043-RA, transcript variant A (CG6043), mRNA 
7.88   38  98  356  794  NM_169733.2  CG10325-RB, transcript variant B (abd-A), mRNA 
7.67   37  301  1052  2555  NM_001038734.1  CG16902-RC (Hr4), mRNA 
7.67   37  95  356  781  NM_057345.2  CG10325-RA, transcript variant A (abd-A), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
++ 
DB
++ 
DC
++ 
DM
+ 
VM
+ 
VB
+- 
Caspase-3 signal
N
++ 
DB
+ 
DC
+ 
DM
++ 
VM
++ 
VB
++ 
Dcompartment   Penetrance
++     

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