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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12652R-2 
 Symbol CG32700  Full Name CG32700 
 CG No CG32700  Old CG No CG12652 
 Synonyms CG2994, CG12652, CG12651, CG18429, BcDNA:GH19985, CG32700 
 Accession No (Link to NCBI) NM_167191.1 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCTCGTTCACGCCCGTCTTCAA 
 Primer Seq. 3' 
aatctagaggtaccATACACCGGAAATGGACTCGC 
 Predicted Fragment Size 
455 
 IR fragment full Seq 
0001 CTCGTTCACG CCCGTCTTCA ACTTGAGCAC CCAGGAGCCG CTGATCGTGG TGGAGGAATC 
0061 GCACACCGCC GAGGATGGTG ATGACTTGGA GGGTGCCGCC GGTGGATGTG ATCCCACTGC 
0121 GGCCGGGACA CGCCGTTCCA ACAGCGATGA CTCCGAACCG GACTCGCCGG TGAATCCCTA 
0181 TCTGCTTTGC CCATTTCCCG ATATGCAACA GCGACGCAAG CATTCGCTGC CATCGCTTCA 
0241 AATTACCGAG GGCATCACCG CCAGTCAGGT GCGTCGATTG TCGGAGGTGG GCGGCGAGAC 
0301 TGGCGGGCTC AGTCCCAAGG AGGTCGAGTT TCTGGCCACG CTCTCGCAGA AGGCCAATCC 
0361 GACGGCCGGT GGACGTAGGC ACTCGGTGGT CACCATATCG GCGGTGCCGC CAACGCTTTT 
0421 CGGACGCAAT CGCCGCGAGT CCATTTCCGG TGTAT 
 in silico PCR Fragment 
0001 CTCGTTCACG CCCGTCTTCA ACTTGAGCAC CCAGGAGCCG CTGATCGTGG TGGAGGAATC 
0061 GCACACCGCC GAGGATGGTG ATGACTTGGA GGGTGCCGCC GGTGGATGTG ATCCCACTGC 
0121 GGCCGGGACA CGCCGTTCCA ACAGCGATGA CTCCGAACCG GACTCGCCGG TGAATCCCTA 
0181 TCTGCTTTGC CCATTTCCCG ATATGCAACA GCGACGCAAG CATTCGCTGC CATCGCTTCA 
0241 AATTACCGAG GGCATCACCG CCAGTCAGGT GCGTCGATTG TCGGAGGTGG GCGGCGAGAC 
0301 TGGCGGGCTC AGTCCCAAGG AGGTCGAGTT TCTGGCCACG CTCTCGCAGA AGGCCAATCC 
0361 GACGGCCGGT GGACGTAGGC ACTCGGTGGT CACCATATCG GCGGTGCCGC CAACGCTTTT 
0421 CGGACGCAAT CGCCGCGAGT CCATTTCCGG TGTAT 
 Assemble Data 

12652R-2.IR_full       1   CTCGTTCACGCCCGTCTTCAACTTGAGCACCCAGGAGCCGCTGATCGTGGTGGAGGAATC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12652R-2.in silico     1   CTCGTTCACGCCCGTCTTCAACTTGAGCACCCAGGAGCCGCTGATCGTGGTGGAGGAATC 60


12652R-2.IR_full       61  GCACACCGCCGAGGATGGTGATGACTTGGAGGGTGCCGCCGGTGGATGTGATCCCACTGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12652R-2.in silico     61  GCACACCGCCGAGGATGGTGATGACTTGGAGGGTGCCGCCGGTGGATGTGATCCCACTGC 120


12652R-2.IR_full       121 GGCCGGGACACGCCGTTCCAACAGCGATGACTCCGAACCGGACTCGCCGGTGAATCCCTA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12652R-2.in silico     121 GGCCGGGACACGCCGTTCCAACAGCGATGACTCCGAACCGGACTCGCCGGTGAATCCCTA 180


12652R-2.IR_full       181 TCTGCTTTGCCCATTTCCCGATATGCAACAGCGACGCAAGCATTCGCTGCCATCGCTTCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12652R-2.in silico     181 TCTGCTTTGCCCATTTCCCGATATGCAACAGCGACGCAAGCATTCGCTGCCATCGCTTCA 240


12652R-2.IR_full       241 AATTACCGAGGGCATCACCGCCAGTCAGGTGCGTCGATTGTCGGAGGTGGGCGGCGAGAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12652R-2.in silico     241 AATTACCGAGGGCATCACCGCCAGTCAGGTGCGTCGATTGTCGGAGGTGGGCGGCGAGAC 300


12652R-2.IR_full       301 TGGCGGGCTCAGTCCCAAGGAGGTCGAGTTTCTGGCCACGCTCTCGCAGAAGGCCAATCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12652R-2.in silico     301 TGGCGGGCTCAGTCCCAAGGAGGTCGAGTTTCTGGCCACGCTCTCGCAGAAGGCCAATCC 360


12652R-2.IR_full       361 GACGGCCGGTGGACGTAGGCACTCGGTGGTCACCATATCGGCGGTGCCGCCAACGCTTTT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12652R-2.in silico     361 GACGGCCGGTGGACGTAGGCACTCGGTGGTCACCATATCGGCGGTGCCGCCAACGCTTTT 420


12652R-2.IR_full       421 CGGACGCAATCGCCGCGAGTCCATTTCCGGTGTAT 455
                           |||||||||||||||||||||||||||||||||||
12652R-2.in silico     421 CGGACGCAATCGCCGCGAGTCCATTTCCGGTGTAT 455



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   437  0  0  0  NM_167192.1  CG32700-RB, transcript variant B (CG32700), mRNA 
100   437  0  0  0  NM_167191.1  CG32700-RA, transcript variant A (CG32700), mRNA 
0   0  8  28  31  NM_133058.2  CG6123-RA (CG6123), mRNA 
0   0  3  4  2  NM_132792.2  CG6227-RA (CG6227), mRNA 
0   0  2  5  2  NM_080052.3  CG18350-RC, transcript variant C (Sxl), mRNA 
0   0  2  5  2  NM_167118.2  CG18350-RB, transcript variant B (Sxl), mRNA 
0   0  2  5  2  NM_001031894.1  CG18350-RL, transcript variant L (Sxl), mRNA 
0   0  2  5  2  NM_167111.2  CG18350-RI, transcript variant I (Sxl), mRNA 
0   0  2  5  2  NM_001031895.1  CG18350-RK, transcript variant K (Sxl), mRNA 
0   0  2  5  2  NM_167110.2  CG18350-RJ, transcript variant J (Sxl), mRNA 
0   0  2  5  2  NM_001031892.1  CG18350-RN, transcript variant N (Sxl), mRNA 
0   0  2  5  2  NM_001031893.1  CG18350-RM, transcript variant M (Sxl), mRNA 
0   0  2  5  2  NM_167113.2  CG18350-RE, transcript variant E (Sxl), mRNA 
0   0  2  5  2  NM_167114.2  CG18350-RH, transcript variant H (Sxl), mRNA 
0   0  2  5  2  NM_167115.2  CG18350-RA, transcript variant A (Sxl), mRNA 
0   0  2  5  2  NM_167116.2  CG18350-RG, transcript variant G (Sxl), mRNA 
0   0  2  5  2  NM_167117.2  CG18350-RF, transcript variant F (Sxl), mRNA 
0   0  2  5  2  NM_167112.2  CG18350-RD, transcript variant D (Sxl), mRNA 
0   0  2  5  2  NM_001031891.1  CG18350-RO, transcript variant O (Sxl), mRNA 
0   0  2  5  2  NM_001031890.1  CG14425-RA, transcript variant A (CG14425), mRNA 
0   0  1  7  2  NM_142396.1  CG7397-RA (CG7397), mRNA 
0   0  1  3  5  NM_135077.2  CG14023-RA (CG14023), mRNA 
0   0  1  3  2  NM_136175.2  CG10728-RA (vls), mRNA 
0   0  1  2  6  NM_135086.2  CG11024-RA (cl), mRNA 
0   0  1  2  3  NM_135010.1  CG11928-RA (CG11928), mRNA 
0   0  1  2  2  NM_079554.2  CG7954-RA, transcript variant A (stck), mRNA 
0   0  1  2  2  NM_142883.1  CG4434-RA (CG4434), mRNA 
0   0  1  2  2  NM_169220.1  CG7954-RB, transcript variant B (stck), mRNA 
0   0  1  2  2  NM_169221.1  CG7954-RC, transcript variant C (stck), mRNA 
0   0  0  5  3  NM_001014507.1  CG8732-RI, transcript variant I (l(2)44DEa), mRNA 

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