National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12534R-1 
 Symbol CG12534  Full Name CG12534 
 CG No CG12534  Old CG No CG12534 
 Synonyms CG12534 
 Accession No (Link to NCBI) NM_134509.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACAACTACGGCTACGACTACA 
 Primer Seq. 3' 
aatctagaggtaccGCGCGACAGAACCTCAAAAAA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACAACTACGG CTACGACTAC ATCAGCCCCA CGCACATCGA ACTCAGCCGG GAAGCAACTC 
0061 GCCTGGTAAT TCGCCGTCTG CAGCgNACCG TTCTCCGCCT GATCCGTGAC CCGGACTTCA 
0121 TCATGTCTGC CGAGGCGAAT CCCGAGATGG ATAAGGAGCA CCACGAGTCC CCATTCGCAG 
0181 CGAACCGCAA GGCCGGTGCC AGCGGCGCCG GCAAACAGGN ACTCGAACTG CCGCACCTGC 
0241 AACGATTTCA AGTCGTGGTC CAAGCAGCAA CGCCTCATCT CCAACGTGCA GAGCGCCAAG 
0301 GTGAAGGCAC ATGGCCGCCG CCGAAAAGCT GACCGTCAAC GCCGCCGAAG ATCCGCTGCC 
0361 CCGCGACGAC TGCCCGCTGG ACAAGGTGAG GCTGGGAATT TCCACCTGGG GACTGCTCCA 
0421 CACCATGGCC GCCTTTTACT CGGACAACCC CACGGACACC GAGAAGCGCG ACATGAAGAC 
0481 CTTTTTTGAG GTTCTTGTCG CGC 
 in silico PCR Fragment 
0001 ACAACTACGG CTACGACTAC ATCAGCCCCA CGCACATCGA ACTCAGCCGG GAAGCAACTC 
0061 GCCTGGTAAT TCGCCGTCTG CAGCGCACCG TTCTCCGCCT GATCCGTGAC CCGGACTTCA 
0121 TCATGTCTGC CGAGGCGAAT CCCGAGATGG ATAAGGAGCA CCACGAGTCC CCATTCGCAG 
0181 CGAACCGCAA GGCCGGTGCC AGCGGCGCCG GCAAACAGG- ACTCGAACTG CCGCACCTGC 
0241 AACGATTTCA AGTCGTGGTC CAAGCAGCAA CGCCTCATCT CCAACGTGCA GAGCGCCAAG 
0301 GTGAA-GCAC ATGGCCGCCG CCGAAAAGCT GACCGTCAAC GCCGCCGAAG ATCCGCTGCC 
0361 CCGCGACGAC TGCCCGCTGG ACAAGGTGAG GCTGGGAATT TCCACCTGGG GACTGCTCCA 
0421 CACCATGGCC GCCTTTTACT CGGACAACCC CACGGACACC GAGAAGCGCG ACATGAAGAC 
0481 CTTTTTTGAG GTTC-TGTCG CGC 
 Assemble Data 

12534R-1.IR_full       1   ACAACTACGGCTACGACTACATCAGCCCCACGCACATCGAACTCAGCCGGGAAGCAACTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12534R-1.in silico     1   ACAACTACGGCTACGACTACATCAGCCCCACGCACATCGAACTCAGCCGGGAAGCAACTC 60


12534R-1.IR_full       61  GCCTGGTAATTCGCCGTCTGCAGCGNACCGTTCTCCGCCTGATCCGTGACCCGGACTTCA 120
                           ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
12534R-1.in silico     61  GCCTGGTAATTCGCCGTCTGCAGCGCACCGTTCTCCGCCTGATCCGTGACCCGGACTTCA 120


12534R-1.IR_full       121 TCATGTCTGCCGAGGCGAATCCCGAGATGGATAAGGAGCACCACGAGTCCCCATTCGCAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12534R-1.in silico     121 TCATGTCTGCCGAGGCGAATCCCGAGATGGATAAGGAGCACCACGAGTCCCCATTCGCAG 180


12534R-1.IR_full       181 CGAACCGCAAGGCCGGTGCCAGCGGCGCCGGCAAACAGGNACTCGAACTGCCGCACCTGC 240
                           ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
12534R-1.in silico     181 CGAACCGCAAGGCCGGTGCCAGCGGCGCCGGCAAACAGG-ACTCGAACTGCCGCACCTGC 240


12534R-1.IR_full       241 AACGATTTCAAGTCGTGGTCCAAGCAGCAACGCCTCATCTCCAACGTGCAGAGCGCCAAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12534R-1.in silico     241 AACGATTTCAAGTCGTGGTCCAAGCAGCAACGCCTCATCTCCAACGTGCAGAGCGCCAAG 300


12534R-1.IR_full       301 GTGAAGGCACATGGCCGCCGCCGAAAAGCTGACCGTCAACGCCGCCGAAGATCCGCTGCC 360
                           ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
12534R-1.in silico     301 GTGAA-GCACATGGCCGCCGCCGAAAAGCTGACCGTCAACGCCGCCGAAGATCCGCTGCC 360


12534R-1.IR_full       361 CCGCGACGACTGCCCGCTGGACAAGGTGAGGCTGGGAATTTCCACCTGGGGACTGCTCCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12534R-1.in silico     361 CCGCGACGACTGCCCGCTGGACAAGGTGAGGCTGGGAATTTCCACCTGGGGACTGCTCCA 420


12534R-1.IR_full       421 CACCATGGCCGCCTTTTACTCGGACAACCCCACGGACACCGAGAAGCGCGACATGAAGAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12534R-1.in silico     421 CACCATGGCCGCCTTTTACTCGGACAACCCCACGGACACCGAGAAGCGCGACATGAAGAC 480


12534R-1.IR_full       481 CTTTTTTGAGGTTCTTGTCGCGC 503
                           |||||||||||||| ||||||||
12534R-1.in silico     481 CTTTTTTGAGGTTC-TGTCGCGC 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_134509.1  CG12534-RA (CG12534), mRNA 
0   0  4  4  7  NM_057798.2  CG1650-RA (unpg), mRNA 
0   0  3  2  6  NM_057295.3  CG4696-RA, transcript variant A (Mp20), mRNA 
0   0  3  2  6  NM_001014522.1  CG4696-RB, transcript variant B (Mp20), mRNA 
0   0  3  1  5  NM_079938.3  CG2102-RA (cas), mRNA 
0   0  2  5  4  NM_167309.1  CG32663-RA (CG32663), mRNA 
0   0  2  5  3  NM_141115.1  CG14562-RA (CG14562), mRNA 
0   0  2  4  2  NM_141935.2  CG5663-RA (Dip-C), mRNA 
0   0  2  4  2  NM_142786.1  CG13847-RA (CG13847), mRNA 
0   0  2  3  6  NM_168126.1  CG5406-RB, transcript variant B (sif), mRNA 
0   0  2  2  2  NM_206278.1  CG5406-RC, transcript variant C (sif), mRNA 
0   0  2  2  2  NM_079908.2  CG5406-RA, transcript variant A (sif), mRNA 
0   0  1  4  5  NM_132919.2  CG9699-RC, transcript variant C (CG9699), mRNA 
0   0  1  4  5  NM_167531.1  CG9699-RB, transcript variant B (CG9699), mRNA 
0   0  1  4  5  NM_167533.1  CG9699-RE, transcript variant E (CG9699), mRNA 
0   0  1  4  5  NM_167532.1  CG9699-RD, transcript variant D (CG9699), mRNA 
0   0  1  4  5  NM_167534.1  CG9699-RF, transcript variant F (CG9699), mRNA 
0   0  1  4  5  NM_167530.1  CG9699-RA, transcript variant A (CG9699), mRNA 
0   0  1  3  10  NM_164457.1  CG3166-RA, transcript variant A (aop), mRNA 
0   0  1  3  7  NM_140532.2  CG7372-RA (CG7372), mRNA 
0   0  1  3  5  NM_134829.2  CG4272-RA, transcript variant A (CG4272), mRNA 
0   0  1  3  5  NM_164480.1  CG4272-RB, transcript variant B (CG4272), mRNA 
0   0  1  3  5  NM_132267.4  CG12109-RB (Caf1-180), mRNA 
0   0  1  3  5  NM_142644.1  CG5466-RA (CG5466), mRNA 
0   0  1  2  5  NM_001042904.1  CG15148-RB, transcript variant B (btv), mRNA 
0   0  1  2  5  NM_001042905.1  CG15148-RC, transcript variant C (btv), mRNA 
0   0  1  2  5  NM_164503.1  CG16987-RB, transcript variant B (Alp23B), mRNA 
0   0  1  2  5  NM_078737.2  CG16987-RA, transcript variant A (Alp23B), mRNA 
0   0  1  2  5  NM_142691.1  CG5798-RA (CG5798), mRNA 
0   0  1  2  4  NM_164966.2  CG6464-RA (salm), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.