NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 12491R-3

Symbol CG12491 Full Name CG12491

CG No CG12491 Old CG No CG12491

Synonyms CG12491

Accession No (Link to NCBI) NM_137980.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCCGAATTCGTCCTGGCCTTT

Primer Seq. 3'

aatctagaggtaccACGTTGGCGCCTTCTTCCAGC

Predicted Fragment Size

IR fragment full Seq

0001 CCCGAATTCG TCCTGGCCTT TGGTTTGGTT GTCCTCGTGG CGACCGTTTA TGGTGGCACC 
0061 GACTCATCCT CGAGCGACTC GTCCTCTTCC ACATCACCCA CTTCCAGTTC ATCGACTCCA 
0121 TCGACTTCCA GCTCAAGTTC GACACCCAGC TCTTCGTCCT CCACCTCAAC TCCGTCCAGC 
0181 AACAGCACNT ACGTCTACTT CGTCCTCCAC TCCTAGCAGC TCTTCTTCCA CTTCGCCCAC 
0241 TTCCTCCACC TCATCGACGA CTGCCACAAC CACGGCCCCC TCCACGTCCA GCGACACGTC 
0301 CTCCTCCTCC TCGAGCTCCG ACAGCGAGGA GGTGGACAGG CTGAGGCGCA GGCTTCGCAG 
0361 GCTCCGGCGG CTGAGGCGTC AGGAGAGGCG CCAGGAGATT CGCCGGGAGA GGCAGCAGGA 
0421 AAGGCGCCAG CAGAGTCGTG CTGGAAGAAg GCGCCAACGT

in silico PCR Fragment

0001 CCCGAATTCG TCCTGGCCTT TGGTTTGGTT GTCCTCGTGG CGACCGTTTA TGGTGGCACC 
0061 GACTCATCCT CGAGCGACTC GTCCTCTTCC ACATCACCCA CTTCCAATTC ATCGACTCCA 
0121 TCGACTTCCA GCTCAAGTTC GACACCCAGC TCTTCGTCCT CCACCTCAAC TCCGTCCAGC 
0181 AATAGCAC-T ACGTCTACTT CGTCCTCCAC TCCTAGCAGC TCTTCTTCCA CTTCGCCCAC 
0241 TTCCTCCACC TCATCGACGA CTGCCACAAC CACGGCCCCC TCCACGTCCA GCGACACGTC 
0301 CTCCTCCTCC ACGAGCTCCG ACAGCGAGGA GGTGGACAGG CTGAGGCGCA GGCTTCGCAG 
0361 GCTCCGGCGG CTGAGGCGTC AGGAGAGGCG CCAGGAGATT CGCCGGGAGA GGCAGCAGGA 
0421 AAGGCGCCAG CAGAGTCGTG CTGGAAGAAG GCGCCAACGT

Assemble Data


12491R-3.IR_full       1   CCCGAATTCGTCCTGGCCTTTGGTTTGGTTGTCCTCGTGGCGACCGTTTATGGTGGCACC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12491R-3.in silico     1   CCCGAATTCGTCCTGGCCTTTGGTTTGGTTGTCCTCGTGGCGACCGTTTATGGTGGCACC 60


12491R-3.IR_full       61  GACTCATCCTCGAGCGACTCGTCCTCTTCCACATCACCCACTTCCAGTTCATCGACTCCA 120
                           |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
12491R-3.in silico     61  GACTCATCCTCGAGCGACTCGTCCTCTTCCACATCACCCACTTCCAATTCATCGACTCCA 120


12491R-3.IR_full       121 TCGACTTCCAGCTCAAGTTCGACACCCAGCTCTTCGTCCTCCACCTCAACTCCGTCCAGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12491R-3.in silico     121 TCGACTTCCAGCTCAAGTTCGACACCCAGCTCTTCGTCCTCCACCTCAACTCCGTCCAGC 180


12491R-3.IR_full       181 AACAGCACNTACGTCTACTTCGTCCTCCACTCCTAGCAGCTCTTCTTCCACTTCGCCCAC 240
                           || ||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
12491R-3.in silico     181 AATAGCAC-TACGTCTACTTCGTCCTCCACTCCTAGCAGCTCTTCTTCCACTTCGCCCAC 240


12491R-3.IR_full       241 TTCCTCCACCTCATCGACGACTGCCACAACCACGGCCCCCTCCACGTCCAGCGACACGTC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12491R-3.in silico     241 TTCCTCCACCTCATCGACGACTGCCACAACCACGGCCCCCTCCACGTCCAGCGACACGTC 300


12491R-3.IR_full       301 CTCCTCCTCCTCGAGCTCCGACAGCGAGGAGGTGGACAGGCTGAGGCGCAGGCTTCGCAG 360
                           |||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
12491R-3.in silico     301 CTCCTCCTCCACGAGCTCCGACAGCGAGGAGGTGGACAGGCTGAGGCGCAGGCTTCGCAG 360


12491R-3.IR_full       361 GCTCCGGCGGCTGAGGCGTCAGGAGAGGCGCCAGGAGATTCGCCGGGAGAGGCAGCAGGA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12491R-3.in silico     361 GCTCCGGCGGCTGAGGCGTCAGGAGAGGCGCCAGGAGATTCGCCGGGAGAGGCAGCAGGA 420


12491R-3.IR_full       421 AAGGCGCCAGCAGAGTCGTGCTGGAAGAAGGCGCCAACGT 460
                           ||||||||||||||||||||||||||||||||||||||||
12491R-3.in silico     421 AAGGCGCCAGCAGAGTCGTGCTGGAAGAAGGCGCCAACGT 460

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	441	0	8	44	NM_001043102.1	CG34105-RA (CG34105), mRNA
100	441	0	8	44	NM_137980.2	CG12491-RA (CG12491), mRNA
0.45	2	4	3	33	NM_137979.2	CG13560-RA (CG13560), mRNA
0.45	2	2	4	11	NM_168441.1	CG32071-RA (CG32071), mRNA
0.22	1	4	2	4	NM_078683.2	CG7103-RA (Pvf1), mRNA
0	0	3	2	9	NM_140681.3	CG9665-RA (CG9665), mRNA
0	0	2	14	17	NM_169134.1	CG31556-RA (CG31556), mRNA
0	0	2	6	9	NM_135661.2	CG6509-RB, transcript variant B (CG6509), mRNA
0	0	2	6	9	NM_164967.1	CG6509-RA, transcript variant A (CG6509), mRNA
0	0	2	5	3	NM_136028.2	CG7180-RA (CG7180), mRNA
0	0	2	4	6	NM_167857.1	CG1200-RA, transcript variant A (Aplip1), mRNA
0	0	2	4	6	NM_167856.1	CG1200-RB, transcript variant B (Aplip1), mRNA
0	0	2	4	2	NM_136627.1	CG13740-RA (CG13740), mRNA
0	0	2	3	8	NM_057519.3	CG17998-RA (Gprk2), mRNA
0	0	2	3	4	NM_137034.2	CG17064-RA (mars), mRNA
0	0	2	2	2	NM_169590.1	CG14853-RA, transcript variant A (CG14853), mRNA
0	0	2	2	2	NM_142119.2	CG14853-RB, transcript variant B (CG14853), mRNA
0	0	1	7	11	NM_143328.1	CG12875-RA (CG12875), mRNA
0	0	1	6	13	NM_058132.2	CG11886-RA (Slbp), mRNA
0	0	1	5	8	NM_137723.1	CG15660-RA (CG15660), mRNA
0	0	1	5	3	NM_169750.2	CG31266-RA (CG31266), mRNA
0	0	1	5	3	NM_143508.1	CG7928-RA (CG7928), mRNA
0	0	1	4	11	NM_001014545.1	CG33519-RB (Unc-89), mRNA
0	0	1	4	2	NM_142832.1	CG6982-RA (CG6982), mRNA
0	0	1	4	2	NM_079606.2	CG6819-RA (mbo), mRNA
0	0	1	4	2	NM_143365.2	CG10009-RA (Noa36), mRNA
0	0	1	3	11	NM_001014463.1	CG3234-RE, transcript variant E (tim), mRNA
0	0	1	3	11	NM_001038783.1	CG3234-RF, transcript variant F (tim), mRNA
0	0	1	3	11	NM_164540.2	CG3234-RD, transcript variant D (tim), mRNA
0	0	1	3	11	NM_164542.3	CG3234-RB, transcript variant B (tim), mRNA

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