NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 12484R-1

Symbol CG12484 Full Name CG12484

CG No CG12484 Old CG No CG12484

Synonyms CG12484, CG33041, CT32777, CG30143, FBgn0050143

Accession No (Link to NCBI) NM_176234.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92 [ PubMed ID = 19363474 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAGCTCCTCGACCACCATGAC

Primer Seq. 3'

aatctagaggtaccCAGAGCCCTCGTTGTAAGGAC

Predicted Fragment Size

IR fragment full Seq

0001 CAGCTCCTCG ACCACCATGA CCAGGAACAT CGAGGAGGAG AATGCCCTAC TTTTACTTTT 
0061 AGCTGGACCT CCCGTGCTAT CTGAGTCCAT AATGGGTACG GTCGGCCGGC TGCCCTGCAA 
0121 CGTCACCCCG CCCATTTACG AGGATCGAGT GGCGCTGGTT ATCTGGTACA AAGTTGGCCT 
0181 GAAGACTCCC ATTTACAGTG TGGACACACG CGACTCGAAC TTCGCCCAGG GAACGCACTG 
0241 GTCGGATGAG ACCTACCGGG AGCGGCTCTC CTTCCACGTG GAAGGGACGT GCTGGCACGC 
0301 TGACCATCAA GTCAACCACG GAGGACGACA CGGGCGANGT ATCgNTGCCG CGTCGACTTC 
0361 CAGAAGAGTC CGACTCGCAA CTCCAAAGTG AATTTAACTG TCATAATTCC GCCCGAGTCG 
0421 GTGATTATAT TGGACAGCAA GGGTGTGACC ATCGAGGATC ACACGCTGGG TCCTTACAAC 
0481 GAGGGCTCTG

in silico PCR Fragment

0001 CAGCTCCTCG ACCACCATGA CCAGGAACAT CGAGGAGGAG AATGCCCTAC TTTTACTTTT 
0061 AGCTGGACCT CCCGTGCTAT CTGAGTCCAT AATGGGTACG GTCGGCCGGC TGCCCTGCAA 
0121 CGTCACCCCG CCCATTTACG AGGATCGAGT GGCGCTGGTT ATCTGGTACA AAGTTGGCCT 
0181 GAAGACTCCC ATTTACAGTG TGGACACACG CGACTCGAAC TTCGCCCAGG GAACGCACTG 
0241 GTCGGATGAG ACCTACCGGG AGCGGCTCTC CTTCCACGTG GAA-GGACGT GCTGGCACGC 
0301 TGACCATCAA GTCAACCACG GAGGACGACA CGGGCGA-GT ATCGCTGCCG CGTCGACTTC 
0361 CAGAAGAGTC CGACTCGCAA CTCCAAAGTG AATTTAACTG TCATAATTCC GCCCGAGTCG 
0421 GTGATTATAT TGGACAGCAA GGGTGTGACC ATCGAGGATC ACACGCTGGG TCCTTACAAC 
0481 GAGGGCTCTG

Assemble Data


12484R-1.IR_full       1   CAGCTCCTCGACCACCATGACCAGGAACATCGAGGAGGAGAATGCCCTACTTTTACTTTT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12484R-1.in silico     1   CAGCTCCTCGACCACCATGACCAGGAACATCGAGGAGGAGAATGCCCTACTTTTACTTTT 60


12484R-1.IR_full       61  AGCTGGACCTCCCGTGCTATCTGAGTCCATAATGGGTACGGTCGGCCGGCTGCCCTGCAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12484R-1.in silico     61  AGCTGGACCTCCCGTGCTATCTGAGTCCATAATGGGTACGGTCGGCCGGCTGCCCTGCAA 120


12484R-1.IR_full       121 CGTCACCCCGCCCATTTACGAGGATCGAGTGGCGCTGGTTATCTGGTACAAAGTTGGCCT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12484R-1.in silico     121 CGTCACCCCGCCCATTTACGAGGATCGAGTGGCGCTGGTTATCTGGTACAAAGTTGGCCT 180


12484R-1.IR_full       181 GAAGACTCCCATTTACAGTGTGGACACACGCGACTCGAACTTCGCCCAGGGAACGCACTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12484R-1.in silico     181 GAAGACTCCCATTTACAGTGTGGACACACGCGACTCGAACTTCGCCCAGGGAACGCACTG 240


12484R-1.IR_full       241 GTCGGATGAGACCTACCGGGAGCGGCTCTCCTTCCACGTGGAAGGGACGTGCTGGCACGC 300
                           ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
12484R-1.in silico     241 GTCGGATGAGACCTACCGGGAGCGGCTCTCCTTCCACGTGGAA-GGACGTGCTGGCACGC 300


12484R-1.IR_full       301 TGACCATCAAGTCAACCACGGAGGACGACACGGGCGANGTATCGNTGCCGCGTCGACTTC 360
                           ||||||||||||||||||||||||||||||||||||| |||||| |||||||||||||||
12484R-1.in silico     301 TGACCATCAAGTCAACCACGGAGGACGACACGGGCGA-GTATCGCTGCCGCGTCGACTTC 360


12484R-1.IR_full       361 CAGAAGAGTCCGACTCGCAACTCCAAAGTGAATTTAACTGTCATAATTCCGCCCGAGTCG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12484R-1.in silico     361 CAGAAGAGTCCGACTCGCAACTCCAAAGTGAATTTAACTGTCATAATTCCGCCCGAGTCG 420


12484R-1.IR_full       421 GTGATTATATTGGACAGCAAGGGTGTGACCATCGAGGATCACACGCTGGGTCCTTACAAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12484R-1.in silico     421 GTGATTATATTGGACAGCAAGGGTGTGACCATCGAGGATCACACGCTGGGTCCTTACAAC 480


12484R-1.IR_full       481 GAGGGCTCTG 490
                           ||||||||||
12484R-1.in silico     481 GAGGGCTCTG 490

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	470	2	2	2	NM_176234.1	CG33041-RA (CG33041), mRNA
0	0	7	3	8	NM_001043216.1	CG34113-RO, transcript variant O (CG34113), mRNA
0	0	7	3	7	NM_001043217.1	CG34113-RP, transcript variant P (CG34113), mRNA
0	0	5	4	3	NM_134847.2	CG9883-RA (CG9883), mRNA
0	0	3	2	2	NM_136537.1	CG8693-RA (CG8693), mRNA
0	0	2	2	6	NM_166546.2	CG30092-RD, transcript variant D (jbug), mRNA
0	0	2	2	3	NM_132869.2	CG9172-RA, transcript variant A (CG9172), mRNA
0	0	2	2	3	NM_167476.1	CG9172-RB, transcript variant B (CG9172), mRNA
0	0	2	2	2	NM_134506.1	CG12531-RA (CG12531), mRNA
0	0	1	4	3	NM_140319.1	CG10616-RA (CG10616), mRNA
0	0	1	3	12	NM_132335.2	CG12139-RB (CG12139), mRNA
0	0	1	2	4	NM_165147.1	CG4894-RD, transcript variant D (Ca-alpha1D), mRNA
0	0	1	2	4	NM_134429.2	CG4894-RA, transcript variant A (Ca-alpha1D), mRNA
0	0	1	2	4	NM_165146.1	CG4894-RC, transcript variant C (Ca-alpha1D), mRNA
0	0	1	2	4	NM_080365.2	CG4894-RB, transcript variant B (Ca-alpha1D), mRNA
0	0	1	2	3	NM_169877.1	CG4662-RB, transcript variant B (CG4662), mRNA
0	0	0	6	2	NM_164688.1	CG31640-RA (CG31640), mRNA
0	0	0	6	2	NM_135118.1	CG14004-RA (CG14004), mRNA
0	0	0	5	4	NM_057449.3	CG5363-RA (cdc2), mRNA
0	0	0	5	4	NM_144172.1	CG8750-RA (CG8750), mRNA
0	0	0	5	3	NM_135992.2	CG6840-RA (Rpb11), mRNA
0	0	0	5	3	NM_139362.2	CG12091-RA (CG12091), mRNA
0	0	0	4	6	NM_001031964.1	CG13702-RC, transcript variant C (AlCR2), mRNA
0	0	0	4	6	NM_140782.4	CG13702-RB, transcript variant B (AlCR2), mRNA
0	0	0	4	5	NM_141137.1	CG11426-RA (CG11426), mRNA
0	0	0	4	4	NM_001038963.1	CG14372-RB (CG14372), mRNA
0	0	0	4	3	NM_135733.1	CG15485-RA (CG15485), mRNA
0	0	0	4	2	NM_001014568.1	CG33523-RB, transcript variant B (CG33523), mRNA
0	0	0	4	2	NM_001014567.1	CG33523-RC, transcript variant C (CG33523), mRNA
0	0	0	4	2	NM_001014566.1	CG33523-RA, transcript variant A (CG33523), mRNA

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