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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1245R-2 |
| Symbol |
MED27 |
Full Name |
Mediator complex subunit 27 |
| CG No |
CG1245 |
Old CG No |
CG1245 |
| Synonyms |
Med27, Trap37, CRSP34, dCRSP34, dTRAP37, CG1245, MED27 |
| Accession No (Link to NCBI) |
NM_141312.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCAAATACGTGAGGTAGAGCA |
| Primer Seq. 3' |
aatctagaggtaccGGAACACCTCGTAACGTGACT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCAAATACGT GAGGTAGAGC AGCTGATTAA TGGACTGCCG GTGCCACCAA CACCCTATTC
0061 TCTCGGGAAC ACCGCCTACC TGGCGCAGGA AACTTCGCAG GATCGACAGG CTCTATACCC
0121 TCAGCTGGTG AACAGCTACA AGTGGATGGA CAAGGTGCAC GACCACAGCT TCTTGGCCTT
0181 CAACAATCTC AACCAGAACA CACTCAGGCG CTCCTACAAT TACTGCTCGC AGAAAGCGCC
0241 AGCGACTGCC CTTCTCGTCC TTCAACAACG ATCCAGACCA CATAGATAAG CTCCTGAGTG
0301 AGATCAACAC CCCTCCGCAT ACTTCGTACA GAATTTTTCG TCCCTTCGGC ACAAACGCTG
0361 TCACCATCGT AACAATAAGT AACGTGATGA AGGCGGCTAT TGTCTTCAAA GGAGTGCTTA
0421 TAGAGTGGGT TACGGTTAAG GGATTCGATG AGCCGCTGGA GCATGACGAC CTGTGGGCCG
0481 AGTCACGTTA CGAGGTGTTC C |
| in silico PCR Fragment |
0001 GCAAATACGT GAGGTAGAGC AGCTGATTAA TGGACTGCCG GTGCCACCAA CACCCTATTC
0061 TCTCGGGAAC ACCGCCTACC TGGCGCAGGA AACTTCGCAG GATCGACAGG CTCTATACCC
0121 TCAGCTGGTG AACAGCTACA AGTGGATGGA CAAGGTGCAC GACCACAGCT TCTTGGCCTT
0181 CAACAATCTC AACCAGAACA CACTCAGGCG CTCCTACAAT TACTGCTCGC AG-AAGCGCC
0241 AGCGACTGCC CTTCTCGTCC TTCAACAACG ATCCAGACCA CATAGATAAG CTCCTGAGTG
0301 AGATCAACAC CCCTCCGCAT ACTTCGTACA GAATTTTTCG TCCCTTCGGC ACAAACGCTG
0361 TCACCATCGT AACAATAAGT AACGTGATGA AGGCGGCTAT TGTCTTCAAA GGAGTGCTTA
0421 TAGAGTGGGT TACGGTTAAG GGATTCGATG AGCCGCTGGA GCATGACGAC CTGTGGGCCG
0481 AGTCACGTTA CGAGGTGTTC C |
| Assemble Data |
1245R-2.IR_full 1 GCAAATACGTGAGGTAGAGCAGCTGATTAATGGACTGCCGGTGCCACCAACACCCTATTC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1245R-2.in silico 1 GCAAATACGTGAGGTAGAGCAGCTGATTAATGGACTGCCGGTGCCACCAACACCCTATTC 60
1245R-2.IR_full 61 TCTCGGGAACACCGCCTACCTGGCGCAGGAAACTTCGCAGGATCGACAGGCTCTATACCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1245R-2.in silico 61 TCTCGGGAACACCGCCTACCTGGCGCAGGAAACTTCGCAGGATCGACAGGCTCTATACCC 120
1245R-2.IR_full 121 TCAGCTGGTGAACAGCTACAAGTGGATGGACAAGGTGCACGACCACAGCTTCTTGGCCTT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1245R-2.in silico 121 TCAGCTGGTGAACAGCTACAAGTGGATGGACAAGGTGCACGACCACAGCTTCTTGGCCTT 180
1245R-2.IR_full 181 CAACAATCTCAACCAGAACACACTCAGGCGCTCCTACAATTACTGCTCGCAGAAAGCGCC 240
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
1245R-2.in silico 181 CAACAATCTCAACCAGAACACACTCAGGCGCTCCTACAATTACTGCTCGCAG-AAGCGCC 240
1245R-2.IR_full 241 AGCGACTGCCCTTCTCGTCCTTCAACAACGATCCAGACCACATAGATAAGCTCCTGAGTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1245R-2.in silico 241 AGCGACTGCCCTTCTCGTCCTTCAACAACGATCCAGACCACATAGATAAGCTCCTGAGTG 300
1245R-2.IR_full 301 AGATCAACACCCCTCCGCATACTTCGTACAGAATTTTTCGTCCCTTCGGCACAAACGCTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1245R-2.in silico 301 AGATCAACACCCCTCCGCATACTTCGTACAGAATTTTTCGTCCCTTCGGCACAAACGCTG 360
1245R-2.IR_full 361 TCACCATCGTAACAATAAGTAACGTGATGAAGGCGGCTATTGTCTTCAAAGGAGTGCTTA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1245R-2.in silico 361 TCACCATCGTAACAATAAGTAACGTGATGAAGGCGGCTATTGTCTTCAAAGGAGTGCTTA 420
1245R-2.IR_full 421 TAGAGTGGGTTACGGTTAAGGGATTCGATGAGCCGCTGGAGCATGACGACCTGTGGGCCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1245R-2.in silico 421 TAGAGTGGGTTACGGTTAAGGGATTCGATGAGCCGCTGGAGCATGACGACCTGTGGGCCG 480
1245R-2.IR_full 481 AGTCACGTTACGAGGTGTTCC 501
|||||||||||||||||||||
1245R-2.in silico 481 AGTCACGTTACGAGGTGTTCC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
4 |
NM_141312.2 |
CG1245-RA (MED27), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_169805.3 |
CG7125-RD, transcript variant D (PKD), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_142453.3 |
CG7125-RA, transcript variant A (PKD), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_169803.3 |
CG7125-RB, transcript variant B (PKD), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_169804.3 |
CG7125-RC, transcript variant C (PKD), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_079044.1 |
CG17876-RA (Amy-d), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_080421.3 |
CG18730-RA (Amy-p), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170444.1 |
CG7816-RF, transcript variant F (CG7816), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170445.1 |
CG7816-RG, transcript variant G (CG7816), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170440.1 |
CG7816-RA, transcript variant A (CG7816), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170441.1 |
CG7816-RC, transcript variant C (CG7816), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170443.1 |
CG7816-RE, transcript variant E (CG7816), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170442.1 |
CG7816-RD, transcript variant D (CG7816), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132980.1 |
CG8915-RA (CG8915), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169745.1 |
CG5175-RB, transcript variant B (kuk), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_142338.1 |
CG5175-RA, transcript variant A (kuk), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137386.2 |
CG18631-RA (CG18631), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_143427.2 |
CG1458-RA (CG1458), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_166568.1 |
CG3499-RB (CG3499), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_132914.2 |
CG9723-RA (CG9723), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_130703.2 |
CG16781-RA (CG16781), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001042838.1 |
CG40500-RC, transcript variant C (CG40500), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001042840.1 |
CG40500-RA, transcript variant A (CG40500), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001042839.1 |
CG40500-RB, transcript variant B (CG40500), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001042841.1 |
CG40500-RD, transcript variant D (CG40500), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_164857.1 |
CG3811-RA, transcript variant A (Oatp30B), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_136636.3 |
CG13739-RA (CG13739), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_057669.2 |
CG12399-RA (Mad), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_205946.1 |
CG3811-RC, transcript variant C (Oatp30B), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_135449.2 |
CG3811-RB, transcript variant B (Oatp30B), mRNA |
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