NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 12438R-3

Symbol CG12438 Full Name CG12438

CG No CG12438 Old CG No CG12438

Synonyms CG12438

Accession No (Link to NCBI) NM_135405.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGCGAGCAATGAGAACGAG

Primer Seq. 3'

aatctagaggtaccCCGATAGCCACATCCTGCCAA

Predicted Fragment Size

IR fragment full Seq

0001 ATGGCGAGCA ATGAGAACGA GAATGGCATA GCGCTGGTCA AGTTTGTAAC ATGGACCCTG 
0061 CTCATTTTGG GCATCTGTCT GCTGGCCGCC ATTCCGCAAT GGATGATCCT GTGTCTGTTT 
0121 GGAGATGGTG GCCTCATATA CACCATCGCC TGCTTTTTCG TGGGTTTCGT GCTGCTCGTC 
0181 TGCATCCATT TGATTGAGCC GCTAAAGTAC TGGAGGCCCT GGAACTATAT CGTCATCGCC 
0241 GTTTGCTACG AACTTATGAC ACTCGGGGCA GGAAGTTTTC TAATGAACTG GCGTCTGGTT 
0301 TGCTCCATTG TGACGATGGC CACGGCCCTG CNTTTTCCTC GGTATCACGC TGCTCATTTG 
0361 TGCCGTTCTA ATTAGCACTG GATTTTATAT GAACCCCTTC AAACTGGCCG TTGTGGGAGG 
0421 AATGCTATTC GTTCTGGCAT TCTGTATTGA ACTTATGAAT ATCCTGCTAG CGTGGAGGTA 
0481 TTGGCAGGAT GTGGCTATCG G

in silico PCR Fragment

0001 ATGGCGAGCA ATGAGAACGA GAATGGCATA GCGCTGGTCA AGTTTGTAAC ATGGACCCTG 
0061 CTCATTTTGG GCATCTGTTT GCTGGCCGCC ATTCCGCAAT GGATGATCCT GTGTCTGTTT 
0121 GGAGATGATG CCCTCATATA CACCATCGCC TGCTTTTTCG TGGGCTTCGT GCTGCTCGTC 
0181 TGCATCCATT TGATTGAGCC GCTGAAGTAC TGGAGGCCCT GGAACTATAT CGTCATCGCC 
0241 GTTTGCTACG AATTTATGAC ACTCGGGGCA GGAAGTTTTC TAATGAACTG GCGTCTGGTT 
0301 TGCTCCATTG TGACGATGGC CACGGCACTG C-TTTTCCTC GGTATCACGC TGCTCATTTG 
0361 TGCCGTTCTA ATTAGCACTG GATTTTATAT GAACCCCTTC AAACTGGCCG TTGTGGGAGG 
0421 AATGCTATTC GTTCTGGCAT TCTGTATTGA ACTTATGAAT ATCCTGCTAG CGTGGAGGTA 
0481 TTGGCAGGAT GTGGCTATCG G

Assemble Data


12438R-3.IR_full       1   ATGGCGAGCAATGAGAACGAGAATGGCATAGCGCTGGTCAAGTTTGTAACATGGACCCTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12438R-3.in silico     1   ATGGCGAGCAATGAGAACGAGAATGGCATAGCGCTGGTCAAGTTTGTAACATGGACCCTG 60


12438R-3.IR_full       61  CTCATTTTGGGCATCTGTCTGCTGGCCGCCATTCCGCAATGGATGATCCTGTGTCTGTTT 120
                           |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
12438R-3.in silico     61  CTCATTTTGGGCATCTGTTTGCTGGCCGCCATTCCGCAATGGATGATCCTGTGTCTGTTT 120


12438R-3.IR_full       121 GGAGATGGTGGCCTCATATACACCATCGCCTGCTTTTTCGTGGGTTTCGTGCTGCTCGTC 180
                           ||||||| || ||||||||||||||||||||||||||||||||| |||||||||||||||
12438R-3.in silico     121 GGAGATGATGCCCTCATATACACCATCGCCTGCTTTTTCGTGGGCTTCGTGCTGCTCGTC 180


12438R-3.IR_full       181 TGCATCCATTTGATTGAGCCGCTAAAGTACTGGAGGCCCTGGAACTATATCGTCATCGCC 240
                           ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
12438R-3.in silico     181 TGCATCCATTTGATTGAGCCGCTGAAGTACTGGAGGCCCTGGAACTATATCGTCATCGCC 240


12438R-3.IR_full       241 GTTTGCTACGAACTTATGACACTCGGGGCAGGAAGTTTTCTAATGAACTGGCGTCTGGTT 300
                           |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
12438R-3.in silico     241 GTTTGCTACGAATTTATGACACTCGGGGCAGGAAGTTTTCTAATGAACTGGCGTCTGGTT 300


12438R-3.IR_full       301 TGCTCCATTGTGACGATGGCCACGGCCCTGCNTTTTCCTCGGTATCACGCTGCTCATTTG 360
                           |||||||||||||||||||||||||| |||| ||||||||||||||||||||||||||||
12438R-3.in silico     301 TGCTCCATTGTGACGATGGCCACGGCACTGC-TTTTCCTCGGTATCACGCTGCTCATTTG 360


12438R-3.IR_full       361 TGCCGTTCTAATTAGCACTGGATTTTATATGAACCCCTTCAAACTGGCCGTTGTGGGAGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12438R-3.in silico     361 TGCCGTTCTAATTAGCACTGGATTTTATATGAACCCCTTCAAACTGGCCGTTGTGGGAGG 420


12438R-3.IR_full       421 AATGCTATTCGTTCTGGCATTCTGTATTGAACTTATGAATATCCTGCTAGCGTGGAGGTA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12438R-3.in silico     421 AATGCTATTCGTTCTGGCATTCTGTATTGAACTTATGAATATCCTGCTAGCGTGGAGGTA 480


12438R-3.IR_full       481 TTGGCAGGATGTGGCTATCGG 501
                           |||||||||||||||||||||
12438R-3.in silico     481 TTGGCAGGATGTGGCTATCGG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135405.1	CG12438-RA (CG12438), mRNA
0	0	1	4	2	NM_139889.1	CG7492-RA (CG7492), mRNA
0	0	1	3	6	NM_168418.1	CG14154-RA, transcript variant A (CG14154), mRNA
0	0	1	3	4	NM_140146.1	CG14154-RB, transcript variant B (CG14154), mRNA
0	0	1	3	2	NM_001043249.1	CG14864-RB, transcript variant B (CG14864), mRNA
0	0	1	3	2	NM_142182.1	CG14864-RA, transcript variant A (CG14864), mRNA
0	0	1	3	2	NM_134506.1	CG12531-RA (CG12531), mRNA
0	0	1	2	6	NM_079132.2	CG16910-RA (key), mRNA
0	0	1	2	4	NM_132998.1	CG8326-RA (CG8326), mRNA
0	0	1	2	4	NM_166019.1	CG18076-RH, transcript variant H (shot), mRNA
0	0	1	2	3	NM_168009.1	CG14959-RB, transcript variant B (CG14959), mRNA
0	0	1	2	3	NM_206264.1	CG14959-RC, transcript variant C (CG14959), mRNA
0	0	1	2	3	NM_139539.1	CG14959-RA, transcript variant A (CG14959), mRNA
0	0	1	2	2	NM_057977.3	CG9239-RA, transcript variant A (B4), mRNA
0	0	1	2	2	NM_165046.1	CG9239-RB, transcript variant B (B4), mRNA
0	0	0	5	7	NM_134675.4	CG11907-RB, transcript variant B (Ent1), mRNA
0	0	0	5	7	NM_164381.1	CG11907-RA, transcript variant A (Ent1), mRNA
0	0	0	5	4	NM_141832.2	CG5344-RA, transcript variant A (wkd), mRNA
0	0	0	5	4	NM_142248.2	CG31183-RA (CG31183), mRNA
0	0	0	5	3	NM_142521.1	CG6026-RA (CG6026), mRNA
0	0	0	5	3	NM_169402.2	CG5344-RB, transcript variant B (wkd), mRNA
0	0	0	4	9	NM_164887.1	CG31714-RA (CG31714), mRNA
0	0	0	4	4	NM_132224.1	CG10763-RA (Gbeta5), mRNA
0	0	0	4	4	NM_165971.1	CG4088-RB, transcript variant B (lat), mRNA
0	0	0	4	4	NM_057556.3	CG4088-RA, transcript variant A (lat), mRNA
0	0	0	4	3	NM_206712.1	CG33248-RA (CG33248), mRNA
0	0	0	4	3	NM_078495.1	CG15779-RA (Tre), mRNA
0	0	0	4	3	NM_143400.1	CG14517-RA (beta4GalNAcTB), mRNA
0	0	0	4	2	NM_131940.1	CG15912-RA (CG15912), mRNA
0	0	0	4	2	NM_137830.1	CG3701-RA (CG3701), mRNA

Back