National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12338R-2 
 Symbol CG12338  Full Name CG12338 
 CG No CG12338  Old CG No CG12338 
 Synonyms CG12338 
 Accession No (Link to NCBI) NM_136759.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGCGGTATCATTGGTCTTACG 
 Primer Seq. 3' 
aatctagaggtaccTGCGGCACTTCGAAAAAGCTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGCGGTATCA TTGGTCTTAC GACCGCCTTA GAACTCCAGA AGGAGTTTCC CACAGCAAGG 
0061 GTTTCCGTGA TTGCGGATCG GTTTAACGAG GACACCGTCA GCTATGTGGC GGCTGGAATT 
0121 TTCCGACCTG GAACCAGCTT TATGGGCCCC NCCCAGNAAA ATTACGCAAC AATGGATGAC 
0181 CGATGCCTTC AACTACTGGG ATGAGCTGCG TCGCTCCAAG GAGGCTCCTC TGGCGGGCGT 
0241 GTGCCAATTA TCCGGCTATA TTTACTCCCG CACCTCCCCC TCCATCGTAC GGAACCACTT 
0301 TATTGAGAAA CTATTGCCCG TCTACCGAAG AGCCACCGAG GAGGAACTGA GGCTATGCAA 
0361 CGGCGGCTGG AAGTACGGAT CCTTCTTCAC CACATGCCTA ACGGAAAGCC GCCTCTTCTT 
0421 GCCCTATGCC ACTAAAAAAT TCCTGGAGAA CGGAGGAGAG GTTGTCCGTC AGCATGTGAA 
0481 CAGCTTTTTC GANAGTgCCG CA 
 in silico PCR Fragment 
0001 AGCGGTATCA TTGGTCTTAC GACCGCCTTA GAACTCCAGA AGGAGTTTCC CACAGCAAGG 
0061 GTTTCCGTGA TTGCGGATCG GTTTAACGAG GACACCGTCA GCTATGTGGC GGCTGGAATT 
0121 TTCCGACCTG GAACCAGCTT TATGGGCCCC ACCCAG-AAA ATTACGCAAC AATGGATGAC 
0181 CGATGCCTTC AACTACTGGG ATGAGCTGCG TCGCTCCAAG GAGGCTCCTC TGGCGGGCGT 
0241 GTGCCAATTA TCCGGCTATA TTTACTCCCG CACCTCCCCC TCCATCGTAC GGAACCACTT 
0301 TATTGAGAAA CTATTGCCCG TCTACCGAAG AGCCACCGAG GAGGAACTGA GGCTATGCAA 
0361 CGGCGGCTGG AAGTACGGAT CCTTCTTCAC CACATGCCTA ACGGAAAGCC GCCTCTTCTT 
0421 GCCCTATGCC ACTAAAAAAT TCCTGGAGAA CGGAGGAGAG GTTGTCCGTC AGCATGTGAA 
0481 CAGCTTTTTC GA-AGTGCCG CA 
 Assemble Data 

12338R-2.IR_full       1   AGCGGTATCATTGGTCTTACGACCGCCTTAGAACTCCAGAAGGAGTTTCCCACAGCAAGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12338R-2.in silico     1   AGCGGTATCATTGGTCTTACGACCGCCTTAGAACTCCAGAAGGAGTTTCCCACAGCAAGG 60


12338R-2.IR_full       61  GTTTCCGTGATTGCGGATCGGTTTAACGAGGACACCGTCAGCTATGTGGCGGCTGGAATT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12338R-2.in silico     61  GTTTCCGTGATTGCGGATCGGTTTAACGAGGACACCGTCAGCTATGTGGCGGCTGGAATT 120


12338R-2.IR_full       121 TTCCGACCTGGAACCAGCTTTATGGGCCCCNCCCAGNAAAATTACGCAACAATGGATGAC 180
                           |||||||||||||||||||||||||||||| ||||| |||||||||||||||||||||||
12338R-2.in silico     121 TTCCGACCTGGAACCAGCTTTATGGGCCCCACCCAG-AAAATTACGCAACAATGGATGAC 180


12338R-2.IR_full       181 CGATGCCTTCAACTACTGGGATGAGCTGCGTCGCTCCAAGGAGGCTCCTCTGGCGGGCGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12338R-2.in silico     181 CGATGCCTTCAACTACTGGGATGAGCTGCGTCGCTCCAAGGAGGCTCCTCTGGCGGGCGT 240


12338R-2.IR_full       241 GTGCCAATTATCCGGCTATATTTACTCCCGCACCTCCCCCTCCATCGTACGGAACCACTT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12338R-2.in silico     241 GTGCCAATTATCCGGCTATATTTACTCCCGCACCTCCCCCTCCATCGTACGGAACCACTT 300


12338R-2.IR_full       301 TATTGAGAAACTATTGCCCGTCTACCGAAGAGCCACCGAGGAGGAACTGAGGCTATGCAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12338R-2.in silico     301 TATTGAGAAACTATTGCCCGTCTACCGAAGAGCCACCGAGGAGGAACTGAGGCTATGCAA 360


12338R-2.IR_full       361 CGGCGGCTGGAAGTACGGATCCTTCTTCACCACATGCCTAACGGAAAGCCGCCTCTTCTT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12338R-2.in silico     361 CGGCGGCTGGAAGTACGGATCCTTCTTCACCACATGCCTAACGGAAAGCCGCCTCTTCTT 420


12338R-2.IR_full       421 GCCCTATGCCACTAAAAAATTCCTGGAGAACGGAGGAGAGGTTGTCCGTCAGCATGTGAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12338R-2.in silico     421 GCCCTATGCCACTAAAAAATTCCTGGAGAACGGAGGAGAGGTTGTCCGTCAGCATGTGAA 480


12338R-2.IR_full       481 CAGCTTTTTCGANAGTGCCGCA 502
                           |||||||||||| |||||||||
12338R-2.in silico     481 CAGCTTTTTCGA-AGTGCCGCA 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_136759.2  CG12338-RA (CG12338), mRNA 
0   0  2  3  6  NM_140350.2  CG11009-RA (CG11009), mRNA 
0   0  2  2  7  NM_140346.1  CG10754-RA (CG10754), mRNA 
0   0  1  4  4  NM_141322.1  CG1236-RA (CG1236), mRNA 
0   0  1  4  4  NM_130635.2  CG3078-RA (CG3078), mRNA 
0   0  1  3  4  NM_135034.1  CG15624-RA, transcript variant A (hoe2), mRNA 
0   0  1  3  4  NM_001014466.1  CG15624-RB, transcript variant B (hoe2), mRNA 
0   0  1  3  2  NM_165019.2  CG5792-RB, transcript variant B (CG5792), mRNA 
0   0  1  3  2  NM_135746.2  CG5792-RA, transcript variant A (CG5792), mRNA 
0   0  1  2  4  NM_130679.2  CG3526-RB, transcript variant B (CG3526), mRNA 
0   0  1  2  4  NM_166954.1  CG3526-RA, transcript variant A (CG3526), mRNA 
0   0  0  7  4  NM_132546.2  CG1492-RA (CG1492), mRNA 
0   0  0  6  3  NM_132175.2  CG12155-RA (CG12155), mRNA 
0   0  0  5  4  NM_132948.3  CG9099-RA (CG9099), mRNA 
0   0  0  5  2  NM_078587.2  CG4353-RC, transcript variant C (hep), mRNA 
0   0  0  5  2  NM_206411.1  CG10512-RD, transcript variant D (CG10512), mRNA 
0   0  0  5  2  NM_141032.2  CG10512-RA, transcript variant A (CG10512), mRNA 
0   0  0  5  2  NM_168890.1  CG10512-RC, transcript variant C (CG10512), mRNA 
0   0  0  5  2  NM_168889.1  CG10512-RB, transcript variant B (CG10512), mRNA 
0   0  0  4  4  NM_143528.2  CG9682-RA (CG9682), mRNA 
0   0  0  4  2  NM_167346.1  CG4353-RA, transcript variant A (hep), mRNA 
0   0  0  4  2  NM_167060.1  CG32750-RA (CG32750), mRNA 
0   0  0  3  9  NM_164562.1  CG3964-RB, transcript variant B (CG3964), mRNA 
0   0  0  3  9  NM_134966.2  CG3964-RA, transcript variant A (CG3964), mRNA 
0   0  0  3  8  NM_132571.1  CG15731-RA (CG15731), mRNA 
0   0  0  3  6  NM_169996.1  CG5740-RA, transcript variant A (CG5740), mRNA 
0   0  0  3  6  NM_142753.2  CG5740-RB, transcript variant B (CG5740), mRNA 
0   0  0  3  4  NM_167275.1  CG32668-RA (CG32668), mRNA 
0   0  0  3  4  NM_139386.2  CG7974-RA (CG7974), mRNA 
0   0  0  3  4  NM_137711.3  CG30389-RA, transcript variant A (CG30389), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.