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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12236R-1 
 Symbol CG12236  Full Name CG12236 
 CG No CG12236  Old CG No CG12236 
 Synonyms CG12236 
 Accession No (Link to NCBI) NM_167054.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Strutt H, Searle E, Thomas-Macarthur V, Brookfield R, Strutt D.
A Cul-3-BTB ubiquitylation pathway regulates junctional levels and asymmetry of core planar polarity proteins.
Development (2013) 140(8) 1693-702 [ PubMed ID = 23487316 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGTACTCGCTGCGGTGGAACAA 
 Primer Seq. 3' 
aatctagaggtaccTGGCCGCGGGTATTTCGATGG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NTACTCGCTG CGGTGGAACA ACTACCTGCG CCACCTCACC TACTCCCTGG ACAACCACCG 
0061 GCTGAACGAT GACTTCGTGG ACGTCAGCCT GTGCGTGNGA TGGACGCAGG ATCAAGGCCC 
0121 ACAAGGTGGT ACTCTCCTCC TGCTCGTCCT ACTTTAAGGA GATATTCAAG GAGAACCCAC 
0181 ATCCGCATCC AGTCATTATA TTTAAGTTTA TCAAGTTCGA GGATCTCAAT TCGATTATCG 
0241 AGTTCATGTA CCAGGGCGAA GTCAATGTGC AGCAGGAGGC GCTGCAATCG TTCCTGCAAA 
0301 CTGCCGAGCT GCTGGCCGTC CAAGGACTCA CCGCCGAGGA GAAGGAGAAG CCGCAGATCC 
0361 CGGTGGCGCC GCTGATCAGC GAGCACAAGC TGATCAAGAC CATACCGAGC ACCATACGCG 
0421 CGGTCAACGA GCAGCAGCAG CAACAGCAGC AGCAGCAGCA GGTGTCCAAT GTGGCCCAGG 
0481 CGCAGACAGC TACGCAAACC ATCGAAATAN CcgCNGCCA 
 in silico PCR Fragment 
0001 GTACTCGCTG CGGTGGAACA ACTACCTGCG CCACCTCACC TACTCCCTGG ACAACCACCG 
0061 GCTGAACGAT GACTTCGTGG ACGTCAGCCT GTGCGTG-GA TGGACGCAGG ATCAAGGCCC 
0121 ACAAGGTGGT ACTCTCCTCC TGCTCGTCCT ACTTTAAGGA GATATTCAAG GAGAACCCAC 
0181 ATCCGCATCC AGTCATTATA TTTAAGTTTA TCAAGTTCGA GGATCTCAAT TCGATTATCG 
0241 AGTTCATGTA CCAGGGCGAA GTCAATGTGC AGCAGGAGGC GCTGCAATCG TTCCTGCAAA 
0301 CTGCCGAGCT GCTGGCCGTC CAAGGACTCA CCGCCGAGGA GAAGGAGAAG CCGCAGATCC 
0361 CGGTGGCGCC GCTGATCAGC GAGCACAAGC TGATCAAGAC CATACCGAGC ACCATACGCG 
0421 CGGTCAACG- ---------- -------AGC AGCAGCAGCA GGTGTCCAAT GTGGCCCAGG 
0481 CGCAGACAGC TACGCAAACC ATCGAAATAC CCGCGGCCA 
 Assemble Data 

12236R-1.IR_full        1   TACTCGCTGCGGTGGAACAACTACCTGCGCCACCTCACCTACTCCCTGGACAACCACCG 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12236R-1.in silico      1   TACTCGCTGCGGTGGAACAACTACCTGCGCCACCTCACCTACTCCCTGGACAACCACCG 59


12236R-1.IR_full       61  GCTGAACGATGACTTCGTGGACGTCAGCCTGTGCGTGNGATGGACGCAGGATCAAGGCCC 119
                           ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
12236R-1.in silico     61  GCTGAACGATGACTTCGTGGACGTCAGCCTGTGCGTG-GATGGACGCAGGATCAAGGCCC 119


12236R-1.IR_full       121 ACAAGGTGGTACTCTCCTCCTGCTCGTCCTACTTTAAGGAGATATTCAAGGAGAACCCAC 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12236R-1.in silico     121 ACAAGGTGGTACTCTCCTCCTGCTCGTCCTACTTTAAGGAGATATTCAAGGAGAACCCAC 179


12236R-1.IR_full       181 ATCCGCATCCAGTCATTATATTTAAGTTTATCAAGTTCGAGGATCTCAATTCGATTATCG 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12236R-1.in silico     181 ATCCGCATCCAGTCATTATATTTAAGTTTATCAAGTTCGAGGATCTCAATTCGATTATCG 239


12236R-1.IR_full       241 AGTTCATGTACCAGGGCGAAGTCAATGTGCAGCAGGAGGCGCTGCAATCGTTCCTGCAAA 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12236R-1.in silico     241 AGTTCATGTACCAGGGCGAAGTCAATGTGCAGCAGGAGGCGCTGCAATCGTTCCTGCAAA 299


12236R-1.IR_full       301 CTGCCGAGCTGCTGGCCGTCCAAGGACTCACCGCCGAGGAGAAGGAGAAGCCGCAGATCC 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12236R-1.in silico     301 CTGCCGAGCTGCTGGCCGTCCAAGGACTCACCGCCGAGGAGAAGGAGAAGCCGCAGATCC 359


12236R-1.IR_full       361 CGGTGGCGCCGCTGATCAGCGAGCACAAGCTGATCAAGACCATACCGAGCACCATACGCG 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12236R-1.in silico     361 CGGTGGCGCCGCTGATCAGCGAGCACAAGCTGATCAAGACCATACCGAGCACCATACGCG 419


12236R-1.IR_full       421 CGGTCAACGAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGGTGTCCAATGTGGCCCAGG 479
                           |||||||||                  |||||||||||||||||||||||||||||||||
12236R-1.in silico     421 CGGTCAACG------------------AGCAGCAGCAGCAGGTGTCCAATGTGGCCCAGG 479


12236R-1.IR_full       481 CGCAGACAGCTACGCAAACCATCGAAATANCCGCNGCCA 518
                           ||||||||||||||||||||||||||||| |||| ||||
12236R-1.in silico     481 CGCAGACAGCTACGCAAACCATCGAAATACCCGCGGCCA 518



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_167054.1  CG12236-RA, transcript variant A (CG12236), mRNA 
100   482  0  0  1  NM_132049.3  CG12236-RB, transcript variant B (CG12236), mRNA 
0.41   2  2  2  12  NM_130639.2  CG2918-RA (CG2918), mRNA 
0.2   1  4  7  11  NM_176138.3  CG12052-RL, transcript variant L (lola), mRNA 
0.2   1  4  7  11  NM_176131.3  CG12052-RP, transcript variant P (lola), mRNA 
0.2   1  4  7  11  NM_170621.3  CG12052-RE, transcript variant E (lola), mRNA 
0.2   1  4  7  11  NM_170620.2  CG12052-RD, transcript variant D (lola), mRNA 
0.2   1  4  7  10  NM_176134.2  CG12052-RT, transcript variant T (lola), mRNA 
0.2   1  4  7  10  NM_176135.3  CG12052-RU, transcript variant U (lola), mRNA 
0.2   1  4  7  10  NM_170622.4  CG12052-RF, transcript variant F (lola), mRNA 
0.2   1  4  7  10  NM_176136.3  CG12052-RK, transcript variant K (lola), mRNA 
0.2   1  4  7  10  NM_206085.2  CG12052-RV, transcript variant V (lola), mRNA 
0.2   1  4  7  10  NM_176137.3  CG12052-RS, transcript variant S (lola), mRNA 
0.2   1  4  7  10  NM_001032229.1  CG12052-RZ, transcript variant Z (lola), mRNA 
0.2   1  4  7  10  NM_170623.4  CG12052-RA, transcript variant A (lola), mRNA 
0.2   1  4  7  10  NM_176139.2  CG12052-RQ, transcript variant Q (lola), mRNA 
0.2   1  4  7  10  NM_176140.2  CG12052-RM, transcript variant M (lola), mRNA 
0.2   1  4  7  10  NM_176132.2  CG12052-RR, transcript variant R (lola), mRNA 
0.2   1  4  7  10  NM_080027.4  CG12052-RG, transcript variant G (lola), mRNA 
0.2   1  4  7  10  NM_206083.2  CG12052-RX, transcript variant X (lola), mRNA 
0.2   1  4  7  10  NM_206082.2  CG12052-RY, transcript variant Y (lola), mRNA 
0.2   1  4  7  10  NM_176128.3  CG12052-RN, transcript variant N (lola), mRNA 
0.2   1  4  7  10  NM_206084.2  CG12052-RW, transcript variant W (lola), mRNA 
0.2   1  4  7  10  NM_176129.3  CG12052-RO, transcript variant O (lola), mRNA 
0.2   1  4  7  10  NM_176130.3  CG12052-RJ, transcript variant J (lola), mRNA 
0.2   1  4  7  10  NM_170617.3  CG12052-RH, transcript variant H (lola), mRNA 
0.2   1  4  7  10  NM_170618.3  CG12052-RC, transcript variant C (lola), mRNA 
0.2   1  4  7  10  NM_170619.2  CG12052-RB, transcript variant B (lola), mRNA 
0.2   1  4  7  10  NM_176133.3  CG12052-RI, transcript variant I (lola), mRNA 
0   0  5  3  16  NM_132051.1  CG3726-RA (CG3726), mRNA 

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