National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12164R-2 
 Symbol CG12164  Full Name CG12164 
 CG No CG12164  Old CG No CG12164 
 Synonyms CG12164 
 Accession No (Link to NCBI) NM_136424.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGAGATCCCTAAGTCTGTTGC 
 Primer Seq. 3' 
aatctagaggtaccTTCGCCGGGAATGCCCATTTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGAGATCCCT AAGTCTGTTG CTACTCAGCG CCagCTGCGC GCTTAATCAT AAGCTTAGCC 
0061 TACGCCCATC CCCCCGAGGG AGTGTGGAAG AAGAAGCTCA CCTGGAAGGA GGATTGGGTC 
0121 CAGGTGTGGA AGACCGTTAA GAAGGAGGCG TGGGAAACCA AGTGGAAGAA GGTTTCGGTC 
0181 CCAATATGGA AGGAAGTCAA GGTTCCCGTC TGGAAGGAGG AGAAGGTTCC CGACTGGAAG 
0241 ATCGTAAAGA AGCCGAAAAT TGAGGAGAGA GAGGTGCCTG CCTGGAAGGA GGTTAAGGTG 
0301 GCCGAATGGA AGAAGATAAC GAAACCCATT TGGGTGCCCA CAAAGGTCGC CGTCTGGAAG 
0361 GAAATCCAGG TGCCCATTTG GAAGGAAGTG CAGGTCCCGT ACTGGAAGGA AATACAAGTG 
0421 CCCATTTGGA AGGAAGTACA GGTCGCAGAC TGGAAGCAAA TGTTTGAGCC CCAATGGGTG 
0481 AAAATGGGCA TTCCCGGCGA NGTA 
 in silico PCR Fragment 
0001 TGAGATCCCT AAGTCTGTTG CTACTCAGCG CCAGCTGCGC GC-TAATCAT AAGCTTAGCC 
0061 TACGCCCATC CCCCCGAGGG AGTGTGGAAG AAGAAGCTCA CCTGGAAGGA GGATTGGGTC 
0121 CAGGTGTGGA AGACCGTTAA GAAGGAGGCG TGGGAAACCA AGTGGAAGAA GGTTTCGGTC 
0181 CCAATATGGA AGGAAGTCAA GGTTCCCGTC TGGAAGGAGG AGAAGGTTCC CGACTGGAAG 
0241 ATCGTAAAGA AGCCGAAAAT TGAGGAGAGA GAGGTGCCTG CCTGGAAGGA GGTTAAGGTG 
0301 GCCGAATGGA AGAAGATAAC GAAACCCATT TGGGTGCCCA CAAAGGTCGC CGTCTGGAAG 
0361 GAAATCCAGG TGCCCATTTG GAAGGAAGTG CAGGTCCCGT ACTGGAAGGA AATACAAGTG 
0421 CCCATTTGGA AGGAAGTACA GGTCGCAGAC TGGAAGCAAA TGTTTGAGCC CCAATGGGTG 
0481 AAAATGGGCA TTCCCGGCGA ---A 
 Assemble Data 

12164R-2.IR_full       1   TGAGATCCCTAAGTCTGTTGCTACTCAGCGCCAGCTGCGCGCTTAATCATAAGCTTAGCC 60
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
12164R-2.in silico     1   TGAGATCCCTAAGTCTGTTGCTACTCAGCGCCAGCTGCGCGC-TAATCATAAGCTTAGCC 60


12164R-2.IR_full       61  TACGCCCATCCCCCCGAGGGAGTGTGGAAGAAGAAGCTCACCTGGAAGGAGGATTGGGTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12164R-2.in silico     61  TACGCCCATCCCCCCGAGGGAGTGTGGAAGAAGAAGCTCACCTGGAAGGAGGATTGGGTC 120


12164R-2.IR_full       121 CAGGTGTGGAAGACCGTTAAGAAGGAGGCGTGGGAAACCAAGTGGAAGAAGGTTTCGGTC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12164R-2.in silico     121 CAGGTGTGGAAGACCGTTAAGAAGGAGGCGTGGGAAACCAAGTGGAAGAAGGTTTCGGTC 180


12164R-2.IR_full       181 CCAATATGGAAGGAAGTCAAGGTTCCCGTCTGGAAGGAGGAGAAGGTTCCCGACTGGAAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12164R-2.in silico     181 CCAATATGGAAGGAAGTCAAGGTTCCCGTCTGGAAGGAGGAGAAGGTTCCCGACTGGAAG 240


12164R-2.IR_full       241 ATCGTAAAGAAGCCGAAAATTGAGGAGAGAGAGGTGCCTGCCTGGAAGGAGGTTAAGGTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12164R-2.in silico     241 ATCGTAAAGAAGCCGAAAATTGAGGAGAGAGAGGTGCCTGCCTGGAAGGAGGTTAAGGTG 300


12164R-2.IR_full       301 GCCGAATGGAAGAAGATAACGAAACCCATTTGGGTGCCCACAAAGGTCGCCGTCTGGAAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12164R-2.in silico     301 GCCGAATGGAAGAAGATAACGAAACCCATTTGGGTGCCCACAAAGGTCGCCGTCTGGAAG 360


12164R-2.IR_full       361 GAAATCCAGGTGCCCATTTGGAAGGAAGTGCAGGTCCCGTACTGGAAGGAAATACAAGTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12164R-2.in silico     361 GAAATCCAGGTGCCCATTTGGAAGGAAGTGCAGGTCCCGTACTGGAAGGAAATACAAGTG 420


12164R-2.IR_full       421 CCCATTTGGAAGGAAGTACAGGTCGCAGACTGGAAGCAAATGTTTGAGCCCCAATGGGTG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12164R-2.in silico     421 CCCATTTGGAAGGAAGTACAGGTCGCAGACTGGAAGCAAATGTTTGAGCCCCAATGGGTG 480


12164R-2.IR_full       481 AAAATGGGCATTCCCGGCGANGTA 504
                           ||||||||||||||||||||   |
12164R-2.in silico     481 AAAATGGGCATTCCCGGCGA---A 504



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100.82  486  23  73  147  NM_136424.2  CG12164-RA (CG12164), mRNA 
0.2   1  2  3  4  NM_166637.1  CG3725-RF, transcript variant F (Ca-P60A), mRNA 
0.2   1  2  3  4  NM_166634.1  CG3725-RC, transcript variant C (Ca-P60A), mRNA 
0.2   1  2  3  4  NM_166635.1  CG3725-RD, transcript variant D (Ca-P60A), mRNA 
0.2   1  2  3  4  NM_166633.1  CG3725-RB, transcript variant B (Ca-P60A), mRNA 
0.2   1  2  3  4  NM_166636.1  CG3725-RE, transcript variant E (Ca-P60A), mRNA 
0.2   1  2  3  4  NM_166638.1  CG3725-RG, transcript variant G (Ca-P60A), mRNA 
0.2   1  2  3  4  NM_166639.1  CG3725-RH, transcript variant H (Ca-P60A), mRNA 
0.2   1  2  3  4  NM_057484.2  CG3725-RA, transcript variant A (Ca-P60A), mRNA 
0   0  2  5  2  NM_139497.1  CG9970-RA (CG9970), mRNA 
0   0  2  3  10  NM_169442.1  CG12213-RA, transcript variant A (CG12213), mRNA 
0   0  2  3  10  NM_141876.1  CG12213-RB, transcript variant B (CG12213), mRNA 
0   0  2  2  5  NM_134953.2  CG16704-RA (CG16704), mRNA 
0   0  1  15  715  NM_001015110.1  CG40323-PB.3 (CG40323), mRNA 
0   0  1  5  4  NM_057806.3  CG13279-RA (Cyt-b5-r), mRNA 
0   0  1  4  26  NM_001015309.1  CG40373-PA.3 (CG40373), mRNA 
0   0  1  4  9  NM_170306.1  CG31077-RA (CG31077), mRNA 
0   0  1  4  6  NM_166073.1  CG10249-RC, transcript variant C (CG10249), mRNA 
0   0  1  4  6  NM_166074.1  CG10249-RB, transcript variant B (CG10249), mRNA 
0   0  1  4  6  NM_137161.1  CG10249-RA, transcript variant A (CG10249), mRNA 
0   0  1  4  5  NM_130545.2  CG11411-RA (fs(1)N), mRNA 
0   0  0  23  50  NM_137315.1  CG15615-RA (CG15615), mRNA 
0   0  0  6  9  NM_165224.1  CG7100-RH, transcript variant H (CadN), mRNA 
0   0  0  6  9  NM_165225.1  CG7100-RB, transcript variant B (CadN), mRNA 
0   0  0  6  9  NM_165226.1  CG7100-RF, transcript variant F (CadN), mRNA 
0   0  0  6  9  NM_165227.1  CG7100-RD, transcript variant D (CadN), mRNA 
0   0  0  6  9  NM_165228.1  CG7100-RC, transcript variant C (CadN), mRNA 
0   0  0  6  9  NM_165229.1  CG7100-RA, transcript variant A (CadN), mRNA 
0   0  0  6  9  NM_165230.1  CG7100-RG, transcript variant G (CadN), mRNA 
0   0  0  6  9  NM_165231.1  CG7100-RE, transcript variant E (CadN), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.