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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12155R-2 
 Symbol CG12155  Full Name CG12155 
 CG No CG12155  Old CG No CG12155 
 Synonyms CG12155 
 Accession No (Link to NCBI) NM_132175.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees male lethal, abnormal wing 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGGACGACATCCTTACGCTGA 
 Primer Seq. 3' 
aatctagaggtaccGGTGCCGTGATGTGAGGAGAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGGACGACAT CCTTACGCTG ATCAACCTGG TGCGCCAGAA TCCGGTTCTC TACAACTACA 
0061 AACTGCAGCC GAACCAGCGA CGCCGCTCCG ATGTCCTCAA CGGATGGCAG GAGGTGGCCC 
0121 AGCAGATAGG AAACAAGTAC ACGGTGCAGG AGGTGCGTCG TAAGTGGAAG AACCTGCGGG 
0181 ACACTTTCCA CCAGTACCGT CTGCGCACGC CCAAGTACAT CGAAGGGCGG CTATCCAANG 
0241 TGGCGCTACG CCAAGGAGCT GGACTTCCTG TCGAAGGTGT ACCAGCCGAA ACTGAAATCG 
0301 CACCGGAACA CACAGATCAC CTATGAGACG AGTGGGGTTN GCCGGCGCCG GTGGAGGCAT 
0361 TGGAGGCGCG AATAGTACCA CATTGCCCAT TGGAGCTATG CTGCATTTGA AACAGCATGT 
0421 GGACGATGAC GACGACGAGG TGATGGACGA TGACGGGCAA TCGGATCACG ATACCGCCAC 
0481 GCTCTCCTCA CATCACGGCA CC 
 in silico PCR Fragment 
0001 CGGACGACAT CCTTACGCTG ATCAACCTGG TGCGCCAGAA TCCGGTTCTC TACAACTACA 
0061 AACTGCAGCC GAACCAGCGA CGCCGCTCCG ATGTCCTCAA CGGATGGCAG GAGGTGGCCC 
0121 AGCAGATAGG AAACAAGTAC ACGGTGCAGG AGGTGCGTCG TAAGTGGAAG AACCTGCGGG 
0181 ACACTTTCCA CCAGTACCGT CTGCGCACGC CCAAGTACAT CGAAGGGCGG CTATCCAA-G 
0241 TGGCGCTACG CCAAGGAGCT GGACTTCCTG TCGAAGGTGT ACCAGCCGAA ACTGAAATCG 
0301 CACCGGAACA CACAGATCAC CTATGAGACG AGTGGGGTT- GCCGGCGCCG GTGGAGGCAT 
0361 TGGAGGCGCG AATAGTACCA CATTGCCCAT TGGAGCTATG CTGCATTTGA AACAGCATGT 
0421 GGACGATGAC GACGACGAGG TGATGGACGA TGACGGGCAA TCGGATCACG ATACCGCCAC 
0481 GCTCTCCTCA CATCACGGCA CC 
 Assemble Data 

12155R-2.IR_full       1   CGGACGACATCCTTACGCTGATCAACCTGGTGCGCCAGAATCCGGTTCTCTACAACTACA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12155R-2.in silico     1   CGGACGACATCCTTACGCTGATCAACCTGGTGCGCCAGAATCCGGTTCTCTACAACTACA 60


12155R-2.IR_full       61  AACTGCAGCCGAACCAGCGACGCCGCTCCGATGTCCTCAACGGATGGCAGGAGGTGGCCC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12155R-2.in silico     61  AACTGCAGCCGAACCAGCGACGCCGCTCCGATGTCCTCAACGGATGGCAGGAGGTGGCCC 120


12155R-2.IR_full       121 AGCAGATAGGAAACAAGTACACGGTGCAGGAGGTGCGTCGTAAGTGGAAGAACCTGCGGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12155R-2.in silico     121 AGCAGATAGGAAACAAGTACACGGTGCAGGAGGTGCGTCGTAAGTGGAAGAACCTGCGGG 180


12155R-2.IR_full       181 ACACTTTCCACCAGTACCGTCTGCGCACGCCCAAGTACATCGAAGGGCGGCTATCCAANG 240
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
12155R-2.in silico     181 ACACTTTCCACCAGTACCGTCTGCGCACGCCCAAGTACATCGAAGGGCGGCTATCCAA-G 240


12155R-2.IR_full       241 TGGCGCTACGCCAAGGAGCTGGACTTCCTGTCGAAGGTGTACCAGCCGAAACTGAAATCG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12155R-2.in silico     241 TGGCGCTACGCCAAGGAGCTGGACTTCCTGTCGAAGGTGTACCAGCCGAAACTGAAATCG 300


12155R-2.IR_full       301 CACCGGAACACACAGATCACCTATGAGACGAGTGGGGTTNGCCGGCGCCGGTGGAGGCAT 360
                           ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
12155R-2.in silico     301 CACCGGAACACACAGATCACCTATGAGACGAGTGGGGTT-GCCGGCGCCGGTGGAGGCAT 360


12155R-2.IR_full       361 TGGAGGCGCGAATAGTACCACATTGCCCATTGGAGCTATGCTGCATTTGAAACAGCATGT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12155R-2.in silico     361 TGGAGGCGCGAATAGTACCACATTGCCCATTGGAGCTATGCTGCATTTGAAACAGCATGT 420


12155R-2.IR_full       421 GGACGATGACGACGACGAGGTGATGGACGATGACGGGCAATCGGATCACGATACCGCCAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12155R-2.in silico     421 GGACGATGACGACGACGAGGTGATGGACGATGACGGGCAATCGGATCACGATACCGCCAC 480


12155R-2.IR_full       481 GCTCTCCTCACATCACGGCACC 502
                           ||||||||||||||||||||||
12155R-2.in silico     481 GCTCTCCTCACATCACGGCACC 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_132175.2  CG12155-RA (CG12155), mRNA 
0.41   2  2  3  2  NM_079543.2  CG1257-RA (alpha-Est3), mRNA 
0.2   1  2  6  30  NM_001032052.1  CG33715-RE, transcript variant E (Msp-300), mRNA 
0.2   1  2  5  2  NM_164480.1  CG4272-RB, transcript variant B (CG4272), mRNA 
0.2   1  2  5  2  NM_134829.2  CG4272-RA, transcript variant A (CG4272), mRNA 
0.2   1  2  3  10  NM_143409.1  CG11873-RA (CG11873), mRNA 
0   0  5  6  10  NM_078524.2  CG2262-RA (Smox), mRNA 
0   0  4  10  12  NM_057597.2  CG10719-RA, transcript variant A (brat), mRNA 
0   0  4  10  12  NM_134302.1  CG10719-RB, transcript variant B (brat), mRNA 
0   0  4  10  12  NM_206004.1  CG10719-RC, transcript variant C (brat), mRNA 
0   0  4  4  4  NM_165243.1  CG15154-RA, transcript variant A (Socs36E), mRNA 
0   0  4  4  4  NM_078869.4  CG15154-RB, transcript variant B (Socs36E), mRNA 
0   0  4  3  10  NM_001043218.1  CG33097-RB, transcript variant B (CG33097), mRNA 
0   0  4  3  10  NM_176408.1  CG33097-RA, transcript variant A (CG33097), mRNA 
0   0  4  2  4  NM_141416.3  CG15179-RA (sunz), mRNA 
0   0  4  2  2  NM_135145.1  CG9144-RA (CG9144), mRNA 
0   0  3  2  4  NM_137815.2  CG3380-RA (Oatp58Dc), mRNA 
0   0  2  12  26  NM_167387.1  CG32611-RB (CG32611), mRNA 
0   0  2  11  34  NM_132831.1  CG8184-RB (CG8184), mRNA 
0   0  2  10  37  NM_079967.4  CG6611-RA, transcript variant A (ect), mRNA 
0   0  2  10  37  NM_168400.1  CG6611-RB, transcript variant B (ect), mRNA 
0   0  2  10  37  NM_168401.1  CG6611-RC, transcript variant C (ect), mRNA 
0   0  2  5  8  NM_132529.2  CG2025-RA (CG2025), mRNA 
0   0  2  4  9  NM_170345.1  CG31058-RA (CG31058), mRNA 
0   0  2  4  6  NM_138225.2  CG13907-RA (CG13907), mRNA 
0   0  2  3  15  NM_078604.3  CG32592-RA (hiw), mRNA 
0   0  2  2  8  NM_169941.1  CG5685-RC, transcript variant C (Calx), mRNA 
0   0  2  2  8  NM_169940.1  CG5685-RB, transcript variant B (Calx), mRNA 
0   0  2  2  8  NM_079699.2  CG5685-RA, transcript variant A (Calx), mRNA 
0   0  2  2  3  NM_166824.2  CG11155-RB, transcript variant B (CG11155), mRNA 

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