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NIG-RNAi Strains Detail |
Request : Click the "Order" button to request for this stock |
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
Stock ID |
12114R-1 |
Symbol |
spn-F |
Full Name |
spn-F |
CG No |
CG12114 |
Old CG No |
CG12114 |
Synonyms |
spn-F, CG12114, 1130/03, l(3)S113003b, Spn-F |
Accession No (Link to NCBI) |
NM_143601.2 |
Inserted Chr. |
lll |
Insertional Mutation |
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Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
Map Viewer |
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Reference
[Please submit your publication]
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Cho Y, Lai CM, Lin KY, Hsu HJ. A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development. G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Fairchild MJ, Islam F, Tanentzapf G. Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis. PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T. RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc. Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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Comment |
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Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
Primer Seq. 5' |
aaggcctacatggccggaccgAGCTAAGGGAGGCCTCCAGCA |
Primer Seq. 3' |
aatctagaggtaccCTTCATGGCCTCTCGCCTCAG |
Predicted Fragment Size |
500 |
IR fragment full Seq |
0001 AGCTAAGGGA GGCCTCCAGC AGGTCGGAAG GTGCTCCAAG GGCAAACGAA ATCGGGGTCA
0061 CTGGGGACGT CCTCTCTCTG AAAGCACAGG TCTCTGAGCT GCAGCGCCAA AAAGAGCAGC
0121 TGGAGGAGCA CATTGGCATG GTATCCAACG AGAACAGACG GCTCTGGTCC CGCCTATCGC
0181 AGATCTCCAA GGATCAGCAG CTGAACGCAC TGCCCAGCTC AACAGACTCG CGAGCCCAGC
0241 AAAACCAGAA CCTGGTGCGC TCCAAGACCT TTACGCAGCA CTCCCCCAAT CCTCACCTTC
0301 GCCAAAAGAT GCTCTCCGAC GGCATTAAGG ATCTCAGTCT GGAGGAAATA GCCCTGGATG
0361 ACTTCGGTGC CAGCAGTGAG GAACTAGGTT ACCCCTACAA CCTGCAAAAG GTGGAGGAAA
0421 CCACTAGTGA ACCAGATGCC AATGTGGATG CCAAAAGATG TTTGGATGGA CTGCAGGAGC
0481 TGAGGCGAGA GGCCATGAAG |
in silico PCR Fragment |
0001 AGCTAAGGGA GGCCTCCAGC AGGTCGGAAG GTGCTCCAAG GGCAAACGAA ATCGGGGTCA
0061 CTGGGGACGT CCTCTCTCTG AAAGCACAGG TCTCTGAGCT GCAGCGCCAA AAGGAGCAGC
0121 TGGAGGAGCA CATTGGCATG GTATCCAACG AGAACAGACG GCTCTGGTCC CGCCTATCGC
0181 AGATCTCCAA GGATCAGCAG CTGAACGCAC TGCCCAGCTC AACAGACTCG CGAGCCCAGC
0241 AAAACCAGAA CCTGGTGCGC TCCAAGACCT TTACGCAGCA CTCCCCCAAT CCTCACCTTC
0301 GCCAAAAGAT GCTCTCCGAC GGCATTAAGG ATCTCAGTCT GGAGGAAATA GCCCTGGATG
0361 ACTTCGGTGC CAGCAGTGAG GAACTAGGTT ACCCCTACAA CCTGCAAAAG GTGGAGGAAA
0421 CCACTAGTGA ACCAGATGCC AATGTGGATG CCAAAAGATG TTTGGATGGA CTGCAGGAGC
0481 TGAGGCGAGA GGCCATGAAG |
Assemble Data |
12114R-1.IR_full 1 AGCTAAGGGAGGCCTCCAGCAGGTCGGAAGGTGCTCCAAGGGCAAACGAAATCGGGGTCA 60
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12114R-1.in silico 1 AGCTAAGGGAGGCCTCCAGCAGGTCGGAAGGTGCTCCAAGGGCAAACGAAATCGGGGTCA 60
12114R-1.IR_full 61 CTGGGGACGTCCTCTCTCTGAAAGCACAGGTCTCTGAGCTGCAGCGCCAAAAAGAGCAGC 120
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12114R-1.in silico 61 CTGGGGACGTCCTCTCTCTGAAAGCACAGGTCTCTGAGCTGCAGCGCCAAAAGGAGCAGC 120
12114R-1.IR_full 121 TGGAGGAGCACATTGGCATGGTATCCAACGAGAACAGACGGCTCTGGTCCCGCCTATCGC 180
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12114R-1.in silico 121 TGGAGGAGCACATTGGCATGGTATCCAACGAGAACAGACGGCTCTGGTCCCGCCTATCGC 180
12114R-1.IR_full 181 AGATCTCCAAGGATCAGCAGCTGAACGCACTGCCCAGCTCAACAGACTCGCGAGCCCAGC 240
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12114R-1.in silico 181 AGATCTCCAAGGATCAGCAGCTGAACGCACTGCCCAGCTCAACAGACTCGCGAGCCCAGC 240
12114R-1.IR_full 241 AAAACCAGAACCTGGTGCGCTCCAAGACCTTTACGCAGCACTCCCCCAATCCTCACCTTC 300
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12114R-1.in silico 241 AAAACCAGAACCTGGTGCGCTCCAAGACCTTTACGCAGCACTCCCCCAATCCTCACCTTC 300
12114R-1.IR_full 301 GCCAAAAGATGCTCTCCGACGGCATTAAGGATCTCAGTCTGGAGGAAATAGCCCTGGATG 360
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12114R-1.in silico 301 GCCAAAAGATGCTCTCCGACGGCATTAAGGATCTCAGTCTGGAGGAAATAGCCCTGGATG 360
12114R-1.IR_full 361 ACTTCGGTGCCAGCAGTGAGGAACTAGGTTACCCCTACAACCTGCAAAAGGTGGAGGAAA 420
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12114R-1.in silico 361 ACTTCGGTGCCAGCAGTGAGGAACTAGGTTACCCCTACAACCTGCAAAAGGTGGAGGAAA 420
12114R-1.IR_full 421 CCACTAGTGAACCAGATGCCAATGTGGATGCCAAAAGATGTTTGGATGGACTGCAGGAGC 480
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12114R-1.in silico 421 CCACTAGTGAACCAGATGCCAATGTGGATGCCAAAAGATGTTTGGATGGACTGCAGGAGC 480
12114R-1.IR_full 481 TGAGGCGAGAGGCCATGAAG 500
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12114R-1.in silico 481 TGAGGCGAGAGGCCATGAAG 500
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Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
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cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
100 |
482 |
0 |
0 |
0 |
NM_143601.2 |
CG12114-RA (CG12114), mRNA |
0.41 |
2 |
6 |
6 |
8 |
NM_135369.1 |
CG14273-RA (CG14273), mRNA |
0.41 |
2 |
4 |
4 |
2 |
NM_167087.1 |
CG3203-RA, transcript variant A (RpL17), mRNA |
0.41 |
2 |
4 |
4 |
2 |
NM_132118.2 |
CG3203-RD, transcript variant D (RpL17), mRNA |
0.41 |
2 |
4 |
4 |
2 |
NM_167089.1 |
CG3203-RC, transcript variant C (RpL17), mRNA |
0.41 |
2 |
4 |
4 |
2 |
NM_167088.1 |
CG3203-RB, transcript variant B (RpL17), mRNA |
0.2 |
1 |
2 |
4 |
5 |
NM_133138.1 |
CG7884-RA (CG7884), mRNA |
0.2 |
1 |
2 |
3 |
7 |
NM_134833.4 |
CG9866-RA, transcript variant A (CG9866), mRNA |
0.2 |
1 |
2 |
3 |
7 |
NM_164481.1 |
CG9866-RB, transcript variant B (CG9866), mRNA |
0.2 |
1 |
2 |
3 |
3 |
NM_001042822.1 |
CG34120-RC, transcript variant C (CG34120), mRNA |
0 |
0 |
3 |
4 |
9 |
NM_134680.1 |
CG13691-RA (BBS8), mRNA |
0 |
0 |
3 |
2 |
7 |
NM_134804.3 |
CG7261-RA (CG7261), mRNA |
0 |
0 |
3 |
2 |
4 |
NM_142172.2 |
CG6654-RA (CG6654), mRNA |
0 |
0 |
2 |
5 |
4 |
NM_166535.1 |
CG4444-RA (px), mRNA |
0 |
0 |
2 |
4 |
3 |
NM_140908.1 |
CG7570-RA (hale), mRNA |
0 |
0 |
2 |
4 |
2 |
NM_140350.2 |
CG11009-RA (CG11009), mRNA |
0 |
0 |
2 |
3 |
5 |
NM_001043272.1 |
CG17299-RK, transcript variant K (SNF4Agamma), mRNA |
0 |
0 |
2 |
3 |
5 |
NM_001043273.1 |
CG17299-RJ, transcript variant J (SNF4Agamma), mRNA |
0 |
0 |
2 |
3 |
5 |
NM_169949.1 |
CG17299-RE, transcript variant E (SNF4Agamma), mRNA |
0 |
0 |
2 |
3 |
5 |
NM_169948.1 |
CG17299-RC, transcript variant C (SNF4Agamma), mRNA |
0 |
0 |
2 |
3 |
4 |
NM_078514.2 |
CG9653-RA (brk), mRNA |
0 |
0 |
2 |
3 |
3 |
NM_169946.1 |
CG17299-RA, transcript variant A (SNF4Agamma), mRNA |
0 |
0 |
2 |
3 |
3 |
NM_080509.2 |
CG17299-RB, transcript variant B (SNF4Agamma), mRNA |
0 |
0 |
2 |
3 |
3 |
NM_169947.1 |
CG17299-RF, transcript variant F (SNF4Agamma), mRNA |
0 |
0 |
2 |
3 |
3 |
NM_001043271.1 |
CG17299-RL, transcript variant L (SNF4Agamma), mRNA |
0 |
0 |
2 |
3 |
3 |
NM_169950.1 |
CG17299-RG, transcript variant G (SNF4Agamma), mRNA |
0 |
0 |
2 |
3 |
2 |
NM_001043274.1 |
CG17299-RI, transcript variant I (SNF4Agamma), mRNA |
0 |
0 |
2 |
2 |
4 |
NM_139783.1 |
CG10226-RA (CG10226), mRNA |
0 |
0 |
2 |
2 |
4 |
NM_169458.1 |
CG11502-RA, transcript variant A (svp), mRNA |
0 |
0 |
2 |
2 |
3 |
NM_142482.2 |
CG7700-RA (Gos28), mRNA |
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Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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