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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12085R-4 |
| Symbol |
pUf68 |
Full Name |
poly U binding factor 68kD |
| CG No |
CG12085 |
Old CG No |
CG12085 |
| Synonyms |
hfp, PUF60, DPUF68, HFP, pUbsf, dFIR, Puf60, CG12085, Hfp, Fir, anon-WO0118547.408, pUf68 |
| Accession No (Link to NCBI) |
NM_080384.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Mitchell NC, Johanson TM, Cranna NJ, Er AL, Richardson HE, Hannan RD, Quinn LM.
Hfp inhibits Drosophila myc transcription and cell growth in a TFIIH/Hay-dependent manner.
Development (2010) 137(17) 2875-84
[
PubMed ID = 20667914
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAAGTACGCCATGGAGCAGAGC |
| Primer Seq. 3' |
aatctagaggtaccCAGTAGAGGATCGGGCCAAAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AAGTACGCCA TGGAGCAGAN CATCAAGATG GTGTTGATGA AGCAGACACT GGCCCACCAA
0061 CAGCAGCAAC TGGCCACTCA GCGCACGCAG GTTCAGCGAC AACAGGCACT GGCTCTCATG
0121 TGCAGAGTCT ACGTGGGCAG CATTTCGTTT GAGCTCAAGG AGGACACAAT TCGTGTGGCC
0181 TTCACTCCCT TCGGCCCCAT CAAATCAATC AACATGTCCT GGGATCCCAT CACGCAAAAG
0241 CACAAGGGAT TCGCGTTCGT GGAGTATGAG ATACCGGAGG GCGCACAgCT GGCGCTGGAA
0301 CAGATGAACG GAGCCCTGAT GGGGGGCAGG AACATCAAGG TAGGCCGACC CAGTAACATG
0361 CCACAGGCGC AGCAGGTGAT CGACGAGGTG CAGGAGGAGG CCAAGAGCTT CAACCGCATC
0421 TACGTGGCCT CCATCCATCC CGACCTTTCC GAGGAGGACA TCAAGAGCGT CTTCGAggcN
0481 TTTGGCCCGA TCCTCTACTG |
| in silico PCR Fragment |
0001 AAGTACGCCA TGGAGCAGAG CATCAAGATG GTGTTGATGA AGCAGACACT GGCCCACCAA
0061 CAGCAGCAAT TGGCCACTCA GCGCACGCAG GTTCAGCGAC AACAGGCACT GGCTCTCATG
0121 TGCAGAGTCT ACGTGGGCAG CATTTCGTTT GAGCTCAAGG AGGACACAAT TCGTGTGGCC
0181 TTCACTCCCT TCGGCCCCAT CAAATCAATC AACATGTCCT GGGATCCCAT CACGCAAAAG
0241 CACAAGGGAT TCGCGTTCGT GGAGTATGAG ATACCGGAGG GCGCACAGCT GGCGCTGGAA
0301 CAGATGAACG GAGCCCTGAT GGGGGGCAGG AACATCAAGG TAGGCCGACC CAGTAACATG
0361 CCACAGGCGC AGCAGGTGAT CGACGAGGTG CAGGAGGAGG CCAAGAGCTT CAACCGCATC
0421 TACGTGGCCT CCATCCATCC CGACCTTTCC GAGGAGGACA TCAAGAGCGT CTTCGAGGCC
0481 TTTGGCCCGA TCCTCTACTG |
| Assemble Data |
12085R-4.IR_full 1 AAGTACGCCATGGAGCAGANCATCAAGATGGTGTTGATGAAGCAGACACTGGCCCACCAA 60
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
12085R-4.in silico 1 AAGTACGCCATGGAGCAGAGCATCAAGATGGTGTTGATGAAGCAGACACTGGCCCACCAA 60
12085R-4.IR_full 61 CAGCAGCAACTGGCCACTCAGCGCACGCAGGTTCAGCGACAACAGGCACTGGCTCTCATG 120
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
12085R-4.in silico 61 CAGCAGCAATTGGCCACTCAGCGCACGCAGGTTCAGCGACAACAGGCACTGGCTCTCATG 120
12085R-4.IR_full 121 TGCAGAGTCTACGTGGGCAGCATTTCGTTTGAGCTCAAGGAGGACACAATTCGTGTGGCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12085R-4.in silico 121 TGCAGAGTCTACGTGGGCAGCATTTCGTTTGAGCTCAAGGAGGACACAATTCGTGTGGCC 180
12085R-4.IR_full 181 TTCACTCCCTTCGGCCCCATCAAATCAATCAACATGTCCTGGGATCCCATCACGCAAAAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12085R-4.in silico 181 TTCACTCCCTTCGGCCCCATCAAATCAATCAACATGTCCTGGGATCCCATCACGCAAAAG 240
12085R-4.IR_full 241 CACAAGGGATTCGCGTTCGTGGAGTATGAGATACCGGAGGGCGCACAGCTGGCGCTGGAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12085R-4.in silico 241 CACAAGGGATTCGCGTTCGTGGAGTATGAGATACCGGAGGGCGCACAGCTGGCGCTGGAA 300
12085R-4.IR_full 301 CAGATGAACGGAGCCCTGATGGGGGGCAGGAACATCAAGGTAGGCCGACCCAGTAACATG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12085R-4.in silico 301 CAGATGAACGGAGCCCTGATGGGGGGCAGGAACATCAAGGTAGGCCGACCCAGTAACATG 360
12085R-4.IR_full 361 CCACAGGCGCAGCAGGTGATCGACGAGGTGCAGGAGGAGGCCAAGAGCTTCAACCGCATC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12085R-4.in silico 361 CCACAGGCGCAGCAGGTGATCGACGAGGTGCAGGAGGAGGCCAAGAGCTTCAACCGCATC 420
12085R-4.IR_full 421 TACGTGGCCTCCATCCATCCCGACCTTTCCGAGGAGGACATCAAGAGCGTCTTCGAGGCN 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12085R-4.in silico 421 TACGTGGCCTCCATCCATCCCGACCTTTCCGAGGAGGACATCAAGAGCGTCTTCGAGGCC 480
12085R-4.IR_full 481 TTTGGCCCGATCCTCTACTG 500
||||||||||||||||||||
12085R-4.in silico 481 TTTGGCCCGATCCTCTACTG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_167881.1 |
CG12085-RC, transcript variant C (pUf68), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_167882.1 |
CG12085-RD, transcript variant D (pUf68), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_080384.2 |
CG12085-RA, transcript variant A (pUf68), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_167880.1 |
CG12085-RB, transcript variant B (pUf68), mRNA |
| 0.62 |
3 |
2 |
3 |
4 |
NM_141216.2 |
CG14650-RA (CG14650), mRNA |
| 0.2 |
1 |
2 |
3 |
2 |
NM_142387.1 |
CG7643-RA (ald), mRNA |
| 0 |
0 |
3 |
6 |
3 |
NM_143315.1 |
CG5612-RA (CG5612), mRNA |
| 0 |
0 |
2 |
8 |
17 |
NM_168413.1 |
CG32062-RB, transcript variant B (CG32062), mRNA |
| 0 |
0 |
2 |
8 |
17 |
NM_168412.1 |
CG32062-RD, transcript variant D (CG32062), mRNA |
| 0 |
0 |
2 |
3 |
25 |
NM_078797.2 |
CG13109-RA (tai), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_078601.2 |
CG9533-RA (rut), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_169060.1 |
CG9533-RA (rut), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_169061.1 |
CG9533-RA (rut), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_168614.2 |
CG6988-RD, transcript variant D (Pdi), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_079355.2 |
CG6988-RA, transcript variant A (Pdi), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_165046.1 |
CG9239-RB, transcript variant B (B4), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_057977.3 |
CG9239-RA, transcript variant A (B4), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_140783.2 |
CG7285-RA (star1), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_132666.1 |
CG15754-RA (CG15754), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_001032244.1 |
CG32904-RA, transcript variant A (seq), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_136983.2 |
CG17019-RA (CG17019), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206711.1 |
CG33249-RA (CG33249), mRNA |
| 0 |
0 |
1 |
3 |
12 |
NM_143372.1 |
CG9995-RA (htt), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_139415.1 |
CG13924-RA (CG13924), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_079751.3 |
CG31136-RA (Syx1A), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_143619.2 |
CG11522-RB, transcript variant B (RpL6), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170554.1 |
CG11522-RA, transcript variant A (RpL6), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136201.3 |
CG2614-RA (CG2614), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_164354.1 |
CG31973-RB, transcript variant B (CG31973), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001042861.1 |
CG31973-RC, transcript variant C (CG31973), mRNA |
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