NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 12030R-2

Symbol CG12030 Full Name CG12030

CG No CG12030 Old CG No CG12030

Synonyms CG12030

Accession No (Link to NCBI) NM_138200.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564 [ PubMed ID = 34239451 ] [ RRC reference ]

Mondal BC, Shim J, Evans CJ, Banerjee U.
Pvr expression regulators in equilibrium signal control and maintenance of Drosophila blood progenitors.
Elife (2014) 3 e03626 [ PubMed ID = 25201876 ] [ RRC reference ]

Sanders RD, Sefton JM, Moberg KH, Fridovich-Keil JL.
UDP-galactose 4' epimerase (GALE) is essential for development of Drosophila melanogaster.
Dis Model Mech (2010) 3(9-10) 628-38 [ PubMed ID = 20519568 ] [ RRC reference ]

Daenzer JM, Sanders RD, Hang D, Fridovich-Keil JL.
UDP-galactose 4'-epimerase activities toward UDP-Gal and UDP-GalNAc play different roles in the development of Drosophila melanogaster.
PLoS Genet (2012) 8(5) e1002721 [ PubMed ID = 22654673 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCACACGGTTCTGGAGATGCT

Primer Seq. 3'

aatctagaggtaccGCGAAACCACTGCCCAACGCT

Predicted Fragment Size

IR fragment full Seq

0001 CCACACGGTT CTGGAGATGC TCAATGCGGG CTACAACGTC ATCTGCGTGG ACAACCTGTG 
0061 CAACGCCTAC AGCAGCGGGG CCAAATTGCC GGAGGCCCTC AGCCGGGTGC AGGAAATCAC 
0121 CGGCAAGAAG GTCAACTTCT ATAGAGTGGA CATCACGGAC AGGGAGCAGG TGCGCTCCGT 
0181 CTTCCAGGAG CACAAAATCG ACATGGTGGC CCACTTTGCC GCCCTGAAGG CCGTGGGTGA 
0241 GTCCTGCCGC ATACCTTTGC AATACTACCA CAACAACATG ACCGGCACCA ATGTCCTGCT 
0301 GGAGGTGATG GCCGACAACA ATGTCTTCAA GTTCGTGTAC AGCTCCAGTG CCACCGTCTA 
0361 CGGTGAGCCA AAGTTCCTGC CCCGTGACCG AGGAGCATCC CCACGGGCAA ACTGTACATT 
0421 CGCCCCTACG GCAAGACTAA ATATTTCACC GAGGAGATTC CTCAAGGGAT CTGTGCCAAG 
0481 TCTGACAAGC GTTGGGCAGT GGTTTBGC

in silico PCR Fragment

0001 CCACACGGTT CTGGAGATGC TCAATGCGGG CTACAACGTC ATCTGCGTGG ACAACCTGTG 
0061 CAACGCCTAC AGCAGCGGGG CCAAACTGCC GGAGGCCCTC AGCCGGGTGC AGGAAATCAC 
0121 CGGCAAGAAG GTCAACTTCT ATAGAGTGGA CATCACGGAC AGGGAGCAGG TGCGCTCCGT 
0181 CTTCCAGGAG CACAAAATCG ACATGGTGGC CCACTTTGCC GCCCTGAAGG CCGTGGGTGA 
0241 GTCCTGCCGC ATACCTTTGC AGTACTACCA CAACAACATG ACCGGCACCA ATGTCCTGCT 
0301 GGAGGCGATG GCCGACAACA ATGTCTTCAA GTTCGTGTAC AGCTCCAGCG CCACCGTATA 
0361 CGGTGAGCCA AAGTTCCTG- CCCGTGACCG AGGAGCAT-C CCACGGGC-A ACTGTACA-T 
0421 CG-CCCTACG GCAAGACTAA ATATTTCACC GAGGAGATT- CTCAA-GGAT CTGTG-CAAG 
0481 TCTGACAAGC GTTGGGCAGT GGTTTCGC

Assemble Data


12030R-2.IR_full       1   CCACACGGTTCTGGAGATGCTCAATGCGGGCTACAACGTCATCTGCGTGGACAACCTGTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12030R-2.in silico     1   CCACACGGTTCTGGAGATGCTCAATGCGGGCTACAACGTCATCTGCGTGGACAACCTGTG 60


12030R-2.IR_full       61  CAACGCCTACAGCAGCGGGGCCAAATTGCCGGAGGCCCTCAGCCGGGTGCAGGAAATCAC 120
                           ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
12030R-2.in silico     61  CAACGCCTACAGCAGCGGGGCCAAACTGCCGGAGGCCCTCAGCCGGGTGCAGGAAATCAC 120


12030R-2.IR_full       121 CGGCAAGAAGGTCAACTTCTATAGAGTGGACATCACGGACAGGGAGCAGGTGCGCTCCGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12030R-2.in silico     121 CGGCAAGAAGGTCAACTTCTATAGAGTGGACATCACGGACAGGGAGCAGGTGCGCTCCGT 180


12030R-2.IR_full       181 CTTCCAGGAGCACAAAATCGACATGGTGGCCCACTTTGCCGCCCTGAAGGCCGTGGGTGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12030R-2.in silico     181 CTTCCAGGAGCACAAAATCGACATGGTGGCCCACTTTGCCGCCCTGAAGGCCGTGGGTGA 240


12030R-2.IR_full       241 GTCCTGCCGCATACCTTTGCAATACTACCACAACAACATGACCGGCACCAATGTCCTGCT 300
                           ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
12030R-2.in silico     241 GTCCTGCCGCATACCTTTGCAGTACTACCACAACAACATGACCGGCACCAATGTCCTGCT 300


12030R-2.IR_full       301 GGAGGTGATGGCCGACAACAATGTCTTCAAGTTCGTGTACAGCTCCAGTGCCACCGTCTA 360
                           ||||| |||||||||||||||||||||||||||||||||||||||||| |||||||| ||
12030R-2.in silico     301 GGAGGCGATGGCCGACAACAATGTCTTCAAGTTCGTGTACAGCTCCAGCGCCACCGTATA 360


12030R-2.IR_full       361 CGGTGAGCCAAAGTTCCTGCCCCGTGACCGAGGAGCATCCCCACGGGCAAACTGTACATT 420
                           ||||||||||||||||||| |||||||||||||||||| ||||||||| ||||||||| |
12030R-2.in silico     361 CGGTGAGCCAAAGTTCCTG-CCCGTGACCGAGGAGCAT-CCCACGGGC-AACTGTACA-T 420


12030R-2.IR_full       421 CGCCCCTACGGCAAGACTAAATATTTCACCGAGGAGATTCCTCAAGGGATCTGTGCCAAG 480
                           || |||||||||||||||||||||||||||||||||||| ||||| ||||||||| ||||
12030R-2.in silico     421 CG-CCCTACGGCAAGACTAAATATTTCACCGAGGAGATT-CTCAA-GGATCTGTG-CAAG 480


12030R-2.IR_full       481 TCTGACAAGCGTTGGGCAGTGGTTTBGC 508
                           ||||||||||||||||||||||||| ||
12030R-2.in silico     481 TCTGACAAGCGTTGGGCAGTGGTTTCGC 508

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	4	2	3	NM_138200.2	CG12030-RA (CG12030), mRNA
0	0	4	3	5	NM_135135.3	CG9098-RA, transcript variant A (CG9098), mRNA
0	0	4	2	4	NM_132956.1	CG9086-RA (CG9086), mRNA
0	0	3	12	3	NM_139365.2	CG18330-RA (Cct2), mRNA
0	0	3	3	10	NM_132111.2	CG4094-RA, transcript variant A (l(1)G0255), mRNA
0	0	3	3	10	NM_167081.2	CG4094-RB, transcript variant B (l(1)G0255), mRNA
0	0	2	5	3	NM_134498.2	CG14216-RA (CG14216), mRNA
0	0	2	3	9	NM_205889.1	CG31665-RB, transcript variant B (CG31665), mRNA
0	0	2	3	9	NM_164443.1	CG31665-RA, transcript variant A (CG31665), mRNA
0	0	2	3	6	NM_140390.2	CG10725-RB (CG10725), mRNA
0	0	2	2	5	NM_001038935.2	CG33989-RF, transcript variant F (pHCl), mRNA
0	0	2	2	4	NM_001038936.2	CG33989-RE, transcript variant E (pHCl), mRNA
0	0	2	2	4	NM_001038934.2	CG33989-RD, transcript variant D (pHCl), mRNA
0	0	2	2	4	NM_001038937.1	CG33989-RC, transcript variant C (pHCl), mRNA
0	0	2	2	3	NM_144017.1	CG18682-RA (CG18682), mRNA
0	0	1	6	3	NM_166281.1	CG5170-RE, transcript variant E (Dp1), mRNA
0	0	1	6	3	NM_166282.1	CG5170-RF, transcript variant F (Dp1), mRNA
0	0	1	6	3	NM_166280.1	CG5170-RD, transcript variant D (Dp1), mRNA
0	0	1	6	3	NM_206164.1	CG5170-RA, transcript variant A (Dp1), mRNA
0	0	1	6	3	NM_079057.2	CG5170-RC, transcript variant C (Dp1), mRNA
0	0	1	6	3	NM_206163.1	CG5170-RB, transcript variant B (Dp1), mRNA
0	0	1	6	3	NM_079581.2	CG6538-RA (TfIIFbeta), mRNA
0	0	1	4	2	NM_165935.1	CG8776-RB, transcript variant B (CG8776), mRNA
0	0	1	4	2	NM_165936.1	CG8776-RC, transcript variant C (CG8776), mRNA
0	0	1	4	2	NM_136964.2	CG8776-RE, transcript variant E (CG8776), mRNA
0	0	1	4	2	NM_165934.1	CG8776-RA, transcript variant A (CG8776), mRNA
0	0	1	4	2	NM_165937.1	CG8776-RD, transcript variant D (CG8776), mRNA
0	0	1	3	9	NM_135344.2	CG8486-RA, transcript variant A (CG8486), mRNA
0	0	1	3	9	NM_164795.2	CG8486-RB, transcript variant B (CG8486), mRNA
0	0	1	3	9	NM_001042881.1	CG8486-RC, transcript variant C (CG8486), mRNA

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