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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11929R-3 
 Symbol CG11929  Full Name CG11929 
 CG No CG11929  Old CG No CG11929 
 Synonyms CG11929 
 Accession No (Link to NCBI) NM_135005.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCCAAGGAAGACGAAAAGTGA 
 Primer Seq. 3' 
aatctagaggtaccCGCCGCCTTTGACTACGATTA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCCAAGGAAG ACGAAAAGTG ATTACTCCCT GACCCGCATC AATTTCAATT CCACTGATGC 
0061 TTCCATGGCC GATTCGCTGC CCACCGGGGC NACNGCAACT CGCCTACCTG CCGGAGCAAC 
0121 TTGCAGCTGT CGTTCCATTT CGGGACGCCC GAAACCCGCT GCCATCGCCT CGGAATCAAC 
0181 CCAAACCATA GCTTCCACCG AGGACATGGG CACTCAAACC GTCTTCCAAA CGGATCCCAA 
0241 TGAGGATTTC GATTGTGGCC AAGATGCATC CACCCAATCG ATGCTTAATA TGCATGATGT 
0301 AGAGACTCAA TCGATTCCAG TATCGTCCAA AAAACCTTAA CAGCATACAA ACGCAAACGG 
0361 ACTATGAAGA ACCAAAAACT GTCCGTTTTC CGTTGAAAAA ATATTCGAAG ACTGAGCATG 
0421 AAGAAAAAAG ACCTAGAAAA TCGAATACTT CATTGCGCAA ATGTTTTTTC AAGCGCCCAT 
0481 TAATCGTAGT CAAAGGCGGC G 
 in silico PCR Fragment 
0001 CCCAAGGAAG ACGAAAAGTG ATTACTCCCT GACCCGCATC AATTTCAATT CCACTGATGC 
0061 TTCCATGGCC GATTCGCTGC CCACCGGGGC GACTGCAACT CGCCTACCTG CCGGAGCAAC 
0121 TTGCAGCTGT CGTTCCATTT CGGGACGCCC GAAACCCGCT GCCATCGCCT CGGAATCAAC 
0181 CCAAACCATA GCTTCCACCG AGGACATGGG CACTCAAACC GTCTTCCAAA CGGATCCCAA 
0241 TGAGGATTTC GATTGTGGCC AAGATGCATC CACCCAATCG ATGCTTAATA TGCATGATGT 
0301 AGAGACTCAA TCGATTCCAG TATCGTCC-A AAAACCTTAA CAGCATACAA ACGCAAACGG 
0361 ACTATGAAGA ACCAAAAACT GTCCGTTTTC CGTTGAAAAA ATATTCGAAG ACTGAGCATG 
0421 AAGAAAAAAG ACCTAGAAAA TCGAATACTT CATTGCGCAA ATGTTTTTTC AAGCGCCCAT 
0481 TAATCGTAGT CAAAGGCGGC G 
 Assemble Data 

11929R-3.IR_full       1   CCCAAGGAAGACGAAAAGTGATTACTCCCTGACCCGCATCAATTTCAATTCCACTGATGC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11929R-3.in silico     1   CCCAAGGAAGACGAAAAGTGATTACTCCCTGACCCGCATCAATTTCAATTCCACTGATGC 60


11929R-3.IR_full       61  TTCCATGGCCGATTCGCTGCCCACCGGGGCNACNGCAACTCGCCTACCTGCCGGAGCAAC 120
                           |||||||||||||||||||||||||||||| || ||||||||||||||||||||||||||
11929R-3.in silico     61  TTCCATGGCCGATTCGCTGCCCACCGGGGCGACTGCAACTCGCCTACCTGCCGGAGCAAC 120


11929R-3.IR_full       121 TTGCAGCTGTCGTTCCATTTCGGGACGCCCGAAACCCGCTGCCATCGCCTCGGAATCAAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11929R-3.in silico     121 TTGCAGCTGTCGTTCCATTTCGGGACGCCCGAAACCCGCTGCCATCGCCTCGGAATCAAC 180


11929R-3.IR_full       181 CCAAACCATAGCTTCCACCGAGGACATGGGCACTCAAACCGTCTTCCAAACGGATCCCAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11929R-3.in silico     181 CCAAACCATAGCTTCCACCGAGGACATGGGCACTCAAACCGTCTTCCAAACGGATCCCAA 240


11929R-3.IR_full       241 TGAGGATTTCGATTGTGGCCAAGATGCATCCACCCAATCGATGCTTAATATGCATGATGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11929R-3.in silico     241 TGAGGATTTCGATTGTGGCCAAGATGCATCCACCCAATCGATGCTTAATATGCATGATGT 300


11929R-3.IR_full       301 AGAGACTCAATCGATTCCAGTATCGTCCAAAAAACCTTAACAGCATACAAACGCAAACGG 360
                           |||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
11929R-3.in silico     301 AGAGACTCAATCGATTCCAGTATCGTCC-AAAAACCTTAACAGCATACAAACGCAAACGG 360


11929R-3.IR_full       361 ACTATGAAGAACCAAAAACTGTCCGTTTTCCGTTGAAAAAATATTCGAAGACTGAGCATG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11929R-3.in silico     361 ACTATGAAGAACCAAAAACTGTCCGTTTTCCGTTGAAAAAATATTCGAAGACTGAGCATG 420


11929R-3.IR_full       421 AAGAAAAAAGACCTAGAAAATCGAATACTTCATTGCGCAAATGTTTTTTCAAGCGCCCAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11929R-3.in silico     421 AAGAAAAAAGACCTAGAAAATCGAATACTTCATTGCGCAAATGTTTTTTCAAGCGCCCAT 480


11929R-3.IR_full       481 TAATCGTAGTCAAAGGCGGCG 501
                           |||||||||||||||||||||
11929R-3.in silico     481 TAATCGTAGTCAAAGGCGGCG 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_135005.1  CG11929-RA (CG11929), mRNA 
0.2   1  3  2  2  NM_165846.2  CG8234-RB, transcript variant B (CG8234), mRNA 
0.2   1  3  2  2  NM_136850.2  CG8234-RA, transcript variant A (CG8234), mRNA 
0   0  2  2  4  NM_166433.1  CG30393-RA (CG30393), mRNA 
0   0  1  6  5  NM_057971.4  CG1584-RA (Orc6), mRNA 
0   0  1  4  6  NM_137462.3  CG5721-RA (CG5721), mRNA 
0   0  1  2  4  NM_130489.2  CG17896-RB, transcript variant B (CG17896), mRNA 
0   0  1  2  4  NM_166844.1  CG17896-RA, transcript variant A (CG17896), mRNA 
0   0  1  2  4  NM_057473.3  CG8987-RA (tam), mRNA 
0   0  0  5  5  NM_167015.2  CG6998-RC, transcript variant C (ctp), mRNA 
0   0  0  4  7  NM_170147.1  CG6798-RB, transcript variant B (nAcRbeta-96A), mRNA 
0   0  0  4  7  NM_079759.2  CG6798-RA, transcript variant A (nAcRbeta-96A), mRNA 
0   0  0  4  7  NM_080516.1  CG16757-RA (Spn), mRNA 
0   0  0  4  5  NM_079601.2  CG11502-RB, transcript variant B (svp), mRNA 
0   0  0  4  5  NM_169459.1  CG11502-RC, transcript variant C (svp), mRNA 
0   0  0  4  3  NM_001038916.1  CG17689-RB, transcript variant B (CG17689), mRNA 
0   0  0  4  3  NM_166500.1  CG30327-RA (CG30327), mRNA 
0   0  0  4  3  NM_140402.2  CG17689-RA, transcript variant A (CG17689), mRNA 
0   0  0  4  2  NM_001038832.1  CG40169-RB, transcript variant B (CC40169), mRNA 
0   0  0  4  2  NM_058067.2  CG6147-RA (Tsc1), mRNA 
0   0  0  4  2  NM_132627.1  CG4346-RA (rad), mRNA 
0   0  0  3  6  NM_132441.1  CG2174-RA, transcript variant A (Myo10A), mRNA 
0   0  0  3  6  NM_001042804.1  CG2174-RB, transcript variant B (Myo10A), mRNA 
0   0  0  3  5  NM_134948.1  CG8853-RA (CG8853), mRNA 
0   0  0  3  4  NM_164707.1  CG11199-RB, transcript variant B (Liprin-alpha), mRNA 
0   0  0  3  4  NM_135223.2  CG11199-RA, transcript variant A (Liprin-alpha), mRNA 
0   0  0  3  3  NM_141895.2  CG14740-RA (CG14740), mRNA 
0   0  0  3  3  NM_168179.1  CG32394-RA (CG32394), mRNA 
0   0  0  3  2  NM_132426.1  CG15209-RA (CG15209), mRNA 
0   0  0  3  2  NM_139844.2  CG8606-RA, transcript variant A (RhoGEF4), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
+- 
DB
+- 
DC
+ 
DM
+- 
VM
+- 
VB
 
Caspase-3 signal
N
++ 
DB
++ 
DC
++ 
DM
++ 
VM
++ 
VB
+ 
Dcompartment   Penetrance
+     

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