NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 11898R-2

Symbol CG11898 Full Name CG11898

CG No CG11898 Old CG No CG11898

Synonyms Dme_CG11898, CG11898

Accession No (Link to NCBI) NM_143422.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCGCCAGCCGAATCCAGTGA

Primer Seq. 3'

aatctagaggtaccGAGGAACTTCCGGTAGACGAG

Predicted Fragment Size

IR fragment full Seq

0001 AGCGCCAGCC GAATCCAGTG ACAAAGGCCA ACTTCTTTTC CAAATGGTTC TTTATCTGGA 
0061 CGCGCGAGAT TCTCGTCAAG GGACTGCAAC GGAATCTGGA TCCATCGGAT CTCTACGAGA 
0121 CCGAGCCCAG TCTCGAGAGC ACCCAGGTGT CCTCGTTCCT GCTTGGCCAC TGGGAGCAGG 
0181 AGCTCAAGCG GTCCAAGCCC AACGTCCTGC GCATGATATT NCAAGGCCTA CGGCTGGAGC 
0241 TTTGTGCCGG CGAGCATCAT GTACTCCATC ATGGCCATAG CAGTGCACAC AACGCAGCCC 
0301 TTGATGTTGG GCGGCTTGGT GTCCTTCTTC TCGGAGAGCA CGGGGAAGAT CACAAAGCAC 
0361 TCGGCCTACT TGTACGCCAT GGGCGTGGTG CTTTGCTCCC TCATCTCTGG ACTCTTCTTC 
0421 CACCCCTTTA TGAAATACCT GTTCAGAGTA GGCTCTCGCG TCCGTTTGGC CTGCGCGGGA 
0481 CTCGTCTACC GGAAGTTCCT C

in silico PCR Fragment

0001 AGCGCCAGCC GAATCCAGTG ACAAAGGCCA ACTTCTTTTC CAAATGGTTC TTTATCTGGA 
0061 CGCGCGAGAT TCTCGTCAAG GGACTGCAAC GGAATCTGGA TCCATCGGAT CTCTACGAGA 
0121 CCGAGCCCAG TCTCGAGAGC ACCCAGGTGT CCTCGTTCCT GCTTGGCCAC TGGGAGCAGG 
0181 AGCTCAAGCG GTCCAAGCCC AACGTCCTGC GCATGATATT -CAAGGCCTA CGGCTGGAGC 
0241 TTTGTGCCGG CGAGCATCGT GTACTCCATC ATGGCCATAG CAGTGCACAC AACGCAGCCC 
0301 TTGATGTTGG GCGGCTTGGT GTCCTTCTTC TCGGAGAGCA CGGGGAAGAT CACAAAGCAC 
0361 TCGGCCTACT TGTACGCCAT GGGCGTGGTG CTTTGCTCCC TCATCTCTGG ACTCTTCTTC 
0421 CACCCCTTTA TGAAATACCT GTTCAGAGTA GGCTCTCGCG TCCGTTTGGC CTGCGCGGGA 
0481 CTCGTCTACC GGAAGTTCCT C

Assemble Data


11898R-2.IR_full       1   AGCGCCAGCCGAATCCAGTGACAAAGGCCAACTTCTTTTCCAAATGGTTCTTTATCTGGA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11898R-2.in silico     1   AGCGCCAGCCGAATCCAGTGACAAAGGCCAACTTCTTTTCCAAATGGTTCTTTATCTGGA 60


11898R-2.IR_full       61  CGCGCGAGATTCTCGTCAAGGGACTGCAACGGAATCTGGATCCATCGGATCTCTACGAGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11898R-2.in silico     61  CGCGCGAGATTCTCGTCAAGGGACTGCAACGGAATCTGGATCCATCGGATCTCTACGAGA 120


11898R-2.IR_full       121 CCGAGCCCAGTCTCGAGAGCACCCAGGTGTCCTCGTTCCTGCTTGGCCACTGGGAGCAGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11898R-2.in silico     121 CCGAGCCCAGTCTCGAGAGCACCCAGGTGTCCTCGTTCCTGCTTGGCCACTGGGAGCAGG 180


11898R-2.IR_full       181 AGCTCAAGCGGTCCAAGCCCAACGTCCTGCGCATGATATTNCAAGGCCTACGGCTGGAGC 240
                           |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
11898R-2.in silico     181 AGCTCAAGCGGTCCAAGCCCAACGTCCTGCGCATGATATT-CAAGGCCTACGGCTGGAGC 240


11898R-2.IR_full       241 TTTGTGCCGGCGAGCATCATGTACTCCATCATGGCCATAGCAGTGCACACAACGCAGCCC 300
                           |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
11898R-2.in silico     241 TTTGTGCCGGCGAGCATCGTGTACTCCATCATGGCCATAGCAGTGCACACAACGCAGCCC 300


11898R-2.IR_full       301 TTGATGTTGGGCGGCTTGGTGTCCTTCTTCTCGGAGAGCACGGGGAAGATCACAAAGCAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11898R-2.in silico     301 TTGATGTTGGGCGGCTTGGTGTCCTTCTTCTCGGAGAGCACGGGGAAGATCACAAAGCAC 360


11898R-2.IR_full       361 TCGGCCTACTTGTACGCCATGGGCGTGGTGCTTTGCTCCCTCATCTCTGGACTCTTCTTC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11898R-2.in silico     361 TCGGCCTACTTGTACGCCATGGGCGTGGTGCTTTGCTCCCTCATCTCTGGACTCTTCTTC 420


11898R-2.IR_full       421 CACCCCTTTATGAAATACCTGTTCAGAGTAGGCTCTCGCGTCCGTTTGGCCTGCGCGGGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11898R-2.in silico     421 CACCCCTTTATGAAATACCTGTTCAGAGTAGGCTCTCGCGTCCGTTTGGCCTGCGCGGGA 480


11898R-2.IR_full       481 CTCGTCTACCGGAAGTTCCTC 501
                           |||||||||||||||||||||
11898R-2.in silico     481 CTCGTCTACCGGAAGTTCCTC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_143422.1	CG11898-RA (CG11898), mRNA
0	0	3	7	4	NM_137027.1	CG4744-RA (CG4744), mRNA
0	0	3	4	1	NM_164343.1	CG1090-RA, transcript variant A (CG1090), mRNA
0	0	3	4	1	NM_141202.2	CG1090-RB, transcript variant B (CG1090), mRNA
0	0	3	2	2	NM_001038966.1	CG33967-RA (CG33967), mRNA
0	0	1	4	2	NM_141580.1	CG8359-RA (CG8359), mRNA
0	0	1	3	6	NM_137805.1	CG11275-RA (CG11275), mRNA
0	0	1	3	4	NM_164385.1	CG3018-RB, transcript variant B (lwr), mRNA
0	0	1	3	4	NM_057630.3	CG3018-RA, transcript variant A (lwr), mRNA
0	0	1	3	3	NM_132169.1	CG9657-RA (CG9657), mRNA
0	0	1	3	2	NM_165196.1	CG31739-RA (CG31739), mRNA
0	0	1	3	2	NM_167324.1	CG32653-RA (CG32653), mRNA
0	0	1	2	5	NM_001043247.1	CG6535-RB (tefu), mRNA
0	0	1	2	4	NM_132997.2	CG8408-RA (CG8408), mRNA
0	0	1	2	3	NM_132844.2	CG8952-RA (CG8952), mRNA
0	0	1	2	2	NM_141072.2	CG7177-RA (CG7177), mRNA
0	0	0	9	7	NM_057853.2	CG2189-RA (Dfd), mRNA
0	0	0	7	5	NM_079270.1	CG4190-RA (Hsp67Bc), mRNA
0	0	0	6	3	NM_176728.1	CG33174-RA, transcript variant A (CG33174), mRNA
0	0	0	6	3	NM_176727.1	CG33174-RD, transcript variant D (CG33174), mRNA
0	0	0	5	6	NM_136450.2	CG2140-RB, transcript variant B (Cyt-b5), mRNA
0	0	0	5	5	NM_132831.1	CG8184-RB (CG8184), mRNA
0	0	0	5	5	NM_140079.1	CG3280-RA (CG3280), mRNA
0	0	0	5	4	NM_141734.2	CG11870-RA, transcript variant A (CG11870), mRNA
0	0	0	5	4	NM_136257.2	CG8677-RA (CG8677), mRNA
0	0	0	5	4	NM_206470.1	CG11870-RC, transcript variant C (CG11870), mRNA
0	0	0	5	4	NM_206469.1	CG11870-RD, transcript variant D (CG11870), mRNA
0	0	0	5	4	NM_169495.2	CG7771-RB, transcript variant B (sim), mRNA
0	0	0	5	4	NM_079616.2	CG7771-RA, transcript variant A (sim), mRNA
0	0	0	5	4	NM_169337.2	CG11870-RB, transcript variant B (CG11870), mRNA

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