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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11882R-4 
 Symbol CG11882  Full Name CG11882 
 CG No CG11882  Old CG No CG11882 
 Synonyms CG11882 
 Accession No (Link to NCBI) NM_143419.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees male lethal, female semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGTCCGACGAAGACGTCTTTG 
 Primer Seq. 3' 
aatctagaggtaccCTGCTCGAACTTTTCGGCCTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGTCCGACGA AGACGTCTTT GTGAAGCCCG ACTTGGACGA CGTGATCGAC GAGAATCGCG 
0061 CCGTGCTGTT TTCAATGCAG TCGGCAGATC TGCCGCCGCC TTGTGAGCTG CGCTTCCAGA 
0121 TCCCGAACCG CTTCAAAANT CGCCGCGCTC ACCCTGATTT GCAGCGCTCC CAAGGTGGAG 
0181 CTGTTCATTG GACCCTTGCA GGAGTACTGC GAGACCATCT ACGGCACTTG CGTCGACGAT 
0241 GAGGACCCAG AGGTTGCGGT GCGACCCTAT CGCTATGACA TGGAAATCGA CCGATCCGGG 
0301 ATAAATGACA TAAACCTGAA GATGCTCACC TCGGCCAGCG AGATCTGCGT TTATGGAGCC 
0361 CTGCTCCAGG TGGCGCCGAA TCCCAACGGC ATAACCACCC AGATGCCCAG GCCAATGGAT 
0421 GCGCAGCGAA TCCAGGAGCT GCTGCAAAGG GGTGGCACGA GTCCCTTGAA GAAGGAGGAG 
0481 CAGGCCGAAA AGTTCGAGCA G 
 in silico PCR Fragment 
0001 TGTCCGACGA AGACGTCTTT GTGAAGCCCG ACTTGGACGA CGTGATCGAC GAGAATCGCG 
0061 CCGTGCTGTT TTCCATGCAG TCGGCGGATC CGCCGCCGCC TTGTGAGCTG CGCTTCCAGA 
0121 TCCCGAACCG CTTCAAAA-T CGCCGCGCTC ACCCTGATTT GCAGCGCTCC CAAGGTGGAG 
0181 CTGTTCATTG GACCCTTGCA GGAGTACTGC GAGACCATCT ACGGCACTTG CGTCGACGAT 
0241 GAGGACCCAG AGGTTGCGGT GCGACCCTAT CGCTATGACA TGGAAATCGA CCGATCCGGG 
0301 ATAAATGACA TAAACCTGAA GATGCTCACC TCGGCCAGCG AGATCTGCGT TTACGGAGCC 
0361 CTGCTCCAGG TGGCGCCGAA TCCCAACGGC ATAACCACCC AGTTGCCCAG GCCAATGGAT 
0421 GCGCAGCGAA TCCAAGAGCT GCTGCAAAGG GGTGGCACGA GTCCCTTGAA GAAGGAGGAG 
0481 CAGGCCGAAA AGTTCGAGCA G 
 Assemble Data 

11882R-4.IR_full       1   TGTCCGACGAAGACGTCTTTGTGAAGCCCGACTTGGACGACGTGATCGACGAGAATCGCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11882R-4.in silico     1   TGTCCGACGAAGACGTCTTTGTGAAGCCCGACTTGGACGACGTGATCGACGAGAATCGCG 60


11882R-4.IR_full       61  CCGTGCTGTTTTCAATGCAGTCGGCAGATCTGCCGCCGCCTTGTGAGCTGCGCTTCCAGA 120
                           ||||||||||||| ||||||||||| |||| |||||||||||||||||||||||||||||
11882R-4.in silico     61  CCGTGCTGTTTTCCATGCAGTCGGCGGATCCGCCGCCGCCTTGTGAGCTGCGCTTCCAGA 120


11882R-4.IR_full       121 TCCCGAACCGCTTCAAAANTCGCCGCGCTCACCCTGATTTGCAGCGCTCCCAAGGTGGAG 180
                           |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
11882R-4.in silico     121 TCCCGAACCGCTTCAAAA-TCGCCGCGCTCACCCTGATTTGCAGCGCTCCCAAGGTGGAG 180


11882R-4.IR_full       181 CTGTTCATTGGACCCTTGCAGGAGTACTGCGAGACCATCTACGGCACTTGCGTCGACGAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11882R-4.in silico     181 CTGTTCATTGGACCCTTGCAGGAGTACTGCGAGACCATCTACGGCACTTGCGTCGACGAT 240


11882R-4.IR_full       241 GAGGACCCAGAGGTTGCGGTGCGACCCTATCGCTATGACATGGAAATCGACCGATCCGGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11882R-4.in silico     241 GAGGACCCAGAGGTTGCGGTGCGACCCTATCGCTATGACATGGAAATCGACCGATCCGGG 300


11882R-4.IR_full       301 ATAAATGACATAAACCTGAAGATGCTCACCTCGGCCAGCGAGATCTGCGTTTATGGAGCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
11882R-4.in silico     301 ATAAATGACATAAACCTGAAGATGCTCACCTCGGCCAGCGAGATCTGCGTTTACGGAGCC 360


11882R-4.IR_full       361 CTGCTCCAGGTGGCGCCGAATCCCAACGGCATAACCACCCAGATGCCCAGGCCAATGGAT 420
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
11882R-4.in silico     361 CTGCTCCAGGTGGCGCCGAATCCCAACGGCATAACCACCCAGTTGCCCAGGCCAATGGAT 420


11882R-4.IR_full       421 GCGCAGCGAATCCAGGAGCTGCTGCAAAGGGGTGGCACGAGTCCCTTGAAGAAGGAGGAG 480
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
11882R-4.in silico     421 GCGCAGCGAATCCAAGAGCTGCTGCAAAGGGGTGGCACGAGTCCCTTGAAGAAGGAGGAG 480


11882R-4.IR_full       481 CAGGCCGAAAAGTTCGAGCAG 501
                           |||||||||||||||||||||
11882R-4.in silico     481 CAGGCCGAAAAGTTCGAGCAG 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  5  NM_143419.1  CG11882-RA (CG11882), mRNA 
0   0  5  2  2  NM_142270.1  CG8927-RA, transcript variant A (CG8927), mRNA 
0   0  5  2  2  NM_001038967.1  CG8927-RB, transcript variant B (CG8927), mRNA 
0   0  3  4  3  NM_168091.1  CG32244-RB, transcript variant B (CG32244), mRNA 
0   0  3  4  3  NM_168092.1  CG32244-RC, transcript variant C (CG32244), mRNA 
0   0  3  2  3  NM_205997.1  CG15270-RB, transcript variant B (CG15270), mRNA 
0   0  3  2  3  NM_135894.2  CG15270-RA, transcript variant A (CG15270), mRNA 
0   0  2  4  16  NM_079817.2  CG1842-RA (Dhc98D), mRNA 
0   0  1  4  5  NM_136839.2  CG30023-RA (sprt), mRNA 
0   0  1  4  3  NM_168227.2  CG7462-RB, transcript variant B (Ank2), mRNA 
0   0  1  4  3  NM_139891.3  CG7462-RC, transcript variant C (Ank2), mRNA 
0   0  1  3  3  NM_142086.2  CG9649-RA (CG9649), mRNA 
0   0  1  3  2  NM_167275.1  CG32668-RA (CG32668), mRNA 
0   0  1  2  5  NM_140006.1  CG5751-RA (TrpA1), mRNA 
0   0  1  2  3  NM_137077.2  CG13350-RA (CG13350), mRNA 
0   0  1  2  2  NM_139589.1  CG14985-RA (CG14985), mRNA 
0   0  1  2  2  NM_001032065.1  CG17768-RA, transcript variant A (CG17768), mRNA 
0   0  1  2  2  NM_001032066.1  CG33677-RB, transcript variant B (CG33677), mRNA 
0   0  0  6  5  NM_169899.2  CG4257-RC, transcript variant C (Stat92E), mRNA 
0   0  0  6  5  NM_169900.2  CG4257-RB, transcript variant B (Stat92E), mRNA 
0   0  0  6  5  NM_001014643.1  CG4257-RG, transcript variant G (Stat92E), mRNA 
0   0  0  6  5  NM_206520.1  CG4257-RE, transcript variant E (Stat92E), mRNA 
0   0  0  6  5  NM_206521.1  CG4257-RF, transcript variant F (Stat92E), mRNA 
0   0  0  6  3  NM_080079.2  CG32120-RA (sens), mRNA 
0   0  0  5  5  NM_132858.2  CG9056-RA (CG9056), mRNA 
0   0  0  5  4  NM_139985.2  CG5989-RA (CG5989), mRNA 
0   0  0  5  4  NM_135082.2  CG6604-RA (H15), mRNA 
0   0  0  4  11  NM_143719.2  CG12396-RA (Nnp-1), mRNA 
0   0  0  4  8  NM_164409.2  CG4896-RC, transcript variant C (CG4896), mRNA 
0   0  0  4  8  NM_164410.2  CG4896-RA, transcript variant A (CG4896), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
+ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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