NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 11871R-1

Symbol CG11870 Full Name CG11870

CG No CG11870 Old CG No CG11871

Synonyms EMK/KIAA0537, CG11871, CG11870

Accession No (Link to NCBI) NM_141734.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Couderc JL, Richard G, Vachias C, Mirouse V.
Drosophila LKB1 is required for the assembly of the polarized actin structure that allows spermatid individualization.
PLoS One (2017) 12(8) e0182279 [ PubMed ID = 28767695 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCCTCACCCACCAGGACCATA

Primer Seq. 3'

aatctagaggtaccGCGGCCTTGGAGGCACTTTCA

Predicted Fragment Size

IR fragment full Seq

0001 CCCTCACCCA CCAGGACCAT AACCCCCGCC CAGAGTCCGG TGCAGACGAA GAGGAAACTG 
0061 CAGCCCACTG TTTCTACGGA GAATGCAGCC GGAACTACAG CCAAAAAGAA GGAGAAGCCG 
0121 GCCAATAGCT CGTTTGTGAT CAGCAAGGAT GGTGCACCAC TGACTGAAGC GCCACCGACC 
0181 ATCATCGAAN CCGCAAGCTA CGCTCATGGA GGCGGAGACG ATTGCCAATA TACCCGAGGA 
0241 GGTTCCTGTT CCCAGTTACT CCCAGAAAGA CATGCAAGCG GTGGGTGATA TTTGCGATCT 
0301 GCTGATGGGC ACCGCCACAA CTCCAGCCCC TGCACCACCG GCACCCACAC CTGTTGCTCG 
0361 CCAGCCCACC AGGGGAAAAC TGGATGCCGT TGTTGAAACT CCTGAGGAAA AGGATGCCAC 
0421 CAAAGTGATC AAGAAGTTTG TGAACAAGCA CAAGACCGCG GATCTGGTAA ATGCCATCAA 
0481 TGAAAGTGCC TCCAAGGCCG C

in silico PCR Fragment

0001 CCCTCACCCA CCAGGACCAT AACCCCCGCC CAGAGTCCGG TGCAGACGAA GAGGAAACTG 
0061 CAGCCCACTG TTTCTACGGA GAATGCAGCC GGAACTACAG CCAAAAAGAA GGAGAAGCCG 
0121 GCCAATAGCT CGTTTGTGAT CAGCAAGGAT GGTGCACCAC TGACTGAAGC GCCACCGACC 
0181 ATCATCGAA- CCGCAAGCTA CGCTCATGGA GGCGGAGACG ATTGCCAATA TACCCGAGGA 
0241 GGTTCCTGTT CCCAGTTACT CCCAGAAAGA CATGCAAGCG GTGGGTGATA TTTGCGATCT 
0301 GCTGATGGGC ACCGCCACAA CTCCAGCCCC TGCACCACCG GCACCCACAC CTGTTGCTCG 
0361 CCAGCCCACC AGGGGAAAAC TGGATGCCGT TGTTGAAACT CCTGAGGAAA AGGATGCCAC 
0421 CAAAGTGATC AAGAAGTTTG TGAACAAGCA CAAGACCGCG GATCTGGTAA ATGCCATCAA 
0481 TGAAAGTGCC TCCAAGGCCG C

Assemble Data


11871R-1.IR_full       1   CCCTCACCCACCAGGACCATAACCCCCGCCCAGAGTCCGGTGCAGACGAAGAGGAAACTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11871R-1.in silico     1   CCCTCACCCACCAGGACCATAACCCCCGCCCAGAGTCCGGTGCAGACGAAGAGGAAACTG 60


11871R-1.IR_full       61  CAGCCCACTGTTTCTACGGAGAATGCAGCCGGAACTACAGCCAAAAAGAAGGAGAAGCCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11871R-1.in silico     61  CAGCCCACTGTTTCTACGGAGAATGCAGCCGGAACTACAGCCAAAAAGAAGGAGAAGCCG 120


11871R-1.IR_full       121 GCCAATAGCTCGTTTGTGATCAGCAAGGATGGTGCACCACTGACTGAAGCGCCACCGACC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11871R-1.in silico     121 GCCAATAGCTCGTTTGTGATCAGCAAGGATGGTGCACCACTGACTGAAGCGCCACCGACC 180


11871R-1.IR_full       181 ATCATCGAANCCGCAAGCTACGCTCATGGAGGCGGAGACGATTGCCAATATACCCGAGGA 240
                           ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
11871R-1.in silico     181 ATCATCGAA-CCGCAAGCTACGCTCATGGAGGCGGAGACGATTGCCAATATACCCGAGGA 240


11871R-1.IR_full       241 GGTTCCTGTTCCCAGTTACTCCCAGAAAGACATGCAAGCGGTGGGTGATATTTGCGATCT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11871R-1.in silico     241 GGTTCCTGTTCCCAGTTACTCCCAGAAAGACATGCAAGCGGTGGGTGATATTTGCGATCT 300


11871R-1.IR_full       301 GCTGATGGGCACCGCCACAACTCCAGCCCCTGCACCACCGGCACCCACACCTGTTGCTCG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11871R-1.in silico     301 GCTGATGGGCACCGCCACAACTCCAGCCCCTGCACCACCGGCACCCACACCTGTTGCTCG 360


11871R-1.IR_full       361 CCAGCCCACCAGGGGAAAACTGGATGCCGTTGTTGAAACTCCTGAGGAAAAGGATGCCAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11871R-1.in silico     361 CCAGCCCACCAGGGGAAAACTGGATGCCGTTGTTGAAACTCCTGAGGAAAAGGATGCCAC 420


11871R-1.IR_full       421 CAAAGTGATCAAGAAGTTTGTGAACAAGCACAAGACCGCGGATCTGGTAAATGCCATCAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11871R-1.in silico     421 CAAAGTGATCAAGAAGTTTGTGAACAAGCACAAGACCGCGGATCTGGTAAATGCCATCAA 480


11871R-1.IR_full       481 TGAAAGTGCCTCCAAGGCCGC 501
                           |||||||||||||||||||||
11871R-1.in silico     481 TGAAAGTGCCTCCAAGGCCGC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_206469.1	CG11870-RD, transcript variant D (CG11870), mRNA
100	482	0	0	1	NM_169337.2	CG11870-RB, transcript variant B (CG11870), mRNA
100	482	0	0	0	NM_206470.1	CG11870-RC, transcript variant C (CG11870), mRNA
100	482	0	0	0	NM_141734.2	CG11870-RA, transcript variant A (CG11870), mRNA
1.03	5	2	2	3	NM_165522.1	CG11112-RB, transcript variant B (CG11112), mRNA
0.62	3	3	4	19	NM_139645.2	CG15021-RA (CG15021), mRNA
0.2	1	2	2	2	NM_142712.1	CG7907-RA (CG7907), mRNA
0	0	3	5	6	NM_167143.1	CG32858-RB, transcript variant B (sn), mRNA
0	0	3	5	6	NM_167142.1	CG32858-RA, transcript variant A (sn), mRNA
0	0	2	6	6	NM_167757.1	CG14476-RA, transcript variant A (CG14476), mRNA
0	0	2	6	6	NM_167759.1	CG14476-RD, transcript variant D (CG14476), mRNA
0	0	2	6	6	NM_167760.1	CG14476-RE, transcript variant E (CG14476), mRNA
0	0	2	6	6	NM_167758.1	CG14476-RC, transcript variant C (CG14476), mRNA
0	0	2	6	6	NM_143888.4	CG14476-RB, transcript variant B (CG14476), mRNA
0	0	2	4	5	NM_141657.2	CG8273-RA (CG8273), mRNA
0	0	2	2	4	NM_166937.1	CG2841-RA, transcript variant A (ptr), mRNA
0	0	2	2	4	NM_080022.2	CG2841-RB, transcript variant B (ptr), mRNA
0	0	2	2	4	NM_166938.1	CG2841-RC, transcript variant C (ptr), mRNA
0	0	2	2	3	NM_142497.2	CG18208-RA (CG18208), mRNA
0	0	1	5	6	NM_079859.2	CG1438-RA (Cyp4c3), mRNA
0	0	1	5	6	NM_135644.2	CG4713-RA (CG4713), mRNA
0	0	1	4	10	NM_166934.1	CG3191-RA (CG3191), mRNA
0	0	1	4	7	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
0	0	1	4	7	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
0	0	1	4	5	NM_134527.1	CG15322-RA (CG15322), mRNA
0	0	1	3	6	NM_139517.1	CG14956-RA (CG14956), mRNA
0	0	1	3	5	NM_001014500.1	CG33558-RA (CG33558), mRNA
0	0	1	3	4	NM_143241.1	CG5484-RC, transcript variant C (CG5484), mRNA
0	0	1	3	4	NM_170284.1	CG5484-RA, transcript variant A (CG5484), mRNA
0	0	1	3	4	NM_170285.1	CG5484-RB, transcript variant B (CG5484), mRNA

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