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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11870R-2 
 Symbol CG11870  Full Name CG11870 
 CG No CG11870  Old CG No CG11870 
 Synonyms EMK/KIAA0537, CG11871, CG11870 
 Accession No (Link to NCBI) NM_141734.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGAGCTCAGTGCATCATCCC 
 Primer Seq. 3' 
aatctagaggtaccGGCTCGTAGTAATCACCCTGG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGAGCTCAG TGCATCATCC CAACATCATC CACATCTACG AAGTATTTGA GAATCGTGAG 
0061 AAAATGGTGC TAGTCATGGA ATTTGCCGCT GGCGGCGAGC ATCTACGACT ATCTGTCTGA 
0121 AAGGAAGGTT CTCACCGAGG AGGAGGCGAG ACGCATCTTC CGCCAGGTGG CCACCGCCGT 
0181 CTACTACTGT CACAAGCACA AGATCTGCCA TCGCGATCTC AAGCTGGAGA ACATCCTGCT 
0241 GGACGAGAAG GGCAATGCTA AGATTGCTGA TTTTGGGTTG TCGAATGTGT TTGATGACCA 
0301 GCGACTGCTG GGCACCTTTT GCGGTTCCCC ACTCTATGCC TCGCCGGAAA TTGTGGAAGG 
0361 CACACCATAC CAGGGACCCG AGGTGGACTG CTGGTCACTG GGCGTGCTGC TCTACACGCT 
0421 GGTCTACGGC TCTATGCCCT TCGATGGGTC CAATTTCAAG CGACTGGTGA AGCAGATCAG 
0481 CCANGGGTGA TTACTACGAG CC 
 in silico PCR Fragment 
0001 ATGAGCTCAG TGCATCATCC CAACATCATC CACATCTACG AAGTATTTGA GAATCGTGAG 
0061 AAAATGGTGC TAGTCATGGA ATTTGCCGCT GGCGGCGAGC -TCTACGACT ATCTGTCTGA 
0121 AAGGAAGGTT CTCACCGAGG AGGAGGCGAG ACGCATCTTC CGCCAGGTGG CCACCGCCGT 
0181 CTACTACTGT CACAAGCACA AGATCTGCCA TCGCGATCTC AAGCTGGAGA ACATCCTGCT 
0241 GGACGAGAAG GGCAATGCTA AGATTGCTGA TTTTGGGTTG TCGAATGTGT TTGATGACCA 
0301 GCGACTGCTG GGCACCTTTT GCGGTTCCCC ACTCTATGCC TCGCCGGAAA TTGTGGAAGG 
0361 CACACCATAC CAGGGACCCG AGGTGGACTG CTGGTCACTG GGCGTGCTGC TCTACACGCT 
0421 GGTCTACGGC TCTATGCCCT TCGATGGGTC CAATTTCAAG CGACTGGTGA AGCAGATCAG 
0481 CCA-GGGTGA TTACTACGAG CC 
 Assemble Data 

11870R-2.IR_full       1   ATGAGCTCAGTGCATCATCCCAACATCATCCACATCTACGAAGTATTTGAGAATCGTGAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11870R-2.in silico     1   ATGAGCTCAGTGCATCATCCCAACATCATCCACATCTACGAAGTATTTGAGAATCGTGAG 60


11870R-2.IR_full       61  AAAATGGTGCTAGTCATGGAATTTGCCGCTGGCGGCGAGCATCTACGACTATCTGTCTGA 120
                           |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
11870R-2.in silico     61  AAAATGGTGCTAGTCATGGAATTTGCCGCTGGCGGCGAGC-TCTACGACTATCTGTCTGA 120


11870R-2.IR_full       121 AAGGAAGGTTCTCACCGAGGAGGAGGCGAGACGCATCTTCCGCCAGGTGGCCACCGCCGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11870R-2.in silico     121 AAGGAAGGTTCTCACCGAGGAGGAGGCGAGACGCATCTTCCGCCAGGTGGCCACCGCCGT 180


11870R-2.IR_full       181 CTACTACTGTCACAAGCACAAGATCTGCCATCGCGATCTCAAGCTGGAGAACATCCTGCT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11870R-2.in silico     181 CTACTACTGTCACAAGCACAAGATCTGCCATCGCGATCTCAAGCTGGAGAACATCCTGCT 240


11870R-2.IR_full       241 GGACGAGAAGGGCAATGCTAAGATTGCTGATTTTGGGTTGTCGAATGTGTTTGATGACCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11870R-2.in silico     241 GGACGAGAAGGGCAATGCTAAGATTGCTGATTTTGGGTTGTCGAATGTGTTTGATGACCA 300


11870R-2.IR_full       301 GCGACTGCTGGGCACCTTTTGCGGTTCCCCACTCTATGCCTCGCCGGAAATTGTGGAAGG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11870R-2.in silico     301 GCGACTGCTGGGCACCTTTTGCGGTTCCCCACTCTATGCCTCGCCGGAAATTGTGGAAGG 360


11870R-2.IR_full       361 CACACCATACCAGGGACCCGAGGTGGACTGCTGGTCACTGGGCGTGCTGCTCTACACGCT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11870R-2.in silico     361 CACACCATACCAGGGACCCGAGGTGGACTGCTGGTCACTGGGCGTGCTGCTCTACACGCT 420


11870R-2.IR_full       421 GGTCTACGGCTCTATGCCCTTCGATGGGTCCAATTTCAAGCGACTGGTGAAGCAGATCAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11870R-2.in silico     421 GGTCTACGGCTCTATGCCCTTCGATGGGTCCAATTTCAAGCGACTGGTGAAGCAGATCAG 480


11870R-2.IR_full       481 CCANGGGTGATTACTACGAGCC 502
                           ||| ||||||||||||||||||
11870R-2.in silico     481 CCA-GGGTGATTACTACGAGCC 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_206469.1  CG11870-RD, transcript variant D (CG11870), mRNA 
100   482  0  0  0  NM_169337.2  CG11870-RB, transcript variant B (CG11870), mRNA 
100   482  0  0  0  NM_206470.1  CG11870-RC, transcript variant C (CG11870), mRNA 
100   482  0  0  0  NM_141734.2  CG11870-RA, transcript variant A (CG11870), mRNA 
0.41   2  3  4  4  NM_176751.1  CG8557-RB, transcript variant B (CG8557), mRNA 
0.41   2  3  4  4  NM_132990.2  CG8557-RA, transcript variant A (CG8557), mRNA 
0.2   1  4  3  2  NM_137189.2  CG8166-RA (unc-5), mRNA 
0   0  6  4  13  NM_176688.1  CG3086-RD, transcript variant D (MAPk-Ak2), mRNA 
0   0  6  4  13  NM_080030.3  CG3086-RB, transcript variant B (MAPk-Ak2), mRNA 
0   0  6  4  13  NM_167050.1  CG3086-RC, transcript variant C (MAPk-Ak2), mRNA 
0   0  6  3  2  NM_080141.2  CG10120-RB, transcript variant B (Men), mRNA 
0   0  6  3  2  NM_169479.1  CG10120-RA, transcript variant A (Men), mRNA 
0   0  5  2  1  NM_175947.1  CG33128-RA (CG33128), mRNA 
0   0  4  4  5  NM_176386.1  CG11489-RC, transcript variant C (CG11489), mRNA 
0   0  4  4  5  NM_141130.3  CG11489-RB, transcript variant B (CG11489), mRNA 
0   0  3  8  10  NM_167794.1  CG1210-RE, transcript variant E (Pk61C), mRNA 
0   0  3  8  10  NM_167793.1  CG1210-RB, transcript variant B (Pk61C), mRNA 
0   0  3  8  10  NM_167795.1  CG1210-RG, transcript variant G (Pk61C), mRNA 
0   0  3  8  10  NM_167789.1  CG1210-RC, transcript variant C (Pk61C), mRNA 
0   0  3  8  10  NM_167791.1  CG1210-RF, transcript variant F (Pk61C), mRNA 
0   0  3  8  10  NM_167792.1  CG1210-RH, transcript variant H (Pk61C), mRNA 
0   0  3  8  10  NM_080382.2  CG1210-RA, transcript variant A (Pk61C), mRNA 
0   0  3  8  10  NM_167790.1  CG1210-RD, transcript variant D (Pk61C), mRNA 
0   0  3  4  3  NM_135790.2  CG31729-RB, transcript variant B (CG31729), mRNA 
0   0  3  4  3  NM_165038.1  CG31729-RA, transcript variant A (CG31729), mRNA 
0   0  2  8  7  NM_141611.1  CG9746-RA (CG9746), mRNA 
0   0  2  8  4  NM_134925.2  CG8838-RA (CG8838), mRNA 
0   0  2  7  18  NM_079821.2  CG1954-RA (Pkc98E), mRNA 
0   0  2  6  4  NM_137519.2  CG15087-RA (CG15087), mRNA 
0   0  2  6  2  NM_142780.2  CG13855-RA (CG13855), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
+- 
DB
+- 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
++ 
DM
+ 
VM
+ 
VB
 
Dcompartment   Penetrance
     

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