NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 11842R-1

Symbol CG11842 Full Name CG11842

CG No CG11842 Old CG No CG11842

Synonyms SP68, CG11842

Accession No (Link to NCBI) NM_143405.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal

Map Viewer

Reference
[Please submit your publication] Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.
Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.
Curr Biol (2006) 16(8) 808-13 [ PubMed ID = 16631589 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGAGCAGCGCGATCTTCGCC

Primer Seq. 3'

aatctagaggtaccCGTTGTAAATCGCCGGGTAGC

Predicted Fragment Size

IR fragment full Seq

0001 ATGAGCAGCG CGATCTTCgN CAAGATTGTC CAGCTGGGAG CAGTACTGCT GGTTTCATTC 
0061 GTCGCATGGG CGTCAGCTCA GGATCCGGAT ATAGCCAGGA CGTGCACTGC CTACAAGAAA 
0121 AGCGTTTGGA GAGGAGACGT CCGAGTTCAG CTTTCTGATC GAGAACGCTC CGATTATCTA 
0181 CAAGACCCTG GACAAATGCA CTAGCTATGC GCGCGCTCAT CATCGGCGGA GGACCCGCTG 
0241 TGCCCAAGGA GTTNCCCGCA TGCTGCGCGC CTGGGACACA AAGATGAGAA TGGCGAGGTG 
0301 GAATGGTTCT GTGGCGGAAC ACTTATCAGC GACAGACATG TgNTCACCGC AGCGCATTGC 
0361 CACTACTCAC CACAAGGATC AGTGAACATC GCGCGCCTCG GGGACCTGGA ATNCGACACC 
0421 AACAACGACG ATGCGGATCC GGAGGACTTC GATGTGAAGG ACTTCACCGC GCACCCCGAA 
0481 TTTAGCTACC CGGCGATTTA CAACG

in silico PCR Fragment

0001 ATGAGCAGCG CGATCTTCGC CAAGATTGTC CAGCTGGGAG CAGTATTGCT GGTTTCATTC 
0061 GTCGCATGGG CGTCAGCTCA GGATTCGGAT ATAGCCAGGA CGTGCACTGC CTACAAGAGA 
0121 AGCGTTTGG- GAGGAGACGT CCGAGTTCAG CTTTCTGATC GAGAACGCTC CGATTATCTA 
0181 CAAGACCCTG GACAAATGCA CTAGCTATGC GC-CGCTCAT CATCGGCGGA GGACCCGCCG 
0241 TGCCCAAGGA GTT-CCCGCA TGCTGCGCGC CTGGGACACA AAGATGAGAA TGGCGAGGTG 
0301 GAATGGTTCT GTGGCGGAAC ACTTATCAGC GACAGACATG TGCTCACCGC AGCGCATTGC 
0361 CACTACTCAC CACAAGGATC AGTGAACATC GCGCGCCTCG GGGACCTGGA ATTCGACACC 
0421 AACAACGACG ATGCGGATCC GGAGGACTTC GATGTGAAGG ACTTCACCGC GCACCCCGAA 
0481 TTTAGCTACC CGGCGATTTA CAACG

Assemble Data


11842R-1.IR_full       1   ATGAGCAGCGCGATCTTCGNCAAGATTGTCCAGCTGGGAGCAGTACTGCTGGTTTCATTC 60
                           ||||||||||||||||||| ||||||||||||||||||||||||| ||||||||||||||
11842R-1.in silico     1   ATGAGCAGCGCGATCTTCGCCAAGATTGTCCAGCTGGGAGCAGTATTGCTGGTTTCATTC 60


11842R-1.IR_full       61  GTCGCATGGGCGTCAGCTCAGGATCCGGATATAGCCAGGACGTGCACTGCCTACAAGAAA 120
                           |||||||||||||||||||||||| ||||||||||||||||||||||||||||||||| |
11842R-1.in silico     61  GTCGCATGGGCGTCAGCTCAGGATTCGGATATAGCCAGGACGTGCACTGCCTACAAGAGA 120


11842R-1.IR_full       121 AGCGTTTGGAGAGGAGACGTCCGAGTTCAGCTTTCTGATCGAGAACGCTCCGATTATCTA 180
                           ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
11842R-1.in silico     121 AGCGTTTGG-GAGGAGACGTCCGAGTTCAGCTTTCTGATCGAGAACGCTCCGATTATCTA 180


11842R-1.IR_full       181 CAAGACCCTGGACAAATGCACTAGCTATGCGCGCGCTCATCATCGGCGGAGGACCCGCTG 240
                           |||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| |
11842R-1.in silico     181 CAAGACCCTGGACAAATGCACTAGCTATGCGC-CGCTCATCATCGGCGGAGGACCCGCCG 240


11842R-1.IR_full       241 TGCCCAAGGAGTTNCCCGCATGCTGCGCGCCTGGGACACAAAGATGAGAATGGCGAGGTG 300
                           ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
11842R-1.in silico     241 TGCCCAAGGAGTT-CCCGCATGCTGCGCGCCTGGGACACAAAGATGAGAATGGCGAGGTG 300


11842R-1.IR_full       301 GAATGGTTCTGTGGCGGAACACTTATCAGCGACAGACATGTGNTCACCGCAGCGCATTGC 360
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
11842R-1.in silico     301 GAATGGTTCTGTGGCGGAACACTTATCAGCGACAGACATGTGCTCACCGCAGCGCATTGC 360


11842R-1.IR_full       361 CACTACTCACCACAAGGATCAGTGAACATCGCGCGCCTCGGGGACCTGGAATNCGACACC 420
                           |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
11842R-1.in silico     361 CACTACTCACCACAAGGATCAGTGAACATCGCGCGCCTCGGGGACCTGGAATTCGACACC 420


11842R-1.IR_full       421 AACAACGACGATGCGGATCCGGAGGACTTCGATGTGAAGGACTTCACCGCGCACCCCGAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11842R-1.in silico     421 AACAACGACGATGCGGATCCGGAGGACTTCGATGTGAAGGACTTCACCGCGCACCCCGAA 480


11842R-1.IR_full       481 TTTAGCTACCCGGCGATTTACAACG 505
                           |||||||||||||||||||||||||
11842R-1.in silico     481 TTTAGCTACCCGGCGATTTACAACG 505

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	484	0	0	0	NM_143405.1	CG11842-RA (CG11842), mRNA
0.2	1	3	3	6	NM_135154.1	CG9227-RA (tectonic), mRNA
0	0	2	6	2	NM_137151.2	CG10212-RA (SMC2), mRNA
0	0	2	4	2	NM_176680.2	CG33082-RA (CG33082), mRNA
0	0	2	4	2	NM_143728.2	CG14630-RA (CG14630), mRNA
0	0	2	3	8	NM_167164.2	CG12737-RB, transcript variant B (Crag), mRNA
0	0	2	3	8	NM_080341.2	CG12737-RA, transcript variant A (Crag), mRNA
0	0	2	3	3	NM_079363.3	CG7450-RA, transcript variant A (CrebA), mRNA
0	0	2	3	3	NM_206374.1	CG7450-RB, transcript variant B (CrebA), mRNA
0	0	2	2	2	NM_057406.3	CG6222-RA (su(s)), mRNA
0	0	1	7	2	NM_136773.1	CG12343-RA (CG12343), mRNA
0	0	1	6	3	NM_137219.2	CG8256-RC, transcript variant C (l(2)k05713), mRNA
0	0	1	6	3	NM_166119.1	CG8256-RB, transcript variant B (l(2)k05713), mRNA
0	0	1	6	3	NM_166118.1	CG8256-RA, transcript variant A (l(2)k05713), mRNA
0	0	1	6	2	NR_001738.1	CR30082, mRNA
0	0	1	5	10	NM_078604.3	CG32592-RA (hiw), mRNA
0	0	1	5	4	NM_057278.3	CG7727-RA (Appl), mRNA
0	0	1	3	8	NM_143404.1	CG11841-RA (CG11841), mRNA
0	0	1	3	2	NM_169602.1	CG7390-RB, transcript variant B (smp-30), mRNA
0	0	1	3	2	NM_130672.2	CG2712-RA (CG2712), mRNA
0	0	1	3	2	NM_079629.2	CG7390-RA, transcript variant A (smp-30), mRNA
0	0	1	2	8	NM_176225.1	CG15086-RB, transcript variant B (CG15086), mRNA
0	0	1	2	8	NM_176226.1	CG15086-RC, transcript variant C (CG15086), mRNA
0	0	1	2	8	NM_176227.1	CG15086-RD, transcript variant D (CG15086), mRNA
0	0	1	2	8	NM_137513.2	CG15086-RA, transcript variant A (CG15086), mRNA
0	0	1	2	8	NM_137794.2	CG11073-RA (CG11073), mRNA
0	0	1	2	6	NM_166592.1	CG30417-RA (CG30417), mRNA
0	0	1	2	6	NM_170639.1	CG17122-RA (CG17122), mRNA
0	0	1	2	3	NM_169258.1	CG9755-RA, transcript variant A (pum), mRNA
0	0	1	2	3	NM_169259.1	CG9755-RD, transcript variant D (pum), mRNA

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