NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 11757R-1

Symbol CG34348 Full Name -

CG No CG34348 Old CG No CG11757

Synonyms FBgn0085377, CG11757, CG15197, CG34348

Accession No (Link to NCBI) NM_132478.1

Inserted Chr. ll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment balanced with SM6a, Cy

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCCATTTGGGATGCACATGCT

Primer Seq. 3'

aatctagaggtaccACCGGTATGCGCACCTTATTG

Predicted Fragment Size

IR fragment full Seq

0001 GCCATTTGGG ATGCACATGC TCGGATCTAT CATGGCTACG AGGTGATCGG ATTGGAGAAT 
0061 GTGCCACAGG AGGGACCGGC GCTGATTGTC TACTATCATG GCGCCATACC CATCGATATG 
0121 TACTACTTGA ATTCGCGAAT GTTGCTGCAG CGCGAGCGTT TGATCTACAC GATCGGCGAT 
0181 CGATTTCTGT TCAAGCTGCC CGGCTGGGGC ACCATTTCGG AGGCATTCCA CGTCAGTCCC 
0241 GGCACGGTGC AGTCGTGCGT TAGCATTTTA CGGGATGGCA ATCTGCTGGC CATTTCGCCG 
0301 GGCGGCGTTT ATGAGGCACA GTTCGGTGAT CACTACTACG AGCTGTTGTG GCGCAATCGT 
0361 GTCGGTTTCG CCAAGGTGGC CATTGAGGCA AAGGCGCCCA TCATTCCCTG CTTTACGCAG 
0421 AATCTGCGCG AGGGCTTCCG TCAGGTGGGC ATCTTTCGGA CGTTCTTCAT GCGACTGTAC 
0481 AATAAGGTGC GCATACCAGT GGT

in silico PCR Fragment

0001 GCCATTTGGG ATGCACATGC TCGGATCTAT CATGGCTACG AGGTGATCGG ATTGGAGAAT 
0061 GTGCCACAGG AGGGACCGGC GCTGATTGTC TACTATCATG GCGCCATACC CATCGATATG 
0121 TACTACTTGA ATTCGCGAAT GTTGCTGCAG CGCGAGCGTT TGATCTACAC GATCGGCGAT 
0181 CGATTTCTGT TCAAGCTGCC CGGCTGGGGC ACCATCTCTG AGGCATTCCA CGTCAGTCCC 
0241 GGCACGGTGC AGTCGTGCGT TAGCATTTTG CGGGATGGCA ATCTGCTGGC CATTTCGCCG 
0301 GGCGGCGTTT ATGAGGCACA GTTCGGTGAT CACTACTACG AGCTGTTGTG GCGCAATCGT 
0361 GTCGGTTTCG CCAAGGTGGC CATCGAGGCA AAGGCGCCCA TCATTCCCTG CTTTACGCAG 
0421 AATCTGCGCG AGGGCTTCCG TCAGGTGGGC ATCTTTCGGA CGTTCTTCAT GCGACTGTAC 
0481 AATAAGGTGC GCATACC--- GGT

Assemble Data


11757R-1.IR_full       1   GCCATTTGGGATGCACATGCTCGGATCTATCATGGCTACGAGGTGATCGGATTGGAGAAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11757R-1.in silico     1   GCCATTTGGGATGCACATGCTCGGATCTATCATGGCTACGAGGTGATCGGATTGGAGAAT 60


11757R-1.IR_full       61  GTGCCACAGGAGGGACCGGCGCTGATTGTCTACTATCATGGCGCCATACCCATCGATATG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11757R-1.in silico     61  GTGCCACAGGAGGGACCGGCGCTGATTGTCTACTATCATGGCGCCATACCCATCGATATG 120


11757R-1.IR_full       121 TACTACTTGAATTCGCGAATGTTGCTGCAGCGCGAGCGTTTGATCTACACGATCGGCGAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11757R-1.in silico     121 TACTACTTGAATTCGCGAATGTTGCTGCAGCGCGAGCGTTTGATCTACACGATCGGCGAT 180


11757R-1.IR_full       181 CGATTTCTGTTCAAGCTGCCCGGCTGGGGCACCATTTCGGAGGCATTCCACGTCAGTCCC 240
                           ||||||||||||||||||||||||||||||||||| || |||||||||||||||||||||
11757R-1.in silico     181 CGATTTCTGTTCAAGCTGCCCGGCTGGGGCACCATCTCTGAGGCATTCCACGTCAGTCCC 240


11757R-1.IR_full       241 GGCACGGTGCAGTCGTGCGTTAGCATTTTACGGGATGGCAATCTGCTGGCCATTTCGCCG 300
                           ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
11757R-1.in silico     241 GGCACGGTGCAGTCGTGCGTTAGCATTTTGCGGGATGGCAATCTGCTGGCCATTTCGCCG 300


11757R-1.IR_full       301 GGCGGCGTTTATGAGGCACAGTTCGGTGATCACTACTACGAGCTGTTGTGGCGCAATCGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11757R-1.in silico     301 GGCGGCGTTTATGAGGCACAGTTCGGTGATCACTACTACGAGCTGTTGTGGCGCAATCGT 360


11757R-1.IR_full       361 GTCGGTTTCGCCAAGGTGGCCATTGAGGCAAAGGCGCCCATCATTCCCTGCTTTACGCAG 420
                           ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
11757R-1.in silico     361 GTCGGTTTCGCCAAGGTGGCCATCGAGGCAAAGGCGCCCATCATTCCCTGCTTTACGCAG 420


11757R-1.IR_full       421 AATCTGCGCGAGGGCTTCCGTCAGGTGGGCATCTTTCGGACGTTCTTCATGCGACTGTAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11757R-1.in silico     421 AATCTGCGCGAGGGCTTCCGTCAGGTGGGCATCTTTCGGACGTTCTTCATGCGACTGTAC 480


11757R-1.IR_full       481 AATAAGGTGCGCATACCAGTGGT 503
                           |||||||||||||||||   |||
11757R-1.in silico     481 AATAAGGTGCGCATACC---GGT 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_132478.1	CG11757-RA (CG11757), mRNA
0	0	3	4	2	NM_079159.2	CG1004-RA (rho), mRNA
0	0	2	4	6	NM_137787.1	CG13501-RA (CG13501), mRNA
0	0	2	3	2	NM_001032080.1	CG33695-RA, transcript variant A (CG33695), mRNA
0	0	2	3	2	NM_001032076.1	CG33694-RA, transcript variant A (cana), mRNA
0	0	1	9	2	NM_141603.1	CG11983-RA (CG11983), mRNA
0	0	1	7	4	NM_080341.2	CG12737-RA, transcript variant A (Crag), mRNA
0	0	1	7	3	NM_167164.2	CG12737-RB, transcript variant B (Crag), mRNA
0	0	1	5	2	NM_057450.3	CG9704-RB, transcript variant B (Nrt), mRNA
0	0	1	5	2	NM_168682.1	CG9704-RA, transcript variant A (Nrt), mRNA
0	0	1	4	3	NM_165849.1	CG8983-RA, transcript variant A (ERp60), mRNA
0	0	1	4	3	NM_136866.2	CG8983-RB, transcript variant B (ERp60), mRNA
0	0	1	3	3	NM_168829.1	CG7433-RB, transcript variant B (CG7433), mRNA
0	0	1	3	3	NM_140911.2	CG7433-RA, transcript variant A (CG7433), mRNA
0	0	1	3	2	NM_001031901.1	CG14209-RC, transcript variant C (Shawn), mRNA
0	0	1	3	2	NM_001031902.1	CG14209-RB, transcript variant B (Shawn), mRNA
0	0	1	3	2	NM_134483.2	CG14208-RB, transcript variant B (Tyler), mRNA
0	0	1	3	2	NM_167671.1	CG14208-RA, transcript variant A (Tyler), mRNA
0	0	1	3	2	NM_001031900.1	CG14209-RD, transcript variant D (Shawn), mRNA
0	0	1	2	5	NM_167535.1	CG9676-RA (CG9676), mRNA
0	0	1	2	4	NM_139683.1	CG7499-RA (Rh50), mRNA
0	0	1	2	4	NM_168379.1	CG32050-RA (CG32050), mRNA
0	0	0	7	7	NM_132788.2	CG9114-RA (CG9114), mRNA
0	0	0	7	3	NM_134517.2	CG15618-RA (CG15618), mRNA
0	0	0	6	5	NM_132789.2	CG6170-RA, transcript variant A (HDAC6), mRNA
0	0	0	6	4	NM_133089.2	CG6659-RA, transcript variant A (CG6659), mRNA
0	0	0	6	4	NM_167627.1	CG6659-RB, transcript variant B (CG6659), mRNA
0	0	0	5	4	NM_132207.2	CG1571-RA (CG1571), mRNA
0	0	0	5	2	NM_079316.2	CG4237-RA (Gap69C), mRNA
0	0	0	5	2	NM_135823.2	CG7311-RA, transcript variant A (CG7311), mRNA

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