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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11734R-1 |
| Symbol |
HERC2 |
Full Name |
HERC2 |
| CG No |
CG11734 |
Old CG No |
CG11734 |
| Synonyms |
CG11734, HERC2 |
| Accession No (Link to NCBI) |
NM_134544.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ibaraki K, Nakatsuka M, Ohsako T, Watanabe M, Miyazaki Y, Shirakami M, Karr TL, Sanuki R, Tomaru M, Takano-Shimizu-Kouno T.
A cross-species approach for the identification of Drosophila male sterility genes.
G3 (Bethesda) (2021) 11(8)
[
PubMed ID = 34849808
]
[
RRC reference
]
Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
PLoS One (2011) 6(9) e24168
[
PubMed ID = 21931660
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGTCTCCGCTGGAGTTGGAGT |
| Primer Seq. 3' |
aatctagaggtaccCCATGTCGGTGGCCTGGCTAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGTCTCCGCT GGAGTTGGAG TAGGAGGGGG TGGAGGTGCC AGTGGAGCAG CGGCAGGAGC
0061 AGGCTCCGCA GCAGGATCTG GATCGGGATC AGGTTCCGGA TCAGCAGCAC CGcCCAGCCA
0121 TTCGCCCGAT CTCTACCTGC GCCCGCTGCC CTTTCTGGAC GCCAAGTGGN TGCGCGCCGA
0181 TCTCATCGCC TCCCTGCGCA ACgCCGCCGA CGGCGCCGTA CTGTGGAACC ATCTGATCCA
0241 GGACTGCGAG CTGGTCTCGT CGCCTACCGC GCCGCTCCTA AATGCCCGCG GACAACTATC
0301 CTACCTGGGC GACGATGGCA AGCACTACTG CGGCGCTCAG CAGCTCAAGT GCTCCTGCTG
0361 CCAGCCGGAG TACTGCGGTC CTCTCTCCGC CTGCAATTGC GNACGGCTGT CGGcCTCTGG
0421 ACTCGGACAC GGCCATCAAA AAGCTTACCA CCCAAgcTGN CGCCCAGCAA GCAGCCCATC
0481 TTANCCAGGC CACCGACATG G |
| in silico PCR Fragment |
0001 AGTCTCCGCT GGAGTTGGAG TAGGAGGGGG TGGAGGTGCC AGTGGAGCAG CGGCAGGAGC
0061 AGGCTCCGCA GCAGGATCTG GATCGGGATC AGGTTCCGGA TCAGCAGCAC CGCCCAGCCA
0121 TTCGCCCGAT CTCTACCTGC GCCCGCTGCC CTTTCTGGAC GCCAAGTGGC TGCGCGCCGA
0181 TCTCATCGCC TCCCTGCGCA ACGCCGCCGA CGGCGCCGTA CTGTGGAACC ATCTGATCCA
0241 GGACTGCGAG CTGGTCTCGT CGCCTACCGC GCCGCTCCTA AATGCCCGCG GACAACTATC
0301 CTACCTGGGC GACGATGGCA AGCACTACTG CGGCGCTCAG CAGCTCAAGT GCTCCTGCTG
0361 CCAGCCGGAG TACTGCGGTC CTCTCTCCGC CTGCAATTGC G-ACGGCTGT CGGCCTCTGG
0421 ACTCGGACAC GGCCATCAAA AAGCTTACCA CCCAAGCTGC CGCCCAGCAA GCAGCCCATC
0481 TTAGCCAGGC CACCGACATG G |
| Assemble Data |
11734R-1.IR_full 1 AGTCTCCGCTGGAGTTGGAGTAGGAGGGGGTGGAGGTGCCAGTGGAGCAGCGGCAGGAGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11734R-1.in silico 1 AGTCTCCGCTGGAGTTGGAGTAGGAGGGGGTGGAGGTGCCAGTGGAGCAGCGGCAGGAGC 60
11734R-1.IR_full 61 AGGCTCCGCAGCAGGATCTGGATCGGGATCAGGTTCCGGATCAGCAGCACCGCCCAGCCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11734R-1.in silico 61 AGGCTCCGCAGCAGGATCTGGATCGGGATCAGGTTCCGGATCAGCAGCACCGCCCAGCCA 120
11734R-1.IR_full 121 TTCGCCCGATCTCTACCTGCGCCCGCTGCCCTTTCTGGACGCCAAGTGGNTGCGCGCCGA 180
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
11734R-1.in silico 121 TTCGCCCGATCTCTACCTGCGCCCGCTGCCCTTTCTGGACGCCAAGTGGCTGCGCGCCGA 180
11734R-1.IR_full 181 TCTCATCGCCTCCCTGCGCAACGCCGCCGACGGCGCCGTACTGTGGAACCATCTGATCCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11734R-1.in silico 181 TCTCATCGCCTCCCTGCGCAACGCCGCCGACGGCGCCGTACTGTGGAACCATCTGATCCA 240
11734R-1.IR_full 241 GGACTGCGAGCTGGTCTCGTCGCCTACCGCGCCGCTCCTAAATGCCCGCGGACAACTATC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11734R-1.in silico 241 GGACTGCGAGCTGGTCTCGTCGCCTACCGCGCCGCTCCTAAATGCCCGCGGACAACTATC 300
11734R-1.IR_full 301 CTACCTGGGCGACGATGGCAAGCACTACTGCGGCGCTCAGCAGCTCAAGTGCTCCTGCTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11734R-1.in silico 301 CTACCTGGGCGACGATGGCAAGCACTACTGCGGCGCTCAGCAGCTCAAGTGCTCCTGCTG 360
11734R-1.IR_full 361 CCAGCCGGAGTACTGCGGTCCTCTCTCCGCCTGCAATTGCGNACGGCTGTCGGCCTCTGG 420
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
11734R-1.in silico 361 CCAGCCGGAGTACTGCGGTCCTCTCTCCGCCTGCAATTGCG-ACGGCTGTCGGCCTCTGG 420
11734R-1.IR_full 421 ACTCGGACACGGCCATCAAAAAGCTTACCACCCAAGCTGNCGCCCAGCAAGCAGCCCATC 480
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
11734R-1.in silico 421 ACTCGGACACGGCCATCAAAAAGCTTACCACCCAAGCTGCCGCCCAGCAAGCAGCCCATC 480
11734R-1.IR_full 481 TTANCCAGGCCACCGACATGG 501
||| |||||||||||||||||
11734R-1.in silico 481 TTAGCCAGGCCACCGACATGG 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
10 |
NM_134544.1 |
CG11734-RB (HERC2), mRNA |
| 0.41 |
2 |
5 |
11 |
34 |
NM_001038856.1 |
CG8585-RC, transcript variant C (Ih), mRNA |
| 0.41 |
2 |
5 |
11 |
34 |
NM_001038859.1 |
CG8585-RE, transcript variant E (Ih), mRNA |
| 0.41 |
2 |
5 |
11 |
34 |
NM_001038858.1 |
CG8585-RB, transcript variant B (Ih), mRNA |
| 0.41 |
2 |
3 |
2 |
2 |
NM_168252.1 |
CG7892-RB, transcript variant B (nmo), mRNA |
| 0.41 |
2 |
3 |
2 |
2 |
NM_168251.1 |
CG7892-RA, transcript variant A (nmo), mRNA |
| 0.41 |
2 |
3 |
2 |
2 |
NM_168253.2 |
CG7892-RD, transcript variant D (nmo), mRNA |
| 0.41 |
2 |
3 |
2 |
2 |
NM_079243.2 |
CG7892-RC, transcript variant C (nmo), mRNA |
| 0.41 |
2 |
2 |
2 |
2 |
NM_143392.2 |
CG14521-RA (CG14521), mRNA |
| 0 |
0 |
8 |
5 |
35 |
NM_131944.1 |
CG3546-RA (CG3546), mRNA |
| 0 |
0 |
7 |
6 |
43 |
NM_078601.2 |
CG9533-RA (rut), mRNA |
| 0 |
0 |
7 |
4 |
8 |
NM_134952.1 |
CG10017-RA (CG10017), mRNA |
| 0 |
0 |
5 |
5 |
7 |
NM_170421.1 |
CG15506-RB, transcript variant B (CG15506), mRNA |
| 0 |
0 |
5 |
5 |
7 |
NM_143457.1 |
CG15506-RA, transcript variant A (CG15506), mRNA |
| 0 |
0 |
5 |
3 |
6 |
NM_206515.1 |
CG14307-RJ, transcript variant J (fru), mRNA |
| 0 |
0 |
5 |
3 |
5 |
NM_169820.1 |
CG14307-RC, transcript variant C (fru), mRNA |
| 0 |
0 |
5 |
3 |
5 |
NM_169819.1 |
CG14307-RE, transcript variant E (fru), mRNA |
| 0 |
0 |
5 |
3 |
5 |
NM_206516.1 |
CG14307-RI, transcript variant I (fru), mRNA |
| 0 |
0 |
5 |
3 |
5 |
NM_206513.1 |
CG14307-RL, transcript variant L (fru), mRNA |
| 0 |
0 |
5 |
3 |
5 |
NM_206512.1 |
CG14307-RM, transcript variant M (fru), mRNA |
| 0 |
0 |
4 |
6 |
20 |
NM_170340.1 |
CG5643-RF, transcript variant F (wdb), mRNA |
| 0 |
0 |
4 |
6 |
20 |
NM_170337.1 |
CG5643-RB, transcript variant B (wdb), mRNA |
| 0 |
0 |
4 |
6 |
20 |
NM_170338.1 |
CG5643-RD, transcript variant D (wdb), mRNA |
| 0 |
0 |
4 |
6 |
20 |
NM_170341.1 |
CG5643-RG, transcript variant G (wdb), mRNA |
| 0 |
0 |
4 |
6 |
20 |
NM_170336.1 |
CG5643-RA, transcript variant A (wdb), mRNA |
| 0 |
0 |
4 |
6 |
20 |
NM_143312.2 |
CG5643-RC, transcript variant C (wdb), mRNA |
| 0 |
0 |
4 |
6 |
20 |
NM_170339.1 |
CG5643-RE, transcript variant E (wdb), mRNA |
| 0 |
0 |
4 |
4 |
10 |
NM_167047.1 |
CG3125-RB, transcript variant B (l(1)G0060), mRNA |
| 0 |
0 |
4 |
4 |
10 |
NM_132025.2 |
CG3125-RA, transcript variant A (l(1)G0060), mRNA |
| 0 |
0 |
3 |
13 |
29 |
NM_169790.1 |
CG7913-RA, transcript variant A (PP2A-B'), mRNA |
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