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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1172R-3 
 Symbol CG1172  Full Name CG1172 
 CG No CG1172  Old CG No CG1172 
 Synonyms CG1172 
 Accession No (Link to NCBI) NM_141277.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGACGGTCGCAAGGAGATCTGG 
 Primer Seq. 3' 
aatctagaggtaccTCGTGGTTGTAGTGCTCCGTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GACGGTCGCA AGGAGATCTG GGATGCGCTA CGGGCGGCAA CCACCGCGGC CGAGGGTCTT 
0061 GACTTTTCAG ATGGCCCAGG cCATCCTGGA TGGGGCTAAC GTGTCGGTTC CCAATGGCTA 
0121 CCTTACAGAA TGCTACGATG AGCTGGGCAC CCAGTACAAA GTGCCCATCT ACTGTCTGTC 
0181 ATATCCCATA AATATCGTAA AGGAGGAGAA CGGGCGCGAC TCGCCTGCCG AATACTCTGA 
0241 GCCCGTGGAT GGCGGCACCG ATATCTTCTT GAAGCTGCGC ATATCATCGA CCATGACTGA 
0301 TGTTAAACTA CCCGTTTACT CCAAGGACAC CGTGGGGCAG TGCAAGAAAA AGCTTCAGGC 
0361 CGCGGAGGGT GTCGACGCCT GCTGCCAGCG ATGGTTCTAC AGCGGAAAAC TCCTGGGCGA 
0421 CAAGGTGCCA ATCGACGAGT GCAGCATACA CCAGGAGATA TGTGGTGCAG GTGATTGTCA 
0481 ACACGGAGCA CTACAACCAC GA 
 in silico PCR Fragment 
0001 GACGGTCGCA AGGAGATCTG GGATGCGCTA CGGGCGGCAA CCACCGCGGC CGAGGGTCTT 
0061 GAC-TTTCAG ATGGCCCAGG CCATCCTGGA TGGGGCTAAC GTGTCGGTTC CCAATGGCTA 
0121 CCTTACAGAA TGCTACGATG AGCTGGGCAC CCAGTACAAA GTGCCCATCT ACTGTCTGTC 
0181 ATATCCCATA AATATCGTAA AGGAGGAGAA CGGGCGCGAC TCGCCTGCCG AATACTCTGA 
0241 GCCCGTGGAT GGCGGCACCG ATATCTTCTT GAAGCTGCGC ATATCATCGA CCATGACTGA 
0301 TGTTAAACTA CCCGTTTACT CCAAGGACAC CGTGGGGCAG TGCAAGAAAA AGCTTCAGGC 
0361 CGCGGAGGGT GTCGACGCCT GCTGCCAGCG ATGGTTCTAC AGCGGAAAAC TCCTGGGCGA 
0421 CAAGGTGCCA ATCGACGAGT GCAGCATACA CCAGG-GATA TGTGGTGCAG GTGATTGTCA 
0481 ACACGGAGCA CTACAACCAC GA 
 Assemble Data 

1172R-3.IR_full       1   GACGGTCGCAAGGAGATCTGGGATGCGCTACGGGCGGCAACCACCGCGGCCGAGGGTCTT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1172R-3.in silico     1   GACGGTCGCAAGGAGATCTGGGATGCGCTACGGGCGGCAACCACCGCGGCCGAGGGTCTT 60


1172R-3.IR_full       61  GACTTTTCAGATGGCCCAGGCCATCCTGGATGGGGCTAACGTGTCGGTTCCCAATGGCTA 120
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1172R-3.in silico     61  GAC-TTTCAGATGGCCCAGGCCATCCTGGATGGGGCTAACGTGTCGGTTCCCAATGGCTA 120


1172R-3.IR_full       121 CCTTACAGAATGCTACGATGAGCTGGGCACCCAGTACAAAGTGCCCATCTACTGTCTGTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1172R-3.in silico     121 CCTTACAGAATGCTACGATGAGCTGGGCACCCAGTACAAAGTGCCCATCTACTGTCTGTC 180


1172R-3.IR_full       181 ATATCCCATAAATATCGTAAAGGAGGAGAACGGGCGCGACTCGCCTGCCGAATACTCTGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1172R-3.in silico     181 ATATCCCATAAATATCGTAAAGGAGGAGAACGGGCGCGACTCGCCTGCCGAATACTCTGA 240


1172R-3.IR_full       241 GCCCGTGGATGGCGGCACCGATATCTTCTTGAAGCTGCGCATATCATCGACCATGACTGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1172R-3.in silico     241 GCCCGTGGATGGCGGCACCGATATCTTCTTGAAGCTGCGCATATCATCGACCATGACTGA 300


1172R-3.IR_full       301 TGTTAAACTACCCGTTTACTCCAAGGACACCGTGGGGCAGTGCAAGAAAAAGCTTCAGGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1172R-3.in silico     301 TGTTAAACTACCCGTTTACTCCAAGGACACCGTGGGGCAGTGCAAGAAAAAGCTTCAGGC 360


1172R-3.IR_full       361 CGCGGAGGGTGTCGACGCCTGCTGCCAGCGATGGTTCTACAGCGGAAAACTCCTGGGCGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1172R-3.in silico     361 CGCGGAGGGTGTCGACGCCTGCTGCCAGCGATGGTTCTACAGCGGAAAACTCCTGGGCGA 420


1172R-3.IR_full       421 CAAGGTGCCAATCGACGAGTGCAGCATACACCAGGAGATATGTGGTGCAGGTGATTGTCA 480
                          ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
1172R-3.in silico     421 CAAGGTGCCAATCGACGAGTGCAGCATACACCAGG-GATATGTGGTGCAGGTGATTGTCA 480


1172R-3.IR_full       481 ACACGGAGCACTACAACCACGA 502
                          ||||||||||||||||||||||
1172R-3.in silico     481 ACACGGAGCACTACAACCACGA 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_141277.2  CG1172-RA (CG1172), mRNA 
0   0  1  4  7  NM_140091.2  CG6757-RA (SH3PX1), mRNA 
0   0  1  4  2  NM_057269.2  CG10236-RA (LanA), mRNA 
0   0  0  5  7  NM_132543.1  CG10362-RA (CG10362), mRNA 
0   0  0  5  5  NM_057470.3  CG9191-RA (Klp61F), mRNA 
0   0  0  5  5  NM_140715.2  CG7692-RA (CG7692), mRNA 
0   0  0  5  5  NM_205912.1  CG11020-RB, transcript variant B (nompC), mRNA 
0   0  0  5  5  NM_078759.2  CG11020-RA, transcript variant A (nompC), mRNA 
0   0  0  4  5  NM_137943.2  CG3493-RA (CG3493), mRNA 
0   0  0  4  4  NM_165399.1  CG1416-RC, transcript variant C (CG1416), mRNA 
0   0  0  4  4  NM_165398.1  CG1416-RB, transcript variant B (CG1416), mRNA 
0   0  0  4  4  NM_136277.2  CG1416-RA, transcript variant A (CG1416), mRNA 
0   0  0  4  3  NM_141852.1  CG12594-RA (CG12594), mRNA 
0   0  0  4  2  NM_057941.3  CG5227-RA, transcript variant A (sdk), mRNA 
0   0  0  4  2  NM_134314.2  CG5227-RB, transcript variant B (sdk), mRNA 
0   0  0  4  2  NM_134315.2  CG5227-RD, transcript variant D (sdk), mRNA 
0   0  0  4  2  NM_057942.3  CG5227-RC, transcript variant C (sdk), mRNA 
0   0  0  4  2  NM_078833.2  CG17212-RA, transcript variant A (rho-6), mRNA 
0   0  0  4  2  NM_176024.1  CG17212-RB, transcript variant B (rho-6), mRNA 
0   0  0  3  6  NM_143453.1  CG7582-RA (CG7582), mRNA 
0   0  0  3  6  NM_136667.2  CG1794-RA, transcript variant A (Mmp2), mRNA 
0   0  0  3  5  NM_136094.1  CG10493-RA (CG10493), mRNA 
0   0  0  3  4  NM_001038943.1  CG4174-RB, transcript variant B (CG4174), mRNA 
0   0  0  3  4  NM_140719.2  CG3885-RA (CG3885), mRNA 
0   0  0  3  4  NM_137087.2  CG12366-RA (O-fut1), mRNA 
0   0  0  3  4  NM_001038941.1  CG13380-RB (CG13380), mRNA 
0   0  0  3  4  NM_079197.4  CG15009-RA, transcript variant A (ImpL2), mRNA 
0   0  0  3  4  NM_168069.1  CG15009-RB, transcript variant B (ImpL2), mRNA 
0   0  0  3  4  NM_168070.1  CG15009-RC, transcript variant C (ImpL2), mRNA 
0   0  0  3  4  NM_169563.1  CG3041-RA (Orc2), mRNA 

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