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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11526R-2 
 Symbol CG11526  Full Name CG11526 
 CG No CG11526  Old CG No CG11526 
 Synonyms CG11526 
 Accession No (Link to NCBI) NM_168023.1 
 Inserted Chr. lll 
 Insertional Mutation  semi-lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACTCCTTTGAGCAGGACACCC 
 Primer Seq. 3' 
aatctagaggtaccGTCCGTGGAGTCCACAAGCGT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACTCCTTAGA GCAGGACACC CACGATGCGG ACGCCAACTG CGACGGGCCC GACCTGGACT 
0061 TCGTGTACGC GGACGTGGAT GGCTATCAGA ACGAGATCGC TGAACTGTAT AGCTACACGG 
0121 AGTTCAGCGA GTTTCAGATG AACGTGAAGG CCTTCGAGGA CCAGATGGAG CTGTACGACC 
0181 TGCCGCCCAA CTGGCAGNAA GCAGGACTCC GCCTCCCAGC GCGGCATCGT GATGAAGCTG 
0241 GTGGACCAGC TGGAGGTCTC CGATCGATCC TTTCGCATGC AGGCCGCCCG GTGCATACTT 
0301 TACCTGGCCC AAGGCTGCTG GGCGGAGGTT CAGTCGGACG AGGAGCAGCA CCAGATCACC 
0361 AGGGACAATG TGCTGGTGCT CTACCAACTC GGCGTCTTCG CCTCCTTCAT CGACCTGCTC 
0421 AACATGGAGA TCGAAAGCGC CTGCTCTCCG GACATTGTGG CCGTCAAGAT CACTAACGTC 
0481 ACGCTTGTGG ACTCCACGGA C 
 in silico PCR Fragment 
0001 ACTCCTTTGA GCAGGACACC CACGATGCGG ACGCCAACTG CGACGGGCCC GACCTGGACT 
0061 TCGTGTACGC GGACGTGGAT GGCTATCAGA ACGAGATCGC TGAACTGTAT AGCTACACGG 
0121 AGTTCAGCGA GTTTCAGATG AACGTGAAGG CCTTCGAGGA CCAGATGGAG CTGTACGACC 
0181 TGCCGCCCAA CTGGCAG-AA GCAGGACTCC GCCTCCCAGC GCGGCATCGT GATGAAGCTG 
0241 GTGGACCAGC TGGAGGTCTC CGATCGATCC TTTCGCATGC AGGCCGCCCG GTGCATCCTT 
0301 TACCTGGCCC AAGGCTGCTG GGCGGAGGTT CAGTCGGACG AGGAGCAGCA CCAGATCACC 
0361 AGGGACAATG TGCTGGTGCT CTACCAACTC GGCGTCTTCG CCTCCTTCAT CGACCTGCTC 
0421 AACATGGAGA TCGAAAGCGC CTGCTCTCCG GACATTGTGG CCGTCAAGAT CACTAACGTC 
0481 ACGCTTGTGG ACTCCACGGA C 
 Assemble Data 

11526R-2.IR_full       1   ACTCCTTAGAGCAGGACACCCACGATGCGGACGCCAACTGCGACGGGCCCGACCTGGACT 60
                           ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
11526R-2.in silico     1   ACTCCTTTGAGCAGGACACCCACGATGCGGACGCCAACTGCGACGGGCCCGACCTGGACT 60


11526R-2.IR_full       61  TCGTGTACGCGGACGTGGATGGCTATCAGAACGAGATCGCTGAACTGTATAGCTACACGG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11526R-2.in silico     61  TCGTGTACGCGGACGTGGATGGCTATCAGAACGAGATCGCTGAACTGTATAGCTACACGG 120


11526R-2.IR_full       121 AGTTCAGCGAGTTTCAGATGAACGTGAAGGCCTTCGAGGACCAGATGGAGCTGTACGACC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11526R-2.in silico     121 AGTTCAGCGAGTTTCAGATGAACGTGAAGGCCTTCGAGGACCAGATGGAGCTGTACGACC 180


11526R-2.IR_full       181 TGCCGCCCAACTGGCAGNAAGCAGGACTCCGCCTCCCAGCGCGGCATCGTGATGAAGCTG 240
                           ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
11526R-2.in silico     181 TGCCGCCCAACTGGCAG-AAGCAGGACTCCGCCTCCCAGCGCGGCATCGTGATGAAGCTG 240


11526R-2.IR_full       241 GTGGACCAGCTGGAGGTCTCCGATCGATCCTTTCGCATGCAGGCCGCCCGGTGCATACTT 300
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
11526R-2.in silico     241 GTGGACCAGCTGGAGGTCTCCGATCGATCCTTTCGCATGCAGGCCGCCCGGTGCATCCTT 300


11526R-2.IR_full       301 TACCTGGCCCAAGGCTGCTGGGCGGAGGTTCAGTCGGACGAGGAGCAGCACCAGATCACC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11526R-2.in silico     301 TACCTGGCCCAAGGCTGCTGGGCGGAGGTTCAGTCGGACGAGGAGCAGCACCAGATCACC 360


11526R-2.IR_full       361 AGGGACAATGTGCTGGTGCTCTACCAACTCGGCGTCTTCGCCTCCTTCATCGACCTGCTC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11526R-2.in silico     361 AGGGACAATGTGCTGGTGCTCTACCAACTCGGCGTCTTCGCCTCCTTCATCGACCTGCTC 420


11526R-2.IR_full       421 AACATGGAGATCGAAAGCGCCTGCTCTCCGGACATTGTGGCCGTCAAGATCACTAACGTC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11526R-2.in silico     421 AACATGGAGATCGAAAGCGCCTGCTCTCCGGACATTGTGGCCGTCAAGATCACTAACGTC 480


11526R-2.IR_full       481 ACGCTTGTGGACTCCACGGAC 501
                           |||||||||||||||||||||
11526R-2.in silico     481 ACGCTTGTGGACTCCACGGAC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_168023.1  CG11526-RA, transcript variant A (CG11526), mRNA 
97.3   469  1  1  6  NM_139549.1  CG11526-RB, transcript variant B (CG11526), mRNA 
0.41   2  3  3  5  NM_168578.1  CG17285-RB, transcript variant B (Fbp1), mRNA 
0.41   2  3  3  5  NM_079341.1  CG17285-RA, transcript variant A (Fbp1), mRNA 
0   0  3  4  2  NM_079939.2  CG5580-RA, transcript variant A (sbb), mRNA 
0   0  3  4  2  NM_206161.1  CG5580-RB, transcript variant B (sbb), mRNA 
0   0  3  4  2  NM_206162.1  CG5580-RC, transcript variant C (sbb), mRNA 
0   0  2  5  4  NM_132539.2  CG1559-RA (Upf1), mRNA 
0   0  2  5  2  NM_170633.1  CG3982-RA (CG3982), mRNA 
0   0  2  3  6  NM_141926.1  CG17639-RA (CG17639), mRNA 
0   0  2  3  4  NM_137801.2  CG6203-RA, transcript variant A (Fmr1), mRNA 
0   0  2  3  4  NM_169324.1  CG6203-RC, transcript variant C (Fmr1), mRNA 
0   0  2  3  4  NM_169325.1  CG6203-RD, transcript variant D (Fmr1), mRNA 
0   0  2  3  4  NM_169326.1  CG6203-RE, transcript variant E (Fmr1), mRNA 
0   0  2  3  3  NM_169327.1  CG6203-RB, transcript variant B (Fmr1), mRNA 
0   0  2  2  5  NM_132413.1  CG15295-RA (CG15295), mRNA 
0   0  1  5  8  NM_206801.1  CG1391-RD, transcript variant D (sol), mRNA 
0   0  1  5  8  NM_057389.4  CG1391-RA, transcript variant A (sol), mRNA 
0   0  1  5  8  NM_206802.1  CG1391-RC, transcript variant C (sol), mRNA 
0   0  1  5  8  NM_057390.4  CG1391-RB, transcript variant B (sol), mRNA 
0   0  1  5  7  NM_135109.1  CG14006-RA (CG14006), mRNA 
0   0  1  5  3  NM_166885.1  CG32813-RD, transcript variant D (CG32813), mRNA 
0   0  1  5  3  NM_166882.1  CG32813-RC, transcript variant C (CG32813), mRNA 
0   0  1  5  3  NM_130553.2  CG32813-RA, transcript variant A (CG32813), mRNA 
0   0  1  5  3  NM_166883.1  CG32813-RF, transcript variant F (CG32813), mRNA 
0   0  1  5  3  NM_166884.2  CG32813-RB, transcript variant B (CG32813), mRNA 
0   0  1  5  3  NM_166881.1  CG32813-RE, transcript variant E (CG32813), mRNA 
0   0  1  4  2  NM_137615.2  CG11048-RA (CG11048), mRNA 
0   0  1  3  5  NM_080005.2  CG3129-RA (Rab-RP4), mRNA 
0   0  1  3  4  NM_206294.1  CG33275-RA, transcript variant A (CG33275), mRNA 

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