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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1150R-2 
 Symbol Osi3  Full Name Osiris 3 
 CG No CG1150  Old CG No CG1150 
 Synonyms osi3, CG1150, Osi3 
 Accession No (Link to NCBI) NM_141365.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACCAGATGATCCGGAGGTCAA 
 Primer Seq. 3' 
aatctagaggtaccTATCGGCGGGCACGTCTACAA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACCAGATGAT CCGGAGGTCA ACCAGCTGCT GTCACGCTCT ATGGGGTCCG ATGAGGAGAC 
0061 CTTCGCCCTT CTGATGGCCA ACAAGCTGTG GAAATTCATT CGTTCGCGCT CCCTGCGATA 
0121 CAAGTTCTCA GAGAACGCGG ACTTTGTGAT CAACAGCGAT CCGGAGGGCA GTCTGAATTT 
0181 GGGCGTATCA GTGCGCCCCC TGGAGGCGTT GCAGGAAGGG CGAGGCAAGA TGAAGAACAT 
0241 GGGGCCACTG CTGATGATGA TGGCGGCCAA GACGGGCATG GTGGGAGCGC TTCTGCTCAA 
0301 AGGACTCTTC CTGCTGGCCG GCAAGGCTCT AATTGTCTCG AAAANTCGCC TTGCTGCTGG 
0361 CGGTGATCAT CTCACTGAAG AAGCTGCTCT CTAGCAAGAA AACCATCGTG GAGGTGCCAT 
0421 CCCACCACGA CAGCTATAGC TCCGGGTGGT CGAGGGCCTT TGACGGGTTC GTGGAGGGAC 
0481 TTGTAGACGT GCCCGCCGAT A 
 in silico PCR Fragment 
0001 ACCAGATGAT CCGGAGGTCA ACCAGCTGCT GTCACGCTCT ATGGGGTCCG ATGAGGAGAC 
0061 CTTCGCCCTT CTGATGGCCA ACAAGCTGTG GAAATTCATT CGTTCGCGCT CCCTGCGATA 
0121 CAAGTTCTCA GAGAACACGG ACTTTGTGAT CAACAGCGAT CCGGAGGGCA GTCTGAATTT 
0181 GGGCGTATCA GTGCGCCCCC TGGAGGCGTT GCAGGAAGGG CGAGGCAAGA TGAAGAACAT 
0241 GGGGCCACTG CTGATGATGA TGGCGGCCAA GACGGGCATG GTGGGAGCGC TTCTGCTCAA 
0301 AGGACTCTTC CTGCTGGCCG GCAAGGCTCT AATTGTCTCG AAAA-TCGCC TTGCTGCTGG 
0361 CGGTGATCAT CTCACTGAAG AAGCTGCTCT CTAGCAAGAA AACCATCGTG GAGGTGCCAT 
0421 CCCACCACGA CAGCTATAGC TCCGGGTGGT CGAGGGCCTT TGACGGGTTC GTGGAGGGAC 
0481 TTGTAGACGT GCCCGCCGAT A 
 Assemble Data 

1150R-2.IR_full       1   ACCAGATGATCCGGAGGTCAACCAGCTGCTGTCACGCTCTATGGGGTCCGATGAGGAGAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1150R-2.in silico     1   ACCAGATGATCCGGAGGTCAACCAGCTGCTGTCACGCTCTATGGGGTCCGATGAGGAGAC 60


1150R-2.IR_full       61  CTTCGCCCTTCTGATGGCCAACAAGCTGTGGAAATTCATTCGTTCGCGCTCCCTGCGATA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1150R-2.in silico     61  CTTCGCCCTTCTGATGGCCAACAAGCTGTGGAAATTCATTCGTTCGCGCTCCCTGCGATA 120


1150R-2.IR_full       121 CAAGTTCTCAGAGAACGCGGACTTTGTGATCAACAGCGATCCGGAGGGCAGTCTGAATTT 180
                          |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
1150R-2.in silico     121 CAAGTTCTCAGAGAACACGGACTTTGTGATCAACAGCGATCCGGAGGGCAGTCTGAATTT 180


1150R-2.IR_full       181 GGGCGTATCAGTGCGCCCCCTGGAGGCGTTGCAGGAAGGGCGAGGCAAGATGAAGAACAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1150R-2.in silico     181 GGGCGTATCAGTGCGCCCCCTGGAGGCGTTGCAGGAAGGGCGAGGCAAGATGAAGAACAT 240


1150R-2.IR_full       241 GGGGCCACTGCTGATGATGATGGCGGCCAAGACGGGCATGGTGGGAGCGCTTCTGCTCAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1150R-2.in silico     241 GGGGCCACTGCTGATGATGATGGCGGCCAAGACGGGCATGGTGGGAGCGCTTCTGCTCAA 300


1150R-2.IR_full       301 AGGACTCTTCCTGCTGGCCGGCAAGGCTCTAATTGTCTCGAAAANTCGCCTTGCTGCTGG 360
                          |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
1150R-2.in silico     301 AGGACTCTTCCTGCTGGCCGGCAAGGCTCTAATTGTCTCGAAAA-TCGCCTTGCTGCTGG 360


1150R-2.IR_full       361 CGGTGATCATCTCACTGAAGAAGCTGCTCTCTAGCAAGAAAACCATCGTGGAGGTGCCAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1150R-2.in silico     361 CGGTGATCATCTCACTGAAGAAGCTGCTCTCTAGCAAGAAAACCATCGTGGAGGTGCCAT 420


1150R-2.IR_full       421 CCCACCACGACAGCTATAGCTCCGGGTGGTCGAGGGCCTTTGACGGGTTCGTGGAGGGAC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1150R-2.in silico     421 CCCACCACGACAGCTATAGCTCCGGGTGGTCGAGGGCCTTTGACGGGTTCGTGGAGGGAC 480


1150R-2.IR_full       481 TTGTAGACGTGCCCGCCGATA 501
                          |||||||||||||||||||||
1150R-2.in silico     481 TTGTAGACGTGCCCGCCGATA 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_141365.2  CG1150-RA (Osi3), mRNA 
0   0  4  4  4  NM_135656.2  CG4851-RA (CG4851), mRNA 
0   0  4  2  4  NM_057388.2  CG9224-RA (sog), mRNA 
0   0  3  5  4  NM_166306.1  CG15093-RA, transcript variant A (CG15093), mRNA 
0   0  3  5  4  NM_166307.2  CG15093-RC, transcript variant C (CG15093), mRNA 
0   0  3  5  4  NM_137529.2  CG15093-RB, transcript variant B (CG15093), mRNA 
0   0  2  4  5  NM_169805.3  CG7125-RD, transcript variant D (PKD), mRNA 
0   0  2  4  5  NM_169804.3  CG7125-RC, transcript variant C (PKD), mRNA 
0   0  2  4  5  NM_169803.3  CG7125-RB, transcript variant B (PKD), mRNA 
0   0  2  4  5  NM_142453.3  CG7125-RA, transcript variant A (PKD), mRNA 
0   0  2  3  4  NM_166075.1  CG30475-RA (CG30475), mRNA 
0   0  2  3  2  NM_137813.1  CG30277-RA (Oatp58Da), mRNA 
0   0  2  2  13  NM_057643.2  CG10844-RA, transcript variant A (Rya-r44F), mRNA 
0   0  2  2  13  NM_057644.2  CG10844-RB, transcript variant B (Rya-r44F), mRNA 
0   0  2  2  13  NM_057645.2  CG10844-RC, transcript variant C (Rya-r44F), mRNA 
0   0  2  2  13  NM_057646.2  CG10844-RD, transcript variant D (Rya-r44F), mRNA 
0   0  2  2  2  NM_079617.3  CG7855-RA (timeout), mRNA 
0   0  2  2  2  NM_168080.1  CG32251-RA (CG32251), mRNA 
0   0  1  5  12  NM_132457.1  CG11122-RA (CG11122), mRNA 
0   0  1  5  4  NM_167617.1  CG32548-RB, transcript variant B (CG32548), mRNA 
0   0  1  5  4  NM_142759.1  CG7084-RA, transcript variant A (CG7084), mRNA 
0   0  1  5  4  NM_169999.1  CG7084-RB, transcript variant B (CG7084), mRNA 
0   0  1  4  15  NM_137081.2  CG30483-RA (Prosap), mRNA 
0   0  1  4  2  NM_135391.2  CG13095-RA (CG13095), mRNA 
0   0  1  2  11  NM_206213.1  CG4527-RD, transcript variant D (slik), mRNA 
0   0  1  2  11  NM_206214.1  CG4527-RC, transcript variant C (slik), mRNA 
0   0  1  2  11  NM_166669.1  CG4527-RB, transcript variant B (slik), mRNA 
0   0  1  2  9  NM_001014549.1  CG4527-RE, transcript variant E (slik), mRNA 
0   0  1  2  7  NM_139397.2  CG18173-RA (CG18173), mRNA 
0   0  1  2  4  NM_138064.2  CG4527-RA, transcript variant A (slik), mRNA 

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