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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1147R-1 |
| Symbol |
NPFR1 |
Full Name |
neuropeptide F receptor |
| CG No |
CG1147 |
Old CG No |
CG1147 |
| Synonyms |
npfr1, CG1147, NPFR, dNPFR1, anon-WO0131005.5, anon-WO0131005.3, NPFR1 |
| Accession No (Link to NCBI) |
NM_079521.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCCTGGTTGTTATAGCCGTCA |
| Primer Seq. 3' |
aatctagaggtaccCAGGTATTGTACGCACAGCGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCCTGGTTGT TATAGCCGTC ATCCGGAAGC CCATCATGCG CACTGCTCGC AATCTGTTCA
0061 TCCTCAACCT GGCCATATCG GACCTACTTT TATGCCTAGT CACCATGCCG CTGNACCTTG
0121 ATGGAGATCC TGTCCAAGTA CTGGCCCTAC TGGCTCCTGC TCCATCCTGT GCAAAACGAT
0181 TGCCATGCTG CAGGCACTTT GTATTTTCGT GTCGACAATA TCCATATACG GCCATTGCCT
0241 TCGACAGATA TCAGGTGATC GTGTANCCCC ACGCGGGACA GCCTGCAGTT CGTGGGCGCC
0301 GGTGACGATC CTGGCGGGGA TCTGGGCACT GGCACTGCTG CTGGCCTCGC CGCTGTTCGT
0361 CTACAAGGAG CTGATCAACA CAGACACGCC GGCACTCCTG CAGCAGATCG GCCTGCAGGA
0421 CACGATCCCG TACTGCATTG AGGACTGGCC AAGTCGCAAN GGGCGCTTCT ACTACTCGAT
0481 CTTCTcNCTG TGCGTACAAT ACCNG |
| in silico PCR Fragment |
0001 CCCTGGTTGT TATAGCCGTC ATCCGGAAGC CCATCATGCG CACTGCTCGC AATCTGTTCA
0061 TCCTCAACCT GGCCATATCG GACCTACTTT TATGCCTAGT CACCATGCCG CTG-ACCTTG
0121 ATGGAGATCC TGTCCAAGTA CTGGCCCTAC -GGCTCCTGC TCCATCCTGT GCAAAACGAT
0181 TGCCATGCTG CAGGCACTTT GTATTTTCGT GTCGACAATA TCCATA-ACG GCCATTGCCT
0241 TCGACAGATA TCAGGTGATC GTGTA-CCCC ACGCGGGACA GCCTGCAGTT CGTGGGCG-C
0301 GGTGACGATC CTGGCGGGGA TCTGGGCACT GGCACTGCTG CTGGCCTCGC CGCTGTTCGT
0361 CTACAAGGAG CTGATCAACA CAGACACGCC GGCACTCCTG CAGCAGATCG GCCTGCAGGA
0421 CACGATCCCG TACTGCATTG AGGACTGGCC AAGTCGCAAC GGGCGCTTCT ACTACTCGAT
0481 CTTCTCGCTG TGCGTACAAT ACCTG |
| Assemble Data |
1147R-1.IR_full 1 CCCTGGTTGTTATAGCCGTCATCCGGAAGCCCATCATGCGCACTGCTCGCAATCTGTTCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1147R-1.in silico 1 CCCTGGTTGTTATAGCCGTCATCCGGAAGCCCATCATGCGCACTGCTCGCAATCTGTTCA 60
1147R-1.IR_full 61 TCCTCAACCTGGCCATATCGGACCTACTTTTATGCCTAGTCACCATGCCGCTGNACCTTG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
1147R-1.in silico 61 TCCTCAACCTGGCCATATCGGACCTACTTTTATGCCTAGTCACCATGCCGCTG-ACCTTG 120
1147R-1.IR_full 121 ATGGAGATCCTGTCCAAGTACTGGCCCTACTGGCTCCTGCTCCATCCTGTGCAAAACGAT 180
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
1147R-1.in silico 121 ATGGAGATCCTGTCCAAGTACTGGCCCTAC-GGCTCCTGCTCCATCCTGTGCAAAACGAT 180
1147R-1.IR_full 181 TGCCATGCTGCAGGCACTTTGTATTTTCGTGTCGACAATATCCATATACGGCCATTGCCT 240
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
1147R-1.in silico 181 TGCCATGCTGCAGGCACTTTGTATTTTCGTGTCGACAATATCCATA-ACGGCCATTGCCT 240
1147R-1.IR_full 241 TCGACAGATATCAGGTGATCGTGTANCCCCACGCGGGACAGCCTGCAGTTCGTGGGCGCC 300
||||||||||||||||||||||||| |||||||||||||||||||||||||||||||| |
1147R-1.in silico 241 TCGACAGATATCAGGTGATCGTGTA-CCCCACGCGGGACAGCCTGCAGTTCGTGGGCG-C 300
1147R-1.IR_full 301 GGTGACGATCCTGGCGGGGATCTGGGCACTGGCACTGCTGCTGGCCTCGCCGCTGTTCGT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1147R-1.in silico 301 GGTGACGATCCTGGCGGGGATCTGGGCACTGGCACTGCTGCTGGCCTCGCCGCTGTTCGT 360
1147R-1.IR_full 361 CTACAAGGAGCTGATCAACACAGACACGCCGGCACTCCTGCAGCAGATCGGCCTGCAGGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1147R-1.in silico 361 CTACAAGGAGCTGATCAACACAGACACGCCGGCACTCCTGCAGCAGATCGGCCTGCAGGA 420
1147R-1.IR_full 421 CACGATCCCGTACTGCATTGAGGACTGGCCAAGTCGCAANGGGCGCTTCTACTACTCGAT 480
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
1147R-1.in silico 421 CACGATCCCGTACTGCATTGAGGACTGGCCAAGTCGCAACGGGCGCTTCTACTACTCGAT 480
1147R-1.IR_full 481 CTTCTCNCTGTGCGTACAATACCNG 505
|||||| |||||||||||||||| |
1147R-1.in silico 481 CTTCTCGCTGTGCGTACAATACCTG 505
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079521.2 |
CG1147-RA (NPFR1), mRNA |
| 0.62 |
3 |
4 |
9 |
3 |
NM_078681.2 |
CG32540-RA (CCKLR-17D3), mRNA |
| 0 |
0 |
3 |
4 |
6 |
NM_078680.2 |
CG6857-RA (CCKLR-17D1), mRNA |
| 0 |
0 |
3 |
3 |
6 |
NM_176144.1 |
CG33144-RA (CG33144), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_140784.1 |
CG7271-RA (CG7271), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_080143.3 |
CG4216-RA (term), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_142493.2 |
CG7717-RA, transcript variant A (Mekk1), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_169833.1 |
CG7717-RB, transcript variant B (Mekk1), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_143012.1 |
CG5789-RA (CG5789), mRNA |
| 0 |
0 |
2 |
3 |
10 |
NM_142397.1 |
CG14318-RA (CG14318), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_142425.2 |
CG7212-RA (cdm), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_134874.2 |
CG2973-RA (CG2973), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_142601.1 |
CG4845-RA (CG4845), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_130604.2 |
CG17766-RA (CG17766), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_141251.1 |
CG2016-RB (CG2016), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_132522.2 |
CG32662-RA (CG32662), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_144391.1 |
CG18745-RA (CG18745), mRNA |
| 0 |
0 |
1 |
7 |
4 |
NM_057219.3 |
CG9042-RA, transcript variant A (Gpdh), mRNA |
| 0 |
0 |
1 |
7 |
4 |
NM_057217.3 |
CG9042-RB, transcript variant B (Gpdh), mRNA |
| 0 |
0 |
1 |
7 |
4 |
NM_057218.3 |
CG9042-RC, transcript variant C (Gpdh), mRNA |
| 0 |
0 |
1 |
7 |
2 |
NM_168850.2 |
CG5498-RB (CG5498), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_130516.2 |
CG3704-RA (CG3704), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_137294.1 |
CG8317-RA (CG8317), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_132851.2 |
CG8509-RA (CG8509), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_079378.2 |
CG5444-RA, transcript variant A (Taf4), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_176333.1 |
CG5444-RC, transcript variant C (Taf4), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_168651.1 |
CG5444-RB, transcript variant B (Taf4), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_206379.1 |
CG5444-RD, transcript variant D (Taf4), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168311.1 |
CG32031-RA, transcript variant A (Argk), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168312.1 |
CG32031-RB, transcript variant B (Argk), mRNA |
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