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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 11450R-2

Symbol net Full Name net

CG No CG11450 Old CG No CG11450

Synonyms CG11450, Shout, Group IId, shout, net

Accession No (Link to NCBI) NM_080081.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees extra vein

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment balanced with TM6B, Tb

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCGTGATGCGGGTTTTTGTTC

Primer Seq. 3'

aatctagaggtaccGGTCGACACATTGCTTTTTGG

Predicted Fragment Size

IR fragment full Seq

0001 cCGTGAATGC GGNTTTTTGT TCCGCNANTT CGGAAAGCGA AgGNNGAGAc GNCTTGGTCg 
0061 tNGANNACGC GCGCAGTGGC AGTCCCGACA TCAGGTACCA AAAGGAACAG ACTCGGCGGA 
0121 CTCCAAGCCA ATAGNCCTTG TCCGCAAACA AACGCAAGAG TTCGGAACCC TTCAAAGTCG 
0181 TAGGTNTTGA CGACTCCCCA ACTCTAAATC CATGNCTGGN CCTCCGTCCG TCCAGTGCGA 
0241 GTATGAATGC AACTGGTCCG CTAAAGAAAC GCATACGATA CACATCTTNC GCCGATTCGG 
0301 CAGTTGTCCT AACGCCACCA GCCATAGACT CTCCACCGCC CAACAGTTGC ATACCTTCCA 
0361 CGTTGCGCCT GCAGCATGAA ATAATgNCTA ATCCGGCACA TATATATGTT CGNCATCCGG 
0421 GAGTTACGAC TCTTCACAGA TCGCTTGCTG CCCACCCGAG AACAGCTGGA ACCACTCGCA 
0481 TTAGTAACGA CCAAAAAGCa ATGTGTCGAC C

in silico PCR Fragment

0001 CCGTG-ATGC GGGTTTTTGT TCCGCCAGTT CGGAAAGCGA AGGCGGAGAC GACTTGGTCG 
0061 TCGAGCACGC GCGCAGTGGC AGTCCCGACA TCAG--ACCA AAAGGAACAG ACTCGGCGGA 
0121 CTCCAAGCCA ATAGCCCTTG TCCGC-AACA AACGCAAGAG TTCGGAACCC TTCAAAGTCG 
0181 TAGGT-TTGA CGACT-CCCA ACTCTAAATC CATGCCTGG- --TCC-TCCG TCCAGTGCGA 
0241 GTATGAATGC AACTGGTCCG CTAAAGAAAC GCATACGATA CACATCTTCC GCCGATTCGG 
0301 CAGTTGTCCT AACGCCACCA GCCATAGACT CTCCACCGCC CAACAGTTGC ATACCTTCCA 
0361 CGTTGCGCCT GCAGCATGAA ATAATGCCTA ATCCGGCACA TATATATGTT CGCCATCCGG 
0421 GAGTTACGAC TCTTCACAGA TCGCTTGCTG CCCACCC-AG AACAGCTGGA ACCACTCGCA 
0481 TTAGTAACGA CCAAAAAGCA ATGTGTCGAC C

Assemble Data


11450R-2.IR_full       1   CCGTGAATGCGGNTTTTTGTTCCGCNANTTCGGAAAGCGAAGGNNGAGACGNCTTGGTCG 60
                           ||||| |||||| |||||||||||| | |||||||||||||||  |||||| ||||||||
11450R-2.in silico     1   CCGTG-ATGCGGGTTTTTGTTCCGCCAGTTCGGAAAGCGAAGGCGGAGACGACTTGGTCG 60


11450R-2.IR_full       61  TNGANNACGCGCGCAGTGGCAGTCCCGACATCAGGTACCAAAAGGAACAGACTCGGCGGA 120
                           | ||  ||||||||||||||||||||||||||||  ||||||||||||||||||||||||
11450R-2.in silico     61  TCGAGCACGCGCGCAGTGGCAGTCCCGACATCAG--ACCAAAAGGAACAGACTCGGCGGA 120


11450R-2.IR_full       121 CTCCAAGCCAATAGNCCTTGTCCGCAAACAAACGCAAGAGTTCGGAACCCTTCAAAGTCG 180
                           |||||||||||||| |||||||||| ||||||||||||||||||||||||||||||||||
11450R-2.in silico     121 CTCCAAGCCAATAGCCCTTGTCCGC-AACAAACGCAAGAGTTCGGAACCCTTCAAAGTCG 180


11450R-2.IR_full       181 TAGGTNTTGACGACTCCCCAACTCTAAATCCATGNCTGGNCCTCCGTCCGTCCAGTGCGA 240
                           ||||| ||||||||| |||||||||||||||||| ||||   ||| ||||||||||||||
11450R-2.in silico     181 TAGGT-TTGACGACT-CCCAACTCTAAATCCATGCCTGG---TCC-TCCGTCCAGTGCGA 240


11450R-2.IR_full       241 GTATGAATGCAACTGGTCCGCTAAAGAAACGCATACGATACACATCTTNCGCCGATTCGG 300
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
11450R-2.in silico     241 GTATGAATGCAACTGGTCCGCTAAAGAAACGCATACGATACACATCTTCCGCCGATTCGG 300


11450R-2.IR_full       301 CAGTTGTCCTAACGCCACCAGCCATAGACTCTCCACCGCCCAACAGTTGCATACCTTCCA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11450R-2.in silico     301 CAGTTGTCCTAACGCCACCAGCCATAGACTCTCCACCGCCCAACAGTTGCATACCTTCCA 360


11450R-2.IR_full       361 CGTTGCGCCTGCAGCATGAAATAATGNCTAATCCGGCACATATATATGTTCGNCATCCGG 420
                           |||||||||||||||||||||||||| ||||||||||||||||||||||||| |||||||
11450R-2.in silico     361 CGTTGCGCCTGCAGCATGAAATAATGCCTAATCCGGCACATATATATGTTCGCCATCCGG 420


11450R-2.IR_full       421 GAGTTACGACTCTTCACAGATCGCTTGCTGCCCACCCGAGAACAGCTGGAACCACTCGCA 480
                           ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
11450R-2.in silico     421 GAGTTACGACTCTTCACAGATCGCTTGCTGCCCACCC-AGAACAGCTGGAACCACTCGCA 480


11450R-2.IR_full       481 TTAGTAACGACCAAAAAGCAATGTGTCGACC 511
                           |||||||||||||||||||||||||||||||
11450R-2.in silico     481 TTAGTAACGACCAAAAAGCAATGTGTCGACC 511

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_080081.2	CG11450-RA (net), mRNA
0	0	3	2	5	NM_164402.1	CG4375-RA (CG4375), mRNA
0	0	2	3	2	NM_143469.1	CG7837-RA (CG7837), mRNA
0	0	2	2	2	NM_165223.1	CG6433-RB, transcript variant B (qua), mRNA
0	0	2	2	2	NM_165222.1	CG6433-RA, transcript variant A (qua), mRNA
0	0	1	3	6	NM_140963.1	CG5847-RA (CG5847), mRNA
0	0	1	2	2	NM_132327.1	CG17440-RA (CG17440), mRNA
0	0	1	2	2	NM_168955.1	CG6838-RB, transcript variant B (CG6838), mRNA
0	0	1	2	2	NM_141152.1	CG6838-RA, transcript variant A (CG6838), mRNA
0	0	1	2	2	NM_206293.1	CG33275-RC, transcript variant C (CG33275), mRNA
0	0	1	2	2	NM_206295.1	CG33275-RB, transcript variant B (CG33275), mRNA
0	0	1	2	2	NM_206294.1	CG33275-RA, transcript variant A (CG33275), mRNA
0	0	0	7	4	NM_140458.1	CG17177-RA (CG17177), mRNA
0	0	0	6	93	NM_167403.2	CG32602-RA (CG32602), mRNA
0	0	0	4	10	NM_001042822.1	CG34120-RC, transcript variant C (CG34120), mRNA
0	0	0	4	8	NM_001042821.1	CG34120-RB, transcript variant B (CG34120), mRNA
0	0	0	4	4	NM_132754.2	CG9512-RA (CG9512), mRNA
0	0	0	4	4	NM_166956.3	CG3588-RA, transcript variant A (CG3588), mRNA
0	0	0	4	3	NM_079985.2	CG5206-RA (bon), mRNA
0	0	0	4	3	NM_132935.1	CG9606-RA (Rrp45), mRNA
0	0	0	4	3	NM_140877.2	CG9376-RA (CG9376), mRNA
0	0	0	4	2	NM_057733.3	CG5210-RA (Chit), mRNA
0	0	0	4	2	NM_135741.2	CG5776-RA (CG5776), mRNA
0	0	0	4	2	NM_132263.2	CG12111-RA (CG12111), mRNA
0	0	0	3	5	NM_168815.1	CG8522-RA, transcript variant A (HLH106), mRNA
0	0	0	3	5	NM_168816.1	CG8522-RB, transcript variant B (HLH106), mRNA
0	0	0	3	5	NM_079442.2	CG8522-RC, transcript variant C (HLH106), mRNA
0	0	0	3	4	NM_136828.1	CG13209-RA (sha), mRNA
0	0	0	3	3	NM_167973.1	CG1271-RD, transcript variant D (CG1271), mRNA
0	0	0	3	3	NM_167974.1	CG1271-RB, transcript variant B (CG1271), mRNA

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