NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11427R-1

Symbol

Full Name

ruby

CG No

CG11427

Old CG No

CG11427

Synonyms

cg11427, CG11427, beta3, AP-3beta, rb

Accession No (Link to NCBI)

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Yano H, Yamamoto-Hino M, Awano W, Aoki-Kinoshita KF, Tsuda-Sakurai K, Okano H, Goto S.
Identification of proteasome components required for apical localization of Chaoptin using functional genomics.
J Neurogenet (2012) 26(1) 53-63 [ PubMed ID = 22417167 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAAATGGGACTGGACGTCGAA

Primer Seq. 3'

aatctagaggtaccTTTTGATCCGCGTCCAGAGAA

Predicted Fragment Size

IR fragment full Seq

in silico PCR Fragment

Assemble Data


11427R-1.IR full       1   CAAATGGGACTGGACGTCGAATTCGGAGCGGATCCTGCCAGCGGAGCAGCTTTCTTCCAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11427R-1.in silico     1   CAAATGGGACTGGACGTCGAATTCGGAGCGGATCCTGCCAGCGGAGCAGCTTTCTTCCAA 60


11427R-1.IR full       61  TCCGATGGACGCAAGCATGATGATCTCAAGCAAATGTTGGACAGCAACAAGGATGGACTA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11427R-1.in silico     61  TCCGATGGACGCAAGCATGATGATCTCAAGCAAATGTTGGACAGCAACAAGGATGGACTA 120


11427R-1.IR full       121 AAGCTGGAGGCCATGAAGCGCATCATTGGCATGATTGCCCGCGGACGGGATGCCAGTGAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11427R-1.in silico     121 AAGCTGGAGGCCATGAAGCGCATCATTGGCATGATTGCCCGCGGACGGGATGCCAGTGAT 180


11427R-1.IR full       181 CTGTTCCCGGCAGTCGTCAAGAATGTGGTGTCCAAGAATATCGAGGTCAAGAAGTTGGTC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11427R-1.in silico     181 CTGTTCCCGGCAGTCGTCAAGAATGTGGTGTCCAAGAATATCGAGGTCAAGAAGTTGGTC 240


11427R-1.IR full       241 TACGTCTATTTGGTGCGATACGCCGAGGAGCAGCAGGACTTGGCCCTGCTATCCATTTCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11427R-1.in silico     241 TACGTCTATTTGGTGCGATACGCCGAGGAGCAGCAGGACTTGGCCCTGCTATCCATTTCC 300


11427R-1.IR full       301 ACATTTCAGAGGGCGCTCAAGGATCCCAATCAACTTATTCGCGCCTCGGCTCTGCGAGTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11427R-1.in silico     301 ACATTTCAGAGGGCGCTCAAGGATCCCAATCAACTTATTCGCGCCTCGGCTCTGCGAGTG 360


11427R-1.IR full       361 TTATCCTCCATAAGGGTCAGCATGATTGTGCCAATCGTAATGCTGGCCATTCGGGATAGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11427R-1.in silico     361 TTATCCTCCATAAGGGTCAGCATGATTGTGCCAATCGTAATGCTGGCCATTCGGGATAGC 420


11427R-1.IR full       421 GCCGCCGATTTGAGTCCATATGTGCGGAAAACAGCGGCACATGCCATCCCCAAGTTGTAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11427R-1.in silico     421 GCCGCCGATTTGAGTCCATATGTGCGGAAAACAGCGGCACATGCCATCCCCAAGTTGTAT 480


11427R-1.IR full       481 TCTCTGGACGCGGATCAAAA 500
                           ||||||||||||||||||||
11427R-1.in silico     481 TCTCTGGACGCGGATCAAAA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

Wing Disc

GFP