NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 11379R-2

Symbol CG11379 Full Name CG11379

CG No CG11379 Old CG No CG11379

Synonyms EG:BACR42I17.4, bs31h12.y1, CG11379

Accession No (Link to NCBI) NM_130532.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCCTTGATCATGGCGGTGTT

Primer Seq. 3'

aatctagaggtaccGATGAAGGGTGCGACGCCAGT

Predicted Fragment Size

IR fragment full Seq

0001 GGCCTNTGAT CATGGCGGTG TTGCTTCACT CGGTACTGGG CATCTCGTAC TACTACTACT 
0061 TTCGCGAATC TCCCGGCTCC GCTGCTCCTC TCTTGGGCGC CTGGCTCTTT TCCGCCTGCG 
0121 TGGTGACCCT TCTTCACGGG GTGAGGATGT TTTCAAGTGC CCTGCCCGCC GATCAGCGGT 
0181 TCAGGAATGG GAATCGAAAC CTTCGCGGCA ATGAGTCGTC CTGGGCATCG CTCTTACTGG 
0241 AGACAGGCGT CTGCTGTTTG GTGCTGGATC TGACCCTTAG CAAACTGTGG CATCGCATAG 
0301 AGTCCCTCTG CCAGTGCGCC ATTGTGGGCA TACTCCAGGC CTTGGCCGTC GAGGAGCAAA 
0361 CCTTCCTGGT CTGCGAGTAC TGGACTTTGG GCCTGACTAC CGGCCTGATC GGCGCCTGTC 
0421 TTCTGTGGCT TTCCCTATGG GCCACCGCGT TGCCCCAGAA GCTCCGCTGC GGGATGCTCG 
0481 ACTGGCGTCG CACCCTTCAT C

in silico PCR Fragment

0001 GGCCT-TGAT CATGGCGGTG TTGCTTCACT CGGTACTGGG CATCTCGTAC TACTACTACT 
0061 TTCGCGAATC TCCCGGCTCC GCTGCTCCTC TCTTGGGCGC CTGGCTCTTT TCCGCCTGCG 
0121 TGGTGACCCT TCTTCACGGG GTGAGGATGT TTTCAAGTGC CCTGCCCGCC GATCAGCGGT 
0181 TCAGGAATGG GAATCGAAAC CTTCGCGGCA ATGAGTCGTC CTGGGCATCG CTCTTACTGG 
0241 AGACAGGCGT CTGCTGTTTG GTGCTGGATC TGACCCTTAG CAAACTGTGG CATCGCATAG 
0301 AGTCCCTCTG CCAGTGCGCC ATTGTGGGCA TACTCCAGGC CTTGGCCGTC GAGGAGCAAA 
0361 CCTTCCTGGT CTGCGAGTAC TGGACTTTGG GCCTGACTAC CGGCCTGATC GGCGCATGTC 
0421 TTCTGTGGCT TTCCCTATGG GCCACCGCGT TGCCCCAGAA GCTCCGCTGC GGGATGCTCG 
0481 ACTGGCGTCG CACCCTTCAT C

Assemble Data


11379R-2.IR_full       1   GGCCTNTGATCATGGCGGTGTTGCTTCACTCGGTACTGGGCATCTCGTACTACTACTACT 60
                           ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
11379R-2.in silico     1   GGCCT-TGATCATGGCGGTGTTGCTTCACTCGGTACTGGGCATCTCGTACTACTACTACT 60


11379R-2.IR_full       61  TTCGCGAATCTCCCGGCTCCGCTGCTCCTCTCTTGGGCGCCTGGCTCTTTTCCGCCTGCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11379R-2.in silico     61  TTCGCGAATCTCCCGGCTCCGCTGCTCCTCTCTTGGGCGCCTGGCTCTTTTCCGCCTGCG 120


11379R-2.IR_full       121 TGGTGACCCTTCTTCACGGGGTGAGGATGTTTTCAAGTGCCCTGCCCGCCGATCAGCGGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11379R-2.in silico     121 TGGTGACCCTTCTTCACGGGGTGAGGATGTTTTCAAGTGCCCTGCCCGCCGATCAGCGGT 180


11379R-2.IR_full       181 TCAGGAATGGGAATCGAAACCTTCGCGGCAATGAGTCGTCCTGGGCATCGCTCTTACTGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11379R-2.in silico     181 TCAGGAATGGGAATCGAAACCTTCGCGGCAATGAGTCGTCCTGGGCATCGCTCTTACTGG 240


11379R-2.IR_full       241 AGACAGGCGTCTGCTGTTTGGTGCTGGATCTGACCCTTAGCAAACTGTGGCATCGCATAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11379R-2.in silico     241 AGACAGGCGTCTGCTGTTTGGTGCTGGATCTGACCCTTAGCAAACTGTGGCATCGCATAG 300


11379R-2.IR_full       301 AGTCCCTCTGCCAGTGCGCCATTGTGGGCATACTCCAGGCCTTGGCCGTCGAGGAGCAAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11379R-2.in silico     301 AGTCCCTCTGCCAGTGCGCCATTGTGGGCATACTCCAGGCCTTGGCCGTCGAGGAGCAAA 360


11379R-2.IR_full       361 CCTTCCTGGTCTGCGAGTACTGGACTTTGGGCCTGACTACCGGCCTGATCGGCGCCTGTC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
11379R-2.in silico     361 CCTTCCTGGTCTGCGAGTACTGGACTTTGGGCCTGACTACCGGCCTGATCGGCGCATGTC 420


11379R-2.IR_full       421 TTCTGTGGCTTTCCCTATGGGCCACCGCGTTGCCCCAGAAGCTCCGCTGCGGGATGCTCG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11379R-2.in silico     421 TTCTGTGGCTTTCCCTATGGGCCACCGCGTTGCCCCAGAAGCTCCGCTGCGGGATGCTCG 480


11379R-2.IR_full       481 ACTGGCGTCGCACCCTTCATC 501
                           |||||||||||||||||||||
11379R-2.in silico     481 ACTGGCGTCGCACCCTTCATC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_130532.1	CG11379-RA (CG11379), mRNA
0	0	2	3	2	NM_166877.1	CG32811-RA (CG32811), mRNA
0	0	2	2	5	NM_166417.1	CG30291-RA (CG30291), mRNA
0	0	2	2	2	NM_140155.2	CG7958-RA, transcript variant A (tna), mRNA
0	0	2	2	2	NM_168422.1	CG7958-RB, transcript variant B (tna), mRNA
0	0	1	11	4	NM_001031984.1	CG33720-RB, transcript variant B (Pif1B), mRNA
0	0	1	11	4	NM_176421.1	CG33191-RA (CG33191), mRNA
0	0	1	5	7	NM_079522.2	CG10281-RA (TfIIFalpha), mRNA
0	0	1	4	3	NM_167944.1	CG32302-RA (CG32302), mRNA
0	0	1	4	2	NM_167276.1	CG1938-RB, transcript variant B (Dlic2), mRNA
0	0	1	4	2	NM_167273.1	CG1938-RC, transcript variant C (Dlic2), mRNA
0	0	1	4	2	NM_132466.2	CG1938-RA, transcript variant A (Dlic2), mRNA
0	0	1	4	2	NM_167274.1	CG1938-RD, transcript variant D (Dlic2), mRNA
0	0	1	3	2	NM_001015502.1	CG40440-PA.3 (CG40440), mRNA
0	0	1	2	5	NM_080166.2	CG3766-RA (scat), mRNA
0	0	1	2	4	NM_170210.1	CG11892-RA, transcript variant A (CG11892), mRNA
0	0	1	2	4	NM_170522.2	CG1322-RC, transcript variant C (zfh1), mRNA
0	0	1	2	4	NM_057502.2	CG1322-RB, transcript variant B (zfh1), mRNA
0	0	1	2	4	NM_143115.2	CG11892-RB, transcript variant B (CG11892), mRNA
0	0	1	2	3	NM_143703.2	CG18214-RA, transcript variant A (trio), mRNA
0	0	1	2	3	NM_167847.1	CG18214-RC, transcript variant C (trio), mRNA
0	0	1	2	3	NM_058064.3	CG10206-RA (nop5), mRNA
0	0	1	2	2	NM_144238.1	CG14189-RA (CG14189), mRNA
0	0	1	2	2	NM_079922.3	CG7962-RA (CdsA), mRNA
0	0	1	2	2	NM_141885.2	CG3132-RA (Ect3), mRNA
0	0	1	2	2	NM_132840.4	CG8239-RA (CG8239), mRNA
0	0	0	5	4	NM_176120.2	CG33135-RA, transcript variant A (KCNQ), mRNA
0	0	0	4	10	NM_205987.1	CG32972-RA, transcript variant A (CG32972), mRNA
0	0	0	4	5	NM_140741.2	CG7510-RA (CG7510), mRNA
0	0	0	4	4	NM_136338.2	CG12792-RA (l(2)09851), mRNA

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