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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1133R-3 
 Symbol opa  Full Name odd paired 
 CG No CG1133  Old CG No CG1133 
 Synonyms CG1133, opa, Opa 
 Accession No (Link to NCBI) NM_079504.3 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCAGCAGCAACAGCAGCAGCA 
 Primer Seq. 3' 
aatctagaggtaccGCTGATGGCGCATGTAGCGCA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCAGGCAGCA ACAGCAGCAG CAACTGGAGA TGACCCAACA GCAGCAGCAA CAACAGCAAC 
0061 AACAGCAGCA GCAACAGCAA CAGGATCAGG AATCCGCGGC GGCCACTGCT GCAGCCTATC 
0121 AGAACTCCGG ATATGGGCAC TTTAACAGCT ATGCCTCCCG GGACTTTTTG CTGGGCAGGA 
0181 GGGAGGCCGA GTACGGTGTG GCAGGATCCG CCGGACAGGC CTCCGCCGCA GCGGATTCCA 
0241 TGCTCTTCTC CGGCTTTCCC GCTCAGGCCG CGGAGCTGGG CTCCGGGTTC GGACAACACC 
0301 CCTTCCACAG CCACCATCAC CACCATCAGA TGCGAATGGG CATGGCGGAT CGCTACGCCG 
0361 CCGGCCACCC GTACAACCAC CACGGCAACT TCCCGACGGC GGCAGTGCAC CACCCGGTCG 
0421 TGCACCACCC GTCCCACCAC GCCATGTCCG CGATGCACCC GGCCGGAGCA GGGGCCTTCC 
0481 CTGCGCTACA TGCGCCATCA GGGGACC 
 in silico PCR Fragment 
0001 GCA-GCAGCA ACAGCAGCAG CAACTGGAGA TGACCCAACA GCAGCAGCAA CAACAGCAAC 
0061 AACAGCAGCA GCAACAGCAA CAGGATCAGG AATCCGCGGC GGCCACTGCT GCAGCCTATC 
0121 AGAACTCCGG ATATGGGCAC TTTAACAGCT ATGCCTCCCG GGACTTTTTG CTGGGCAGGA 
0181 GGGAGGCCGA GTACGGTGTG GCAGGATCCG CCGGACAGGC CTCCGCCGCA GCGGATTCCA 
0241 TGCTCTTCTC CGGCTTTCCC GCTCAGGCCG CGGAGCTGGG CTCCGGGTTC GGACAACACC 
0301 CCTTCCACAG CCACCATCAC CACCATCAGA TGCGAATGGG CATGGCGGAT GCCTACGCCG 
0361 CCGGCCACCC GTACAACCAC CACGGCAACT TCCCGACGGC GGCAGTGCAC CACCCGGTCG 
0421 TGCACCACCC GTCCCACCAC GCCATGTCCG CGATGCACCC GGCCGGAGCA GGGGCCTT-C 
0481 CTGCGCTACA TGCGCCATCA G-----C 
 Assemble Data 

1133R-3.IR_full       1   GCAGGCAGCAACAGCAGCAGCAACTGGAGATGACCCAACAGCAGCAGCAACAACAGCAAC 60
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1133R-3.in silico     1   GCA-GCAGCAACAGCAGCAGCAACTGGAGATGACCCAACAGCAGCAGCAACAACAGCAAC 60


1133R-3.IR_full       61  AACAGCAGCAGCAACAGCAACAGGATCAGGAATCCGCGGCGGCCACTGCTGCAGCCTATC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1133R-3.in silico     61  AACAGCAGCAGCAACAGCAACAGGATCAGGAATCCGCGGCGGCCACTGCTGCAGCCTATC 120


1133R-3.IR_full       121 AGAACTCCGGATATGGGCACTTTAACAGCTATGCCTCCCGGGACTTTTTGCTGGGCAGGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1133R-3.in silico     121 AGAACTCCGGATATGGGCACTTTAACAGCTATGCCTCCCGGGACTTTTTGCTGGGCAGGA 180


1133R-3.IR_full       181 GGGAGGCCGAGTACGGTGTGGCAGGATCCGCCGGACAGGCCTCCGCCGCAGCGGATTCCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1133R-3.in silico     181 GGGAGGCCGAGTACGGTGTGGCAGGATCCGCCGGACAGGCCTCCGCCGCAGCGGATTCCA 240


1133R-3.IR_full       241 TGCTCTTCTCCGGCTTTCCCGCTCAGGCCGCGGAGCTGGGCTCCGGGTTCGGACAACACC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1133R-3.in silico     241 TGCTCTTCTCCGGCTTTCCCGCTCAGGCCGCGGAGCTGGGCTCCGGGTTCGGACAACACC 300


1133R-3.IR_full       301 CCTTCCACAGCCACCATCACCACCATCAGATGCGAATGGGCATGGCGGATCGCTACGCCG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||  ||||||||
1133R-3.in silico     301 CCTTCCACAGCCACCATCACCACCATCAGATGCGAATGGGCATGGCGGATGCCTACGCCG 360


1133R-3.IR_full       361 CCGGCCACCCGTACAACCACCACGGCAACTTCCCGACGGCGGCAGTGCACCACCCGGTCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1133R-3.in silico     361 CCGGCCACCCGTACAACCACCACGGCAACTTCCCGACGGCGGCAGTGCACCACCCGGTCG 420


1133R-3.IR_full       421 TGCACCACCCGTCCCACCACGCCATGTCCGCGATGCACCCGGCCGGAGCAGGGGCCTTCC 480
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
1133R-3.in silico     421 TGCACCACCCGTCCCACCACGCCATGTCCGCGATGCACCCGGCCGGAGCAGGGGCCTT-C 480


1133R-3.IR_full       481 CTGCGCTACATGCGCCATCAGGGGACC 507
                          |||||||||||||||||||||     |
1133R-3.in silico     481 CTGCGCTACATGCGCCATCAG-----C 507



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100.82  486  41  134  408  NM_079504.3  CG1133-RA (opa), mRNA 
12.65   61  536  2032  3724  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
12.65   61  536  2032  3724  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
12.65   61  536  2032  3724  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
8.71   42  208  659  1432  NM_135077.2  CG14023-RA (CG14023), mRNA 
7.88   38  125  314  654  NM_206299.1  CG7391-RC, transcript variant C (Clk), mRNA 
7.88   38  125  314  654  NM_170628.1  CG7391-RB, transcript variant B (Clk), mRNA 
7.88   38  125  314  654  NM_001014576.1  CG7391-RD, transcript variant D (Clk), mRNA 
7.88   38  125  314  654  NM_001014575.1  CG7391-RE, transcript variant E (Clk), mRNA 
7.88   38  125  314  654  NM_001014574.1  CG7391-RF, transcript variant F (Clk), mRNA 
7.88   38  125  314  654  NM_079240.2  CG7391-RA, transcript variant A (Clk), mRNA 
6.43   31  212  629  1183  NM_079903.2  CG15319-RB (nej), mRNA 
6.43   31  126  361  737  NM_169386.1  CG17228-RA, transcript variant A (pros), mRNA 
6.43   31  95  339  691  NM_167239.2  CG32677-RA (CG32677), mRNA 
6.01   29  135  357  547  NM_166992.2  CG2904-RA (ec), mRNA 
6.01   29  131  364  780  NM_169259.1  CG9755-RD, transcript variant D (pum), mRNA 
6.01   29  131  364  780  NM_169258.1  CG9755-RA, transcript variant A (pum), mRNA 
6.01   29  131  364  780  NM_079561.2  CG9755-RC, transcript variant C (pum), mRNA 
6.01   29  129  318  505  NM_080487.2  CG10572-RA (Cdk8), mRNA 
6.01   29  108  308  625  NM_079593.3  CG17228-RC, transcript variant C (pros), mRNA 
6.01   29  108  308  625  NM_176459.1  CG17228-RD, transcript variant D (pros), mRNA 
6.01   29  73  191  371  NM_079119.2  CG4354-RA (slbo), mRNA 
5.8   28  216  783  1737  NM_168571.2  CG32133-RA (CG32133), mRNA 
5.8   28  147  392  895  NM_167231.3  CG17255-RB, transcript variant B (CG17255), mRNA 
5.8   28  147  392  895  NM_132403.3  CG17255-RA, transcript variant A (CG17255), mRNA 
5.6   27  222  776  1613  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
5.6   27  222  776  1613  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
5.6   27  116  383  882  NM_168179.1  CG32394-RA (CG32394), mRNA 
5.18   25  179  709  1563  NM_001038734.1  CG16902-RC (Hr4), mRNA 
4.97   24  123  406  951  NM_143409.1  CG11873-RA (CG11873), mRNA 

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